Article
Virology
Pia Banse, Rebecca Moeller, Janina Bruening, Lisa Lasswitz, Sina Kahl, Abdul G. Khan, Joseph Marcotrigiano, Thomas Pietschmann, Gisa Gerold
Article
Gastroenterology & Hepatology
Tanvi Khera, Patrick Behrendt, Dorothea Bankwitz, Richard J. P. Brown, Daniel Todt, Mandy Doepke, Abdul Ghafoor Khan, Kai Schulze, John Law, Michael Logan, Darren Hockman, Jason Alexander Ji-Xhin Wong, Leona Dold, Victor Gonzalez-Motos, Ulrich Spengler, Abel Viejo-Borbolla, Luisa J. Stroeh, Thomas Krey, Alexander W. Tarr, Eike Steinmann, Michael P. Manns, Florian Klein, Carlos A. Guzman, Joseph Marcotrigiano, Michael Houghton, Thomas Pietschmann
JOURNAL OF HEPATOLOGY
(2019)
Article
Multidisciplinary Sciences
Vadim Kotov, Kim Bartels, Katharina Veith, Inokentijs Josts, Udaya K. Tiruttani Subhramanyam, Christian Guenther, Jorg Labahn, Thomas C. Marlovits, Isabel Moraes, Henning Tidow, Christian Loew, Maria M. Garcia-Alai
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Emily Z. Guo, Daniel C. Desrosiers, Jan Zalesak, James Tolchard, Melanie Berbon, Birgit Habenstein, Thomas Marlovits, Antoine Loquet, Jorge E. Galan
Article
Multidisciplinary Sciences
Jiri Wald, Marion Pasin, Martina Richter, Christin Walther, Neann Mathai, Johannes Kirchmair, Vadim A. Makarov, Nikolaus Goessweiner-Mohr, Thomas C. Marlovits, Irene Zanella, Antonio Real-Hohn, Nuria Verdaguer, Dieter Blaas, Michaela Schmidtke
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biology
Antoine Chabloz, Jonas V. Schaefer, Ivona Kozieradzki, Shane J. F. Cronin, Daniel Strebinger, Francesca Macaluso, Jiri Wald, Terence H. Rabbitts, Andreas Plueckthun, Thomas C. Marlovits, Josef M. Penninger
COMMUNICATIONS BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Nikos Pinotsis, Karolina Zielinska, Mrigya Babuta, Joan L. Arolas, Julius Kostan, Muhammad Bashir Khan, Claudia Schreiner, Anita Salmazo, Luciano Ciccarelli, Martin Puchinger, Eirini A. Gkougkoulia, Euripedes de Almeida Ribeiro, Thomas C. Marlovits, Alok Bhattacharya, Kristina Djinovic-Carugo
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Vadim Kotov, Georg Mlynek, Oliver Vesper, Marina Pletzer, Jiri Wald, Celso M. Teixeira-Duarte, Herve Celia, Maria Garcia-Alai, Stephan Nussberger, Susan K. Buchanan, Joao H. Morais-Cabral, Christian Loew, Kristina Djinovic-Carugo, Thomas C. Marlovits
Summary: Protein stability is crucial for structural and biochemical research, and the NanoDSF assay offers a miniaturized approach for assessing protein stability by measuring intrinsic tryptophan fluorescence. By simplifying protein unfolding description, this method can provide improved stability measures, such as the apparent Gibbs free energy of unfolding, for ranking results. The assay is compatible with diverse protein samples, making it a valuable tool for various applications including protein complex and membrane protein analysis.
Article
Multidisciplinary Sciences
Sean Miletic, Dirk Fahrenkamp, Nikolaus Goessweiner-Mohr, Jiri Wald, Maurice Pantel, Oliver Vesper, Vadim Kotov, Thomas C. Marlovits
Summary: The study suggests that pathogenic bacteria can inject effector proteins directly into host cell cytoplasm through virulent type III secretion systems or injectisomes. Structures of a needle complex engaged with the effector protein reveal the complete secretion channel and provide insights into the mechanism of substrate translocation through T3SSs.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Catalin M. Bunduc, Dirk Fahrenkamp, Jiri Wald, Roy Ummels, Wilbert Bitter, Edith N. G. Houben, Thomas C. Marlovits
Summary: The T7SS inner-membrane complex of Mycobacterium tuberculosis is unique in structure and essential for virulence, with the protease MycP(5) playing a crucial role in complex integrity. This study reveals a previously undescribed mechanism of protein transport and provides a potential target for drug development against this major human pathogen.
Article
Multidisciplinary Sciences
Maxime Killer, Jiri Wald, Joanna Pieprzyk, Thomas C. Marlovits, Christian Loew
Summary: The uptake of peptides in mammals is crucial for nutrition and inflammatory diseases, which is mediated by transporters in solute carrier family 15. The structures of human PepT1 and PepT2 captured in different states provide insights into substrate recognition and conformational transitions during transport, supporting future drug design efforts to enhance bioavailability in the human body.
Article
Biochemistry & Molecular Biology
Julien R. C. Bergeron, Thomas C. Marlovits
Summary: The structure of double-membrane-spanning protein complexes, such as the T3SS, has long been a challenge in structural biology. However, the emergence of cryo-electron microscopy has transformed our understanding of this complex, allowing us to determine high-resolution structures and gain insights into its mechanism of substrate transport. This review summarizes the recent structures of T3SS components determined by cryo-EM, discusses the remaining regions to be characterized, and highlights the recent structural insights into the mechanism of effector transport. It also emphasizes the challenges that still need to be addressed.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jiri Wald, Dirk Fahrenkamp, Nikolaus Goessweiner-Mohr, Wolfgang Lugmayr, Luciano Ciccarelli, Oliver Vesper, Thomas C. Marlovits
Summary: This study provides insights into the structure and mechanism of the ATP-hydrolysing RuvAB complex during the assembly and processing of the Holliday junction. The findings reveal the spatiotemporal relationship between ATP hydrolysis, nucleotide exchange, and conformational changes, and elucidate the mechanistic basis for DNA recombination.
Article
Biochemical Research Methods
Wolfgang Lugmayr, Vadim Kotov, Nikolaus Goessweiner-Mohr, Jiri Wald, Frank DiMaio, Thomas C. Marlovits
Summary: StarMap is an easy-to-use interface that improves the accuracy of macromolecular structure models by combining multiple methods, including Monte Carlo sampling, local density-guided optimization, Rosetta-based all-atom refinement, and real-space B-factor calculations. It has a graphical user interface that does not require coding knowledge and can be operated through simple clicks.
Article
Biochemistry & Molecular Biology
Erik Genthe, Sean Miletic, Indira Tekkali, Rory Hennell James, Thomas C. Marlovits, Philipp Heuser
Summary: In this paper, a deep learning framework called PickYOLO is introduced to address the problem of particle localization in cryoET analysis. By training the network, PickYOLO can quickly and accurately detect a large number of particles, significantly reducing the time and manual effort needed for data analysis, thus aiding in high-resolution cryoET structure determination.
JOURNAL OF STRUCTURAL BIOLOGY
(2023)