Article
Biochemistry & Molecular Biology
Ngai Ting Chan, Junfeng Huang, Gui Ma, Hao Zeng, Kristine Donahue, Yidan Wang, Lingjun Li, Wei Xu
Summary: CTR9 is a scaffold subunit in PAFc complex and plays a crucial role in controlling H3K27me3 levels and genomic distribution mediated by PRC2. Loss of CTR9 leads to increased H3K27me3 levels, expanded recruitment of PRC2 on chromatin, and a shift from less active PRC2.2 to more active PRC2.1, resulting in hypersensitivity of breast cancer cells to PRC2 inhibitors.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yang Yi, Yanqiang Li, Chao Li, Longxiang Wu, Dongyu Zhao, Fuxi Li, Ladan Fazli, Rui Wang, Long Wang, Xuesen Dong, Wei Zhao, Kaifu Chen, Qi Cao
Summary: CDCA8 overexpression is associated with poor clinical outcome in patients with prostate cancer, and EZH2 is responsible for CDCA8 activation in PCa.
Editorial Material
Biochemistry & Molecular Biology
Tyler. J. J. Reich, Peter. W. W. Lewis
Summary: A study on drug-resistant lymphomas reveals the existence of a 'methylation index' that enables cancer cells to maintain optimal H3K27me3 levels, highlighting the significance of comprehending tumor adaptation to chromatin changes and drug-resistance mutations.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xifeng Xiong, Xudong Lai, Jinli Zhang, Qingqi Meng, Pengzhen Wang, Shengnan Qin, Wei Liu, Yongxuan Wang, Zhuo Yao, Di Wang, Xiaojian Li, Zhihe Liu, Haixiong Miao
Summary: Ovarian cancer is the second leading cause of cancer death in women worldwide. This study identifies FBP1 as a potential target for ovarian cancer treatment, as it is closely associated with the development of ovarian cancer and positively correlated with the expression of EZH2. Knockdown of FBP1 suppresses tumor formation and cisplatin resistance in ovarian cancer cells, suggesting its role in promoting cisplatin resistance. The physical binding between FBP1 and EZH2 is also validated in this study. These findings highlight the importance of FBP1 as a novel target for the development of ovarian cancer treatment.
BIOSCIENCE REPORTS
(2022)
Article
Cell Biology
Yamato Fukui, Yasushi Hirota, Shizu Aikawa, Akihiko Sakashita, Ryoko Shimizu-Hirota, Norihiko Takeda, Chihiro Ishizawa, Rei Iida, Tetsuaki Kaku, Tomoyuki Hirata, Takehiro Hiraoka, Shun Akaeda, Mitsunori Matsuo, Yutaka Osuga
Summary: Infertility affects 15% of couples worldwide, with recurrent implantation failure (RIF) being a major issue in IVF-ET programs. This study revealed that a uterine gene network regulated by the polycomb repressive complex 2 (PRC2) controls embryo implantation. Dysregulation of PRC2 components and target genes was observed in patients with RIF. Knockout mice studies showed that stromal Ezh2 plays a crucial role in female fertility. This research highlighted the importance of the EZH2-PRC2-H3K27me3 axis in preparing the endometrium for successful embryo invasion.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Xufen Yu, Jun Wang, Weida Gong, Anqi Ma, Yudao Shen, Chengwei Zhang, Xijuan Liu, Ling Cai, Jing Liu, Gang Greg Wang, Jian Jin
Summary: This passage focuses on the noncanonical function of EZH2 in the development of multiple myeloma (MM), and proposes a novel therapeutic strategy, pharmacological degradation of EZH2, for treating EZH2-dependent MM.
Article
Biology
Le Ni, Bowen Lin, Yanping Zhang, Lingjie Hu, Jianghua Lin, Fengmei Fu, Meiting Shen, Can Li, Lei Chen, Jian Yang, Dan Shi, Yi-Han Chen
Summary: Histone modifications play crucial roles in the pathogenesis of myocardial ischaemia/reperfusion (I/R) injury. In this study, transcriptome and epigenome of histone modifications were integrated to characterize epigenetic signatures after I/R injury. Disease-specific histone mark alterations were mainly found in H3K27me3-, H3K27ac-, and H3K4me1-marked regions 24 and 48 h after I/R.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Multidisciplinary Sciences
Bo Zhang, Chen Zhao, Wenchen Shen, Wei Li, Yue Zheng, Xiangfei Kong, Junbao Wang, Xudong Wu, Tao Zeng, Ying Liu, Yan Zhou
Summary: The hippocampus plays a crucial role in learning and memory, and its development requires precise coordination of cell patterning, proliferation, differentiation, and migration. In this study, it was found that KDM2B mediates a gene repressive program that controls fate specification and cell migration in the hippocampus, affecting its morphology and related behaviors.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Lihu Gong, Xiuli Liu, Lianying Jiao, Xin Yang, Andrew Lemoff, Xin Liu
Summary: The authors identify SUZ12 as a cellular substate of casein kinase 2 (CK2) and highlight its role in promoting PRC2 function in cell identity maintenance during stem cell differentiation. This phosphorylation-dependent mechanism stimulates PRC2 enzymatic activity, enhancing its binding to substrates and promoting gene repression. Loss of this phosphorylation impairs PRC2 recruitment and compromises its ability to maintain differentiated cell identity.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Anna K. Kania, Madeline J. Price, Lou-Ella George-Alexander, Dillon G. Patterson, Sakeenah L. Hicks, Christopher D. Scharer, Jeremy M. Boss
Summary: This study demonstrates that H3K27 demethylases inhibit B cell differentiation.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Anna K. Kania, Madeline J. Price, Lou-Ella George-Alexander, Dillon G. Patterson, Sakeenah L. Hicks, Christopher D. Scharer, Jeremy M. Boss
Summary: In this study, it was found that the H3K27 demethylases restrain B cell differentiation. Mice with simultaneous genetic deletion of UTX and JMJD3 showed a significant increase in plasma cell formation and increased ATP synthesis capacity. Furthermore, UTX was found to regulate chromatin accessibility of known repressors of PC fate.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ka-Wing Fong, Jonathan C. Zhao, Xiaodong Lu, Jung Kim, Andrea Piunti, Ali Shilatifard, Jindan Yu
Summary: PALI1 is a newly identified accessory protein that plays a role in advanced prostate cancer. It competes with JARID2 for binding to the PRC2 core subunit SUZ12 and interacts with the H3K9 methyltransferase G9A, forming a unique super-complex involved in dual H3K9/K27 methylation and gene repression. PALI1 and G9A promote prostate cancer cell proliferation and invasion, and drive tumor growth in xenograft models.
Article
Multidisciplinary Sciences
Ayushi Verma, Akhilesh Singh, Manish Pratap Singh, Mushtaq Ahmad Nengroo, Krishan Kumar Saini, Saumya Ranjan Satrusal, Muqtada Ali Khan, Priyank Chaturvedi, Abhipsa Sinha, Sanjeev Meena, Anup Kumar Singh, Dipak Datta
Summary: The selective hyper-activation of functional EZH2 (H3K27me3) alters TNBC metastatic landscape and drives peritoneal metastasis, suggesting a potential role for trimethylation function of EZH2 in TNBC.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Serena Buontempo, Pasquale Laise, James M. Hughes, Sebastiano Trattaro, Vivek Das, Chantal Rencurel, Giuseppe Testa
Summary: This study reveals the role of EZH2-mediated H3K27me3 in neuronal differentiation. Using a mouse model and transcriptomic analysis, it was found that the loss of EZH2 leads to dysregulation of transcriptional circuitries related to neuronal differentiation and synaptic plasticity, as well as the expression of GABAergic genes during glutamatergic neuron differentiation.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Oncology
Chong Wang, Tao Wang, Kang-Jing Li, Ling-Hong Hu, Yue Li, Yu-Zhong Yu, Tao Xie, Sha Zhu, Du-Jiang Fu, Yang Wang, Xian-Zi Zeng, Feng-Ping Liu, Hong Chen, Zhe-Sheng Chen, Ning-Han Feng, Jinghua Liu, Yong Jiang, Shan-Chao Zhao
Summary: The SETD4 protein plays a crucial role in prostate cancer by catalyzing histone lysine methylation and suppressing NUPR1 transcription, leading to the inhibition of Akt signaling pathway and preventing PCa development.
Article
Oncology
Dora Mihaly, Zsolt Matula, Yi-Che Changchien, Gergo Papp, Peter Tatrai, Zoltan Sapi
Article
Medicine, Research & Experimental
Dora Mihaly, Gergo Papp, Zsolt Mervai, Andrea Reszegi, Peter Tatrai, Gabor Szaloki, Johanna Sapi, Zoltan Sapi
EXPERIMENTAL BIOLOGY AND MEDICINE
(2018)
Article
Pharmacology & Pharmacy
Peter Tatrai, Patrick Schweigler, Birk Poller, Norbert Domange, Roelof de Wilde, Imad Hanna, Zsuzsanna Gaborik, Felix Huth
DRUG METABOLISM AND DISPOSITION
(2019)
Article
Oncology
Andrea Reszegi, Zsolt Horvath, Hajnalka Feher, Barnabas Wichmann, Peter Tatrai, Ilona Kovalszky, Kornelia Baghy
FRONTIERS IN ONCOLOGY
(2020)
Review
Pharmacology & Pharmacy
Peter Tatrai, Peter Krajcsi
Article
Biochemistry & Molecular Biology
Peter Hollosi, Lorand Vancza, Katalin Karaszi, Katalin Dobos, Balint Peterfia, Eniko Tatrai, Peter Tatrai, Tibor Szarvas, Sandor Paku, Laszlo Szilak, Ilona Kovalszky
Article
Biochemistry & Molecular Biology
Andrea Reszegi, Zsolt Horvath, Katalin Karaszi, Eszter Regos, Victoria Postnikova, Peter Tatrai, Andras Kiss, Zsuzsa Schaff, Ilona Kovalszky, Kornelia Baghy
Article
Oncology
Andrea Reszegi, Katalin Karaszi, Gabor Toth, Kristof Rada, Lorand Vancza, Lilla Turiak, Zsuzsa Schaff, Andras Kiss, Laszlo Szilak, Gabor Szabo, Gabor Petovari, Anna Sebestyen, Katalin Dezso, Eszter Regos, Peter Tatrai, Kornelia Baghy, Ilona Kovalszky
Summary: Syndecan-1 plays a dual role in tumor development, either promoting or inhibiting tumors depending on the cancer type. In the context of liver cancer, transgenic expression of human syndecan-1 delayed tumor formation by downregulating lipid metabolism, inhibiting mTOR and beta-catenin pathways, and activating Foxo1 and p53 transcription factors. This oncoprotective effect was mediated by a beneficial modulation of lipid metabolism and molecular signaling pathways involved in cell cycle regulation and intermediary metabolism.
Review
Pharmacology & Pharmacy
Peter Tatrai, Franciska Erdo, Gabriella Dornyei, Peter Krajcsi
Summary: The interaction with the main reabsorptive urate transporter URAT1 seems to be dominant in determining the net effect of a drug on SU levels. In vitro transporter interaction studies can predict the effects of drugs on SU levels.
Review
Cell Biology
Lorand Vancza, Peter Tatrai, Andrea Reszegi, Kornelia Baghy, Ilona Kovalszky
Summary: SPOCK1, 2, and 3 are matricellular proteoglycans without a structural role, and their specific functions and mechanisms are not fully understood. SPOCK1 plays important roles in neural synapses, neuromuscular junctions, and the blood-brain barrier, and it has been found to have deleterious effects on tumor progression. However, its physiological functions remain poorly understood and further research is needed.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Peter Tatrai, Fanni Gergely
Summary: This study shows the critical role of CDK5RAP2 and centrosomes in spindle formation during blood production. Lack of CDK5RAP2 leads to defective erythroid differentiation and the emergence of macrocytic anemia. The study suggests that disruption of centrosome and spindle function could contribute to the development of macrocytic anemias.
Review
Pharmacology & Pharmacy
Peter Tatrai, Franciska Erdo, Peter Krajcsi
Summary: Bile acids and bile salts (BA/BS) are transported by influx and efflux transporters on hepatocytes, which play crucial roles in bile flow and liver injury. Inhibition of these transporters, especially BSEP, should be tested in early drug development to minimize the risk of drug-induced liver injury (DILI). In vitro test systems for these transporters employ membrane vesicles and whole cells. In addition, cellular toxicity tests using hepatocytes, assessments of mitochondrial toxicity and accumulation of reactive oxygen species (ROS), and inhibition of FXR function are included in pharmaceutical testing panels.
Article
Pharmacology & Pharmacy
Peter Tatrai, Csilla Temesszentandrasi-Ambrus, Tamas Varga, Zsuzsanna Gaborik
Summary: Variation in the methodology of in vitro transporter inhibition assays leads to wide divergence in reported IC50/Ki data. Preincubation of inhibitors with transporters can significantly affect the inhibitory potency, but current guidelines do not specifically recommend this practice. Inhibition assays involving solute carrier transporters show that preincubation without extracellular protein leads to significant changes in IC50, indicating the importance of considering protein-free preincubation in these assays.
DRUG METABOLISM AND DISPOSITION
(2023)
Article
Biochemistry & Molecular Biology
Gabor Petovari, Gabor Toth, Lilla Turiak, Anna L. Kiss, Krisztina Paloczi, Anna Sebestyen, Adrian Pesti, Andras Kiss, Kornelia Baghy, Katalin Dezso, Tibor Fule, Peter Tatrai, Ilona Kovalszky, Andrea Reszegi
Summary: This study reveals tumor type-dependent communication between hepatoma cells and fibroblasts, exploring various communication channels and identifying liver cancer-specific miRNAs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Cell Biology
J. Tischer, P. Tatrai, D. Adams, F. Gergely
MOLECULAR BIOLOGY OF THE CELL
(2018)