Mechanosensory trichome cells evoke a mechanical stimuli-induced immune response in Arabidopsis thaliana

Perception of pathogen-derived ligands by corresponding host receptors is a pivotal strategy in eukaryotic innate immunity. In plants, this is complemented by circadian anticipation of infection timing, promoting basal resistance even in the absence of pathogen threat. Here, we report that trichomes, hair-like structures on the epidermis, directly sense external mechanical forces, including raindrops, to anticipate pathogen infections in Arabidopsis thaliana. Exposure of leaf surfaces to mechanical stimuli initiates the concentric propagation of intercellular calcium waves away from trichomes to induce defence-related genes. Propagating calcium waves enable effective immunity against pathogenic microbes through the CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 3 (CAMTA3) and mitogen-activated protein kinases. We propose an early layer of plant immunity in which trichomes function as mechanosensory cells that detect potential risks. Plant immunity can be induced by pathogen signals or environmental cues. Here, the authors show that plant leaves use trichomes to sense incoming raindrops and trigger basal defence responses to protect against subsequent microbial infection.

Automated summary

Perceiving pathogen signals and environmental cues is crucial for plant immunity. This study reveals that trichomes in plants can sense external mechanical forces, such as raindrops, and induce the expression of defense-related genes through the propagation of intercellular calcium waves, providing early protection against microbial infections.