Article
Plant Sciences
Zhoubin Liu, Jingshuang Song, Wu Miao, Bozhi Yang, Zhuqing Zhang, Wenchao Chen, Fangjun Tan, Huan Suo, Xiongze Dai, Xuexiao Zou, Lijun Ou
Summary: The study identified the proteome and acetylome of two pepper varieties with different levels of cold resistance. Differentially expressed proteins and acetylated proteins were discovered, revealing dynamic changes in key carbon assimilation enzymes under low-temperature stress. This research expands the catalog of lysine acetylation substrates and sites in Solanaceae crops, providing new insights for posttranslational modification studies.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Muhammad Ahsan Altaf, Huangying Shu, Yuanyuan Hao, Muhammad Ali Mumtaz, Xu Lu, Zhiwei Wang
Summary: Melatonin treatment improves photosynthetic performance in plants under cold stress by enhancing gas exchange characteristics and photosynthetic pigment content. It also upregulates the expression of biosynthetic genes and increases the activity of photosynthetic enzymes. Melatonin treatment mitigates the negative effects of cold stress on photosystem activity and maintains photosynthetic efficiency.
Article
Chemistry, Multidisciplinary
Wu Miao, Jingshuang Song, Yanwu Huang, Rongyun Liu, Gaofeng Zou, Lijun Ou, Zhoubin Liu
Summary: This study investigated the physiological and transcriptome responses of cold-tolerance and cold-sensitive pepper lines under cold-rewarm treatments. Compared with the sensitive line, the tolerant line exhibited better resilience and differential expression of genes related to photosynthesis, plant hormone signal transduction, and DNA damage repair pathways. Additionally, NAC transcription factors and hub genes such as zinc finger proteins and heat shock proteins were identified as potential key factors in the response to cold stress and cold tolerance in pepper.
APPLIED SCIENCES-BASEL
(2021)
Article
Plant Sciences
Victoria Florencio-Ortiz, Susana Selles-Marchart, Jose L. Casas
Summary: Aphid attacks trigger plant defense responses and lead to changes in the proteome of pepper leaves, with low density infestation resulting in a smaller number of differentially expressed proteins compared to high density infestation. The altered proteins are involved in various aspects of plant primary metabolism, with photosynthesis being the most affected process.
Article
Multidisciplinary Sciences
Shi-Lin Tian, Abid Khan, Wen-Na Zheng, Li Song, Jun-He Liu, Xiao-Qian Wang, Li Li
Summary: The long-term use of chemical fertilizers has caused problems in farmland, leading to the search for new bio-fertilizers in agriculture. This study found that Chlorella extract can significantly improve the growth of pepper seedlings, potentially reducing reliance on chemical fertilizers.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Jianwei Zhang, Le Liang, Yongdong Xie, Zhao Zhao, Lihong Su, Yi Tang, Bo Sun, Yunsong Lai, Huanxiu Li
Summary: This study investigated the molecular mechanisms behind peppers' response to cold stress using transcriptomic and metabolomic analyses. The results revealed that the content of free polyamines, plant hormones, and osmolytes increased in response to cold stress, and the ICE-CBF-COR pathway played a crucial role in regulating peppers' responses to cold stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Ali Raza, Wei Su, Muhammad Azhar Hussain, Sundas Saher Mehmood, Xuekun Zhang, Yong Cheng, Xiling Zou, Yan Lv
Summary: Rapeseed is an important oilseed crop and enhancing cold stress tolerance is crucial for agricultural investigation and crop improvement. This study identified stress-responsive genes, metabolites, and pathways in cold-tolerant and cold-sensitive rapeseed varieties. Functional analysis revealed the significance of starch and sucrose metabolism and phenylalanine metabolism in cold stress adaption. Six candidate genes were selected for controlling adaption to low temperature and showed significant differences in freezing tolerance.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jianwei Zhang, Minghui Xie, Guofeng Yu, Dong Wang, Zeping Xu, Le Liang, Jiachang Xiao, Yongdong Xie, Yi Tang, Guochao Sun, Bo Sun, Zhi Huang, Yunsong Lai, Huanxiu Li
Summary: In this study, a SPDS gene named CaSPDS was identified and cloned from pepper. It was found to be highly expressed in pepper and was shown to play important roles in cold stress response in both pepper and Arabidopsis. It can enhance the cold tolerance of pepper.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Mian Abdur Rehman Arif, Pasquale Tripodi, Muhammad Qandeel Waheed, Irfan Afzal, Sibylle Pistrick, Gudrun Schuetze, Andreas Boerner
Summary: Seed longevity is an important trait in genebank management, and little is known about the genetic basis of this trait in Capsicum. In this study, 1152 Capsicum accessions stored for 40 years were evaluated for seed longevity using SNP markers. A total of 224 marker trait associations were identified, and several candidate genes were discussed.
Article
Plant Sciences
Xiaodi Wang, Ning Li, Tianxiang Zan, Kai Xu, Shenghua Gao, Yanxu Yin, Minghua Yao, Fei Wang
Summary: This study systematically analyzed the structure, conserved motifs, and expression patterns of the CaTIFY gene family in pepper. The results showed that CaTIFY genes are involved in plant stress responses, with CaTIFY7 and CaTIFY10b being significantly induced by cold stress. Overexpression of CaTIFY7 and CaTIFY10b enhanced cold tolerance in plants.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Cengiz Kaya, Sergey Shabala
Summary: This study investigated the mechanisms by which melatonin (MT) improves drought tolerance in pepper plants. The plants were treated with a foliar spray of 0.1 mM MT and grown under 80% and 40% field capacity for 3 days. Drought stress significantly decreased plant dry weight, water content, PSII efficiency, chlorophyll, protein, and nitrogen content, while increasing oxidative stress markers and enzyme activities. MT pre-treatment alleviated oxidative stress and improved nitrogen metabolism by activating various enzymes. It also reduced nitrogen content and enhanced plant growth and photosynthesis.
FUNCTIONAL PLANT BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Fengkun Lu, Wenjing Duan, Yue Cui, Junwei Zhang, Dong Zhu, Ming Zhang, Yueming Yan
Summary: This study evaluated the drought tolerance of the wheat Zhongmai 8601-Thinopyrum intermedium 7XL/7DS translocation line YW642 under drought stress and conducted a proteome analysis to identify drought-resistant proteins in the developing grains. The results showed that YW642 had better drought tolerance compared to Zhongmai 8601. The analysis identified multiple proteins involved in stress/defense, energy metabolism, and protein metabolism/folding that were upregulated in YW642 under drought stress. The presence of stress-related elements in the promoter regions of these drought-responsive protein genes suggests their important roles in drought defense. These findings provide valuable resources for improving wheat drought tolerance.
Article
Agronomy
Jieun Seo, Jeong Gu Lee, Byoung-Cheorl Kang, Sooyeon Lim, Eun Jin Lee
Summary: This study investigated the relationship between hormone levels, gene expression, and chilling injury in pepper fruit. 'TK' genotype was less sensitive to low temperature, while 'GG' genotype showed higher sensitivity, and GABA and CaARF19 were possibly associated with chilling injury levels.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jianwei Zhang, Le Liang, Jiachang Xiao, Yongdong Xie, Li Zhu, Xinru Xue, Linyu Xu, Peihan Zhou, Jianzhao Ran, Zhi Huang, Guochao Sun, Yunsong Lai, Bo Sun, Yi Tang, Huanxiu Li
Summary: In this study, six PAO genes were identified in the pepper genome, and it was found that CaPAO2 and CaPAO4 were significantly upregulated under cold stress. Further experiments demonstrated that overexpression of CaPAO2 and CaPAO4 enhanced freezing-stress tolerance in plants. These findings provide candidate genes for breeding cold-stress-tolerant transgenic pepper cultivars.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Horticulture
Brandon Estefano Morales-Merida, Jesus Christian Grimaldi-Olivas, Abraham Cruz-Mendivil, Claudia Villicana, Jose Benigno Valdez-Torres, Jose Basilio Heredia, Ruben Leon-Chan, Luis Alberto Lightbourn-Rojas, Josefina Leon-Felix
Summary: Ultraviolet-B radiation and cold stress restrict plant growth and development, leading to changes in gene expression. This study identified hub genes through RNA-Seq data and WGCNA analyses. A total of 25, 24, and 29 transcription factors were found at different time points under combined stress. RVE1, COL2, and DBB1b were identified as candidate hub genes. Furthermore, GO and KEGG enrichment analysis revealed their involvement in energy production, antioxidant system, signaling pathways, light response, and cellular homeostasis. These findings lay the foundation for further investigation into plant responses to UV-B radiation and cold stress.
Article
Plant Sciences
Morteza Soleimani Aghdam, Soumya Mukherjee, Francisco Borja Flores, Marino B. Arnao, Zisheng Luo, Francisco J. Corpas
Summary: Melatonin has important effects on plant growth and development, with antioxidant properties and interactions with plant regulators and reactive oxygen and nitrogen species. Its application in horticultural crops can alleviate stress, delay senescence, and preserve product quality, which is of significant economic importance.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Irene Romero, M. Isabel Escribano, Carmen Merodio, M. Teresa Sanchez-Ballesta
Summary: This article discusses the application of short-term high-CO2 treatments and multi-omics approach to maintain the postharvest quality of soft fruits. It also highlights the importance of high-throughput transcriptomic technologies in understanding the mechanisms modulated by short-term gaseous treatments.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
J. Pastor-Fernandez, P. Sanchez-Bel, J. Gamir, V. Pastor, N. Sanmartin, M. Cerezo, S. Andres-Moreno, V. Flors
Summary: The research explores the molecular mechanisms of Systemin-induced resistance against Plectosphaerella cucumerina in Arabidopsis plants, revealing the activation of protein complexes and signaling events by Systemin, as well as the involvement of phenolic and indolic compounds in Sys-IR.
Review
Food Science & Technology
Silvia Leticia Rivero Meza, Adriano Hirsch Ramos, Lazaro Canizares, Chirle de Oliveria Raphaelli, Betina Bueno Peres, Cesar Augusto Gaioso, Isabel Egea, Yanira Estrada, Francisco Borja Flores, Mauricio de Oliveira
Summary: Amaranth grains are a rich source of proteins with a well-balanced amino acid profile. They have high bioavailability and can be a potential substitute for animal-based foods. Consumption of amaranth proteins can promote human health by reducing the risk of chronic diseases and their bioactive peptides have antioxidant, anti-inflammatory, anti-hypertensive and antimicrobial activities.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Irene Romero, Raquel Rosales, M. Isabel Escribano, Carmen Merodio, M. Teresa Sanchez-Ballesta
Summary: Short-term gaseous treatments can improve rachis quality during table grape postharvest by reducing oxidative stress and cell wall degradation. This improvement is achieved through the modulation of the non-enzymatic and enzymatic antioxidant systems, as well as the expression of ethylene and ABA regulatory genes. This study provides new insights into the mechanisms underlying the beneficial effects of gaseous treatments on rachis browning.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Patryk Frackowiak, Barbara Wrzesinska, Przemyslaw Wieczorek, Paloma Sanchez-Bel, Laura Kunz, Antje Dittmann, Aleksandra Obrepalska-Steplowska
Summary: This study investigated the effects of benzo(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) treatment on the defense and developmental processes in tomato plants. Multi-omics analyses revealed that BTH treatment significantly influenced the plant's immune response and activation of certain genes, as well as the accumulation of important phytohormones. The study also uncovered the involvement of BTH in the induction of thiamine metabolism in tomatoes. These findings provide valuable insights into the plant's defense mechanisms against virus infections.
Article
Plant Sciences
Ivan del Olmo, Irene Romero, Maria Dolores Alvarez, Rosa Tarradas, Maria Teresa Sanchez-Ballesta, Maria Isabel Escribano, Carmen Merodio
Summary: This study analyzed the transcriptomic changes of wild strawberries under different storage conditions using RNA-seq and identified key genes related to cell wall integrity, softening, and oxidative stress. High CO2 treatment increased firmness and maintained cell turgor, while air-cold storage and non-cold storage at ambient temperature led to gene transcription changes associated with softening and cell wall disassembly in strawberries.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Isabel Egea, Yanira Estrada, Francisco B. Flores, Maria C. Bolarin
Summary: Climate change and increasing world population have highlighted the importance of developing crops with enhanced abiotic stress tolerance and higher nutritional value. This review summarizes recent advances in the understanding of tomato's response to abiotic stress and its impact on fruit yield and quality. Key genes involved in abiotic stress tolerance and the accumulation of important metabolites in tomato fruit are discussed, along with the need for genetic diversity in breeding programs to improve these traits.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Review
Plant Sciences
Isabel Egea, Yanira Estrada, Celia Faura, Jose M. Egea-Fernandez, Maria C. Bolarin, Francisco B. Flores
Summary: Improved abiotic stress tolerance and nutritive value in food is crucial due to climate change and increasing population. Among these stresses, salt stress is a major problem in agriculture. In order to utilize marginal lands affected by salinity and low-quality saline water, exploring new sources of genetic variation like salt-tolerant alternative crops or underexploited crops is a promising approach.
FRONTIERS IN PLANT SCIENCE
(2023)
Editorial Material
Plant Sciences
Maria Teresa Sanchez-Ballesta, Itay Maoz, Carlos R. Figueroa
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Julio Salazar-Bermeo, Bryan Moreno-Chamba, Maria Concepcion Martinez-Madrid, Manuel Valero, Joaquin Rodrigo-Garcia, Farah Hosseinian, Francisco Martin-Bermudo, Manuel Aguado, Rosa de la Torre, Nuria Marti, Domingo Saura
Summary: Gas chromatography (GC) techniques were optimized to accurately identify cannabinoids in cannabis samples, as rapid conditions may lead to mislabeling. Moreover, liquid chromatography (HPLC-DAD) analysis of forensic samples showed higher CBD and THC content compared to GC-MS determination. This study highlights the importance of optimizing GC techniques to avoid mislabeling cannabinoids in cannabis samples.
Article
Biochemistry & Molecular Biology
Julio Salazar-Bermeo, Bryan Moreno-Chamba, Rosa Heredia-Hortiguela, Victoria Lizama, Maria Concepcion Martinez-Madrid, Domingo Saura, Manuel Valero, Madalina Neacsu, Nuria Marti
Summary: The use of green technologies such as ultrasound and natural deep eutectic solvents (NADES) for revalorisation of food and agricultural by-products represents a sustainable way to tackle waste and promote a healthier environment while delivering much-needed functional food ingredients for an increasingly unhealthy population.
Review
Microbiology
Julia Pastor-Fernandez, Paloma Sanchez-Bel, Victor Flors, Miguel Cerezo, Victoria Pastor
Summary: The plant immunity system is gaining more attention as new elements and roles are discovered. Phytocytokines are being recognized as a part of a compound family that can amplify the immune response. This review aims to highlight the role of phytocytokines in the complex plant perception and signaling events during the immune response to biotic stress.
Article
Plant Sciences
Yanira Estrada, Felix Plasencia, Ana Ortiz-Atienza, Celia Faura, Francisco B. Flores, Rafael Lozano, Isabel Egea
Summary: Climate change worsens abiotic stresses on crops, such as salinization, negatively affecting crop yield. Strategies to improve salt tolerance, like using salt-tolerant rootstocks, are crucial. The SlCBL10 gene was found to have a different function in tomato shoot and root. Silencing SlCBL10 in rootstocks improved salt tolerance and fruit yield by regulating Na+ and K+ homeostasis. SlCBL10 silencing induced expression of SlSOS1 and affected expression of LKT1 transporter and SlSKOR channel, leading to improved Na+ and K+ balance in the plant.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)
