Article
Chemistry, Multidisciplinary
Youcef Chakib Hacene, Alexis Loiseau, Vanessa Dos Passos Maio, Philippe Grenier, Elodie Boisselier, Nicolas Bertrand
Summary: This study presents an immunoprecipitation method using AntiPEG antibodies to extract different types of PEGylated systems, showcasing its potential and unique perspective in understanding the interactions of nanoparticles with biological systems.
Article
Biology
Meng Zhao, Niels Banhos Danneskiold-Samsoe, Livia Ulicna, Quennie Nguyen, Laetitia Voilquin, David E. Lee, James P. White, Zewen Jiang, Nickeisha Cuthbert, Shrika Paramasivam, Ewa Bielczyk-Maczynska, Capucine Van Rechem, Katrin J. Svensson
Summary: The secreted protein Ism1 mitigates diabetes by increasing glucose uptake in adipocytes and skeletal muscles through the activation of the PI3K-Akt pathway. Phosphoproteomic analysis reveals overlapping and distinct signaling pathways of Ism1 and insulin. Ism1 affects protein translation, the mTOR pathway, and muscle function through unknown phosphorylation sites, and its ablation leads to altered proteostasis and reduced muscle protein content.
Article
Chemistry, Multidisciplinary
Stuart T. Johnston, Matthew Faria
Summary: Designing nano-engineered particles for targeted delivery of therapeutic and diagnostic agents is a challenging task. This study presents a machine learning framework that can interpret particle-cell interactions from experimental data, providing insights into the design choices. The framework reveals consistent models of particle-cell interactions for different particle-cell pairs, highlighting the importance of nonlinear saturation effects. Additionally, the framework facilitates quantitative evaluation of particle design choices by providing robust estimates of particle performance.
Article
Chemistry, Physical
Maria Martinez-Negro, Guillermo Gonzalez-Rubio, Emilio Aicart, Katharina Landfester, Andres Guerrero-Martinez, Elena Junquera
Summary: This article discusses the formation of protein corona (PC) on colloidal nanoparticles (NPs) and its impact on the biological behavior of NPs. The focus is on the mechanisms of PC formation and strategies to control PC formation to ensure the successful application of NPs in medicine.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Yadileiny Portilla, Sara Mellid, Alberto Paradela, Antonio Ramos-Fernandez, Neus Daviu, Laura Sanz-Ortega, Sonia Perez-Yague, Maria P. Morales, Domingo F. Barber
Summary: In the study, it was found that different coatings did not affect the composition of the protein corona on iron oxide nanoparticles. Enzymes with hydrolase activity in the corona may explain the degradation of coatings when in contact with biological media. Coatings primarily determine the endocytic pathways used for nanoparticle internalization by cells.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Wid Mekseriwattana, Tipparat Thiangtrongjit, Onrapak Reamtong, Patompon Wongtrakoongate, Kanlaya Prapainop Katewongsa
Summary: This study analyzed the behavior of superparamagnetic iron oxide nanoparticles (SPIONs) with two different surface coatings using proteomic analyses. The results showed that the choice of coating affected the adsorption of serum proteins and the interaction between SPIONs and cells. This is important for optimizing the design and clinical applications of SPIONs.
Article
Chemistry, Physical
Liangjia Qiu, Ying Zhang, Genxia Wei, Chen Wang, Yinhua Zhu, Tong Yang, Zheng Chu, Peng Gao, Guangqing Cheng, Ang Ma, Yin Kwan Wong, Junzhe Zhang, Chengchao Xu, Jigang Wang, Huan Tang
Summary: Nanoparticles have great potential in biomedicine due to their unique physical and chemical properties. When nanoparticles enter biological fluids, they interact with proteins and form a protein corona. Understanding and characterizing the protein corona is crucial for the clinical translation of nanomedicine.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
James Magnus Rae, Barbara Jachimska
Summary: This study revealed that the interaction between BSA and G5.5 PAMAM dendrimers is driven by both electrostatic and hydrophobic forces. The binding alters the structure, charge distribution, and secondary structure of proteins.
Article
Chemistry, Analytical
Joanna Xiuzhu Xu, Md Siddik Alom, Nicholas C. Fitzkee
Summary: An effective intensity-based reference using N-15 tryptophan as an external reference for H-1-N-15 2D NMR experiments is presented in this study to quantify competitive protein adsorption onto nanoparticle surfaces. The referencing system validated the binding capacity of single protein onto gold NPs and accurately quantified signals from protein mixtures. The proposed Trp reference enables the monitoring of competitive adsorption of different proteins in situ and in real time, highlighting the importance of studying protein-NP interactions in protein mixtures using H-1-N-15 2D NMR techniques.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Huayan Yang, Sijia Lu, Shengkun Wang, Lihong Liu, Bo Zhu, Shaoning Yu, Shouning Yang, Junbiao Chang
Summary: The study demonstrated that time-related protein corona on nanoparticles can influence macrophage polarization, inducing M2 phenotype at early stages and M1 phenotype at later stages. Proteomic analysis showed constant changes in protein corona compositions with significant increase of immune proteins after 12 hours of incubation, playing a key role in modulating macrophage polarization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Environmental Sciences
Stephanie Chaousis, Frederic D. L. Leusch, Colin J. Limpus, Amanda Nouwens, Liesbeth J. Weijs, Antonia Weltmeyer, Adrian Covaci, Jason P. van de Merwe
Summary: All seven species of sea turtle are facing increasing pressures from human activities that are impacting their health. Changes in blood proteins can indicate adverse health outcomes. This study examines the protein abundance in sea turtle plasma, compares protein expression between different populations, and investigates markers of contaminant exposure.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Zhu Wang, Qiong Deng, Yanli Gu, Min Li, Ying Zhang, Qiyi Hu, Shenping Zhang, Xisheng Wang, Hui Liang
Summary: This study investigated the gene expression changes in the kidney of a rat model with kidney stones. The results showed dysregulated genes related to injury, apoptosis, immune response, solute carriers, transporters, and metabolic factors. These findings contribute to a better understanding of the pathogenesis of nephrolithiasis and could potentially lead to the development of new strategies for prevention and treatment.
Article
Biochemistry & Molecular Biology
Susana P. Mejia, Richard da Costa Marques, Katharina Landfester, Jahir Orozco, Volker Mailaender
Summary: Encapsulation of itraconazole in functionalized nanoparticles allows for targeted therapy with reduced doses. Protein corona formation on the surfaces of nanoparticles affects their interactions with cells. PEGylated nanoparticles improve the antifungal effect of itraconazole by reducing non-specific protein adsorption.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Plant Sciences
Shujun Zhou, Xiaoke Yin, Zhitao Liang, Peter J. Hylands, Zhongzhen Zhao, Jun Yuan, Jingzheng Song, Yunxia Li, Cheng Peng, Qihe Xu
Summary: This study compared the antifibrotic activities of Scutellariae Radix extracts and flavonoids, and conducted proteomic analysis on selected flavonoids. The results showed that baicalein was one of the most promising antifibrotic flavonoids, and SR methanolic extract had stronger antifibrotic effects compared to the aqueous extract.
Review
Nanoscience & Nanotechnology
Evan P. Stater, Ali Y. Sonay, Cassidy Hart, Jan Grimm
Summary: Nanoparticles, beyond their engineered functions, can induce specific biological effects, which may have implications for the development and clinical application of nanomedicine.
NATURE NANOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Bejan Hamawandi, Hazal Batili, Moon Paul, Sedat Ballikaya, Nuzhet I. Kilic, Rafal Szukiewicz, Maciej Kuchowicz, Mats Johnsson, Muhammet S. Toprak
Summary: This study presents rapid and effective methods for synthesizing nanostructured bismuth telluride materials with promising TE performance using microwave volume heating in a short reaction time. The use of water as a solvent is as effective as ethylene glycol, providing a greener reaction medium. Characterization techniques, including XRD, SEM/TEM, XPS and zeta potential, were used to evaluate crystal structure, morphology, crystallinity, microstructure and surface chemistry of the materials. The low thermal conductivity values and high ZT values demonstrate the potential impact of these materials, developed with energy and time efficiency, in advancing TE technology with minimal environmental impact.
Article
Chemistry, Multidisciplinary
Giovanni M. Saladino, Nuzhet I. Kilic, Bertha Brodin, Bejan Hamawandi, Idris Yazgan, Hans M. Hertz, Muhammet S. Toprak
Summary: A hybrid material combining optical and X-ray fluorescence properties was presented as a bioimaging contrast agent. Rhodium core NPs were synthesized using a microwave-assisted hydrothermal method, showing potential as X-ray Fluorescence Computed Tomography (XFCT) contrast agents.
Article
Materials Science, Multidisciplinary
M. A. Almessiere, B. Unal, Y. Slimani, H. Gungunes, M. S. Toprak, N. Tashkandi, A. Baykal, M. Sertkol, A. Trukhanov, A. Yildiz, A. Manikandan
Summary: Ce-Dy co-doping has significant effects on the crystal structure, optical, and magnetic properties of Ni-Co spinel ferrite microspheres. Increasing Ce3+-Dy3+ content enhances the magnetic capabilities of the ferrite microspheres. The AC conductivity and dielectric properties are also influenced by the Ce-Dy content, with the dielectric properties strongly depending on the frequency.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2021)
Article
Pharmacology & Pharmacy
Ahmed Raslan, Jesus Ciriza, Ana Maria Ochoa de Retana, Maria Luisa Sanjuan, Muhammet S. Toprak, Patricia Galvez-Martin, Laura Saenz-del-Burgo, Jose Luis Pedraz
Summary: The study focused on the impact of protein-coated reduced graphene oxide on the conductivity of hydrogels, with results indicating that collagen had better adsorption capacity and enhanced conductivity.
Article
Biochemical Research Methods
Husen M. Umer, Enrique Audain, Yafeng Zhu, Julianus Pfeuffer, Timo Sachsenberg, Janne Lehtio, Rui Branca, Yasset Perez-Riverol
Summary: The pypgatk package and pgdb workflow have been implemented to create proteogenomics databases based on ENSEMBL resources. The tools can generate protein sequences from different types of transcripts and take into account the impact of genomic variants on protein sequences. Using these tools, researchers have reanalyzed public datasets and identified a significant number of novel protein sequences.
Article
Nanoscience & Nanotechnology
Jose F. Serrano-Claumarchirant, Bejan Hamawandi, Adem B. Ergul, Andres Cantarero, Clara M. Gomez, Pankaj Priyadarshi, Neophytos Neophytou, Muhammet S. Toprak
Summary: This study focuses on the development of large-area thermoelectric films using hybrid materials. By blending TE materials with polymers and using a specific solvent, durable polymer, and additive in the ink formulation, the researchers successfully fabricated large-area homogeneous hybrid TE films with improved conductivity and TE power factor.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Materials Science, Multidisciplinary
Y. Slimani, M. A. Almessiere, A. Demir Korkmaz, A. Baykal, A. Manikandan, H. Gungunes, M. S. Toprak
Summary: This study focused on the impact of Ga-Gd co-substitution on the structural, magnetic features and hyperfine interactions of nanosized NiCuZn spinel ferrites. The results showed that the substitution of Ga and Gd influenced the crystallite and particle sizes, as well as the cation distribution, of the spinel ferrites. Magnetometry measurements revealed changes in coercivity and saturation magnetization with the substitution of Ga and Gd. Superparamagnetic-to-ferromagnetic transition was observed below the blocking temperature.
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
(2022)
Article
Materials Science, Multidisciplinary
Z. Lalegani, S. A. Seyyed Ebrahimi, B. Hamawandi, L. La Spada, H. Batili, M. S. Toprak
Summary: An epsilon-negative metamaterial (ENM) with core@shell nanoparticles was designed. The optimal performance was achieved by growing a 10 nm SiO2 shell on 54 nm Ag nanoparticles.
MATERIALS CHEMISTRY AND PHYSICS
(2022)
Article
Biochemical Research Methods
Taner Arslan, Yanbo Pan, Georgios Mermelekas, Mattias Vesterlund, Lukas M. Orre, Janne Lehtio
Summary: The subcellular localization of a protein is crucial for understanding its functions. In this study, a mass spectrometry- and bioinformatics-based pipeline was developed to generate a comprehensive resource for protein subcellular localization in multiple human cancer cell lines. The protocol includes a detailed wet-lab procedure, quantitative MS-data analysis, machine learning-based classification, and visualization of the output. The pipeline was evaluated using different peptide pre-fractionation approaches and proved to be straightforward and robust, with the entire process being completed within 1-2 weeks.
Article
Chemistry, Physical
Hazal Batili, Bejan Hamawandi, Adem Bjorn Ergul, Muhammet Sadaka Toprak
Summary: This study aimed to assemble thermoelectric films with pre-defined morphology and surface chemistry on non-conductive substrates using electrophoretic deposition (EPD). Bi2Te3 nanoparticles were synthesized and used for the deposition of thermoelectric films on glass substrates. The addition of a molecular linker helped reduce resistance and improve the performance of the films. The measured Seebeck coefficient and power factor indicated the potential application of this technology to large area thermoelectric films.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Materials Science, Multidisciplinary
Huseyin Tombuloglu, Yassine Slimani, Sultan Akhtar, Moneerah Alsaeed, Guzin Tombuloglu, Munirah A. Almessiere, Muhammet S. Toprak, Huseyin Sozeri, Abdulhadi Baykal, Ismail Ercan
Summary: This study investigated the translocation of four different sizes of iron oxide nanoparticles in pumpkins. Only the 30-50 nm nanoparticles were significantly translocated to the leaves, showing size-dependent translocation.
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Giovanni Marco Saladino, Carmen Vogt, Bertha Brodin, Kian Shaker, Nuzhet Inci Kilic, Kenth Andersson, Marie Arsenian-Henriksson, Muhammet Sadaka Toprak, Hans Martin Hertz
Summary: In this study, hybrid ruthenium-decorated superparamagnetic iron oxide nanoparticles were designed as contrast agents for both magnetic resonance imaging (MRI) and X-ray fluorescence computed tomography (XFCT). These nanoparticles were synthesized through a one-pot polyol hot injection method. In vivo preclinical studies showed the possibility of correlative bioimaging using these contrast agents. The complementary nature of MRI and XFCT allowed for accurate localization and high specificity and resolution in nanoparticle detection, suggesting potential applications for early tumor diagnosis, improved long-term treatment monitoring, and enhanced radiotherapy planning.
Article
Biotechnology & Applied Microbiology
Govind Gupta, Bejan Hamawandi, Daniel J. Sheward, Ben Murrell, Leo Hanke, Gerald McInerney, Magda Blosi, Anna L. Costa, Muhammet S. Toprak, Bengt Fadeel
Summary: Silver nanoparticles (Ag NPs) have been found to effectively inhibit SARS-CoV-2 infection, particularly the unmodified nanoparticles. This study also showed that the Ag NPs retained their antiviral properties even after pre-incubation with pulmonary surfactant. Additionally, the Ag NPs disrupted the secondary structure of the spike protein of SARS-CoV-2, while ionic control (AgNO3) and TiO2 nanoparticles did not have the same effect. Moreover, the Ag NPs were non-cytotoxic to human lung epithelial cells and nasal epithelial cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Giovanni Marco Saladino, Ronak Kakadiya, Shaquib Rahman Ansari, Alexandra Teleki, Muhammet Sadaka Toprak
Summary: Superparamagnetic iron oxide nanoparticles (SPIONs) play a dominant role in various biomedical applications, including magnetic separation, drug delivery, diagnostics, and hyperthermia treatments. In this study, superparamagnetic nanoclusters (SP-NCs) with high unit magnetization and diameters up to 400 nm were synthesized using conventional or microwave-assisted solvothermal methods. The choice of synthesis route and capping agent significantly influenced the particle size, SP-NC size, surface chemistry, and magnetic properties. The SP-NCs were then coated with a silica shell doped with a fluorophore to provide fluorescence properties and high stability. The heating efficiency studies demonstrated the potential of these synthesized SP-NCs in hyperthermia treatment. The enhanced magnetically-active content, fluorescence, magnetic property, and heating efficiency of SP-NCs offer promising opportunities for biomedical applications.
NANOSCALE ADVANCES
(2023)
Article
Chemistry, Multidisciplinary
Anna Luisa Costa, Magda Blosi, Andrea Brigliadori, Ilaria Zanoni, Simona Ortelli, Felice Carlo Simeone, Serena Delbue, Sarah D'Alessandro, Silvia Parapini, Claudia Vineis, Alessio Varesano, Muhammet S. Toprak, Bejan Hamawandi, Davide Gardini
Summary: This study explores the antiviral and antibacterial properties of quaternized hydroxyethyl cellulose-stabilized silver nanoparticles (Ag-HEC) in different environments. The results show the potential application of Ag-HEC in protective clothing and nasal masks, providing a broad-spectrum antimicrobial solution.
ENVIRONMENTAL SCIENCE-NANO
(2022)
