Article
Veterinary Sciences
Zebang Xu, Xinxin Xu, Bin Yang, Yuling Mi, Jiakun Wang
Summary: Research has shown that it is feasible to establish organoids from single rumen epithelial cells, which is a novel in vitro system that may reduce the use of experimental animals and can be used to study host and pathogen interaction.
VETERINARY RESEARCH
(2023)
Article
Engineering, Biomedical
Jianpei Dong, Jianhua Zhou, Hao Tang, Baiqi Chen, Lu Huang
Summary: We propose a laser-guided programmable hydrogel microstructures construction platform for controllable assembly of multiple cellular spheroids into spatially organized multicellular structures, which can be used for in vitro drug evaluation.
Article
Cell Biology
Avin Ee-Hwan Koh, Suresh Kumar Subbiah, Aisha Farhana, Mohammad Khursheed Alam, Pooi Ling Mok
Summary: The study investigated the efficacy of conditioned medium (CM) obtained from transgenic human erythropoietin-expressing MSCs (MSCEPO) in protecting human retinal pigment epithelial cells from sodium iodate (NaIO3)-induced cell death. Results showed that MSC-CMEPO provided an enhanced mitigating effect against NaIO3-induced ARPE-19 cell death compared to MSC-CM alone, suggesting a potential future therapy for treating retinal degenerative diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Kunyan Zhou, Mahdi Amiri, Azam Salari, Yan Yu, Hua Xu, Ursula Seidler, Katerina Nikolovska
Summary: This study investigated the roles of NHE2 and NHE8 in intestinal epithelial cell function, showing that they have distinct functions in cellular proliferation and pH control.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aya Tentaku, Shusaku Kurisu, Kurumi Sejima, Toshiki Nagao, Akira Takahashi, Shigenobu Yonemura
Summary: The basement membrane (BM) is a crucial extracellular matrix that maintains tissue integrity and function. The mechanisms of how stroma-derived macromolecules, such as collagen IV, are transported and assembled during BM formation remained unclear. This study established an in vitro co-culture model and found that fibroblasts proximal to epithelial cells exclusively contribute to collagen IV assembly.
Article
Cell & Tissue Engineering
Liang Ma, Bibek R. Thapa, Jake A. Le Suer, Andrew Tilston-Lunel, Michael J. Herriges, Andrew Berical, Mary Lou Beermann, Feiya Wang, Pushpinder S. Bawa, Anat Kohn, Alexandra B. Ysasi, Hirofumi Kiyokawa, Taylor M. Matte, Scott H. Randell, Xaralabos Varelas, Finn J. Hawkins, Darrell N. Kotton
Summary: In this study, the engraftment of airway epithelial stem cell compartment was successfully demonstrated through intra-airway transplantation of mouse or human primary and pluripotent stem cells. The engrafted cells showed stable morphologic, molecular, and functional phenotypes of airway epithelia for at least two years. This may pave the way for future cell-based therapy for diseases resulting from airway epithelial cell damage or dysfunction.
Article
Immunology
Shinichi Yamamoto, Ako Matsui, Masaki Ohyagi, Chie Kikutake, Yoshihiro Harada, Mana Iizuka-Koga, Mikita Suyama, Akihiko Yoshimura, Minako Ito
Summary: Regulatory T cells (Tregs) suppress immune responses and contribute to tissue repair by interacting with non-immune cells. In this study, a culture method was developed to generate Tregs with brain Treg characteristics in vitro. These induced brain Tregs (iB-Tregs) showed similar gene expression patterns as in vivo brain Tregs and could infiltrate into the brain more readily and alleviate pathological symptoms in Parkinson's disease models. This research contributes to understanding brain Treg development and suggests therapeutic potential for inflammatory brain diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Jessica Rowley, Sara Namvar, Sara Gago, Briony Labram, Paul Bowyer, Malcolm D. Richardson, Sarah E. Herrick
Summary: Aspergillus fumigatus secretes proteases that induce a proinflammatory response in airway epithelial cells in a strain-dependent manner. The production of proinflammatory cytokines is correlated with conidia germination and hyphal extension. Specific protease inhibitors can reduce the induction of IL-6 and IL-8 in response to different A. fumigatus isolates.
Review
Andrology
Ying-Hong Cui, Wei Chen, Si Wu, Cai-Lin Wan, Zuping He
Summary: Infertility affects 10%-15% of couples worldwide, with male infertility accounting for 50% of the cases. In China, there has been a significant decrease in the newborn population by 7.82 million in 2020 compared to 2016. Stem cells, including ESCs, iPSCs, SSCs, and MSCs, have the potential to differentiate into male germ cells, and recent progress has been made in generating male germ cells in vitro from stem cells under specific conditions. This review addresses the current advancements in deriving male germ cells from stem cells and discusses their potential applications in reproductive medicine.
ASIAN JOURNAL OF ANDROLOGY
(2023)
Article
Plant Sciences
Wenpei Chen, Bingqing Lin, Shichuan Xie, Wei Yang, Junli Lin, Zhaojia Li, Yaxian Zhan, Shuhua Gui, Baoqin Lin
Summary: The study demonstrated that NAR eye drops improved retinal function and morphology, protected mice exposed to NaIO3 through modulating the expression of SIRT1, and promoted cell survival and reduced ROS and carbonyl protein levels in ARPE-19 cells. This protective effect of NAR on dAMD is mediated through SIRT1-mediated anti-oxidation.
Article
Multidisciplinary Sciences
Quynh Dang Le, Watchareewan Rodprasert, Suryo Kuncorojakti, Prasit Pavasant, Thanaphum Osathanon, Chenphop Sawangmake
Summary: This study established an effective protocol for generating insulin-producing cells (IPCs) from canine adipose mesenchymal stem cells (cAD-MSCs). The modulated three-stepwise protocol successfully induced functional IPCs. Additionally, the cultivation and preservation of IPCs in a specialized medium improved their maturation.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Junya Tanaka, Hisaaki Takahashi, Hajime Yano, Hiroshi Nakanishi
Summary: This study demonstrates the presence of microglial progenitors expressing EGFR in the leptomeninges, indicating a population of microglia-like cells that grow independently of CSF1. The cells derived from leptomeninges resemble parenchymal microglia more closely than primary microglia.
Article
Biochemistry & Molecular Biology
Nathalie Baumlin, Neerupma Silswal, John S. Dennis, Asef J. Niloy, Michael D. Kim, Matthias Salathe
Summary: Flavorings, such as menthol, in e-cigarettes can affect the clearance of mucus in the airways, potentially leading to harm. Menthol at high concentrations used in e-cigs can decrease ciliary beat frequency, reduce airway surface liquid volume, and increase mucus concentrations, resulting in impaired mucociliary clearance and increased inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Elrade Rofaani, Yong He, Juan Peng, Yong Chen
Summary: This study developed a method to overcome the lack of in vivo-like basement membrane (BM) for in vitro modeling by culturing primordial lung progenitors (PLPs) on an artificial basement membrane (ABM) composed of collagen IV and laminin. The forces generated by the growing epithelial monolayer on the ABM led to geometry-dependent folding and enhanced expression of surfactant protein C and aquaporin 5 compared with a nanofiber-covered substrate. This work emphasizes the importance of recapitulating natural BM for advanced epithelial modeling.
ACTA BIOMATERIALIA
(2023)
Article
Gastroenterology & Hepatology
Mastura Neyazi, Sraddha S. Bharadwaj, Samuel Bullers, Zofia Varenyiova, Simon Travis, Carolina Arancibia-Carcamo, Fiona Powrie, Alessandra Geremia
Summary: Higher expression of OLFM4, a cancer stemness gene induced by IL-22, is present in PSC-UC, suggesting that IL-22 responses may result in alterations of the intestinal stem-cell niche in these patients. IFN gamma stimulation resulted in the downregulation of LGR5 in EpOCs, whereas higher expression of OLFM4 was observed after IL-22 stimulation, indicating a complex interplay between these cytokines in contributing to carcinogenesis in PSC-UC.
INFLAMMATORY BOWEL DISEASES
(2021)
Article
Biophysics
Kristina R. Rivera, Vladimir A. Pozdin, Ashlyn T. Young, Patrick D. Erb, Natalie A. Wisniewski, Scott T. Magness, Michael Daniele
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Gastroenterology & Hepatology
Liara M. Gonzalez, Amy Stieler Stewart, John Freund, Cecilia Renee Kucera, Christopher M. Dekaney, Scott T. Magness, Anthony T. Blikslager
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2019)
Article
Developmental Biology
Jesse R. Raab, Deepthi Y. Tulasi, Kortney E. Wager, Jeremy M. Morowitz, Scott T. Magness, Adam D. Gracz
Article
Multidisciplinary Sciences
Jimin Min, Paige N. Vega, Amy C. Engevik, Janice A. Williams, Qing Yang, Loraine M. Patterson, Alan J. Simmons, R. Jarrett Bliton, Joshua W. Betts, Ken S. Lau, Scott T. Magness, James R. Goldenring, Eunyoung Choi
NATURE COMMUNICATIONS
(2019)
Editorial Material
Engineering, Biomedical
R. Jarrett Bliton, Scott T. Magness
NATURE BIOMEDICAL ENGINEERING
(2020)
Correction
Microbiology
Jason W. Arnold, Jeffery Roach, Salvador Fabela, Emily Moorfield, Shengli Ding, Eric Blue, Suzanne Dagher, Scott Magness, Rita Tamayo, Jose M. Bruno-Barcena, M. Andrea Azcarate-Peril
Article
Microbiology
Jason W. Arnold, Jeffery Roach, Salvador Fabela, Emily Moorfield, Shengli Ding, Eric Blue, Suzanne Dagher, Scott Magness, Rita Tamayo, Jose M. Bruno-Barcena, M. Andrea Azcarate-Peril
Summary: Dietary GOS had a positive impact on the microbiome composition and intestinal health of both old and young animals, but a consistent bifidogenic effect was not observed. Antibiotics had a more significant impact on regulating inflammation markers, while GOS improved the intestinal barrier homeostasis and increased mucus production to promote gut health in the elderly.
Article
Biochemistry & Molecular Biology
Xingqing Xiao, Sudeep Sarma, Stefano Menegatti, Nathan Crook, Scott T. Magness, Carol K. Hall
Summary: In this study, promising toxin A-targeting peptides were identified using a computational strategy that integrates peptide binding design algorithm and molecular dynamics simulation. These peptides may serve as potential inhibitors against C. difficile infection. In vitro experiments showed that phage-display peptide RP and in silico peptide NPA exhibit strong toxin-neutralizing properties, while in silico peptide NPB has relatively lower efficacy against TcdA.
ACS CHEMICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Longping Tse, Rita M. Meganck, Kenza C. Araba, Boyd L. Yount, Kendall M. Shaffer, Yixuan J. Hou, Jennifer E. Munt, Lily E. Adams, Jason A. Wykoff, Jeremy M. Morowitz, Stephanie Dong, Scott T. Magness, William F. Marzluff, Liara M. Gonzalez, Camille Ehre, Ralph S. Baric
Summary: SADS-CoV poses a threat to both human and animal populations, and the host factor PLAC8 has been identified as essential for infection. This conserved infection pathway among mammals could be a promising target for antiviral development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Gastroenterology & Hepatology
Ismael Gomez-Martinez, R. Jarrett Bliton, Keith A. Breau, Michael J. Czerwinski, Ian A. Williamson, Jia Wen, John F. Rawls, Scott T. Magness
Summary: Through single-cell RNA sequencing, it was found that the expression of lipid-handling genes increases as absorptive enterocytes mature. Culture conditions promote differentiation of intestinal stem cells into dense absorptive enterocyte monolayers. Fatty acid oxidation modulation affects fatty acid export, while short-chain fatty acids are unaffected.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Microbiology
Alan J. Marsh, Kshipra Chandrashekhar, Sandy Ng, Jeff Roach, Scott T. Magness, M. Andrea Azcarate-Peril
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)
Article
Microbiology
Alan J. Marsh, Kshipra Chandrashekhar, Sandy Ng, Jeff Roach, Scott T. Magness, M. Andrea Azcarate-Peril
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)
Article
Gastroenterology & Hepatology
Sylvia H. Ferguson, Derek M. Foster, Barbara Sherry, Scott T. Magness, Dahlia M. Nielsen, Jody L. Gookin
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2019)
Article
Gastroenterology & Hepatology
Bailey Zwarycz, Adam D. Gracz, Kristina R. Rivera, Ian A. Williamson, Leigh A. Samsa, Josh Starmer, Michael A. Daniele, Luisa Salter-Cid, Qihong Zhao, Scott T. Magness
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2019)
Article
Gastroenterology & Hepatology
Nicholas R. Smith, John R. Swain, Paige S. Davies, Alexandra C. Gallagher, Michael S. Parappilly, Catherine Z. Beach, Philip R. Streeter, Ian A. Williamson, Scott T. Magness, Melissa H. Wong
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2018)