Review
Biochemistry & Molecular Biology
Martina Casarin, Alessandro Morlacco, Fabrizio Dal Moro
Summary: This review summarizes the strategies and biomaterials used in pediatric urological reconstruction, with a focus on tissue regeneration and the role of seeded cells and growth factors. It highlights the importance of these techniques in pediatric applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physiology
B. Malique Jones, Gerald C. Mingin, Nathan R. Tykocki
Summary: Mast cells and degranulation of inflammatory mediators contribute to lower urinary tract symptoms. This study found that the mast cell stimulator compound 48/80 alters urinary bladder smooth muscle contractility via urothelium-dependent inflammatory pathways, independent of mast cells. These findings suggest that compound 48/80 could be used to manage inflammatory nonneurogenic bladder hyperactivity.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Review
Medicine, General & Internal
Katarina Topoliova, Stefan Harsanyi, Lubos Danisovic, Stanislav Ziaran
Summary: Tissue engineering is important in treating neurogenic bladder dysfunction, but the translation from preclinical to clinical trials is slow and lacks standardization. This review analyzes the current status of trials and discusses future prospects based on the current state of the art.
MEDICINA-LITHUANIA
(2023)
Article
Biochemistry & Molecular Biology
Jin Kono, Masakatsu Ueda, Atsushi Sengiku, Sylvia O. Suadicani, Osamu Ogawa, Hiromitsu Negoro
Summary: The study suggests that urothelial Cx43 mediated ATP signaling and coordination of urothelial activity are essential for proper perception and regulation of responses to bladder distension in the animals' awake, active phase. This research highlights the significant role of Cx43 in bladder function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Orthopedics
Spencer E. Szczesny, David T. Corr
Summary: A breakout session was held at the 2022 ORS Tendon Section Meeting to establish guidelines for studying tendon cell and tissue cultures. This paper summarizes the outcomes of the discussion and provides recommendations for future studies.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Multidisciplinary Sciences
Tiffany Tate, Tina Xiang, Sarah E. Wobker, Mi Zhou, Xiao Chen, Hyunwoo Kim, Ekatherina Batourina, Chyuan-Sheng Lin, William Y. Kim, Chao Lu, James M. Mckiernan, Cathy Lee Mendelsohn
Summary: The differential expression of PPARg in bladder cancer subtypes plays a crucial role in tumor formation and progression. The activation of Pparg in basal bladder cells can prevent tumor formation but injury-induced differentiation of these cells into luminal cells can lead to tumor development. Understanding the molecular mechanisms behind subtype transition provides important insights for the diagnosis and treatment of bladder cancer.
NATURE COMMUNICATIONS
(2021)
Article
Urology & Nephrology
Celine Grenier, Filipa M. Lopes, Anna M. Cueto-Gonzalez, Eulalia Rovira-Moreno, Romy Gander, Benjamin W. Jarvis, Karen D. McCloskey, Alison M. Gurney, Glenda M. Beaman, William G. Newman, Adrian S. Woolf, Neil A. Roberts
Summary: Urofacial, or Ochoa, syndrome (UFS) is an autosomal recessive disease characterized by abnormal bladder contractions and undilated outflow tract, as well as abnormal facial expressions. Variants in HPSE2 and LRIG2 have been identified as the genetic causes of UFS. Experimental studies in mice with LRIG2 mutations showed abnormalities in urination and bladder nerves. Peripheral neurogenic defects may underlie bladder dysfunction in LRIG2-associated UFS.
KIDNEY INTERNATIONAL REPORTS
(2023)
Article
Engineering, Biomedical
Swati Sharma, Bikramjit Basu
Summary: This review provides an update on the different biomaterial-based strategies for restoring the dynamic function of dysfunctional bladder tissue. The perspectives of bladder tissue engineering and total bladder replacement are discussed, with a focus on scaffold-mediated regenerative medicine and the development of a biostable bladder prosthesis, respectively. The challenges and advancements in the field are highlighted, including the need for vascularization, innervation, 3D prototype fabrication, and prevention of urinary encrustation. The potential of bioelectronic medicine in urology is also explored.
Article
Biochemistry & Molecular Biology
Jia-Fong Jhang, Han-Chen Ho, Yuan-Hsiang Hsu, Yuan-Hong Jiang, Hann-Chorng Kuo
Summary: This study found that patients with recurrent urinary tract infection (rUTI) have bladder defects and elevated urine inflammatory markers. After treatment with platelet-rich plasma (PRP) injections, some patients showed improvement in bladder defects.
Review
Biochemistry & Molecular Biology
Federico Boschi, Manuela Malatesta
Summary: Bladder cancer is a common disease with a significant impact on patients' quality of life. The impermeable urothelial barrier makes the diagnosis and treatment of bladder cancer challenging. Nanotechnology provides opportunities to improve the diagnostic and therapeutic approaches by enabling the crossing of the urothelial barrier, active targeting, therapeutic agent delivery, and visualization using different imaging techniques. This article presents recent experimental applications of nanoparticle-based imaging techniques for the specific detection of bladder cancer cells, showing promising results in vivo and potential translation to clinical practice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Yan Li, Yaxiao Liu, Zhengdong Gao, Lekai Zhang, Lipeng Chen, Zonglong Wu, Qinggang Liu, Shuai Wang, Nan Zhou, Toby C. Chai, Benkang Shi
Summary: This study used single-cell RNA sequencing to comprehensively characterize the cellular anatomy of mouse bladder urothelial cells, identifying novel cell subpopulations marked by specific genes and potential progenitor cells. The findings enhance understanding of bladder urothelium biology and related bladder diseases.
CELL PROLIFERATION
(2021)
Article
Food Science & Technology
Hodan Ibrahim, Jacquie Maignel, Fraser Hornby, Donna Daly, Matthew Beard
Summary: This review discusses the current evidence of BoNT/A's effect on bladder sensation, potential mechanisms by which it exerts these effects, and recent advances in understanding its action in bladder tissue.
Article
Biotechnology & Applied Microbiology
Olimpia Ortiz-Arrabal, Jesus Chato-Astrain, Pascual Vicente Crespo, Ingrid Garzon, Maria Dolores Mesa-Garcia, Miguel Alaminos, Carolina Gomez-Llorente
Summary: In this study, the potential of maslinic acid (MA) to improve keratinocyte culture methods for skin tissue engineering was evaluated. The results showed that MA increased cell proliferation and activity without affecting cell viability. Additionally, MA upregulated gene expression and enhanced cell dissociation and explant efficiency.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Engineering, Biomedical
Wenying Wei, Honglian Dai
Summary: Osteochondral defect regeneration remains a challenging issue in the musculoskeletal system, with traditional clinical treatments showing limited efficacy. However, the development of tissue engineering has provided more promising results in regenerating damaged osteochondral tissues.
BIOACTIVE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Jesus Chato-Astrain, David Sanchez-Porras, Oscar Dario Garcia-Garcia, Claudia Vairo, Maria Villar-Vidal, Silvia Villullas, Indalecio Sanchez-Montesinos, Fernando Campos, Ingrid Garzon, Miguel Alaminos
Summary: This study evaluated the use of EGF-loaded nanostructured lipid carriers (NLC) to enhance efficiency of human skin keratinocyte primary cultures, showing that NLC-rhEGF culture medium significantly improved cell growth and gene expression.
Article
Oncology
Arianna Hustler, Ian Eardley, Jennifer Hinley, Joanna Pearson, Felix Wezel, Francois Radvanyi, Simon C. Baker, Jennifer Southgate
EXPERIMENTAL CELL RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Rhiannon V. McNeill, Andrew S. Mason, Mark E. Hodson, James W. F. Catto, Jennifer Southgate
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Pediatrics
A. Radford, J. Hinley, A. Pilborough, J. Southgate, R. Subramaniam
JOURNAL OF PEDIATRIC UROLOGY
(2019)
Editorial Material
Andrology
Sreemoti Banerjee, Jennifer Southgate
TRANSLATIONAL ANDROLOGY AND UROLOGY
(2019)
Article
Immunology
Vanessa Daza-Cajigal, Adriana S. Albuquerque, Joanna Pearson, Jennifer Hinley, Andrew S. Mason, Jens Stahlschmidt, Adrian J. Thrasher, Vibhash Mishra, Jennifer Southgate, Siobhan O. Burns
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Urology & Nephrology
Simon C. Baker, Andrew S. Mason, Jennifer Southgate
Meeting Abstract
Oncology
S. C. Baker, A. S. Mason, J. Southgate
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2020)
Article
Engineering, Biomedical
Ashley Ward, Debora Morgante, John Fisher, Eileen Ingham, Jennifer Southgate
Summary: Bladder acellular matrix has potential applications in reconstructive surgeries, but the decellularization process is complex. This study used a mechanical engineering approach to design a laser-printed flat-bed apparatus for reproducible and full-thickness bladder tissue decellularization.
BIOMEDICAL MATERIALS
(2021)
Article
Biochemical Research Methods
Chung-Yao Wang, Edmund Bergstrom, Jennifer Southgate, Jane Thomas-Oates
Summary: This study aimed to investigate the highly differentiated urothelial apical surface glycome. A method was developed to collect tissue apical surface N- and O-glycans to characterize the glycome. The findings are important for understanding urothelial differentiation and the development of urothelial diseases.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Simon C. Baker, Andrew S. Mason, Raphael G. Slip, Katie T. Skinner, Andrew Macdonald, Omar Masood, Reuben S. Harris, Tim R. Fenton, Manikandan Periyasamy, Simak Ali, Jennifer Southgate
Summary: Limited understanding of bladder cancer aetiopathology hampers progress in reducing incidence. This study provides missing mechanistic evidence linking reactivated BKPyV-infection to bladder cancer risk.
Article
Pathology
Jennifer Hinley, Rosalind Duke, Jessica Jinks, Jens Stahlschmidt, David Keene, Raimondo M. Cervellione, Imran Mushtaq, Paolo De Coppi, Massimo Garriboli, Jennifer Southgate
Summary: Bladder exstrophy (BEX) is a rare developmental abnormality that results in an open bladder plate. This study found that patient-derived BEX epithelial cells in vitro can form a barrier-forming epithelium under specific conditions. The expression of squamous KRT14 transcript is inversely related to barrier development, and transcriptional drivers of urothelial differentiation show reduced expression in squamous BEX cultures.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Andrew S. Mason, Claire L. Varley, Olivia M. Foody, Xiang Li, Katie Skinner, Dawn Walker, Tony R. Larson, Daisuke Wakamatsu, Simon C. Baker, Jennifer Southgate
Summary: Urothelium is a specialized epithelium that lines the lower urinary tract and plays a crucial role in maintaining barrier function. This study explored the role of glycerophospholipids in urothelial barrier function and demonstrated the importance of lipid mediators in tissue homeostasis. Transcriptomic analysis revealed metabolic changes during urothelial differentiation, and knocking down the LPCAT4 gene significantly affected cell proliferation and barrier function recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Simon C. Baker, Andrew S. Mason, Raphael G. Slip, Pontus Eriksson, Gottfrid Sjodahl, Ludwik K. Trejdosiewicz, Jennifer Southgate
Summary: This study found that using interferon gamma (IFN gamma) in the treatment of bladder cancer can improve patients' survival rate, while treatments targeting immune checkpoints have limited effectiveness. The research also discovered that IFN gamma can regulate the expression of immune inhibitory genes, providing new therapeutic targets to enhance immunotherapy.
Letter
Urology & Nephrology
Simon C. Baker, Andrew S. Mason, Jennifer Southgate
Article
Cell & Tissue Engineering
Debora Morgante, Anna Radford, Syed K. Abbas, Eileen Ingham, Ramnath Subramaniam, Jennifer Southgate
Summary: This study demonstrates the anti-inflammatory and tissue-integrative properties of non-crosslinked acellular matrices, providing evidence for their incorporation into surgical procedures, such as in primary complex hypospadias repair.
JOURNAL OF TISSUE ENGINEERING
(2021)