Article
Engineering, Biomedical
Joseph A. M. Steele, Axel C. Moore, Jean-Philippe St-Pierre, Seth D. McCullen, Adam J. Gormley, Conor C. Horgan, Cameron RM. Black, Christoph Meinert, Travis Klein, Siamak Saifzadeh, Roland Steck, Jiongyu Ren, Maria A. Woodruff, Molly M. Stevens
Summary: This study designed and fabricated a porous zonal microstructured scaffold that mimics the structure and mechanical properties of articular cartilage. In vitro experiments showed that chondrocyte-seeded scaffolds could accumulate extracellular matrix. Repair experiments conducted on animal models demonstrated the stable retention, osteointegration, and prolonged degradation of the scaffold. Although the histological scoring did not show superiority over the control group, this may be due to limited degradation of the implant.
Article
Engineering, Biomedical
Shuangpeng Jiang, Guangzhao Tian, Zhen Yang, Xiang Gao, Fuxin Wang, Juntan Li, Zhuang Tian, Bo Huang, Fu Wei, Xinyu Sang, Liuqi Shao, Jian Zhou, Zhenyong Wang, Shuyun Liu, Xiang Sui, Quanyi Guo, Weimin Guo, Xu Li
Summary: This study confirms that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration. The promoting effect may be related to the polarization of macrophages and inhibition of inflammatory response by hWJMSC-Exos. Additionally, hWJMSC-Exos contain miRNAs that can promote hyaline cartilage regeneration.
BIOACTIVE MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Jiang-Nan Fu, Xing Wang, Meng Yang, You-Rong Chen, Ji-Ying Zhang, Rong-Hui Deng, Zi-Ning Zhang, Jia-Kuo Yu, Fu-Zhen Yuan
Summary: This article reviews the advances in osteochondral tissue engineering and discusses various strategies including scaffold design, material types, fabrication techniques, and functional properties. Although there are still challenges and difficulties, research in this field is of great importance for repairing OC defects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Runfeng Cao, Anqi Zhan, Zheng Ci, Cheng Wang, Yunlang She, Yong Xu, Kaiyan Xiao, Huitang Xia, Li Shen, Depeng Meng, Chang Chen
Summary: The study developed a biomimetic biphasic scaffold for osteochondral defect repair, controlling the composition, stiffness, and pore size to promote adhesion, proliferation, chondrogenic, and osteogenic differentiation of bone marrow stem cells. In vitro and in vivo results showed that the biphasic scaffold significantly enhanced concomitant repair of superficial cartilage and subchondral bone, demonstrating its promising osteochondral repair effect.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Wenhui Chu, Gaowei Hu, Lin Peng, Wei Zhang, Zhe Ma
Summary: The study demonstrated that adCDMs, derived from antler decellularized cartilage, are rich in collagen and GAGs, with good biosafety and biocompatibility. When implanted into rabbit articular cartilage defects, adCDMs showed excellent therapeutic effects in promoting cartilage regeneration. These findings highlight the potential of adCDMs as a novel source for developing cartilage-regenerating repair materials.
JOURNAL OF BIOLOGICAL ENGINEERING
(2021)
Review
Chemistry, Multidisciplinary
Shenqiang Wang, Sheng Zhao, Jicheng Yu, Zhen Gu, Yuqi Zhang
Summary: 3D printing technology has great potential in the field of tissue engineering, allowing the fabrication of biomimetic tissue structures for personalized therapy of various diseases. This review introduces the recently developed 3D printing techniques and summarizes their applications in cartilage, bone, and osteochondral tissue regeneration.
Article
Engineering, Biomedical
Yiyang Du, Menghan Chen, Jing Jiang, Lei Wang, Gang Wu, Jianying Feng
Summary: This study evaluates the effectiveness of human salivary histatin-1-functionalized methacrylic gelatin scaffold in controlling osteochondral tissue regeneration and repair in rabbits with major temporomandibular joint dimensional abnormalities.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Review
Medicine, Research & Experimental
Ziyang Zhou, Jin Cui, Shunli Wu, Zhen Geng, Jiacan Su
Summary: Osteoarthritis is a common joint disease that causes physical and psychological harm to patients and burdens the healthcare system. Tissue engineering has been focusing on cartilage repair, with silk fibroin as a promising biomaterial due to its unique properties and capabilities for functional modification.
Article
Materials Science, Multidisciplinary
Hairui Suo, Yu Chen, Jiali Liu, Ling Wang, Mingen Xu
Summary: A novel biphasic scaffold was designed and fabricated using HAp and PCL through a multi-nozzle 3D printing technique, showing mechanical properties between pure HAp and PCL scaffolds. Finite element simulation demonstrated a biomimetic strengthened structure of the biphasic scaffold under compression, suitable for osteochondral tissue engineering applications.
JOURNAL OF MATERIALS SCIENCE
(2021)
Article
Engineering, Biomedical
Gagan K. Jalandhra, Thomas G. Molley, Tzong-tyng Hung, Iman Roohani, Kristopher A. Kilian
Summary: This study developed a unique printing platform that integrates soft and hard materials concurrently through freeform printing of mineralized constructs within tunable micro-gel suspensions containing living cells. By tuning the microgel stiffness and filler content, chondrogenesis and osteogenesis can be differentially directed within the same construct, enabling the fabrication of osteochondral interfaces in a single step. This versatile one-pot biofabrication approach has the potential to aid in bone disease modeling and tissue engineering.
ACTA BIOMATERIALIA
(2023)
Review
Pharmacology & Pharmacy
Xinwei Li, Shanshan Sun, Xiaoming Wang, Wei Dong
Summary: The incidence of osteoarthropathy is rising, affecting human quality of life. Articular cartilage lacks self-repair ability. Osteochondral tissue engineering offers a new solution for repair. Polyester materials have potential applications in osteochondral regeneration.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Engineering, Biomedical
Deborah L. Dorcemus, Hyun S. Kim, Syam P. Nukavarapu
Summary: The development of a biodegradable matrix with uniquely arranged bone and cartilage supporting phases, by incorporating hydroxyapatite into a PLGA template and loading different growth factors onto different parts of the scaffold, facilitated spatial distribution of chondrogenesis and osteogenesis. Experimental results showed that this inverse gradient matrix can promote spatial differentiation of human mesenchymal stem cells into cartilage and bone cells within the scaffold.
BIOMEDICAL MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Martina Tortorici, Ansgar Petersen, Klara Ehrhart, Georg N. Duda, Sara Checa
Summary: Mechanical and biological factors are known to play key roles in osteochondral defect healing, but the underlying principles and how they can be applied in treatment strategies are still largely unknown. A computational approach was used to investigate the impact of scaffold mechanical and architectural properties on osteochondral defect healing, revealing that scaffold material elastic modulus and structural stability are crucial for supporting the repair process.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Pharmacology & Pharmacy
Markel Lafuente-Merchan, Sandra Ruiz-Alonso, Fatima Garcia-Villen, Idoia Gallego, Patricia Galvez-Martin, Laura Saenz-del-Burgo, Jose Luis Pedraz
Summary: This article reviews the advantages of using 3D bioprinting techniques for osteochondral regeneration, discusses the commonly used biomaterials, cell types, and active molecules, and considers the most recent promising results.
Article
Biotechnology & Applied Microbiology
Chih-Hsiang Fang, Yi-Wen Lin, Chung-Kai Sun, Jui-Sheng Sun
Summary: This study developed an extracellular matrix-based biphasic scaffold loaded with two small molecule drugs to promote osteochondral regeneration. The scaffold showed excellent biocompatibility in vitro, with mesenchymal stem cells adhering, proliferating, and differentiating. In an in vivo rat model, the scaffold demonstrated effective regeneration of osteochondral defects.
BIOENGINEERING-BASEL
(2023)
Article
Materials Science, Ceramics
Ke Liu, Xiaopei Wu, Jiawei Liu, He Yang, Meng Li, Tong Qiu, Honglian Dai
Summary: This study successfully achieved a customized, large-size and high-strength bioactive ceramic scaffold through SLA technology. The interaction effects of laser power, spot spacing, and scanning rate on the curing behavior of HA slurry were investigated. A compact shell/porous core with different volume ratios was fabricated to meet the requirement of high mechanical strength for load-bearing parts. Additionally, the effect of different microchannel structural units on vascularization was explored using a modeling algorithm. Overall, SLA ceramic printing technology can be used to develop HA composite scaffolds with large size, high strength, and excellent biological properties for bioceramic repair of large bone defects.
CERAMICS INTERNATIONAL
(2023)
Article
Engineering, Biomedical
Qing Li, Wenbin Liu, Wen Hou, Xiaopei Wu, Wenying Wei, Jiawei Liu, Yihe Hu, Honglian Dai
Summary: This study proposes a photothermal double-layer biomimetic periosteum with neurovascular coupling. The outer layer is a conventional electrospinning membrane to prevent soft tissue invasion, and the inner layer is an oriented nanofiber membrane to promote cell recruitment and angiogenesis. The bionic periosteum simulates the microenvironment of extracellular matrix and periosteum regeneration, ultimately promoting bone regeneration.
MATERIALS TODAY BIO
(2023)
Article
Nanoscience & Nanotechnology
Cuicui Li, Yilun Cai, Jinrong Hu, Jiawei Liu, Honglian Dai, Qingfang Xu, Chitengfei Zhang, Xian Zhang, Kai Liu, Marina L. Kosinova, Takashi Goto, Rong Tu, Song Zhang
Summary: This study fabricated a SiC/graphene composite film via laser chemical vapor deposition (LCVD) as a dopamine (DA) sensor. The film exhibited excellent electrocatalytic activity, low detection limit, and high sensitivity. Its three-dimensional porous network structure enhanced electron transfer rate and catalytic active site exposure, while reducing interfacial resistance. Moreover, the film showed good biocompatibility for biomedical applications.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Xiaoxuan Wang, Pu Chen, He Yang, Jiawei Liu, Rong Tu, Hai-Tao Feng, Honglian Dai
Summary: The research team successfully synthesized an aggregation-induced emission-active luminogen (AIEgen) named 4BC with efficient reactive oxygen species (ROS) generation and loaded it into 3D bioactive scaffolds. Good in situ imaging performance for the implanted scaffolds was achieved through simple UV light irradiation. Among them, the 4BC@TMP scaffold composed of trimagnesium phosphate (TMP) showed excellent bactericidal ability for Escherichia coli and Staphylococcus aureus in vitro and resisted bacterial inflammation in vivo through photodynamic action. This study confirms the potential of AIEgen-based 3D scaffolds for bioimaging and antibacterial applications.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Yanan Zhao, Liangcong Hu, Honglian Dai, Pengzhen Zhuang, Kai Huang, Wen Hou, Jiawei Liu, Rong Tu, Takashi Goto
Summary: Infected bone defect has become a serious complication in orthopedic surgery due to bacterial biofilm formation. In this study, drug-loaded hollow mesoporous ferrite nanoparticles were prepared and incorporated into calcium magnesium phosphate cement, forming a Trap-Capture-Kill system to efficiently kill bacteria and inhibit biofilm formation. Under an alternating magnetic field, the composites exhibited acute magnetic hyperthermal capacity and recruited free bacteria. The permeability of bacterial cell membrane was increased by reactive oxygen species damage, allowing the drugs encapsulated in the nanoparticles to penetrate. The bacteria were then efficiently killed through magnetothermal effect, ROS damage, and adsorption damage. Furthermore, the antibacterial system showed excellent pro-osteogenesis and pro-angiogenic capacity, promoting vascularized bone regeneration in vivo.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Jinhai Xie, Shuangyu Tian, Hanning Zhang, Congshu Feng, Yingchao Han, Honglian Dai, Lesan Yan
Summary: Designing nano-drug delivery vehicles that respond to stimuli from the tumor microenvironment is crucial in cancer therapy. Enzyme-responsive nano-drug delivery systems are particularly effective as they target tumor-specific and highly expressed enzymes, resulting in increased drug release at the target sites and reduced toxicity to normal tissues. NQO1, an important reductase associated with cancer, is overexpressed in certain cancer cells, making the design of nanocarriers with high selectivity and responsiveness to NQO1 significant for tumor diagnosis and treatment.
Article
Chemistry, Physical
Haifei Kang, Wenying Wei, Lingshun Sun, Ran Yu, Erkang Yang, Xiaopei Wu, Honglian Dai
Summary: The researchers report a modular conjugation method for designing internal boron-nitrogen coordinated boronic ester (IBNCB) hydrogels by constructing polymers with phenylboronic acid or N,N-bis(2-hydroxyethyl) moieties based on amides. These hydrogels exhibited unique pH responsiveness, enhanced hydrolysis stability, bidirectional pH-tunable mechanical properties, and a lowered and tunable pH for gelation. They also showed sol-gel transitional responsiveness to reactive oxygen species (ROS), glucose, and temperature. The amide-based modular conjugation could be exploited to generate a theoretically limitless number of novel IBNCB materials.
CHEMISTRY OF MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Puxin Weng, Kun Liu, Meng Yuan, Guo-Quan Huang, Kun Wu, Xiaofan Yang, Honglian Dai, Weigang Lu, Dan Li
Summary: This article reports a simple and general method to prepare ZIF-91 porous liquid (ZIF-91-PL), which has permanent porosity, good fluidity, and fine dispersion, and has potential applications in drug delivery.
Article
Engineering, Biomedical
Yanan Zhao, Honglei Kang, Xiaopei Wu, Pengzhen Zhuang, Rong Tu, Takashi Goto, Feng Li, Honglian Dai
Summary: A drug carrier system composed of gelatin-coated hollow mesoporous silica nanoparticles loaded with pro-osteogenic parathyroid (PTH) and anti-osteoclastogenic alendronate (ALN) is constructed and compounded into calcium magnesium phosphate cement (MCPC). It enhances osteoporotic bone regeneration in ovariectomized rats by promoting osteogenic differentiation, inhibiting osteoclastogenesis, and accelerating vascularization. The MCPC/HMSNs@ALN-PTH/GM demonstrates a synergistic effect on osteogenesis, osteoclastogenesis, and angiogenesis, holding potential for other biomaterials design and clinical management.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Qinghua Hou, Kun Liu, Chenxi Lian, Jiawei Liu, Wenying Wei, Tong Qiu, Honglian Dai
Summary: Bacterial infection, prolonged inflammation, and insufficient angiogenesis are the main challenges for effective wound repair. In this study, a stretchable, remodeling, self-healing, and antibacterial multifunctional composite hydrogel was developed for infected wound healing. The hydrogel was prepared using tannic acid (TA) and phenylboronic acid-modified gelatin (Gel-BA) through hydrogen bonding and borate ester bonds and incorporated iron-containing bioactive glasses (Fe-BGs) to achieve GTB composite hydrogels. Animal experiments showed that the GTB hydrogels remarkably accelerated infected wound healing by improving granulation tissue formation, collagen deposition, and the formation of nerves and blood vessels while decreasing inflammation. This hydrogel holds immense potential for wound dressing applications.
Article
Materials Science, Multidisciplinary
Meng Li, Jiawei Jiang, Wenbin Liu, Xiaolong Huang, Xiaopei Wu, Wenying Wei, Hao Zhu, Jinyong Zhang, Jun Xiao, Honglian Dai
Summary: This study designs a bioadaptive ceramic scaffold for bone defect repair, incorporating bioactive components and bionic structures, which exhibits good mechanical properties and bone regeneration capacity.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Biomedical
Youzi Zhao, Honglei Kang, Yuhao Xia, Lingshun Sun, Feng Li, Honglian Dai
Summary: The study combines photothermal therapy and copper ion release to construct a multifunctional scaffold for treating infected bone defects. It uses programmed temperature control to kill bacteria and promote bone tissue growth, while gradually releasing copper ions to enhance the antibacterial effect and promote angiogenesis. This approach reduces damage to healthy cells and tissues.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Energy & Fuels
Biliang Lin, Hongxiang Chen, Wenying Wei, Jiling Zhang, Minghao Wu, Weigang Li, Weiyan Zhu, Yifan Zhang, Yong Wang
Summary: A self-assembly method was developed to synthesize cuprous chloride/copper chloride hydroxide composites for investigating their hydrogen sulfide (H2S) removal performances. It was found that the Cu intermediate product by Fe reduction reacted with CuCl2 to form CuCl, which then self-assembled with CuCl2 and H2O to generate copper chloride hydroxides shell layer, leading to increased H2S adsorption capacity. Extensive analyses revealed the sulfidation reaction mechanism of CCCCHs, showing that the decomposition of CCHs enhanced the H2S treatment capacity substantially.
Article
Engineering, Biomedical
Jianqiu Yang, Zhenzhen Huang, Jiang Tan, Jingye Pan, Shixuan Chen, Wenbing Wan
Summary: By functionalizing a decellularized pomelo peel with an adhesive hydrogel and antibacterial materials, the hybrid wound dressing can effectively inhibit bacterial infection, promote granulation tissue formation and angiogenesis, and reduce scar formation during wound healing.
BIOACTIVE MATERIALS
(2024)
Article
Engineering, Biomedical
Yong Mei, Xiaohua Qin, Zhenyu Yang, Shiyao Song, Xiaoting Liu, Chong Wu, Jieying Qian, Xiaowan Huang, Yunjiao Zhang, Weiling He
Summary: This article presents a novel nanoparticle for treating KRAS-TP53 co-mutation in gastrointestinal cancers. The nanoparticle can degrade mutant p53 proteins (mutp53) and deliver the drug AMG510 to inhibit mutant KRAS and mutp53 signaling pathways. The results show that the nanoparticle can effectively reduce cell proliferation and migration, and demonstrate remarkable therapeutic efficacy in a tumor-bearing mouse model.
BIOACTIVE MATERIALS
(2024)
