Article
Multidisciplinary Sciences
Beatriz Alvarez-Rodriguez, Javier Buceta, Ron Geller
Summary: Despite limited understanding, a comprehensive analysis reveals significant variation in the neutralization sites targeted by polyclonal antibody responses to non-enveloped viruses in individual humans. This knowledge helps predict viral escape and identify key structural and evolutionary parameters. A minimum of two mutations is required for significant escape from multiple sera.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Jingzhe Wang, Tonggong Liu, Siyao Gu, Hui-hui Yang, Weidong Xie, Cheng Gao, Dayong Gu
Summary: This study introduces a novel cardiomyocyte sponge to trap and neutralize the virus causing viral myocarditis. The sponge effectively inhibits virus infection by maintaining cellular morphology. The most significant implication of this study is the development of a universal antivirus infection strategy for treating known and unknown virus outbreaks.
Article
Biochemistry & Molecular Biology
Janik Boehnke, Sandra Pinkert, Maria Schmidt, Hans Binder, Nicole Christin Bilz, Matthias Jung, Uta Reibetanz, Antje Beling, Dan Rujescu, Claudia Claus
Summary: This study discusses the potential association of enteroviruses with pregnancy complications, including miscarriages, and characterizes the infection of human induced pluripotent stem cells and their derived cells by coxsackievirus B3. The research shows that CVB3 infection reduces pluripotency gene expression and mesodermal cells are particularly vulnerable to infection. This highlights the importance of considering enteroviruses in pregnancy screening programs and suggests that iPSCs could be a valuable tool for studying viral infections in specific target organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Virology
Anja Geisler, Ahmet Hazini, Lisanne Heimann, Jens Kurreck, Henry Fechner
Summary: CVB3 as an oncolytic virus shows broad anti-tumor activity and enhances treatment efficacy by inducing an immune response against tumors. The toxicity in healthy tissues varies and can be reduced through genetic engineering. However, further efforts are needed to develop CVB3 as a therapeutic agent for cancer treatment in clinical practice.
Article
Oncology
Amirhossein Bahreyni, Huitao Liu, Yasir Mohamud, Yuan Chao Xue, Jingchun Zhang, Honglin Luo
Summary: The study found that miR-CVB3-1.1 can safely infect and lyse breast cancer cells, significantly inhibiting tumor growth and metastasis. Mice treated with miR-CVB3-1.1 showed reduced toxicity and prolonged survival.
Article
Oncology
Huitao Liu, Amirhossein Bahreyni, Yasir Mohamud, Yuan Chao Xue, William W. G. Jia, Honglin Luo
Summary: Inserting target sequences of tumor-suppressive and/or organ-selective microRNAs into the viral genome of coxsackievirus B3 (CVB3) can eliminate viral pathogenesis without impacting its oncolytic activity. To improve stability, the CVB3 was re-engineered by replacing viral genome regions or inserting target sequences in specific regions. These modified CVB3s demonstrated improved stability and retained their ability to suppress lung tumor growth in mice.
MOLECULAR THERAPY-ONCOLYTICS
(2022)
Article
Virology
Martin Voss, Vera Braun, Clara Bredow, Peter-Michael Kloetzel, Antje Beling
Summary: Infection with coxsackievirus B3 results in impaired protein homeostasis and accumulation of ubiquitinylated proteins, attributed to viral proteins 2B and 3A. During the exponential replication phase, oxidative damage of membrane proteins is reduced due to the recruitment of glutathione. The proteasome is shown to play a role in processing viral precursor proteins during infection.
Article
Immunology
Julia Mamana, Gabrielle M. Humber, Eric R. Espinal, Soojung Seo, Nadine Vollmuth, Jon Sin, Brandon J. Kim
Summary: Coxsackievirus B3 (CVB3) is a common human pathogen that can cause fatal aseptic meningo-encephalitis, particularly in young children. The mechanisms by which CVB3 gains access to the brain and the virus-host interactions at the blood-brain barrier (BBB) are poorly understood. This study utilized human induced-pluripotent stem cell-derived brain-like endothelial cells (iBECs) to investigate the effects of CVB3 infection on BBB function and overall survival. The results showed that CVB3 infection of iBECs resulted in high extracellular virus release and disruption of transendothelial electrical resistance (TEER) at later infection stages, suggesting potential mechanisms for viral entry and prolonged shedding.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Oncology
Miyako Sagara, Shohei Miyamoto, Shun Itoh, Yasushi Soda, Kenzaburo Tani
Summary: The study demonstrated that CVB3 can effectively kill breast cancer cells, especially TNBC cells. CVB3-HP showed strong oncolytic activity and a low rate of adverse events in a mouse model, indicating its potential as a therapeutic candidate for breast cancer.
ANTICANCER RESEARCH
(2021)
Article
Virology
Ahmet Hazini, Babette Dieringer, Karin Klingel, Markian Pryshliak, Anja Geisler, Dennis Kobelt, Ole Daberkow, Jens Kurreck, Sophie van Linthout, Henry Fechner
Summary: The oncolytic virus PD-H demonstrated strong inhibition of tumor growth and prolonged survival in colorectal cancer models, although virus-induced systemic infection was observed in some cases. Additionally, engineering the virus with specific microRNA target sites showed potential for reducing side effects and enhancing therapeutic effects.
Article
Biotechnology & Applied Microbiology
Ahmet Hazini, Babette Dieringer, Markian Pryshliak, Klaus-Peter Knoch, Lisanne Heimann, Beatrice Tolksdorf, Kathleen Pappritz, Muhammad El-Shafeey, Michele Solimena, Antje Beling, Jens Kurreck, Karin Klingel, Henry Fechner
Summary: Insertion of target sites complementary to miR-375 and miR-1 into the oncolytic virus genome can prevent pancreas and heart toxicity, allowing selective attack on colorectal carcinoma cells. The engineered viruses showed reduced replication in cell lines expressing the corresponding microRNAs, leading to improved safety and therapeutic efficiency in mouse models.
HUMAN GENE THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Jennifer M. Podgorski, Krista Freeman, Sophia Gosselin, Alexis Huet, James F. Conway, Mary Bird, John Grecco, Shreya Patel, Deborah Jacobs-Sera, Graham Hatfull, Johann Peter Gogarten, Janne Ravantti, Simon J. White
Summary: Many double-stranded DNA viruses, including tailed bacteriophages and herpesviruses, use the HK97-fold in their major capsid protein. The evolution of capsid structural stability within the actinobacteriophages has been investigated using cryo-EM structure determination, structural comparisons, phylogenetic analyses, and AlphaFold predictions. The actinobacteriophage major capsid proteins can be classified into 15 groups based on their HK97-fold.
Review
Biochemistry & Molecular Biology
Miles Graham, Peijun Zhang
Summary: Developments in cryo-electron microscopy have been closely linked to the study of viruses. Cryo-electron tomography is rapidly maturing as a technology and advancing structural virology. This review provides an overview of the use of cryo-ET to study viral infection biology, including the workflows and strategies used. It discusses studies on purified viruses and virus-like particles, as well as characterizing host-virus interactions and membrane fusion events. The importance of in situ cellular imaging and the need for validation of high-resolution findings are also highlighted.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Javier O. Cifuente, Natalia Comino, Julene Madariaga-Marcos, Sonia Lopez-Fernandez, Mikel Garcia-Alija, Jon Agirre, David Albesa-Jove, Marcelo E. Guerin
Article
Multidisciplinary Sciences
David Albesa-Jove, Zuzana Svetlikova, Montse Tersa, Enea Sancho-Vaello, Ana Carreras-Gonzalez, Pascal Bonnet, Pedro Arrasate, Ander Eguskiza, Shiva K. Angala, Javier O. Cifuente, Jana Kordulakova, Mary Jackson, Katarina Mikusova, Marcelo E. Guerin
NATURE COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Natalia Comino, Javier O. Cifuente, Alberto Marina, Ane Orrantia, Ander Eguskiza, Marcelo E. Guerin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Virology
Suriyasri Subramanian, Lindsey J. Organtini, Alec Grossman, Philip P. Domeier, Javier O. Cifuente, Alexander M. Makhov, James F. Conway, Anthony D'Abramo, Susan F. Cotmore, Peter Tattersall, Susan Hafenstein
Article
Virology
Kristin L. Shingler, Javier O. Cifuente, Robert E. Ashley, Alexander M. Makhov, James F. Conway, Susan Hafenstein
JOURNAL OF VIROLOGY
(2015)
Article
Biochemistry & Molecular Biology
Nicole Paczia, Julia Becker-Kettern, Jean-Francois Conrotte, Javier O. Cifuente, Marcelo E. Guerin, Carole L. Linster
Review
Biochemistry & Molecular Biology
Rainer Kalscheuer, Ainhoa Palacios, Itxaso Anso, Javier Cifuente, Juan Anguita, William R. Jacobs, Marcelo E. Guerin, Rafael Prados-Rosales
BIOCHEMICAL JOURNAL
(2019)
Review
Biochemistry & Molecular Biology
Javier O. Cifuente, Natalia Comino, Beatriz Trastoy, Cecilia D'Angelo, Marcelo E. Guerin
BIOCHEMICAL JOURNAL
(2019)
Article
Multidisciplinary Sciences
Hyunwook Lee, Heather M. Callaway, Javier O. Cifuente, Carol M. Bator, Colin R. Parrish, Susan L. Hafenstein
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Review
Immunology
Javier Orlando Cifuente, Gonzalo Moratorio
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Diego E. Sastre, Luis G. M. Basso, Beatriz Trastoy, Javier O. Cifuente, Xabier Contreras, Frederico Gueiros-Filho, Diego de Mendoza, Marcos V. A. S. Navarro, Marcelo E. Guerin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Beatriz Trastoy, Jonathan J. Du, Erik H. Klontz, Chao Li, Javier O. Cifuente, Lai-Xi Wang, Eric J. Sundberg, Marcelo E. Guerin
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Ane Rodrigo-Unzueta, Mattia Ghirardello, Saioa Urresti, Ignacio Delso, David Giganti, Itxaso Anso, Beatriz Trastoy, Natalia Comino, Montse Tersa, Cecilia D'Angelo, Javier O. Cifuente, Alberto Marina, Jobst Liebau, Lena Maler, Alexandre Chenal, David Albesa-Jove, Pedro Merino, Marcelo E. Guerin
Article
Multidisciplinary Sciences
Itxaso Anso, Andreas Naegeli, Javier O. Cifuente, Ane Orrantia, Erica Andersson, Olatz Zenarruzabeitia, Alicia Moraleda-Montoya, Mikel Garcia-Alija, Francisco Corzana, Rafael A. Del Orbe, Francisco Borrego, Beatriz Trastoy, Jonathan Sjogren, Marcelo E. Guerin
Summary: Red blood cell antigens are critical in blood transfusion. Researchers have discovered a bacterial enzyme called FucOB that can convert universal O type blood into the rare Bombay type blood. This finding has the potential to facilitate transfusion in recipients with the Bombay phenotype.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Javier O. Cifuente, Natalia Comino, Cecilia D'Angelo, Alberto Marina, David Gil-Carton, David Albesa-Jove, Marcelo E. Guerin
CURRENT RESEARCH IN STRUCTURAL BIOLOGY
(2020)