Article
Entomology
Long Liu, Yu-Shan Wei, Dun Wang
Summary: This study identified and characterized eleven TLRs and five downstream TLR pathway components in Lymantria dispar, and found that upon Lymantria dispar multiple nucleopolyhedrovirus infection, several TLRs and all identified downstream genes of the TLR pathway were significantly up-regulated, suggesting activation of the TLR pathway and its involvement in the insect's immune response.
Article
Multidisciplinary Sciences
Hui-Chung Lin, Der-Jiang Chiao, Chang-Chi Lin, Szu-Cheng Kuo
Summary: The study demonstrates efficient delivery of CHIKV replicons into cells using a baculovirus, verifying its efficacy through experiments, offering insights into virus replication mechanisms and potential for antiviral development.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Margaux Vienne, Marion Etiennot, Bertrand Escaliere, Justine Galluso, Lionel Spinelli, Sophie Guia, Aurore Fenis, Eric Vivier, Yann M. Kerdiles
Summary: NK cells are known to have cytotoxic effects on tumor cell lines, but their specific roles in primary tumor detection and elimination remain unclear. ILC1 play an active role in inhibiting the antitumoral immune response, suggesting the need to evaluate the tumor infiltration of ILC1 and NK cells to optimize immune harnessing in cancer therapies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kornelia Bodo, Zoltan Kellermayer, Zoltan Laszlo, Akos Boros, Bohdana Kokhanyuk, Peter Nemeth, Peter Engelmann
Summary: The study aimed to investigate the mechanisms of innate immunity during regeneration in Eisenia andrei earthworms, revealing dynamic changes in cell proliferation during anterior and posterior regeneration, accumulation of different immune cell subsets in the newly-formed blastema, and downregulation of pattern recognition receptors and antimicrobial peptides mRNA patterns in regenerating earthworms. Experimental coelomocyte depletion significantly impaired cell divisions and blastema formation during regeneration, indicating complex interactions between regeneration and invertebrate innate immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Sabrina Amalfi, Maria del Pilar Plastine, Maria Gabriela Lopez, Maria Jose Gravisaco, Oscar Taboga, Victoria Alfonso
Summary: In this study, researchers demonstrated that the poxin homologous P26 from AcMNPV can degrade 2'3'-cGAMP, thus inhibiting the antiviral response and improving gene delivery efficiency. Incorporating P26 into baculoviral vectors significantly increased the efficiency of transgene expression.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Immunology
Tao Qin, Danni Feng, Bangyue Zhou, Lirong Bai, Shengjie Zhou, Jiangtao Du, Gang Xu, Yinyan Yin
Summary: Melatonin, a hormone with antioxidant and anti-inflammatory properties, can protect dendritic cells (DCs) from acute lung injury (ALI) by restoring body weight, decreasing lung injury, reducing cytokine levels, inhibiting DC activation, and regulating DC migration.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Goekhan Yuecel, Xiaobo Zhou, Linda Terkatz, Angela Wendel, Julius Reinhardt, Ibrahim El-Battrawy, Katherine Sattler, Lukas Cyganek, Jochen Utikal, Harald Langer, Ruediger Scharf, Daniel Duerschmied, Ibrahim Akin
Summary: PAMPs play a role in the pathogenesis of septic cardiomyopathy through TLR-mediated immune response. hiPSC-CMs provide a useful tool for studying PAMP-induced changes in cardiomyocytes. Higher doses of flagellin increase the expression levels of inflammation-associated cytokines and downstream signaling molecules, as well as the expression and fluorescence proportion of TLR5 in hiPSC-CMs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Sneha Mahesh, Jenny Li, Tatianna Travieso, Danai Psaradelli, Donatella Negri, Mary Klotman, Andrea Cara, Maria Blasi
Summary: The choice of vector promoter influences antigen expression levels in target cells and the magnitude of T cell responses in vivo.
Article
Immunology
Hella Pasmans, Magdalena A. Berkowska, Annieck M. Diks, Bas de Mooij, Rick J. Groenland, Lia de Rond, M. Alina Nicolaie, Sjoerd H. van der Burg, Jacques J. M. van Dongen, Fiona R. M. van der Klis, Anne-Marie Buisman
Summary: A study comparing immune responses in women vaccinated with bivalent and nonavalent HPV vaccines found consistent expansion of plasma cells and monocytes, but varied responses in other immune cells. Antibody levels and memory B and T-cell responses also differed between the two vaccine groups.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Amelia Barilli, Rossana Visigalli, Francesca Ferrari, Giulia Recchia Luciani, Maurizio Soli, Valeria Dall'Asta, Bianca Maria Rotoli
Summary: The purpose of this study was to investigate the impact of the JAK-STAT inhibitor baricitinib on the inflammatory response caused by the spike S1 protein from SARS-CoV-2. The research findings indicate that baricitinib can inhibit the phosphorylation of STAT1 and STAT3 in MDM, and decrease the release of IL-6 and TNF alpha. However, its effectiveness on endothelial cell activation is limited.
Article
Cell Biology
Mengdie Li, Fengrun Sun, Yuanyuan Xu, Lanting Chen, Chunqin Chen, Liyuan Cui, Jinfeng Qian, Dajin Li, Songcun Wang, Meirong Du
Summary: This study reveals the significant roles of Tim-3 in regulating macrophage function during pregnancy and its importance in maintaining normal pregnancy. The expression of Tim-3 on decidual macrophages is closely related to immune regulation between decidual cells and promotes pregnancy stability.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Mamoru Matsumura, Mika Nomoto, Tomotaka Itaya, Yuri Aratani, Mizuki Iwamoto, Takakazu Matsuura, Yuki Hayashi, Tsuyoshi Mori, Michael J. Skelly, Yoshiharu Y. Yamamoto, Toshinori Kinoshita, Izumi C. Mori, Takamasa Suzuki, Shigeyuki Betsuyaku, Steven H. Spoel, Masatsugu Toyota, Yasuomi Tada
Summary: Perceiving pathogen signals and environmental cues is crucial for plant immunity. This study reveals that trichomes in plants can sense external mechanical forces, such as raindrops, and induce the expression of defense-related genes through the propagation of intercellular calcium waves, providing early protection against microbial infections.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Xin Liu, Guo-Ping Shi, Junli Guo
Summary: Pressure overload and heart failure are major causes of cardiovascular morbidity and mortality, where innate immune cells and inflammatory mediators play crucial roles. Different innate immune cells, including mast cells, macrophages, monocytes, neutrophils, dendritic cells, eosinophils, and natural killer T cells, exhibit varying levels of activity in these pathological processes, with some exacerbating cardiodysfunction and others displaying cardioprotective activities. Overall, immune regulation of cardiac innate immune cells holds promise as a therapeutic approach in experimental cardiac disease treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Entomology
Ashley L. Waring, Joshua Hill, Brooke M. Allen, Nicholas M. Bretz, Nguyen Le, Pooja Kr, Dakota Fuss, Nathan T. Mortimer
Summary: Summary: This study characterizes the gene expression changes in Drosophila fruit flies following infection by different pathogens and identifies a small number of genes that consistently show expression changes. These findings provide new insight into the transcriptional changes that accompany the immune response in fruit flies and may serve as candidate genes for further study.
Article
Biochemistry & Molecular Biology
Wenjun Guo, Mengmeng Wang, Lei Chen
Summary: The BacMam system, utilizing baculovirus as a delivery tool, is widely used for recombinant production of eukaryotic proteins in mammalian cells. In this study, a BacMam vector (pBMCL1) was developed, which allows easy tracking of virus production, enhances infection efficiency in mammalian cells, reduces unwanted transcription of toxic genes in insect cells, and enables the co-expression of multiple proteins using a single virus. The successful application of the pBMCL1 vector in expressing homo-tetrameric human TRPC3 channel and hetero-octameric KATP channel is demonstrated.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Sup Kim, June-Young Lee, Seul Gi Shin, Jin Kyung Kim, Prashanta Silwal, Young Jae Kim, Na-Ri Shin, Pil Soo Kim, Minho Won, Sang-Hee Lee, Soo Yeon Kim, Miwa Sasai, Masahiro Yamamoto, Jin-Man Kim, Jin-Woo Bae, Eun-Kyeong Jo
Summary: ESRRA plays a critical role in regulating intestinal homeostasis and ameliorating colonic inflammation by activating autophagy pathways and controlling host gut microbiota, protecting the host from detrimental inflammation and dysfunctional mitochondria.
Review
Immunology
Haruna Okude, Daisuke Ori, Taro Kawai
Summary: Recognition of pathogen-derived nucleic acids by PRRs is crucial for antiviral immune responses, but abnormal recognition of self-nucleic acids by PRRs is associated with autoimmune diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Miwa Sasai, Ji Su Ma, Masaaki Okamoto, Kohei Nishino, Hikaru Nagaoka, Eizo Takashima, Ariel Pradipta, Youngae Lee, Hidetaka Kosako, Pann-Ghill Suh, Masahiro Yamamoto
Summary: The TCR signaling in CD8(+) T cells is qualitatively different from that in CD4(+) T cells due to the involvement of PLC beta 4. PLC beta 4 plays a crucial role in activating CD8(+) T cells and promoting adaptive immunity, while deficiency of PLC beta 4 leads to impaired immune responses in CD8(+) T cells.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Yafei Liu, Wai Tuck Soh, Jun-ichi Kishikawa, Mika Hirose, Emi E. Nakayama, Songling Li, Miwa Sasai, Tatsuya Suzuki, Asa Tada, Akemi Arakawa, Sumiko Matsuoka, Kanako Akamatsu, Makoto Matsuda, Chikako Ono, Shiho Torii, Kazuki Kishida, Hui Jin, Wataru Nakai, Noriko Arase, Atsushi Nakagawa, Maki Matsumoto, Yukoh Nakazaki, Yasuhiro Shindo, Masako Kohyama, Keisuke Tomii, Koichiro Ohmura, Shiro Ohshima, Toru Okamoto, Masahiro Yamamoto, Hironori Nakagami, Yoshiharu Matsuura, Atsushi Nakagawa, Takayuki Kato, Masato Okada, Daron M. Standley, Tatsuo Shioda, Hisashi Arase
Summary: The research found that antibodies against different domains of the SARS-CoV-2 spike protein have different effects, with some antibodies inducing an open conformation of the RBD and enhancing the virus infectivity. Structural and mutational analysis revealed the mechanisms of these antibodies, and infectivity-enhancing antibodies were detected at higher levels in severe patients.
Article
Parasitology
Ariel Pradipta, Hironori Bando, Ji Su Ma, Shun Tanaka, Miwa Sasai, Masahiro Yamamoto
Summary: The study reveals that Atg9 and Atg14, despite their essential roles in autophagy, are dispensable for restricting the growth of uis3(-) parasites, while cells lacking LC3 proteins can still limit the growth of uis3(-) parasites. Contrary to previous models, GABARAPs are shown to be involved in suppressing the growth of uis3(-) parasites, independent of autophagy.
PARASITOLOGY INTERNATIONAL
(2021)
Article
Biology
Ariel Pradipta, Miwa Sasai, Kou Motani, Ji Su Ma, Youngae Lee, Hidetaka Kosako, Masahiro Yamamoto
Summary: The interferon-inducible GTPase Irgm2 plays a crucial role in controlling parasite killing through recruitment of Gbp1 and Irgb6, regardless of its localization at the parasitophorous vacuole membranes (PVM) of Toxoplasma. Ubiquitination of Irgm2 in the cytosol is important for parasite killing by recruiting Gbp1 to the PVM. Meanwhile, PVM ubiquitination and loading of p62/Sqstm1 at later time points post-Toxoplasma infection require Irgm2 localization at the PVM.
LIFE SCIENCE ALLIANCE
(2021)
Editorial Material
Immunology
Kouji Kobiyama, Ken J. Ishii
Summary: mRNA vaccines owe their success in part to methylation, which masks the immunostimulatory properties of the mRNA. However, the immunological mechanisms of adjuvanticity are still unclear. Two new studies have revealed distinct mechanisms for the innate sensing of this hidden adjuvant.
Article
Immunology
Akane Watanabe, Sumiyuki Nishida, Temizoz Burcu, Takayuki Shibahara, Takato Kusakabe, Etsushi Kuroda, Ken J. Ishii, Atsushi Kumanogoh
Summary: This study investigated the safety and immunogenicity of a seasonal influenza vaccine adjuvanted with HP-beta-CyD. The results showed that the vaccine was well tolerated and immunogenic in healthy adults, despite containing less HA antigens.
Article
Parasitology
Satomi Yamamoto, Sakure Nakamura, Kazuhiko Nakayama, Kodai Kusakisako, Koh Watanabe, Hiromi Ikadai, Taishi Tanabe
Summary: This study investigated the prevalence of Crithidia mellificae and Lotmaria passim in Apis mellifera in six regions of Japan from 2018 to 2019. The results showed that the detection rate of Crithidia mellificae was 0.0% in all regions, while Lotmaria passim was detected in 16.7%-66.7% of the honeybees. Furthermore, phylogenetic analysis revealed that all the analyzed samples belonged to the Lotmaria passim clade. This study highlights the predominant prevalence of Lotmaria passim infection in Japanese honeybees and suggests the need for further epidemiological surveys on Crithidia mellificae infection.
PARASITOLOGY INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Yuxin Wang, Tatsuya Morishima, Maiko Sezaki, Ryo Sato, Gaku Nakato, Shinji Fukuda, Kouji Kobiyama, Ken J. Ishii, Yuhua Li, Hitoshi Takizawa
Summary: Bacterial infections can activate hematopoietic stem and progenitor cells to migrate from the bone marrow to the spleen, a process known as extramedullary hematopoiesis. Recent studies have shown that gut commensal bacteria not only regulate the host immune system, but also hematopoietic homeostasis. This study reveals that Akkermansia muciniphila, a mucin-degrading bacterium, can rapidly activate bone marrow myelopoiesis and induce delayed extramedullary hematopoiesis through the MYD88/TRIF innate immune signaling pathway.
Article
Immunology
Shunsuke Yamada, Yuichi Kitai, Takashi Tadokoro, Runa Takahashi, Haruka Shoji, Taiga Maemoto, Marie Ishiura, Ryuta Muromoto, Jun-Ichi Kashiwakura, Ken J. Ishii, Katsumi Maenaka, Taro Kawai, Tadashi Matsuda
Summary: Damage-associated molecular patterns (DAMPs) released from cancer cells play a crucial role in activating antitumor immune responses during cancer chemotherapy. This study reveals that topotecan (TPT) inhibits the formation of preribosomal subunits by targeting RPL15, triggering the secretion of DAMPs and enhancing the antitumor immune activation. Ribosomal stress is identified as the key factor in inducing DAMP release, highlighting the potential implications for antitumor immunotherapy.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Burcu Temizoz, Kou Hioki, Shingo Kobari, Nao Jounai, Takato Kusakabe, Michelle S. J. Lee, Cevayir Coban, Etsushi Kuroda, Ken J. Ishii
Summary: Combining TLR9 and STING agonists can synergistically induce IFN-gamma, serving as an effective anti-tumor therapy, particularly important for cancers like pancreatic cancer.
INTERNATIONAL IMMUNOLOGY
(2022)
Article
Immunology
Julia Matsuo-Dapaah, Michelle Sue Jann Lee, Ken J. Ishii, Kazuki Tainaka, Cevayir Coban
Summary: The study utilized a new tissue-clearing method and light-sheet fluorescent microscopy to visualize the spatial distribution of Plasmodium berghei ANKA parasites in the whole-brain microvessels during a mouse model of experimental cerebral malaria. It was found that the parasites predominantly accumulated in the olfactory bulb and brainstem, with a higher accumulation in the absence of the olfactory bulb. This demonstrates the potential of the tissue-clearing technology for visualizing pathological events in the whole brain at the cellular level and advancing our understanding of human cerebral malaria.
INTERNATIONAL IMMUNOLOGY
(2021)
Article
Immunology
Sophia P. M. Sok, Daisuke Ori, Ayana Wada, Haruna Okude, Takumi Kawasaki, Masatoshi Momota, Noor Hasima Nagoor, Taro Kawai
Summary: The natural compound 1'-Acetoxychavicol acetate (ACA) was found to inhibit NLRP3 inflammasome activation in mouse and human cells, along with suppression of molecules and oxidative processes associated with NLRP3 activation. In mouse models, ACA also showed efficacy in preventing NLRP3 inflammasome activation.
INTERNATIONAL IMMUNOLOGY
(2021)
Article
Veterinary Sciences
Taishi Tanabe, Yurina Fukuda, Kazuhiko Kawashima, Satomi Yamamoto, Takashige Kashimoto, Hisaaki Sato
Summary: The study found that alpha-amanitin has antiviral activity against feline immunodeficiency virus (FIV) by inhibiting the RNA transcription level.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2021)