Editorial Material
Multidisciplinary Sciences
Deepak Srivastava
Summary: The cytokine meteorin-like (METRNL) has been found to improve cardiac function in mice after a heart attack.
Article
Multidisciplinary Sciences
Alice Yu, Yuanyuan Li, Irene Li, Michael G. Ozawa, Christine Yeh, Aaron E. Chiou, Winston L. Trope, Jonathan Taylor, Joseph Shrager, Sylvia K. Plevritis
Summary: The study introduces a graph-based algorithm REMI that predicts ligand-receptor (LR) interactions and reconstructs interactomes from RNA sequencing data. By leveraging single-cell transcriptomics data for cell type resolution and identifying LR prognostic signatures among tumor-stroma-immune subpopulations, the study confirmed an interaction associated with LUAD progression.
Article
Thermodynamics
Kun Wang, Shuai Lin, Zezhou Ran
Summary: In this study, the chemical looping process of hydrogen sulfide oxidation by metal oxides was investigated through thermodynamic simulation, focusing on sulfur formation. Elements such as iron, copper, and manganese were screened as oxygen carriers, and the feasibility of relevant reactions was analyzed. Thermodynamic equilibrium analysis was conducted to determine the effects of temperature, pressure, and reactant ratio on sulfur formation, and the optimal conditions for V2O5, PbO2, and Pb2O3 were identified.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2023)
Article
Chemistry, Inorganic & Nuclear
Nancy Sharma, Vinod Kumar, D. Amilan Jose
Summary: A ruthenium nitrosyl complex (1 •NO) and 1 •NO incorporated phospholipid-based liposomes (Lip-1 •NO) were developed for selective colorimetric detection of H2S. The probe 1middotNO interacts with H2S and releases NO, with detection limits of 0.31 μM and 0.45 μM for 1middotNO and Lip-1middotNO, respectively. The sensing of H2S was confirmed by DAF-FM DA assay, ESI-MS, FT-IR spectroscopy, and UV-Vis spectroscopy.
DALTON TRANSACTIONS
(2023)
Review
Cell Biology
Jinliang Huang, Sipeng Wu, Pengcheng Wang, Geng Wang
Summary: Mitochondria are not only the main hubs for cellular energy production, but also play important roles in biosynthesis pathways and signaling. Mitochondrial biosynthesis requires collaboration of nuclear and mitochondrial gene expression systems, and rapid responses to changes inside and outside the cells. Recent studies suggest that non-coding RNAs may contribute to communication between mitochondria and other cellular compartments.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Rheumatology
Maximilian Kugler, Mirjam Dellinger, Felix Kartnig, Lena Mueller, Teresa Preglej, Leonhard X. Heinz, Elisabeth Simader, Lisa Goeschl, Stephan E. Puchner, Sebastian Weiss, Lisa E. Shaw, Matthias Farlik, Wolfgang Weninger, Giulio Superti-Furga, Josef S. Smolen, Guenter Steiner, Daniel Aletaha, Hans P. Kiener, Myles J. Lewis, Costantino Pitzalis, Anela Tosevska, Thomas Karonitsch, Michael Bonelli
Summary: Activated FLS play a critical role in synovial tissue remodeling and interaction with immune cells in rheumatoid arthritis (RA). Co-culture experiments demonstrated that cytokine-activated FLS enhance T cell activation, proliferation, and differentiation. Transcriptomic analysis revealed a correlation between FLS cytokine signatures and infiltrating CD4(+) T cells in RA synovial tissue. ICAM1 was identified as an important mediator in TNF-mediated FLS-T cell interaction.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Multidisciplinary Sciences
Qing Lu, Yonghong Zhang, Joakim Hellner, Caterina Giannini, Xiangyu Xu, Jarne Pauwels, Qian Ma, Wim Dejonghe, Huibin Han, Brigitte Van de Cotte, Francis Impens, Kris Gevaert, Ive De Smet, Jiri Frim, Daniel Martinez Molina, Eugenia Russinova
Summary: In this study, the authors applied the CETSA MS method to intact Arabidopsis thaliana cells and identified the thermal proteome of GSK3 inhibitor bikinin. They found that GSK3 regulates the auxin efflux carrier PIN1, and highlighted the cross-talk between brassinosteroid and auxin signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Barbara Choromanska, Piotr Mysliwiec, Tomasz Kozlowski, Magdalena Luba, Piotr Wojskowicz, Jacek Dadan, Katarzyna Makarewicz, Anna Zalewska, Mateusz Maciejczyk, Hanna Myliwiec, Katarzyna Choronnanska
Summary: This study found elevated levels of nitrosative stress, inflammation, and hypoxia biomarkers in patients with adrenal tumors. Nitrosative stress was associated with inflammation and hypoxia in different types of adrenal masses.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Sara Danielli, Zhongming Ma, Eirini Pantazi, Amrendra Kumar, Benjamin Demarco, Fabian A. Fischer, Usha Paudel, Jillian Weissenrieder, Robert J. Lee, Sebastian Joyce, J. Kevin Foskett, Jelena S. Bezbradica
Summary: CALHM6, a membrane ion channel, promotes cell-cell crosstalk at neuronal synapses via ATP release and is highly expressed in immune cells. It has been linked to NK cell anti-tumor activity, but its mechanism of action and broader functions in the immune system remain unclear.
Review
Oncology
Jiahua Liu, Runzi Zheng, Yanghuan Zhang, Shuting Jia, Yonghan He, Jing Liu
Summary: This review provides an overview of the molecular mechanisms, microenvironmental factors, and therapeutic strategies of skin aging and melanoma, and discusses potential common targets between melanoma and cell senescence.
Article
Biochemistry & Molecular Biology
Francois Maingret, Laurent Groc
Summary: The study shows that GABA(A)Rs and D5Rs form dynamic surface clusters in the hippocampus, and disrupting their interaction affects receptor diffusion and synaptic GABA(A)R levels. This rapid lateral redistribution leads to a significant depression in GABAergic currents and alters the timing of long-term potentiation at glutamatergic synapses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Laxmi Dhungel, Lindsey Burcham, Joo Youn Park, Harshini Devi Sampathkumar, Albert Cudjoe, Keun Seok Seo, Heather Jordan
Summary: Buruli ulcer is a neglected tropical disease caused by Mycobacterium ulcerans, with mycolactone as a major virulence factor. In rural West Africa, where the disease is endemic, infected lesions can be colonized by quorum sensing bacteria without typical pathology. The pathogenesis of M. ulcerans may involve synergistic or antagonistic mechanisms within a polymicrobial network. Mycolactone was found to impact S. aureus metabolism and virulence gene expression, providing insight into interspecific interactions that may be useful for treatment and prevention.
SCIENTIFIC REPORTS
(2021)
Article
Polymer Science
Fan Rong, Tengjiao Wang, Kun Wang, Qian Zhou, Haowei Peng, Peng Li
Summary: Hydrogen sulfide (H2S) has been widely studied for its therapeutic effects. However, achieving controlled H2S delivery is crucial due to its short physiological half-life and dose-dependent effects. This study developed di-para-substituted S-aroylthiooximes (p-diSATOs) as linkers to improve the stability and efficiency of H2S release in polymeric micelles. The core-cross-linked micelle (CCM) prepared through this method showed rapid initial H2S release followed by sustained release, demonstrating its potential for accelerating wound healing in a murine burn wound model.
Review
Cell Biology
Gregory Livshits, Alexander Kalinkovich
Summary: The increase in lifespan and proportion of older adults has resulted in a rise in age-associated chronic diseases, such as sarcopenia and obesity. Sestrins, a family of proteins, play a crucial role in cellular adaption to stress and have shown potential in treating sarcopenia and obesity in pre-clinical models. Activation of sestrins can eliminate senescent cells and resolve inflammation. Therefore, sestrin activation, inflammation resolution, and senescent cell elimination may be a novel treatment strategy for age-associated sarcopenia and obesity.
AGEING RESEARCH REVIEWS
(2023)
Article
Cardiac & Cardiovascular Systems
Wei Feng Ma, Chani J. Hodonsky, Adam W. Turner, Doris Wong, Yipei Song, Jose Verdezoto Mosquera, Alexandra Ligay, Lotte Slenders, Christina Gancayco, Huize Pan, Nelson B. Barrientos, David Mai, Gabriel F. Alencar, Katherine Owsiany, Gary K. Owens, Muredach P. Reilly, Mingyao Li, Gerard Pasterkamp, Michal Mokry, Sander W. van der Laan, Bohdan B. Khomtchouk, Clint L. Miller
Summary: This study developed a workflow for single-cell RNA sequencing (scRNA-seq) analysis in atherosclerosis research and applied it to investigate a previously published human coronary single-cell dataset. The study revealed different derivations of fibroblast-like cells from smooth muscle cells in the atherosclerotic environment, and identified key changes in gene expression along their de-differentiation path. Additionally, the study highlighted potential mechanisms for several drugs in the atherosclerotic cellular environment. The development of the interactive web application PlaqView also allows non-expert scientists to explore and analyze relevant datasets.
Article
Biochemical Research Methods
Thomas Kenderdine, Reza Nemati, Andrew Baker, Martin Palmer, Jakub Ujma, Molly FitzGibbon, Limin Deng, Maksim Royzen, James Langridge, Daniele Fabris
JOURNAL OF MASS SPECTROMETRY
(2020)
Article
Multidisciplinary Sciences
Daniel Giovinazzo, Biljana Bursac, Juan Sbodio, Sumedha Nalluru, Thibaut Vignane, Adele M. Snowman, Lauren M. Albacarys, Thomas W. Sedlak, Roberta Torregrossa, Matthew Whiteman, Milos R. Filipovic, Solomon H. Snyder, Bindu D. Paul
Summary: Alzheimer's disease is characterized by memory, executive, and motor function deterioration, with neuropathological hallmarks including neurofibrillary tangles, paired helical filaments, and amyloid plaques. Dysregulation of signaling by hydrogen sulfide has been shown during aging and may play a role in preventing hyperphosphorylation of Tau in Alzheimer's disease. Furthermore, administering the H2S donor sodium GYY4137 to mouse models of Alzheimer's disease has been shown to ameliorate motor and cognitive deficits associated with the disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Chemistry, Inorganic & Nuclear
Kiwon Ok, Milos R. Filipovic, Sarah L. J. Michel
Summary: ZF proteins, including the emerging CCCH class, are widespread eukaryotic proteins that play crucial roles in gene regulation, with a particular focus on tristetraprolin (TTP). Various methods have been explored to modulate TTP activity, including targeting with exogenous metals and endogenous signaling molecules. Research has been conducted on using metals such as Fe, Cu, Cd, Au, and H2S to target TTP, with attention to new methods for monitoring metal exchange and RNA binding.
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2021)
Review
Geriatrics & Gerontology
Dunja Petrovic, Emilia Kouroussis, Thibaut Vignane, Milos R. Filipovic
Summary: Protein persulfidation plays an important role in physiology and pathophysiology, but its study is hampered by its instability and chemical reactivity. Persulfide levels are crucial for defense against oxidative damage, but decrease with age, leading to increased protein susceptibility to oxidation. Dysregulation of persulfidation is observed in various neurodegenerative diseases, suggesting potential for targeted drug design through further research in this area.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Monica Sueiro-Olivares, Jennifer Scott, Sara Gago, Dunja Petrovic, Emilia Kouroussis, Jasmina Zivanovic, Yidong Yu, Marlene Strobel, Cristina Cunha, Darren Thomson, Rachael Fortune-Grant, Sina Thusek, Paul Bowyer, Andreas Beilhack, Agostinho Carvalho, Elaine Bignell, Milos R. Filipovic, Jorge Amich
Summary: Persulfidation modification plays a crucial role in invasive pulmonary aspergillosis, influencing fungal virulence and host defense. A single nucleotide polymorphism in the human gene leads to reduced cellular persulfidation levels and a higher risk of invasive pulmonary aspergillosis in hematopoietic stem cell transplant recipients.
Review
Biochemistry & Molecular Biology
Maurits Roorda, Jan Lj Miljkovic, Harry van Goor, Robert H. Henning, Hjalmar R. Bouma
Summary: Hydrogen sulfide (H2S), previously known as a putrid, toxic gas, is now recognized as the third gasotransmitter alongside nitric oxide (NO) and carbon monoxide (CO) in mammalian systems. H2S plays a critical role in regulating vascular and cellular function in the kidneys, impacting blood flow, glomerular filtration rate, and the renin-angiotensin axis. Furthermore, H2S offers protection against acute kidney injury (AKI) by enhancing ATP production and preventing damage due to free radicals during hypoxia or ischemia-reperfusion injury. Understanding the (sub)cellular signaling mechanisms of H2S can lead to novel therapeutic targets for preventing kidney injury.
Meeting Abstract
Biochemistry & Molecular Biology
Milos R. Filipovic
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Stephanie K. Stoeckl, Roberto de Col, Milos R. Filipovic, Karl Messlinger
Summary: The study aimed to clarify the role of TRPA1 agonists in meningeal nociception, and the results suggest that TRPA1 activation may have an inhibitory function in the nociceptive trigeminal system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Cyril Statzer, Jin Meng, Richard Venz, Monet Bland, Stacey Robida-Stubbs, Krina Patel, Dunja Petrovic, Raffaella Emsley, Pengpeng Liu, Ianessa Morantte, Cole Haynes, William B. Mair, Alban Longchamp, Milos R. Filipovic, T. Keith Blackwell, Collin Y. Ewald
Summary: Inhibition of mTORC1 or translation increases ATF-4 expression independently of ISR signaling in C. elegans. ATF-4 promotes longevity by increasing hydrogen sulfide production through the enzyme CTH-2.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Multidisciplinary Sciences
Willem H. Koppenol
NATURE COMMUNICATIONS
(2022)
Editorial Material
Geriatrics & Gerontology
Bindu Diana Paul, Milos R. Filipovic
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Biology
Dylan Gerard Ryan, Ming Yang, Hiran A. Prag, Giovanny Rodriguez Blanco, Efterpi Nikitopoulou, Marc Segarra-Mondejar, Christopher A. Powell, Tim Young, Nils Burger, Jan Lj Miljkovic, Michal Minczuk, Michael P. Murphy, Alex von Kriegsheim, Christian Frezza
Summary: This study comprehensively evaluated the consequences of TCA cycle inhibition in murine kidney epithelial cells, revealing a rewiring of redox and amino acid metabolism dependent on ATF4 and ISR activation. Additionally, a divergent metabolic response was uncovered, where acute FHi but not SDHi could maintain asparagine levels through reductive carboxylation and cytosolic aspartate synthesis. The interplay between the TCA cycle, redox biology, and amino acid homeostasis was highlighted in this work.
Article
Multidisciplinary Sciences
Lavanya Moparthi, Viktor Sinica, Vamsi K. Moparthi, Mohamed Kreir, Thibaut Vignane, Milos R. Filipovic, Viktorie Vlachova, Peter M. Zygmunt
Summary: The temperature gating of hTRPA1 is regulated by the N-ARD and VSLD domains. The CTD is involved in cold sensitivity, while the VSLD is involved in heat sensitivity, and they are allosterically coupled to the channel pore gating.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Helmut Sies, Willem H. Koppenol
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Chemistry, Inorganic & Nuclear
Willem H. Koppenol
Summary: According to the literature, the Fenton reaction produces hydroxyl radicals at low pH and 25 degrees C. The formation of oxidoiron(2+), FeO2+, and its corresponding Gibbs energy of formation can be estimated based on observed reactions and assumptions. The presence of HCO3- leads to the formation of FeCO3(aq) and may facilitate the formation of the intermediate [FeHOO](+).
DALTON TRANSACTIONS
(2022)
