Article
Cell Biology
Mariana Castro Dias, Adolfo Odriozola Quesada, Sasha Soldati, Fabio Bosch, Isabelle Gruber, Tobias Hildbrand, Derya Sonmez, Tejas Khire, Guillaume Witz, James L. McGrath, Joerg Piontek, Masuo Kondoh, Urban Deutsch, Benoit Zuber, Britta Engelhardt
Summary: The study suggests that tricellular junctions of the BBB serve as novel sites for T cell diapedesis, different from the previously believed cellular junctions. By manipulating the expression of tricellular junctional proteins or targeting chemokines, the ability of T cells to cross the BBB can be enhanced or suppressed.
JOURNAL OF CELL SCIENCE
(2021)
Review
Biotechnology & Applied Microbiology
Janne Verreycken, Paulien Baeten, Bieke Broux
Summary: Multiple sclerosis (MS) is an autoimmune disorder characterized by demyelination and neurodegeneration in the central nervous system. Regulatory T cells (Tregs), which have the ability to suppress immune reactions and regenerate, may have therapeutic potential for MS. However, Tregs in people with MS are functionally impaired. To exert their anti-inflammatory and regenerative effects in the brain, Tregs must first migrate across the blood-brain barrier (BBB).
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2023)
Article
Immunology
Luca Marchetti, David Francisco, Sasha Soldati, Neda Haghayegh Jahromi, Sara Barcos, Isabelle Gruber, Javier R. Pareja, Aude Thiriot, Ulrich von Andrian, Urban Deutsch, Ruth Lyck, Remy Bruggmann, Britta Engelhardt
Summary: The migration of CD4(+) effector/memory T cells across the blood-brain barrier (BBB) is a critical step in multiple sclerosis (MS) or its animal model, EAE. Researchers identified Ackr1 as one of the main candidate genes upregulated in pMBMECs favoring transcellular T-cell diapedesis. Additionally, endothelial ACKR1 plays a role in shuttling chemokines across the BBB during EAE pathogenesis, enhancing transcellular T-cell diapedesis during autoimmune neuroinflammation.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Oncology
Federico Saltarin, Adrian Wegmuller, Leire Bejarano, Ece Su Ildiz, Pascale Zwicky, Andrej Vianin, Florentin Spadin, Klara Soukup, Vladimir Wischnewski, Britta Engelhardt, Urban Deutsch, Ines J. Marques, Martin Frenz, Johanna A. Joyce, Ruth Lyck
Summary: In this study, the researchers investigated the molecular and cellular mechanisms underlying melanoma cell extravasation across the blood-brain barrier (BBB) to prevent brain metastasis formation. They found that melanoma cells exclusively use the junctional pathway to intercalate into the BBB and that proteases play a crucial role in this process. The compromised BBB barrier properties were shown to facilitate melanoma cell extravasation both in vitro and in vivo. Preserving BBB integrity is an important measure to limit the formation of melanoma-brain metastasis.
Article
Multidisciplinary Sciences
Hui Wang, Zhiyan Xu, Ziyue Xia, Michael Rallo, Andrew Duffy, Michael P. Matise
Summary: The study demonstrates that astrocytes are the predominant cell type in the adult CNS that transduce Hh signaling, and Gli1+ Hh-responsive astrocytes have regionally distinct molecular and functional properties. Inactivation of Hh pathway in astrocytes leads to region-specific BBB defects, affecting transcytosis but not paracellular diffusion. The findings suggest that the Hedgehog pathway is required to maintain BBB properties in specific regions of the adult mammalian CNS.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Francesco Roncato, Ofer Regev, Sara W. Feigelson, Sandeep Kumar Yadav, Lukasz Kaczmarczyk, Nehora Levi, Diana Drago-Garcia, Samuel Ovadia, Marina Kizner, Yoseph Addadi, Joao C. Sabino, Yossi Ovadya, Sergio F. de Almeida, Ester Feldmesser, Gabi Gerlitz, Ronen Alon
Summary: Research indicates that reduced levels of A-type lamins in highly metastatic cancer cells do not increase their ability to pass through endothelial barriers and lung vessels, but may instead compromise lung metastasis at a later stage.
Article
Cell Biology
Idaira M. Guerrero-Fonseca, Alexander Garcia-Ponce, Eduardo Vadillo, Nathaniel L. Lartey, Hilda Vargas-Robles, Sandra Chanez-Paredes, Ramon Castellanos-Martinez, Porfirio Nava, Abigail Betanzos, Brittany M. Neumann, Kinga Penkala-Auguste, Craig T. Lefort, Michael Schnoor
Summary: Neutrophil recruitment plays a critical role in sepsis, while excessive recruitment can lead to tissue damage. The actin-binding protein HS1 has been identified as a key regulator in neutrophil extravasation and its importance in sepsis has been demonstrated in a lethal sepsis model. HS1-deficient mice showed better survival, reduced lung damage and inflammation in sepsis. The absence of HS1 attenuated systemic neutrophil recruitment and altered expression of various molecules.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Carla Rodriguez-Mogeda, Sabela Rodriguez-Lorenzo, Jiji Attia, Jack van Horssen, Maarten E. Witte, Helga E. de Vries
Summary: Multiple sclerosis is an inflammatory disease where B cells play a crucial role in the pathogenesis, migrating into the central nervous system through various routes. Understanding the routes of B cell entry into the inflamed CNS is essential for comprehending the disease.
Article
Chemistry, Multidisciplinary
Assaf Zinger, Sirena Soriano, Gherardo Baudo, Enrica De Rosa, Francesca Taraballi, Sonia Villapol
Summary: The study developed leukocyte-based biomimetic nanoparticles for direct treatment of traumatic brain injury, achieving selective targeting of the damaged brain and accumulation in peripheral organs. The therapeutic approach showed potential efficacy in reducing brain damage.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Anne-Eva van der Wijk, Theodosia Georgakopoulou, Rob Steendam, Johan Zuidema, Peter L. Hordijk, Erik N. T. P. Bakker, Ed van Bavel
Summary: Drug development for neurological diseases faces challenges due to the blood-brain barrier. This study examined the potential of biodegradable microspheres to cross the blood-brain barrier and deliver drugs to the brain tissue. The results showed that microspheres of various compositions were able to extravasate into the brain parenchyma without causing significant tissue damage.
Article
Biochemistry & Molecular Biology
Angie de la Cruz, Aubrey Hargrave, Sri Magadi, Justin A. Courson, Paul T. Landry, Wanyu Zhang, Fong W. Lam, Monica A. Bray, C. Wayne Smith, Alan R. Burns, Rolando E. Rumbaut
Summary: Platelet extravasation during inflammation is influenced by CD18, PMNs, and mast cell degranulation. Injured corneas with deficiencies in CD18 or mast cells show reduced platelet and RBC extravasation, highlighting the importance of these factors in the process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Eva M. Medina-Rodriguez, Eleonore Beurel
Summary: The blood brain barrier (BBB) is crucial for protecting the brain, and its disruption may contribute to the development of depression. Stress and inflammation play important roles in BBB dysfunction, and targeting the BBB could be a potential therapeutic approach in managing depression.
NEUROBIOLOGY OF DISEASE
(2022)
Review
Oncology
Kirit Singh, Kelly M. Hotchkiss, Kisha K. Patel, Daniel S. Wilkinson, Aditya A. Mohan, Sarah L. Cook, John H. Sampson
Summary: Glioblastoma is an immunologically 'cold' tumor characterized by low or absent numbers of tumor-infiltrating lymphocytes, which if present are exhausted and ineffective. To maximize immunotherapy efficacy in glioblastoma, it is highly desirable to enhance the trafficking of lymphocytes to the tumor. However, the process of T cell recruitment into the central nervous system is tightly regulated, involving multiple steps including interaction with the blood-brain barrier and navigating the tumor microenvironment.
Article
Gastroenterology & Hepatology
Jing Lu, Xiaobing Fan, Lei Lu, Yueyue Yu, Erica Markiewicz, Jessica C. Little, Ashley M. Sidebottom, Erika C. Claud
Summary: Maternal immune activation (MIA) derived from late gestational infection increases the risk of neurodevelopmental deficits in offspring. This study shows that MIA induced underdevelopment and dysfunction of the blood-brain barrier (BBB) in prewean mice, leading to impaired spatial learning later in life. Maternal supplementation of Limosilactobacillus reuteri (L. reuteri) rescued BBB development and cognitive function associated with BBB dysfunction.
Article
Biochemistry & Molecular Biology
Linqiang Huang, Mengting Liu, Wenqiang Jiang, Hongguang Ding, Yongli Han, Miaoyun Wen, Ya Li, Xiaoyu Liu, Hongke Zeng
Summary: This study investigated the role of bradykinin and its receptor in blood-brain barrier (BBB) injury during stroke. The results showed that high levels of bradykinin led to BBB damage, cellular death, inflammation, and increased oxidative stress. Bradykinin also inhibited the expression of Wnt3a and decreased the expression of tight junction proteins. Knockdown of the bradykinin 1 receptor significantly reduced infarct volume and inflammation in an ischemic/reperfusion rat model.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Editorial Material
Clinical Neurology
Britta Engelhardt
ACTA NEUROPATHOLOGICA
(2018)
Article
Biochemistry & Molecular Biology
Julia M. Laufer, Ruth Lyck, Daniel F. Legler
Article
Endocrinology & Metabolism
Ana B. Garcia-Martin, Pascale Zwicky, Thomas Gruber, Christoph Matti, Federica Moalli, Jens Stein, David Francisco, Gaby Enzmann, Mitchell P. Levesque, Ekkehard Hewer, Ruth Lyck
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2019)
Article
Multidisciplinary Sciences
Mariana Castro Dias, Caroline Coisne, Ivana Lazarevic, Pascale Baden, Masaki Hata, Noriko Iwamoto, David Miguel Ferreira Francisco, Michael Vanlandewijck, Liqun He, Felix A. Baier, Deborah Stroka, Remy Bruggmann, Ruth Lyck, Gaby Enzmann, Urban Deutsch, Christer Betsholtz, Mikio Furuse, Shoichiro Tsukita, Britta Engelhardt
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Gaby Enzmann, Roberto Adelfio, Aurelie Godel, Neda Haghayegh Jahromi, Silvia Tietz, Sabrina S. Burgener, Urban Deutsch, Hartmut Wekerle, Charaf Benarafa, Britta Engelhardt
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Immunology
Isabella Wimmer, Silvia Tietz, Hideaki Nishihara, Urban Deutsch, Federica Sallusto, Fabien Gosselet, Ruth Lyck, William A. Muller, Hans Lassmann, Britta Engelhardt
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Neurosciences
Mariana Castro Dias, Caroline Coisne, Pascale Baden, Gaby Enzmann, Lillian Garrett, Lore Becker, Sabine M. Hoelter, Martin Hrabe de Angelis, Urban Deutsch, Britta Engelhardt, Antonio Aguilar-Pimentel, Thure Adler, Dirk H. Busch, Nadine Spielmann, Kristin Moreth, Wolfgang Hans, Oana Amarie, Jochen Graw, Jan Rozman, Ildiko Radc, Frauke Neff, Julia Calzada-Wack, Birgit Rathkolb, Eckhard Wolf, Thomas Klopstock, Wolfgang Wurst, Johannes Beckers, Manuela Oestereicher, Gregor Miller, Holger Maier, Claudia Stoeger, Stefanie Leuchtenberger, Valerie Gailus-Durner, Helmut Fuchs
FLUIDS AND BARRIERS OF THE CNS
(2019)
Review
Biochemistry & Molecular Biology
Mariana Castro Dias, Josephine A. Mapunda, Mykhailo Vladymyrov, Britta Engelhardt
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Medicine, Research & Experimental
Zhilin Li, Emilia A. Korhonen, Arianna Merlini, Judith Strauss, Eleonoora Wihuri, Harri Nurmi, Salli Antila, Jennifer Paech, Urban Deutsch, Britta Engelhardt, Sudhakar Chintharlapalli, Gou Young Koh, Alexander Fluegel, Kari Alitalo
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Review
Neurosciences
David M. F. Francisco, Luca Marchetti, Sabela Rodriguez-Lorenzo, Eduardo Frias-Anaya, Ricardo M. Figueiredo, Peter Winter, Ignacio Andres Romero, Helga E. de Vries, Britta Engelhardt, Remy Bruggmann
FLUIDS AND BARRIERS OF THE CNS
(2020)
Article
Immunology
Patricia Hamminger, Luca Marchetti, Teresa Preglej, Rene Platcer, Ci Zhu, Anton Kamnev, Ramona Rica, Valentina Stolz, Lisa Sandner, Marlis Alteneder, Elisa Kaba, Darina Waltenberger, Johannes B. Huppa, Michael Trauner, Christoph Bock, Ruth Lyck, Jan Bauer, Loic Dupre, Christian Seiser, Nicole Boucheron, Britta Engelhardt, Wilfried Ellmeier
Summary: This study reveals the significant role of histone deacetylase 1 (HDAC1) in controlling effector CD4(+) T cell migration, preventing experimental autoimmune encephalomyelitis. HDAC1-deficient CD4(+) T cells exhibit abnormal migration and morphology on surfaces coated with integrin LFA-1 ligand ICAM-1.
JOURNAL OF AUTOIMMUNITY
(2021)
Article
Immunology
Luca Marchetti, David Francisco, Sasha Soldati, Neda Haghayegh Jahromi, Sara Barcos, Isabelle Gruber, Javier R. Pareja, Aude Thiriot, Ulrich von Andrian, Urban Deutsch, Ruth Lyck, Remy Bruggmann, Britta Engelhardt
Summary: The migration of CD4(+) effector/memory T cells across the blood-brain barrier (BBB) is a critical step in multiple sclerosis (MS) or its animal model, EAE. Researchers identified Ackr1 as one of the main candidate genes upregulated in pMBMECs favoring transcellular T-cell diapedesis. Additionally, endothelial ACKR1 plays a role in shuttling chemokines across the BBB during EAE pathogenesis, enhancing transcellular T-cell diapedesis during autoimmune neuroinflammation.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Medicine, General & Internal
Steven T. Proulx, Britta Engelhardt
Summary: The central nervous system maintains a unique relationship with the immune system through brain barriers, allowing for immune surveillance and protection of neural tissue without activating immune responses unless direct injury occurs.
JOURNAL OF INTERNAL MEDICINE
(2022)
Article
Dermatology
Andreas Dzung, Annalisa Saltarit, Natascia Tiso, Ruth Lyck, Reinhard Dummer, Mitchell P. Levesque
Summary: Inactivation of STK11 in BRAF and NRAS-mutant melanoma leads to increased proliferation and invasive phenotype. Overexpression of STK11 reverses this invasive phenotype and can be targeted by inhibitors of signal transducer and activator of transcription 3/5 and FAK.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Engineering, Electrical & Electronic
Josue Page Vizcaino, Federico Saltarin, Yury Belyaev, Ruth Lyck, Tobias Lasser, Paolo Favaro
Summary: A novel deep learning approach, LFMNet, is introduced for reconstructing confocal microscopy stacks from single light field images rapidly and accurately, enabling imaging of highly dynamic and light-sensitive events, as well as in closed-loop systems where reducing latency is crucial.
IEEE TRANSACTIONS ON COMPUTATIONAL IMAGING
(2021)