Article
Chemistry, Physical
Sylvain F. Royer, Xuan Gao, Robin R. Groleau, Marc W. van der Kamp, Steven D. Bull, Michael J. Danson, Susan J. Crennell
Summary: This study describes the creation of two new mutants of a hyperthermophilic aldolase, which can catalyze aldol reactions between pyruvate and non-natural L-glyceraldehyde to enable the diastereoselective synthesis of L-KDGlc and L-KDGal. High-resolution crystal structures of these mutant aldolases have been analyzed to understand the molecular mechanism underlying the changes in diastereoselectivities observed in these variant-catalyzed aldol reactions.
Article
Chemistry, Physical
Wenlong Zheng, Haoran Yu, Sai Fang, Kaitong Chen, Zhe Wang, Xiuli Cheng, Gang Xu, Lirong Yang, Jianping Wu
Summary: The study utilized a CAST/ISM strategy to accelerate the directed evolution of LTA by focusing on amino acid residues within the substrate pocket. Significant progress was made in the synthesis of products, showing promise for industrial applications.
Article
Biochemistry & Molecular Biology
Lei Gong, Guochao Xu, Xudong Cao, Ruizhi Han, Jinjun Dong, Ye Ni
Summary: A rapid and reliable method for determining aldol condensation activity of threonine aldolases (TAs) has been developed, allowing for high-throughput screening and characterization. Two beneficial variants of AxDTA were identified with increased aldol activity towards MSB. The DNPH method facilitated easy characterization of substrate specificity of AxDTA and variants towards aldehydes with different substituents, ultimately providing a new approach for preparing chiral beta-hydroxy-alpha-amino acids.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jonathan Extance, Michael J. Danson, Susan J. Crennell
Article
Biochemical Research Methods
Lisa Buddrus, Emma S. V. Andrews, David J. Leak, Michael J. Danson, Vickery L. Arcus, Susan J. Crennell
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Viatcheslav Zaitsev, Ulrike Johnsen, Matthias Reher, Marius Ortjohann, Garry L. Taylor, Michael J. Danson, Peter Schoenheit, Susan J. Crennell
Article
Biochemistry & Molecular Biology
Anna Aronsson, Fatma Guler, Maxim V. Petoukhov, Susan J. Crennell, Dmitri I. Svergun, Javier A. Linares-Pasten, Eva Nordberg Karlsson
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2018)
Article
Biochemistry & Molecular Biology
Mareike G. Posner, Abhishek Upadhyay, Rieko Ishima, Antreas C. Kalli, Gemma Harris, Joachim Kremerskothen, Mark S. P. Sansom, Susan J. Crennell, Stefan Bagby
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Biochemical Research Methods
Lisa Buddrus, Emma S. V. Andrews, David J. Leak, Michael J. Danson, Vickery L. Arcus, Susan J. Crennell
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2018)
Article
Biochemical Research Methods
Jonathan Extance, Susan J. Crennell, Kirstin Eley, Roger Cripps, David W. Hough, Michael J. Danson
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Nia L. Marrott, Jacqueline J. T. Marshall, Dmitri I. Svergun, Susan J. Crennell, David W. Hough, Jean M. H. van den Elsen, Michael J. Danson
BIOCHEMICAL JOURNAL
(2014)
Article
Chemistry, Medicinal
Zexin Chen, Patricia Marce, Ricardo Resende, Pedro M. Alzari, A. Carlos Frasch, Jean M. H. van den Elsen, Susan J. Crennell, Andrew G. Watts
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Stephen A. Wells, Susan J. Crennell, Michael J. Danson
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2014)
Article
Biochemical Research Methods
Giannina Espina, Kirstin Eley, Guillaume Pompidor, Thomas R. Schneider, Susan J. Crennell, Michael J. Danson
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2014)
Article
Biology
Alex Macpherson, Maisem Laabei, Zainab Ahdash, Melissa A. Graewert, James R. Birtley, Monika-Sarah E. D. Schulze, Susan Crennell, Sarah A. Robinson, Ben Holmes, Vladas Oleinikovas, Per H. Nilsson, James Snowden, Victoria Ellis, Tom Eirik Mollnes, Charlotte M. Deane, Dmitri Svergun, Alastair D. G. Lawson, Jean M. H. van den Elsen
Summary: This study investigates the binding of four knob domain peptides with complement C5 and reveals their effects on the target. The research identifies a new allosteric mechanism for inhibiting C5 and expands the therapeutic scope of C5. Additionally, it introduces knob domains as new low molecular weight antibody fragments with therapeutic potential.
Article
Immunology
Ayla A. Wahid, Rhys W. Dunphy, Alex Macpherson, Beth G. Gibson, Liudmila Kulik, Kevin Whale, Catherine Back, Thomas M. Hallam, Bayan Alkhawaja, Rebecca L. Martin, Ingrid Meschede, Maisem Laabei, Alastair D. G. Lawson, V. Michael Holers, Andrew G. Watts, Susan J. Crennell, Claire L. Harris, Kevin J. Marchbank, Jean M. H. van den Elsen
Summary: The cleavage of C3 to C3a and C3b leads to the formation of C3b dimers, which can modulate B cell activation and influence tolerogenic pathways. Understanding the functions of C3d(g) dimers could enhance our knowledge of complement in the fluid phase and potentially inform the development of novel therapies for immune system disorders in the future.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Rhys W. Dunphy, Ayla A. Wahid, Catherine R. Back, Rebecca L. Martin, Andrew G. Watts, Charlotte A. Dodson, Susan J. Crennell, Jean M. H. van den Elsen
Summary: This study reveals a new mechanism by which Staphylococcus aureus modulates B-cell activation using dimeric C3d, and provides detailed descriptions of the structural characteristics and functional mechanisms of this mechanism.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Physical
Sylvain F. Royer, Xuan Gao, Robin R. Groleau, Marc W. van der Kamp, Steven D. Bull, Michael J. Danson, Susan J. Crennell
Summary: This study describes the creation of two new mutants of a hyperthermophilic aldolase, which can catalyze aldol reactions between pyruvate and non-natural L-glyceraldehyde to enable the diastereoselective synthesis of L-KDGlc and L-KDGal. High-resolution crystal structures of these mutant aldolases have been analyzed to understand the molecular mechanism underlying the changes in diastereoselectivities observed in these variant-catalyzed aldol reactions.