Article
Virology
Shimin Li, Dianbing Wang, Abbas Ghulam, Xia Li, Min Li, Qin Li, Yingxin Ma, Lei Wang, Hangshen Wu, Zongqiang Cui, Xian-En Zhang
Summary: A genetically engineered replication-competent ZIKV with fluorescent labeling has been successfully generated using the tetracysteine (TC) tag system. This fluorescent labeling strategy allows for tracking the interactions between ZIKV and the host at both the protein and viral particle level.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Sofia Romero, Nuruddin Unchwaniwala, Edward L. Evans III, Kevin W. Eliceiri, Daniel D. Loeb, Nathan M. Sherer
Summary: Hepatitis B virus (HBV) capsid assembly is initially observed in the nucleus and later redistributed to the cytoplasm during nuclear envelope breakdown and cell division. This study provides dynamic evidence supporting the nucleus as an early-stage site of HBV capsid assembly and the cytoplasmic retention of capsids after cell division as a mechanism for nucleus-to-cytoplasm relocalization.
Article
Pharmacology & Pharmacy
Yang Liu, Baozeng Sun, Jingyu Pan, Yuancai Feng, Wei Ye, Jiahao Xu, Mingfu Lan, Hao Sun, Xiyang Zhang, Yuanjie Sun, Shuya Yang, Jingqi Shi, Fanglin Zhang, Linfeng Cheng, Dongbo Jiang, Kun Yang
Summary: An EBOV GP recombinant DNA vaccine targeting MHC class II compartment was developed to enhance immune responses against Ebola virus. Mice immunized with this vaccine showed significantly higher serum antibody titers and neutralizing activity compared to other groups, along with antigen-specific T cells demonstrating high affinity for dominant peptides. Preliminary safety observation indicated no histological alterations, suggesting promise for the DNA vaccine candidate in combating Ebola epidemic.
ANTIVIRAL RESEARCH
(2021)
Article
Biochemical Research Methods
Hao-Yang Liu, Zhi-Gang Wang, Shu-Lin Liu, Dai-Wen Pang
Summary: Single-virus tracking (SVT) allows real-time monitoring of individual viruses, enabling the exploration of their interactions with cellular structures. Quantum dot-based SVT (QSVT) overcomes the limitations of conventional fluorescent tags, providing more accurate and detailed information about viral infection in live cells.
Article
Virology
Shigeo Nagashima, Putu Prathiwi Primadharsini, Takashi Nishiyama, Masaharu Takahashi, Kazumoto Murata, Hiroaki Okamoto
Summary: This study developed a reporter hepatitis E virus (HEV) and demonstrated its efficient replication, genetic stability, and infectivity. The researchers also simplified the measurement of luciferase activity and used it to evaluate the effects of introducing small interfering RNAs and inhibitors on HEV release. These findings contribute to the understanding of the HEV life cycle and provide a more convenient screening platform for candidate drugs targeting the late stage of HEV infection.
JOURNAL OF VIROLOGY
(2023)
Article
Virology
Hiroyuki Mori, James P. Connell, Callie J. Donahue, RuthMabel Boytz, Yen Thi Kim Nguyen, Daisy W. Leung, Douglas J. LaCount, Robert A. Davey
Summary: This study identifies the actin-regulating protein CAPG as an important factor in the replication of Ebola virus. Knockdown of CAPG specifically inhibits viral infectivity and yield of infectious particles. Mechanistic analysis reveals the requirement of CAPG for virus production from infected cells, and the interaction between CAPG and the viral protein VP40.
Article
Biochemistry & Molecular Biology
Markus Hoffmann, Prerna Arora, Rudiger Gross, Alina Seidel, Bojan F. Hornich, Alexander S. Hahn, Nadine Kruger, Luise Graichen, Heike Hofmann-Winkler, Amy Kempf, Martin S. Winkler, Sebastian Schulz, Hans-Martin Jack, Bernd Jahrsdorfer, Hubert Schrezenmeier, Martin Muller, Alexander Kleger, Jan Munch, Stefan Pohlmann
Summary: The emerging SARS-CoV-2 variants may exhibit resistance to existing neutralizing antibodies and treatments, which could have significant implications for pandemic containment efforts.
Article
Chemistry, Multidisciplinary
Masafumi Minoshima, Taro Umeno, Kohei Kadooka, Margaux Roux, Namiko Yamada, Kazuya Kikuchi
Summary: To understand protein function in live cells, it is important to monitor protein dynamics and sense their environment in real-time. We developed a versatile chemical protein-labeling tool that efficiently forms stable carbamoylated complexes with beta-lactamase, allowing long-term visualization of labeled proteins in live cells. The use of an alpha-fluorinated carboxylate ester-based prodrug enables cell membrane permeability and stable labeling of intracellular proteins. Combining this labeling tool with a pH-activatable fluorescent probe enables visual monitoring of lysosomal protein translocation during autophagy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Immunology
Tim Birkle, G. C. Brown
Summary: In this novel mode of innate immunity, infected host cells induce phagocytes to phagocytose the infected cells, potentially reducing infection. The release of signals by infected host cells and the downregulation of inhibitory signals contribute to the phagocytosis process and potential destruction of pathogens within phagocytes, curbing infection and inducing an adaptive immune response.
INFECTION AND IMMUNITY
(2021)
Article
Biotechnology & Applied Microbiology
Takehiro Kanda, Madoka Sakai, Akiko Makino, Keizo Tomonaga
Summary: The study found that exogenous expression of Matrix protein (M) and Glycoprotein (G), which are constituents of the viral lipid envelope, significantly facilitates the formation and propagation of BoDV-1 infectious particles without affecting viral RNA synthesis. Additionally, simultaneous transfection of M and G expression plasmids with N, P, and L helper plasmids improves the rescue efficiency of recombinant BoDV-1. The findings suggest that BoDV-1 may suppress excess expression of M and G to reduce cytopathic effects, leading to persistent infection.
JOURNAL OF GENERAL VIROLOGY
(2022)
Article
Microbiology
Jinfeng Shao, Gunjan Arora, Javier Manzella-Lapeira, Joseph A. Brzostowski, Sanjay A. Desai
Summary: This study developed a new method called RISE, which enables continuous nondestructive tracking of antigen exposure on infected cells. Using RISE, researchers tracked the membrane insertion of a parasite antigen called CLAG3 in infected red blood cells. They found that CLAG3 insertion occurs at specific parasite stages and is required for the formation of a nutrient uptake channel. Furthermore, they defined constraints on protein insertion at the host cell membrane by varying the size and charge of the extracellular domain.
Article
Virology
Danielle Thompson, Christiana Victoria Cismaru, Jean-Sebastien Rougier, Martin Schwemmle, Gert Zimmer
Summary: The proton channel activity of bat influenza A virus (IAV) M2 proteins was found to be low, indicating that they are unable to fully protect acid-sensitive HA protein from conformational changes. The primary sequences of bat IAV M2 proteins also differ significantly from conventional IAV M2 proteins, suggesting possible differences in their biological function. This study reveals the importance of the proton channel activity of bat IAV M2 proteins in virus replication.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Christopher J. Benwell, James A. G. E. Taylor, Stephen D. Robinson
Summary: NRP2 plays a crucial role in promoting EC adhesion and migration, contributing to vascular formation. Further research is needed to fully understand its specific functions in angiogenesis.
Article
Multidisciplinary Sciences
Ja Yeon Kim Chu, Yu-Chen Chuang, Kuen-Nan Tsai, Jessica Pantuso, Yuji Ishida, Takeshi Saito, Jing-hsiung James Ou
Summary: This study reveals the association between autophagic membranes and HBV replication. The core protein is associated with phagophores, while the precore protein derivative is associated with autophagosomes. Autophagic membranes play different roles in the trafficking of core and precore proteins, as well as the assembly and release of HBV particles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Longlong Si, Quan Shen, Jing Li, Li Chen, Jinying Shen, Xue Xiao, Haiqing Bai, Tang Feng, Adam Yongxin Ye, Le Li, Chunhe Zhang, Zhen Li, Ping Wang, Crystal Yuri Oh, Atiq Nurani, Siwen Niu, Chengxin Zhang, Xiaoqiong Wei, Wanqiong Yuan, Hao Liao, Xiaojie Huang, Ning Wang, Wen-xia Tian, Hongwei Tian, Li Li, Xiaoheng Liu, Roberto Plebani
Summary: The study describes the generation of a live attenuated influenza A virus vaccine using PROTAC technology to degrade viral proteins via the host cell's ubiquitin-proteasome system. The vaccine showed highly attenuated and immune responses against both homologous and heterologous virus challenges in mouse and ferret models.
NATURE BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Alexandre Betourne, Marion Szelechowski, Anne Thouard, Erika Abrial, Arnaud Jean, Falek Zaidi, Charlotte Foret, Emilie M. Bonnaud, Caroline M. Charlier, Elsa Suberbielle, Cecile E. Malnou, Sylvie Granon, Claire Rampon, Daniel Gonzalez-Dunia
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Physics, Condensed Matter
A. Casanova, L. Bettamin, M-C Blatche, F. Mathieu, H. Martin, D. Gonzalez-Dunia, L. Nicu, G. Larrieu
JOURNAL OF PHYSICS-CONDENSED MATTER
(2018)
Review
Genetics & Heredity
E. Cecile Malnou, David Umlauf, Mailys Mouysset, Jerome Cavaille
FRONTIERS IN GENETICS
(2019)
Article
Genetics & Heredity
Alexia Martinez de Paz, Leila Khajavi, Helene Martin, Rafael Claveria-Gimeno, Susanne Tom Dieck, Manjinder S. Cheema, Jose Sanchez-Mut, Malgorzata M. Moksa, Annaick Carles, Nick Brodie, Taimoor Sheikh, Melissa E. Freeman, Evgeniy Petrotchenko, Christoph H. Borchers, Erin M. Schuman, Matthias Zytnicki, Adrian Velazquez-Campoy, Olga Abian, Martin Hirst, Manel Esteller, John B. Vincent, Cecile E. Malnou, Juan Ausio
EPIGENETICS & CHROMATIN
(2019)
Article
Physiology
Benjamin Portal, Sarah Delcourte, Renaud Rovera, Camille Lejards, Sebastien Bullich, Cecile E. Malnou, Nasser Haddjeri, Nicole Deglon, Bruno P. Guiard
Article
Oncology
Maude Rolland, Helene Martin, Mathilde Bergamelli, Yann Sellier, Bettina Bessieres, Jacqueline Aziza, Alexandra Benchoua, Marianne Leruez-Ville, Daniel Gonzalez-Dunia, Stephane Chavanas
Summary: Human cytomegalovirus (HCMV) infection leads to impaired migration of neural stem cells by increasing the expression of LIS1, resulting in developmental abnormalities and sequelae. Studies on infected NSCs suggest that PAFAH1B1 is a critical target of HCMV infection, shedding new light on the pathophysiological basis of congenital HCMV infection's neurological outcomes.
JOURNAL OF PATHOLOGY
(2021)
Letter
Immunology
Karine Bourgade, Anne Thouard, Florence Abravanel, Anne-Laure Hebral, Arnaud Del Bello, Alain Viguier, Daniel Gonzalez-Dunia, Nassim Kamar
TRANSPLANT INFECTIOUS DISEASE
(2021)
Article
Cell Biology
Mathilde Bergamelli, Helene Martin, Melinda Benard, Jerome Ausseil, Jean-Michel Mansuy, Ilse Hurbain, Mailys Mouysset, Marion Groussolles, Geraldine Cartron, Yann Tanguy le Gac, Nathalie Moinard, Elsa Suberbielle, Jacques Izopet, Charlotte Tscherning, Graca Raposo, Daniel Gonzalez-Dunia, Gisela D'Angelo, Cecile E. Malnou
Summary: Extracellular vesicles (EVs) play important roles during pregnancy, serving as both potential biomarkers and mediators in communication between the placenta and maternal/fetal sides. Infection can alter the expression levels of surface markers on EVs, while still maintaining their secretion and integrity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Florent Henri Marty, Luca Bettamin, Anne Thouard, Karine Bourgade, Sophie Allart, Guilhem Larrieu, Cecile Evelyne Malnou, Daniel Gonzalez-Dunia, Elsa Suberbielle
Summary: In this study, it was found that Borna disease virus infection or expression of viral nucleoprotein and phosphoprotein increased DNA double-strand break (DSB) levels in neurons. Inducing DSB promoted the recruitment of viral replication centers colocalized with DSB and enhanced viral RNA replication. BoDV-1 persistence decreased neuronal activity and response to stimulation by reducing the surface expression of glutamate receptors. These findings suggest a novel mechanistic cross talk between RNA virus persistence and neuronal function through the control of DSB levels.
Article
Virology
Helene Martin, Jonathan Barthelemy, Yamileth Chin, Mathilde Bergamelli, Nathalie Moinard, Geraldine Cartron, Yann Tanguy Le Gac, Cecile E. Malnou, Yannick Simonin
Summary: Usutu virus (USUV) is a neurotropic mosquito-borne flavivirus that has rapidly spread in Europe. Although mostly asymptomatic or causing mild symptoms, it can lead to neurological disorders in humans. The study demonstrates that USUV can replicate in human placental cells and tissues and suggests its potential transmission during gestation in mice.
Article
Virology
Mathilde Bergamelli, Helene Martin, Yann Aubert, Jean-Michel Mansuy, Marlene Marcellin, Odile Burlet-Schiltz, Ilse Hurbain, Graca Raposo, Jacques Izopet, Thierry Fournier, Alexandra Benchoua, Melinda Benard, Marion Groussolles, Geraldine Cartron, Yann Tanguy Le Gac, Nathalie Moinard, Gisela D'Angelo, Cecile E. Malnou
Summary: This study reveals that human cytomegalovirus (hCMV) infection increases the production of placental small extracellular vesicles (sEVs) and modifies their protein content, potentially favoring viral dissemination. These sEVs also potentiate infection in cells of fetal origin.
Review
Biochemistry & Molecular Biology
Charlene Martin, Mathilde Bergamelli, Cecile E. Malnou, Gisela 'Angelo
Summary: This review examines the role of placental extracellular vesicles (EVs) during pregnancy. Placental EVs have diverse functions and play a role in various aspects of normal pregnancy, as well as in certain pathological pregnancies. The deregulation of placental EVs in terms of abundance and composition during pathological pregnancies suggests their potential as non-invasive biomarkers for gestational diseases.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Article
Multidisciplinary Sciences
Cecile A. Ferre, Anne Thouard, Alexandre Betourne, Anne-Louise Le Dorze, Pascale Belenguer, Marie-Christine Miquel, Jean-Michel Peyrin, Daniel Gonzalez-Dunia, Marion Szelechowski
Summary: The study demonstrated that Mortalin expression levels influence neuronal fate, with over-expression providing protection against rotenone-induced damage and down-regulation leading to mitochondrial fragmentation and axonal damage. Additionally, Mortalin levels were shown to modulate mitochondrial morphology through DRP1 phosphorylation, further emphasizing the crucial role of mitochondrial dynamics in neuronal fate in degenerative diseases.
SCIENTIFIC REPORTS
(2021)