Article
Biochemistry & Molecular Biology
Riko Kitazawa, Ryuma Haraguchi, Yukihiro Kohara, Sohei Kitazawa
Summary: The RANK receptor regulates the differentiation and function of osteoclasts through direct binding of NFATc1. The RANKL signal positively regulates RANK transcription, promoting accelerated terminal differentiation of osteoclast precursors to mature osteoclasts.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Fujun Jin, Yexuan Zhu, Meijing Liu, Rongze Wang, Yi Cui, Yanting Wu, Gang Liu, Yifei Wang, Xiaogang Wang, Zhe Ren
Summary: In this study, Babam2 was identified as an essential negative regulator of osteoclast formation and bone resorption. It interacts with Hey1 to inhibit Nfatc1 transcription, thus negatively regulating osteoclastogenesis. Targeting Babam2 may be a novel therapeutic approach for osteoclast-related bone diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Immunology
Chao Ma, Liang Mo, Zhangzheng Wang, Deqiang Peng, Chi Zhou, Wei Niu, Yuhao Liu, Zhenqiu Chen
Summary: By modulating multiple signaling pathways, DTI can inhibit osteoclast formation and prevent estrogen-deficiency osteoporosis. This makes DTI a promising candidate for the treatment of osteoporosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Shuyue Yang, Dezhi Song, Ziyi Wang, Yuangang Su, Junchun Chen, Yansi Xian, Jian Huang, Jing Li, Jiake Xu, Jinmin Zhao, Qian Liu
Summary: This study found that AZD inhibits the generation, function, and ROS-scavenging enzyme activity of mature osteoclasts, suggesting its potential as a new therapeutic agent for osteoporosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Engineering, Biomedical
Jiaolong Wang, Qin Zhao, Liangliang Fu, Shihang Zheng, Can Wang, Litian Han, Zijian Gong, Ziming Wang, Hua Tang, Yufeng Zhang
Summary: CD301b(+) macrophages play an important role in the angiogenesis of calcium phosphate bioceramics, and their VEGF expression is regulated by store-operated calcium entry and calcineurin signals through the NFATc1/VEGF axis.
BIOACTIVE MATERIALS
(2022)
Article
Endocrinology & Metabolism
Xiaofang Zhu, Hao Yuan, Ouyang Ningjuan, Carroll Ann Trotman, Thomas E. Van Dyke, Jake Jinkun Chen, Guofang Shen
Summary: Corticotomy is effective but invasive for accelerating tooth movement in orthodontic treatment. This study found that 6-shogaol can promote osteoclast differentiation and induce transient osteopenia, accelerating tooth movement non-invasively. These results suggest a potential alternative method for faster tooth movement in orthodontic treatment.
JOURNAL OF BONE AND MINERAL METABOLISM
(2021)
Article
Engineering, Biomedical
Zhangling Nie, Zhiqiao Hu, Xiaodong Guo, Yu Xiao, Xian Liu, Joost D. de Bruijn, Chongyun Bao, Huipin Yuan
Summary: Innate immune responses, specifically M2 macrophages and osteoclastogenesis, play important roles in material-induced bone formation. The study found that M0 macrophages fused to osteoclasts when treated with M-CSF, RANKL, and IL-4, and this osteoclast formation was enhanced with IL-4 treatment. M2 macrophages and osteoclasts produced osteogenic factors that induced osteogenic differentiation of osteogenic precursor cells and led to bone formation.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Xiaodong Guo, Mingzheng Li, Wenting Qi, Hetian Bai, Zhangling Nie, Zhiqiao Hu, Yu Xiao, Joost D. de Bruijn, Chongyun Bao, Huipin Yuan
Summary: The study revealed that osteoinductive calcium phosphate ceramic triggers key cellular events: macrophages colonize within hours to days, M2 macrophage polarization occurs within 7 days, osteoclastogenesis mainly occurs in weeks (e.g. in 2 weeks), and bone formation eventually arises thereafter (after 4 weeks). Such findings provide insights into material-induced bone formation and pave the way for further exploration of mechanisms triggered by osteoinductive materials.
ACTA BIOMATERIALIA
(2021)
Article
Veterinary Sciences
Xiang-Dong Su, Seo Y. Yang, Saroj K. Shrestha, Yunjo Soh
Summary: AS-A2 inhibits osteoclastogenesis induced by RANKL and reduces the activation of NF-κB and MAPK pathways. Therefore, AS-A2 may be a promising therapy for osteolytic diseases such as osteoporosis.
JOURNAL OF VETERINARY SCIENCE
(2022)
Article
Medicine, Research & Experimental
Tingting Li, Dongbiao Qiu, Qingjiao Chen, Apeng Yang, Junmin Chen, Zhiyong Zeng
Summary: This study explored the role of Na+/Ca2+ exchanger 1 (NCX1) in the development of multiple myeloma (MM) and myeloma bone disease (MBD). The study found that NCX1 was highly expressed in bone marrow tissues of MM patients and was positively correlated with serum calcium levels and the percentage of BM CD138(+) cells. Inhibition of NCX1 reduced cell viability and induced apoptosis in MM cells. High extracellular calcium levels increased intracellular calcium levels in MM cells and promoted the expression of NCX1 and genes related to osteoclastogenesis. NCX1 overexpression accelerated osteoclastogenesis, while NCX1 knockdown or suppression had the opposite effect. The study further revealed that NCX1 regulated osteoclast differentiation in MM through the JNK/c-Fos/NFATc1 signaling pathway.
CLINICAL AND EXPERIMENTAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Hongzhou Shen, Yu Zhuang, Chenglong Zhang, Changru Zhang, Yuan Yuan, Hongbo Yu, Jiawen Si, Guofang Shen
Summary: This study investigated the value of applying BMP2-loaded calcium phosphate cement in restoring large-scale alveolar bone defects. The results showed that BMP2-CPC can induce favorable bone regeneration and remodeling, accompanied by active osteoclast formation and optimized biomaterial resorption. Osteoclasts played a multifaceted role in the bone regeneration process by promoting osteogenic differentiation and biomaterial remodeling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Sijia Chen, Qiong Wang, Felipe Eltit, Yubin Guo, Michael Cox, Rizhi Wang
Summary: The study developed a novel nanostructured calcium phosphate coating using AiM technique on a porous membrane, enabling in vitro simulation of osteoclastic resorption. The coating not only facilitates drug delivery but can also be used for drug screening.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Immunology
Prashant S. Giri, Ankit H. Bharti, Rasheedunnisa Begum, Mitesh Dwivedi
Summary: NFATs and FOXP3 are associated with impaired regulatory T-cells (Tregs) in generalized vitiligo (GV). This study investigated the calcium mediated NFATc1 signaling pathway and its effect on Treg suppressive capacity in GV. The results showed altered calcium homeostasis in GV Tregs, leading to decreased calcineurin and NFATc1 activity. Elevated GSK-3 beta activity and DYRK1A transcripts were also involved in reduced NFATc1 activity in GV Tregs. Calcium treatment enhanced Treg-mediated suppression and increased Tregs associated cytokines, suggesting the potential of calcium-NFATc1 signaling pathway as a therapeutic target for improving Treg function in GV.
Article
Immunology
Yin Xiao, Musga Qureischi, Lena Dietz, Martin Vaeth, Subrahmanya D. Vallabhapurapu, Stefan Klein-Hessling, Matthias Klein, Chunguang Liang, Anika Koenig, Edgar Serfling, Anja Mottok, Tobias Bopp, Andreas Rosenwald, Mathias Buttmann, Ingolf Berberich, Andreas Beilhack, Friederike Berberich-Siebelt
Summary: Inhibition of NFATc1 SUMOylation can fine-tune T cell responses, promote T reg expansion, and suppress autoreactive or alloreactive immune responses.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Oncology
Heng Liu, Qian Sun, Shuai Chen, Long Chen, Wenming Jia, Juan Zhao, Xiulian Sun
Summary: Glioblastoma is an aggressive brain tumor that is resistant to chemotherapy and radiotherapy, with complex and unknown pathogenesis. Research shows that targeting DYRK1A to reduce NFATC1 stability is a promising therapeutic approach for GBM treatment, inhibiting glioblastoma migration.
Article
Rheumatology
Vivian E. Saper, Michael J. Ombrello, Adriana H. Tremoulet, Gonzalo Montero-Martin, Sampath Prahalad, Scott Canna, Chisato Shimizu, Gail Deutsch, Serena Y. Tan, Elaine F. Remmers, Dimitri Monos, Timothy Hahn, Omkar K. Phadke, Elaine Cassidy, Ian Ferguson, Vamsee Mallajosyula, Jianpeng Xu, Jaime S. Rosa Duque, Gilbert T. Chua, Debopam Ghosh, Ann Marie Szymanski, Danielle Rubin, Jane C. Burns, Lu Tian, Marcelo A. Fernandez-Vina, Elizabeth D. Mellins, Jill A. Hollenbach
Summary: Drug reaction with eosinophilia and systemic symptoms (DRESS) can occur in Still's disease patients treated with IL-1/IL-6 inhibitors, with features such as eosinophilia, liver enzyme elevation, and persistent rash. HLA-DRB1*15 haplotypes are significantly enriched in Still's disease with DRESS. Pre-prescription HLA typing and vigilance for serious reactions to these drugs are recommended.
ANNALS OF THE RHEUMATIC DISEASES
(2022)
Article
Biochemical Research Methods
Niclas Olsson, Wei Jiang, Lital N. Adler, Elizabeth D. Mellins, Joshua E. Elias
Summary: This study investigates the effects of DO:DM ratios on peptide presentation by creating a panel of HLA-DR4-expressing APC lines and analyzing over 10,000 unique DR4-presented peptides. The findings provide insights into the characteristics that influence peptide presentation and have implications for HLA-II prediction algorithms and research strategies.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Cell Biology
Saborni Chakraborty, Joseph C. Gonzalez, Benjamin L. Sievers, Vamsee Mallajosyula, Srijoni Chakraborty, Megha Dubey, Usama Ashraf, Bowie Yik-Ling Cheng, Nimish Kathale, Kim Quyen Thi Tran, Courtney Scallan, Aanika Sinnott, Arianna Cassidy, Steven T. Chen, Terri Gelbart, Fei Gao, Yarden Golan, Xuhuai Ji, Seunghee Kim-Schulze, Mary Prahl, Stephanie L. Gaw, Sacha Gnjatic, Thomas U. Marron, Miriam Merad, Prabhu S. Arunachalam, Scott D. Boyd, Mark M. Davis, Marisa Holubar, Chaitan Khosla, Holden T. Maecker, Yvonne Maldonado, Elizabeth D. Mellins, Kari C. Nadeau, Bali Pulendran, Upinder Singh, Aruna Subramanian, Paul J. Utz, Robert Sherwood, Sheng Zhang, Prasanna Jagannathan, Gene S. Tan, Taia T. Wang
Summary: A damaging inflammatory response in severe COVID-19 is associated with early non-neutralizing, afucosylated IgG antibodies specific to SARS-CoV-2. Human IgG-Fc-gamma receptor interactions regulate inflammation in the lung, and antibodies elicited by mRNA SARS-CoV-2 vaccines have reduced inflammatory potential.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Debopam Ghosh, Tho D. Pham, Padma P. Nanaware, Deepanwita Sengupta, Lital N. Adler, Caiyun G. Li, Xiao He, Mary E. O'Mara, Aaron B. Kantor, Khoa D. Nguyen, Yang Yang, Laurence C. Eisenlohr, Peter E. Jensen, Leonore A. Herzenberg, Lawrence J. Stern, Scott D. Boyd, Eliver E. B. Ghosn, Elizabeth D. Mellins
Summary: The absence of H2-M protein affects the survival and immunoglobulin selection of B-1 cells, and also alters the specificity of the B-1 cell clonal repertoire.
Article
Rheumatology
Guangbo Chen, Gail H. Deutsch, Grant S. Schulert, Hong Zheng, SoRi Jang, Bruce Trapnell, Pui Y. Lee, Claudia Macaubas, Katherine Ho, Corinne Schneider, Vivian E. Saper, Adriana Almeida de Jesus, Mark A. Krasnow, Alexei Grom, Raphaela Goldbach-Mansky, Purvesh Khatri, Elizabeth D. Mellins, Scott W. Canna
Summary: This study investigated the immunopathology and identified biomarkers in systemic JIA, MAS, and SJIA-LD by measuring over 1,300 analytes in sera. The proteome alterations in active systemic JIA and MAS overlapped, with novel elevations in heat-shock proteins and glycolytic enzymes.
ARTHRITIS & RHEUMATOLOGY
(2022)
Article
Allergy
Hesam Movassagh, Mary Prunicki, Abhinav Kaushik, Xiaoying Zhou, Diane Dunham, Eric M. M. Smith, Ziyuan He, German R. Aleman R. Muench, Minyi Shi, Annika K. K. Weimer, Shu Cao, Sandra Andorf, Amir Feizi, Michael P. P. Snyder, Pejman Soroosh, Elizabeth D. D. Mellins, Kari C. C. Nadeau
Summary: This study found that exposure to fine particulate matter (PM2.5) leads to specific alterations in monocytes, especially in children with asthma. Further mechanistic research revealed that fine particulate matter induces innate trained immunity in circulating monocytes, resulting in an enhanced inflammatory response. These findings suggest that the alterations of monocytes may serve as an immune signature for pediatric asthma, and pollution-induced trained immunity could be a potential therapeutic target for asthmatic children.
Article
Developmental Biology
Agnieszka Kalinowski, Lu Tian, Reenal Pattni, Hanna Ollila, Maroof Khan, Cindy Manko, Melissa Silverman, Meiqian Ma, Laurie Columbo, Bahare Farhadian, Susan Swedo, Tanya Murphy, Mats Johnson, Elisabeth Fernell, Christopher Gillberg, Margo Thienemann, Elizabeth D. Mellins, Douglas F. Levinson, Alexander E. Urban, Jennifer Frankovich
Summary: Pediatric Acute-onset Neuropsychiatric Syndrome (PANS) is a sudden-onset neuropsychiatric disorder with an increased prevalence of co-morbid autoimmune illness, particularly arthritis. PANS patients with low C4B copy number are at higher risk for subsequent Juvenile Idiopathic Arthritis (JIA) diagnosis. There is a possible association between low C4B and PANS age of onset, suggesting a potential role of C4B in different types of arthritis.
DEVELOPMENTAL NEUROSCIENCE
(2023)
Article
Developmental Biology
Allison Vreeland, Denise Calaprice, Noga Or-Geva, Richard E. Frye, Dritan Agalliu, Herbert M. Lachman, Christopher Pittenger, Stefano Pallanti, Kyle Williams, Meiqian Ma, Margo Thienemann, Antonella Gagliano, Elizabeth Mellins, Jennifer Frankovich
Summary: Post-infectious neuroinflammation is implicated in acute onset obsessive-compulsive disorder. This review article outlines the evidence supporting the role of neuroinflammation in these disorders, including animal models, imaging studies, and research on sleep and metabolites. The authors encourage interdisciplinary collaboration to further research on clinical syndromes with neuropsychiatric manifestations.
DEVELOPMENTAL NEUROSCIENCE
(2023)
Article
Rheumatology
Kunjan Khanna, Hui Yan, Muneshwar Mehra, Nidhi Rohatgi, Gabriel Mbalaviele, Elizabeth D. Mellins, Roberta Faccio
Summary: This study reveals the regulatory mechanism of NLRP3 inflammasome in systemic juvenile idiopathic arthritis (sJIA). The down-regulation of TMEM178 levels is associated with disease activity and may serve as a marker for identifying patients who would benefit from inflammasome targeting.
ARTHRITIS & RHEUMATOLOGY
(2023)
Article
Engineering, Biomedical
Fuqiang Ren, Feifei Wang, Ani Baghdasaryan, Ying Li, Haoran Liu, Rusiou Hsu, Chuchu Wang, Jiachen Li, Yeteng Zhong, Felix Salazar, Chun Xu, Yingying Jiang, Zhuoran Ma, Guanzhou Zhu, Xiang Zhao, Kerry Kaili Wong, Richard Willis, K. Christopher Garcia, Anna Wu, Elizabeth Mellins, Hongjie Dai
Summary: This study reports a cancer vaccine that can track and image immune cells non-invasively in vivo, providing insights into the immune responses induced by vaccination. The vaccine rapidly migrates to lymph nodes through lymphatic vessels and eradicates pre-existing tumors with two doses. The use of nanoparticle-based vaccines and imaging probes emitting infrared light has important implications for the design and optimization of immunotherapies.
NATURE BIOMEDICAL ENGINEERING
(2023)
Editorial Material
Health Care Sciences & Services
Jennifer Frankovich, Heer Nanda, Elizabeth D. D. Mellins, Harumi Jyonouchi, Richard G. G. Boles, Stephen J. J. Walker, John Gaitanis, Richard E. E. Frye
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Cell Biology
Justine Maller, Terry Morgan, Mayu Morita, Frank McCarthy, Yunshin Jung, Katrin J. Svensson, Joshua E. Elias, Claudia Macaubas, Elizabeth Mellins
Summary: The study suggests that intercellular communication mediated by extracellular vesicles plays an important role in the pathogenesis of systemic juvenile idiopathic arthritis. The number and cellular sources of extracellular vesicles differ between the active and inactive states of the disease and healthy controls.
JOURNAL OF LEUKOCYTE BIOLOGY
(2023)
Meeting Abstract
Rheumatology
Mariana Correia Marques, Danielle Rubin, Emily Shuldiner, Elizabeth Schmitz, Elizabeth Baskin, Andrew Patt, Alexei Grom, Dirk Foell, Marco Gattorno, John Bohnsack, Rae Yeung, Sampath Prahalad, Elizabeth Mellins, Jordi Anton, Dirk Foell, Marco Gattorno, John Bohnsack, Rae Yeung, Sampath Prahalad, Elizabeth Mellins, Jordi Anton, Claudio Len, Sheila Oliveira, Patricia Woo, Seza Ozen, Michael Ombrello
ARTHRITIS & RHEUMATOLOGY
(2022)
Meeting Abstract
Rheumatology
Hiroto Nakano, Emily Shuldiner, Anne Hinks, Marc Sudman, Elaine Remmers, Colleen Satorius, Elizabeth Schmitz, Victoria Arthur, Patricia Woo, Alexei Grom, Dirk Foell, John Bohnsack, Marco Gattorno, Seza Ozen, Sampath Prahalad, Rae Yeung, Elizabeth Mellins, Sheila Oliveira, Jordi Anton, Claudio Len, Carol Lake, Ly-Lan Bergeron, Michelle Millwood, Estefania de los Santos, Mariana Correia Marques, Carl Langefeld, Susan Thompson, Wendy Thomson, Michael Ombrello
ARTHRITIS & RHEUMATOLOGY
(2022)
Meeting Abstract
Immunology
Shamma Rahman, Achia Khatun, Fabian Gaertner, Claudia Macaubas, Nytzia Licona, Laurie Columbo, Bahare Farhadian, Theresa Willett, Margo Thienemann, Jennifer Frankovich, Elizabeth Mellins
JOURNAL OF CLINICAL IMMUNOLOGY
(2022)