Article
Engineering, Environmental
Shu Xiao, Cong Cao, Tinghong Ming, Yuting Cao, Zhenzhong Yu, Ning Gan
Summary: To solve the challenge of simultaneous detection of live and dead bacteria in food safety, researchers developed an all-in-one biosensor using a phage-apoferritin@CuO2 probe on an antimicrobial peptide (AMP)/MXenes-modified detection platform. The biosensor can simultaneously measure live and dead bacteria of Escherichia coli O157:H7 through bioluminescent, colorimetric, and electrochemical signals. The biosensor was successfully used for on-site measurement of live and dead E. coli O157:H7 in food samples with a detection limit of 30 CFU/mL for live bacteria and 6 CFU/mL for total bacteria within 50 min. This work presents a novel pathway for rapid and simultaneous quantification of both live and dead bacteria.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Food Science & Technology
Lais Silva Batalha, Marco Tulio Pardini Gontijo, Alvaro Vianna Novaes de Carvalho Teixeira, Delaine Meireles Gouvea Boggione, Maryoris Elisa Soto Lopez, Monique Renon Eller, Regina Celia Santos Mendonca
Summary: The study showed that bacteriophages can be effectively encapsulated using different hydrocolloids via extrusion, with the encapsulating solutions displaying non-Newtonian pseudoplastic behavior. The concentration of hydrocolloids did not affect the morphology of the beads, except for alginate-chitosan solutions which exhibited unique flow properties. The encapsulated bacteriophages remained viable and showed homogenous distribution within the beads, highlighting the potential of this encapsulation system for bioactive compound entrapment.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Microbiology
Su-Hyeon Kim, Damilare Emmanuel Adeyemi, Mi-Kyung Park
Summary: KFS-EC3 is a polyvalent and lytic phage that can efficiently infect various bacteria, making it suitable for pathogen control in foods.
Article
Biochemistry & Molecular Biology
Mohamad Ismail Sultan-Alolama, Amr Amin, Khaled A. El-Tarabily, Ranjit Vijayan
Summary: This study reports the isolation of a phage, UAE_MI-01, from bird feces for the first time, indicating birds as a potential source of phages. The phage was found to be lytic to Verotoxin-producing Escherichia coli O157:H7 and showed stability under varying conditions. The presence of the phage suggests the presence of the host bacteria in wild birds, making it important to investigate other bird species for this pathogenic bacterium.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Tao Yang, Zhenghui Wang, Yang Song, Xinyan Yang, Sihan Chen, Shiqian Fu, Xue Qin, Wei Zhang, Chaoxin Man, Yujun Jiang
Summary: A smartphone-based colorimetric aptasensor was developed for the detection of E. coli O157:H7 in milk, showing good reproducibility with no cross-reaction for other bacteria. The sensor could detect a concentration of 8.43 x 10^3 cfu/mL of E. coli O157:H7 in pure culture and 5.24 x 10^2 cfu/mL in artificially contaminated milk after 1 hour of incubation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Haiying Cui, Xiaojing Yang, Changzhu Li, Yong Ye, Xiaochen Chen, Lin Lin
Summary: A strategy to simultaneously remove Escherichia coli O157 and its biofilms was successfully developed using composite nanofiber films. The films, synthesized by encapsulating E. coli O157 phages and D-phenylalanine into sodium alginate/polyethylene oxide nanofibers, showed excellent antibacterial activity against both free E. coli O157 and biofilms. The addition of D-phenylalanine significantly enhanced the inhibitory activity against E. coli O157 proliferation and biofilm formation during short-term storage of beef and fruits and vegetables.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Zhihua Li, Xue Zhang, Haishan Qi, Xiaowei Huang, Jiyong Shi, Xiaobo Zou
Summary: A renewable electrochemical biosensor based on mussel-inspired adhesive protein was developed for the detection of Escherichia coli O157:H7. The biosensor showed excellent repeatability and sensitivity, with a wide linear range and a low detection limit. It demonstrated good performance in detecting E. coli O157:H7 in seafood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Veterinary Sciences
Delphine Bibbal, Philippe Ruiz, Panagiotis Sapountzis, Christine Mazuy-Cruchaudet, Estelle Loukiadis, Frederic Auvray, Evelyne Forano, Hubert Brugere
Summary: This study aimed to identify farms with persistent circulation of EHEC O157:H7 in cattle. The results showed that persistent circulation may be due to the presence of a few predominant strains or repeated exposure of cattle to various strains. In addition, differences were found in the fecal microbial communities of shedders and non-shedders, with different bacterial indicators identified.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Yan D. Niu, Hui Liu, Hechao Du, Ruiqiang Meng, El Sayed Mahmoud, Guihua Wang, Tim A. McAllister, Kim Stanford
Summary: In this study, the efficacy of different bacteriophages against E. coli O157 was evaluated, revealing that the combination of phages did not always follow the individual phage activity. Factors such as temperature, exposure time, and phage strains influenced the interactions among phages, with some combinations showing facilitation while others demonstrated antagonistic effects. The development of phage cocktails should consider confirming lack of antagonism and preferably facilitation or synergistic effects among constituent phages.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Kai Dong, Yuwan Li, Sijia Luo, Fan Zhang, Hanxu Pan, Liang Zhao, Yongtao Wang, Xiaojun Liao
Summary: The study analyzed the sterilizing effects of different HHP-boosting processes on Escherichia coli O157:H7, finding that linear and fast boosting had the most significant bactericidal effects. The proteins interacting with GroEL under different boost modes and rates were involved in 42 metabolic pathways, particularly ribosome, ABC transporter, oxidative phosphorylation, sulfate assimilation, and cysteine biosynthesis. Key interacting proteins NuoB, NuoF, CysD, ZnuC, and RplX were identified and their interactions with GroEL were verified in vivo.
Article
Veterinary Sciences
Hanjie Yin, Jing Li, Haosheng Huang, Yuxin Wang, Xinjie Qian, Jianluan Ren, Feng Xue, Jianjun Dai, Fang Tang
Summary: This study focused on microencapsulating E. coli O157:H7 phages to improve their stability in the gastrointestinal tract. The results showed that microencapsulated phages had increased resistance to simulated gastric fluid and bile salts, and more efficient release into simulated intestinal fluid, leading to a better therapeutic effect in rats infected with E. coli O157:H7 compared to free phages. Additionally, microencapsulated phages were more stable during storage, highlighting the essential role of phage microencapsulation in phage therapy.
VETERINARY RESEARCH
(2021)
Article
Immunology
Juliana Gonzalez, Jimena Soledad Cadona, Claudio Marcelo Zotta, Silvina Lavayen, Roberto Vidal, Nora Lia Padola, Andrea Mariel Sanso, Ana Victoria Bustamante
Summary: This study aimed to compare the genetic diversity of VTEC O157:H7 strains isolated from human disease cases in Argentina and Chile. The results showed that these strains mainly belonged to lineage I/II, associated with hypervirulent strains, and were categorized into the phylogroup E. Additionally, genetic diversity was observed among Argentinean and Chilean strains, particularly in relation to putative virulence determinants and nle profiles.
MICROBES AND INFECTION
(2022)
Article
Veterinary Sciences
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study estimated the prevalence and antimicrobial resistance of Escherichia coli O157:H7 in raw beef sold in butcher shops in Addis Ababa, Ethiopia. Out of the 384 samples examined, 3.64% carried E. coli O157:H7, with 57.14% of the isolates showing resistance to three or more antimicrobial agents. Thus, stricter monitoring of antimicrobial use and further studies on contamination sources and risk mitigation strategies are needed.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Analytical
Manman Du, Jinbiao Ma, Zhiwei Zhang, Guangzu Wu, Jianguo Wu, Hao Wang, Xinwu Xie, Can Wang
Summary: This study developed a rGO-FET biosensor for the rapid and sensitive detection of pathogenic microorganisms. The biosensor demonstrated good specificity, a wide linear range, and the ability to directly detect real samples without pretreatment.
ANALYTICA CHIMICA ACTA
(2023)
Correction
Virology
Stuart G. Siddell, Peter J. Walker, Elliot J. Lefkowitz, Arcady R. Mushegian, Bas E. Dutilh, Balazs Harrach, Robert L. Harrison, Sandra Junglen, Nick J. Knowles, Andrew M. Kropinski, Mart Krupovic, Jens H. Kuhn, Max L. Nibert, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Arvind Varsani, Francisco Murilo Zerbini, Andrew J. Davison
ARCHIVES OF VIROLOGY
(2020)
Article
Virology
Stuart G. Siddell, Peter J. Walker, Elliot J. Lefkowitz, Arcady R. Mushegian, Bas E. Dutilh, Balazs Harrach, Robert L. Harrison, Sandra Junglen, Nick J. Knowles, Andrew M. Kropinski, Mart Krupovic, Jens H. Kuhn, Max L. Nibert, Luisa Rubino, Sead Sabanadzovic, Peter Simmonds, Arvind Varsani, Francisco Murilo Zerbini, Andrew J. Davison
ARCHIVES OF VIROLOGY
(2020)
Article
Genetics & Heredity
Jinwei Suo, Heng Zhang, Qi Zhao, Nan Zhang, Yongxue Zhang, Ying Li, Baohua Song, Juanjuan Yu, Jianguo Cao, Tai Wang, Ji Luo, Lihai Guo, Jun Ma, Xumin Zhang, Yimin She, Lianwei Peng, Weimin Ma, Siyi Guo, Yuchen Miao, Sixue Chen, Zhi Qin, Shaojun Dai
GENOMICS PROTEOMICS & BIOINFORMATICS
(2020)
Article
Chemistry, Analytical
Yi-Min She, Roger Y. Tam, Xuguang Li, Michael Rosu-Myles, Simon Sauve
ANALYTICAL CHEMISTRY
(2020)
Article
Infectious Diseases
Johannes Wittmann, Dann Turner, Andrew D. Millard, Padmanabhan Mahadevan, Andrew M. Kropinski, Evelien M. Adriaenssens
Article
Multidisciplinary Sciences
Zhijuan Chen, Vanessa Lancon-Verdier, Christine Le Signor, Yi-Min She, Yun Kang, Jerome Verdier
Summary: This study used the genetic diversity in Medicago truncatula to identify genes/loci regulating seed traits through a genome-wide association study, revealing a strong positive correlation between seed size and protein content, as well as multiple QTNs associated with different seed phenotypes. The findings represent valuable resources for improving legume grain production in species such as pea, common bean, and soybean.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Dann Turner, Andrew M. Kropinski, Evelien M. Adriaenssens
Summary: Bacteriophage taxonomy has been undergoing significant changes in recent years, with the abolition of certain classification units and the establishment of guidelines for delineating species, genus, subfamily, and family-level ranks in tailed phage taxonomy.
Article
Plant Sciences
Ryan Kilburn, Suzanne A. Gerdis, Yi-Min She, Wayne A. Snedden, William C. Plaxton
Summary: Phosphoenolpyruvate carboxylase (PEPC) plays a crucial role in plant metabolism. In castor oil seeds, a bacterial-type PEPC forms a complex with Ricinus communis Ca2+-dependent protein kinase-1 (RcCDPK1) which regulates its activity through phosphorylation. The autophosphorylation of RcCDPK1 facilitates its interaction with PEPC and regulates its transphosphorylation activity.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yi-Min She, Kody Klupt, Grayson Hatfield, Zongchao Jia, Roger Y. Tam
Summary: Mannosidases are crucial enzymes that play diverse roles in cellular processes. We discovered unexpected β1-4-galactosidase activity in the α1-6-mannosidase, providing insights into its dual glycosidase specificity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Jianjun Zhang, Yang Xia, Dinghe Wang, Yamin Du, Yongwu Chen, Congcong Zhang, Juan Mao, Muyang Wang, Yi-Min She, Xinxiang Peng, Li Liu, Josef Voglmeir, Zuhua He, Linchuan Liu, Jianming Li
Summary: This study demonstrates that in rescuing bri1-5 protein degradation, AtEDEM1 plays a predominant role over AtEDEM2, while a mutation in ALG3 eliminates the inhibitory effect of irb1-1 on bri1-5 ERAD.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Virology
Janet T. Lin, Sarah Kirst, Stevan Cucic, Alexandra Klem, Yi-Min She, Andrew M. Kropinski, Hany Anany
Summary: A novel bacteriophage with high specificity and a long latent period was isolated from EHEC-enriched sewage, expanding our understanding of bacteriophage diversity.
Article
Multidisciplinary Sciences
Yi-Min She, Shaojun Dai, Roger Y. Tam
Summary: Glycosylation is an important attribute for assessing the manufacturing quality of mAbs. We developed a highly sensitive LC MS/MS method for comprehensive characterization of low-abundance non-human glycans and their structural isomers. Our findings reveal the structural diversity of non-human glycans and the sulfation of alpha-galactosylated glycans, providing an analytical method and candidate structures for quality control of therapeutic mAb products.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Thomas S. Postler, Luisa Rubino, Evelien M. Adriaenssens, Bas E. Dutilh, Balazs Harrach, Sandra Junglen, Andrew M. Kropinski, Mart Krupovic, Jiro Wada, Anya Crane, Jens H. Kuhn, Arcady Mushegian, Janis Rumnieks, Sead Sabanadzovic, Peter Simmonds, Arvind Varsani, F. Murilo Zerbini, Julie Callanan, Lorraine A. Draper, Colin Hill, Stephen R. Stockdale
Summary: The International Committee on Taxonomy of Viruses has adopted a binomial naming format for virus species, which is comparable with other biological taxonomies. Latinization of these names is optional, but it provides the advantage of cultural neutrality and compatibility with other taxonomies.
JOURNAL OF GENERAL VIROLOGY
(2022)
Article
Microbiology
Dann Turner, Evelien M. Adriaenssens, Igor Tolstoy, Andrew M. Kropinski
Summary: Before submitting a bacteriophage genome sequence to the International Nucleotide Sequence Database Collaboration or writing a publication, it is important to address 14 questions to ensure an accurate and comprehensive annotation of the genome.
PHAGE-THERAPY APPLICATIONS AND RESEARCH
(2021)
Article
Virology
Cristina Moraru, Arvind Varsani, Andrew M. Kropinski
