Article
Immunology
Wei Wang, Jiatiao Jiang, Hao Chen, Yuanxing Zhang, Qin Liu
Summary: This study highlights the importance of FtsH in maintaining protein secretion homeostasis in Edwardsiella piscicida, as well as its crucial role in the virulence and proliferation of the pathogen within host cells. Deletion of FtsH significantly attenuated bacterial virulence, leading to reduced mortality rates and bacterial colonization in infected zebrafish. These findings indicate a close link between protein secretion homeostasis and bacterial virulence, providing valuable insights for further research in this field.
MICROBIAL PATHOGENESIS
(2021)
Review
Biochemistry & Molecular Biology
Karolina Szczepanowska, Aleksandra Trifunovic
Summary: Mitochondria rely on chaperones and proteases for protein homeostasis, with proteases like LON and ClpXP modulating essential functions beyond quality control. Impaired mitochondrial protease function is linked to various pathological conditions, and recent research has identified them as potential targets for anticancer therapies.
Article
Biochemistry & Molecular Biology
Maryam Kohram, Harsh Vashistha, Stanislas Leibler, BingKan Xue, Hanna Salman
Summary: This study takes an agnostic approach to analyzing bacterial growth and division, revealing dependencies that point to potentially new cell size control mechanisms. The research shows that cells born smaller tend to grow faster and sister cells exhibit correlations beyond traditional models.
Review
Cell Biology
Csaba Szabo
Summary: H2S, previously known as a toxic gas and environmental hazard, has been found to play multiple biological regulatory roles in mammalian cells as an endogenously produced gaseous transmitter. Its effects in mitochondria serve as an electron transport stimulator in physiological concentrations, particularly significant in cancer cells where it helps maintain mitochondrial organization and DNA repair.
Review
Genetics & Heredity
Krystie Chew, Linlin Zhao
Summary: Mitochondria play crucial roles in eukaryotic cells, carrying their own genomic DNA that encodes essential components of the oxidative phosphorylation system. The mitochondrial transcription factor TFAM interacts with different forms of DNA structures, impacting mtDNA repair and packaging. Understanding these interactions is important for maintaining cellular and organismal functions.
Article
Biochemistry & Molecular Biology
Inayathulla Mohammed, Kai A. Schmitz, Niko Schenck, Dimitrios Balasopoulos, Annika Topitsch, Timm Maier, Jan Pieter Abrahams
Summary: The mitochondrial Lon protease, LonP1, plays a crucial role in maintaining mitochondrial health by removing redundant proteins from the mitochondrial matrix. Cryo-EM analysis revealed eight nucleotide-dependent conformational states of LonP1. The study also showed how sequential ATP hydrolysis controls the translocation of substrate proteins in a 6-fold binding change mechanism.
Review
Microbiology
Thomas C. McLean, Tung B. K. Le
Summary: Segregation of genetic material is facilitated by the ParA-ParB-parS system in bacteria, in which ParB binds to parS and interacts with ParA to move the DNA cargo to daughter cells. The recent discovery of CTP-dependent cycling of ParB on bacterial chromosome has provided new insights into the molecular mechanism of the ParABS system and opened up potential avenues for future research and application beyond bacterial chromosome segregation.
CURRENT OPINION IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Christopher Jakubke, Rodaria Roussou, Andreas Maiser, Christina Schug, Felix Thoma, David Bunk, David Horl, Heinrich Leonhardt, Peter Walter, Till Klecker, Christof Osman
Summary: The study shows that yeast can distinguish between functional and defective mtDNA, promoting the generation of healthier daughter cells. Purifying selection for functional mtDNA occurs in a continuous mitochondrial network, depending on intact cristae morphology.
Article
Multidisciplinary Sciences
Yixiong Wang, Lubna Yasmin, Lei Li, Pinzhang Gao, Xia Xu, Xuejun Sun, Roseline Godbout
Summary: Researchers have discovered that the DEAD box protein DDX1 forms large aggregates in the cytoplasm of early mouse embryos and is involved in the formation of Membrane Associated RNA-containing Vesicles. Loss of Ddx1 leads to embryonic lethality and affects mitochondria function, as well as the spatial distribution of calcium in embryos. Furthermore, DDX1 plays a crucial role in regulating developmental and mitochondrial processes in embryos.
NATURE COMMUNICATIONS
(2022)
Review
Cardiac & Cardiovascular Systems
Haiying Wang, Yijun Han, Shenwei Li, Yunan Chen, Yafen Chen, Jing Wang, Yuqing Zhang, Yawen Zhang, Jingsuo Wang, Yong Xia, Jinxiang Yuan
Summary: This article introduces the structure and function of mitochondria, as well as various types of MDS associated with cardiac diseases.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Microbiology
Steven D. Goodman, Lauren O. Bakaletz
Summary: Bacterial biofilms play a significant role in antibiotic resistance, pathogenesis, chronic disease, and recurring bacterial infections. The extracellular DNA (eDNA)-dependent matrix is crucial for the stability and survival of biofilms by protecting resident bacteria and facilitating interactions with other bacteria. Targeting the DNABII proteins and eDNA has shown promising results in resolving biofilms.
Article
Cell Biology
Bin Wang, Haixia Shi, Bo Yang, Zhigang Miao, Miao Sun, Hao Yang, Xingshun Xu
Summary: This study found that in the brains of depressed mice and Ahi1 knockout mice, ATP levels decreased while mtDNA copy numbers increased. Two proteins, Ahi1 and glucocorticoid receptor (GR), which are related to stress and depressive behaviors, were significantly decreased. Regular exercise is shown to increase Ahi1/GR complex levels and improve depressive behaviors.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Xueqiao Wang, Lin Zhu, Zhuying Wei, Mingjuan Gu, Miaomiao Yang, Xinyu Zhou, Chunling Bai, Guanghua Su, Xuefei Liu, Lei Yang, Guangpeng Li
Summary: This study investigated the effects of exogenous fat-1 transgene on energy metabolism and its relationship with DNA methylation. The results showed that exogenous fat-1 transgene significantly increased n-3 PUFA content, while decreasing n-6 PUFA content and the n-6: n-3 PUFA ratio. Furthermore, the increase in exogenous fat-1 transgene reduced ATP synthesis and the activity and expression of key rate-limiting enzymes in glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation. DNA methylation sequencing indicated that the inhibition of gene expression may be due to altered DNA methylation. Thus, altering the balance of n-6/n-3 PUFA could regulate DNA methylation, which in turn affects mitochondrial energy metabolism.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Cell Biology
Corinne Bischof, Peter Mirtschink, Ting Yuan, Meiqian Wu, Chaonan Zhu, Jaskiran Kaur, Minh Duc Pham, Suam Gonzalez-Gonoggia, Marie Hammer, Eva-Maria Rogg, Rahul Sharma, Katharina Bottermann, Bettina Gercken, Eman Hagag, Corinne Berthonneche, Samuel Sossalla, Sebastian N. Stehr, Joachim Maxeiner, Maria Anna Duda, Mathieu Latreille, Nicola Zamboni, Fabio Martelli, Thierry Pedrazzini, Stefanie Dimmeler, Jaya Krishnan
Summary: Endoreplication, a type of growth mechanism in disease, is still poorly understood in terms of its metabolic basis and connection to morphologic growth. Studies have shown a direct coupling between endoreplication and hypertrophy in heart disease, revealing a new mechanism for pathologic overgrowth in failing myocardium.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Review
Immunology
Wolfgang Eisenreich, Thomas Rudel, Juergen Heesemann, Werner Goebel
Summary: Persistence is a potent survival strategy common to almost all bacteria, allowing a subpopulation to withstand adverse conditions and revive once the stress is removed. Various factors and pathways contribute to the formation of persistence, even in stress-free bacterial populations, with growth potentially induced by stress conditions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jacob A. Bauer, L'ubomir Borko, Jelena Pavlovic, Eva Kutejova, Vladena Bauerova-Hlinkova
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2020)
Article
Biochemistry & Molecular Biology
Zuzana Pevalova, Vladimir Pevala, Nicholas J. Blunsom, Dana Tahotna, Veronika Kotrasova, Roman Holic, Lucia Pokorna, Jacob A. Bauer, Eva Kutejova, Shamshad Cockcroft, Peter Griac
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2019)
Review
Biochemistry & Molecular Biology
Veronika Vozarikova, Nina Kunova, Jacob A. Bauer, Jan Frankovsky, Veronika Kotrasova, Katarina Prochazkova, Vladimira Dzugasova, Eva Kutejova, Vladimir Pevala, Jozef Nosek, Lubomir Tomaska
Review
Biology
Veronika Kotrasova, Barbora Keresztesova, Gabriela Ondrovicova, Jacob A. Bauer, Henrieta Havalova, Vladimir Pevala, Eva Kutejova, Nina Kunova
Summary: Mitochondria play a major role in providing energy to cells and responding to stress factors and metabolic changes. Post-translational modification, such as phosphorylation, is a fast and efficient way for cells to adapt to changing conditions, but it also alters protein structure and function. Kinases, responsible for phosphorylation, have a significant impact on mitochondrial biogenesis and function under different cellular conditions.
Review
Biochemistry & Molecular Biology
Henrieta Havalova, Gabriela Ondrovicova, Barbora Keresztesova, Jacob A. Bauer, Vladimir Pevala, Eva Kutejova, Nina Kunova
Summary: Heat shock proteins (HSPs) are found in all domains of life, particularly in mitochondria where they play crucial roles in maintaining organelle function and health. The human mitochondrial HSP70 chaperone system consists of mtHSP70 or mortalin and three co-chaperones, which work together to stabilize and import nuclear gene products and regulate protein folding functions. Dysregulation of these molecular chaperones is often linked to neurological disorders, genetic diseases, and cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Nina Kunova, Henrieta Havalova, Gabriela Ondrovicova, Barbora Stojkovicova, Jacob A. Bauer, Vladena Bauerova-Hlinkova, Vladimir Pevala, Eva Kutejova
Summary: Mitochondrial proteins are transported into mitochondria using a sophisticated system, and their proper folding and assembly rely on the cleavage of targeting presequences. Mitochondrial processing peptidases play a crucial role in mitochondrial homeostasis and their malfunctions are linked to severe human diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jan Frankovsky, Barbora Keresztesova, Jana Bellova, Nina Kunova, Nikola Canigova, Katerina Hanakova, Jacob A. Bauer, Gabriela Ondrovicova, Veronika Lukacova, Barbara Sivakova, Zbynek Zdrahal, Vladimir Pevala, Katarina Prochazkova, Jozef Nosek, Peter Barath, Eva Kutejova, Lubomir Tomaska
Summary: Acylation modifications, such as lysine succinylation, are important in regulating protein activity, with some occurring non-enzymatically due to an increase in acyl group donors. In eukaryotes, mitochondrial proteins are predominantly affected by lysine succinylation, as succinate donors are primarily generated in the tricarboxylic acid cycle. This modification can have a profound effect on protein function, as seen in the succinylation of Abf2p affecting its DNA-binding activity and sensitivity to protease digestion.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Dominik Stastny, Livia Petriskova, Dana Tahotna, Jacob Bauer, Lucia Pokorna, Roman Holic, Martin Valachovic, Vladimir Pevala, Shamshad Cockcroft, Peter Griac
Summary: Yeast Sec14-like phosphatidylinositol transfer proteins (PITPs) have a hydrophobic cavity capable of binding and transferring lanosterol. This is the first identification of lanosterol transfer proteins. A double mutant lacking Pdr16p and Pdr17p showed increased cellular lanosterol levels, indicating the importance of PI-lanosterol transfer proteins in sterol biosynthesis.
Article
Immunology
Klaudia Meskova, Katarina Martonova, Patricia Hrasnova, Kristina Sinska, Michaela Skrabanova, Lubica Fialova, Stefana Njemoga, Ondrej Cehlar, Olga Parmar, Petr Kolenko, Vladimir Pevala, Rostislav Skrabana
Summary: Laboratory production of recombinant mammalian proteins, particularly antibodies, can be achieved using transient gene expression in suspension-adapted Chinese Hamster Ovary (CHO) cell lines. This method allows for secretion of antibodies into the media and can be glycosylated. However, protein production in CHO cells can be costly and variable. Optimization can be done in aspects such as gene delivery, cultivation process, and expression plasmid design. In this study, a TGE platform for medium-scale production of antibodies and other proteins in CHO cells was proposed, using a new expression vector and recyclable labware to reduce production cost. The platform's potential was demonstrated through the production and crystallization of antibody Fabs and their complexes.
Meeting Abstract
Chemistry, Multidisciplinary
Jacob Bauer, Jelena Pavlovic, Eva Kutejova, Vladena Bauerova
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES
(2019)
