Article
Chemistry, Multidisciplinary
Takayuki Katoh, Hiroaki Suga
Summary: The study demonstrates the feasibility of multiple and consecutive incorporations of alpha-aminoxy/alpha-hydrazino acids with different configurations into peptides during ribosomal translation, including macrocyclic peptide scaffolds.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Multidisciplinary Sciences
M. Tyler Nelson, Mark R. Charbonneau, Heidi G. Coia, Mary J. Castillo, Corey Holt, Eric S. Greenwood, Peter J. Robinson, Elaine A. Merrill, David Lubkowicz, Camilla A. Mauzy
Summary: Engineered bacteria, or synthetic biotics, utilize synthetic biology tools for therapeutic purposes and require translational testing to predict their function in the human body. Gut-on-a-chip microfluidics technology allows for characterization of strain function within a simulated human gastrointestinal tract, providing a framework to predict the function of candidate strains in vivo.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Ming Liu, Yiheng Huang, Hongwei Zhang, Dawn Aitken, Michael C. Nevitt, Jason S. Rockel, Jean-Pierre Pelletier, Cora E. Lewis, James Torner, Yoga Raja Rampersaud, Anthony V. Perruccio, Nizar N. Mahomed, Andrew Furey, Edward W. Randell, Proton Rahman, Guang Sun, Johanne Martel-Pelletier, Mohit Kapoor, Graeme Jones, David Felson, Dake Qi, Guangju Zhai
Summary: Obesity is associated with altered amino acid metabolism, and restricting branched-chain amino acids in a high-fat diet can prevent obesity and insulin resistance.
Article
Chemistry, Multidisciplinary
Om Shanker Tiwari, Ruth Aizen, Massimiliano Meli, Giorgio Colombo, Linda J. W. Shimon, Noam Tal, Ehud Gazit
Summary: Molecular self- and co-assembly have the ability to form diverse and well-defined supramolecular structures with notable physical properties. By exploring the co-assembly of L-His with different aromatic amino acids, including Phe, Tyr, and Trp, we expanded the structural space of amino acid nanomaterials. The best seamless co-assembly was observed between L-His and L-Phe, resulting in the formation of single crystals.
Article
Chemistry, Physical
Liuzhu Wang, Shiqing Diao, Yangyang Sun, Shuiqin Jiang, Yan Liu, Hualei Wang, Dongzhi Wei
Summary: A dual cofactor-dependent glutamate dehydrogenase was identified in this study to improve the cofactor utilization efficiency in whole-cell catalysis by changing its substrate specificity. By constructing a three-enzyme co-expression system and using high concentration of starting material, a high yield of product can be obtained efficiently.
CATALYSIS SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Sahabaj Mondal, Surajit Singh, Ananda Shit, Santosh Kumar, Debasish Haldar
Summary: The tryptophan-mediated photo cleavage of disulfide bonds and resulting defibrillation process has been demonstrated. A supramolecular fiber-like network structure is formed through the self-assembly of dibenzyloxycarbonyl-L-cystine by non-covalent interactions, resulting in a hydrogel. The hydrogel shows thixotropic properties and is responsive to heat and pH. However, UV irradiation leads to disulfide bond cleavage, causing defibrillation and a gel-to-sol transition. The fiber exhibits amyloid-like structure and positive results in the Congo red assay.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Immunology
Diana Martin, M. Camino Ordas, Ines Carvalho, Patricia Diaz-Rosales, Noelia Nunez-Ortiz, Samuel Vicente-Gil, Aitor Arrogante, Carlos Zarza, Marina Machado, Benjamin Costas, Carolina Tafalla
Summary: This study investigates the effects of methionine, tryptophan, and lysine on the functionality of rainbow trout IgM(+) B cells. The results show that methionine supplementation significantly increases cell proliferation and IgM secretion, while tryptophan and lysine have minor or even negative effects.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Nutrition & Dietetics
Maryam Hajishafiee, Sina S. Ullrich, Penelope Ce Fitzgerald, Michael Horowitz, Kylie Lange, Sally D. Poppitt, Christine Feinle-Bisset
Summary: In healthy men, intragastric tryptophan increases plasma cholecystokinin levels and effectively suppresses energy intake, leading to enhanced fullness after intake. Obese men showed less response to tryptophan compared to lean men.
JOURNAL OF NUTRITION
(2021)
Article
Nutrition & Dietetics
Maryam Hajishafiee, Sina S. Ullrich, Penelope Ce Fitzgerald, Michael Horowitz, Kylie Lange, Sally D. Poppitt, Christine Feinle-Bisset
Summary: Intragastric Trp has potent energy intake-suppressant effects in both lean men and those with obesity, apparently related to the Trp:LNAAs ratio. In addition, postmeal, fullness was greater after Trp-3 in men with obesity.
JOURNAL OF NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Martina Bortolami, Paola Di Matteo, Daniele Rocco, Marta Feroci, Rita Petrucci
Summary: Agropyron repens (couch grass) is a rhizomatous plant with worldwide distribution and pharmacological applications. This study focused on the chemical analysis of its essential oil and aqueous extracts, particularly the phenolic compounds. Using mass spectrometry and chromatography, the metabolic and phenolic profiles of Agropyron repens rhizome herbal tea were investigated, revealing the presence of several bioactive compounds. This study provides new insights for the potential applications of Agropyron repens rhizome.
Article
Chemistry, Inorganic & Nuclear
Yunxiao Ma, Mingchang Zhu, Ying Zhang, Enjun Gao, Shuangyan Wu
Summary: In this study, a novel self-calibrated luminescent sensor was developed for the detection of L-tryptophan (Trp) in plasma. The sensor exhibited excellent sensitivity, selectivity, rapid response time, and low detection limit. The mechanism of luminescence was mainly attributed to the energy transfer between Trp and the ligand, Sm3+ ions. Satisfactory recovery rates were obtained in plasma, indicating the potential application of the sensor for Trp detection.
INORGANICA CHIMICA ACTA
(2022)
Review
Immunology
Rebecca R. Crowther, Joseph E. Qualls
Summary: This article provides a summary of the current knowledge on anti-Mtb immunity, focusing on immune cell amino acid metabolism, specifically discussing the roles of L-arginine and L-tryptophan in TB patients. By uncovering the immune cell contribution during Mtb infection and how amino acid utilization regulates their functions, novel host-directed therapies may be developed and refined to help eradicate TB.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Shaymus Contorno, Richard E. Darienzo, Rina Tannenbaum
Summary: The study focused on utilizing Raman spectroscopy to analyze aromatic amino acids as a potential biomarker for breast cancer detection. Results showed that cancerous breast tissues and cells exhibit significant overexpression of aromatic amino acids, suggesting the possibility of using these Raman bands as a diagnostic tool for breast cancer.
SCIENTIFIC REPORTS
(2021)
Article
Endocrinology & Metabolism
Marja K. Puurunen, Jerry Vockley, Shawn L. Searle, Stephanie J. Sacharow, John A. Phillips, William S. Denney, Benjamin D. Goodlett, David A. Wagner, Larry Blankstein, Mary J. Castillo, Mark R. Charbonneau, Vincent M. Isabella, Vasu V. Sethuraman, Richard J. Riese, Caroline B. Kurtz, Aoife M. Brennan
Summary: The engineered E. coli strain SYNB1618 shows potential for the treatment of PKU, as it was safe and well tolerated in a phase 1/2a clinical trial with both healthy volunteers and PKU patients. The study demonstrated dose-responsive increases in strain-specific Phe metabolites in plasma and urine, supporting the proof of concept for using engineered bacteria in the treatment of rare metabolic disorders.
Article
Plant Sciences
Hafiza Iqra Almas, Zaib Un-Nisa, Sumera Anwar, Abida Kausar, Fozia Farhat, Muneeb Munawar, Raziah Khalizadieh
Summary: The foliar spray of 0.02% Met significantly improved growth and yield parameters under 6 dS/m stress condition. The combination of 0.02% Met + 0.02% Phe increased carotenoid contents, total carbohydrates, total free amino acids, proline contents, while reducing lipid peroxidation rate and electrolyte leakage compared to salt-treated plants alone. The foliar application of Met and Phe enhanced plasma membrane stability, osmolyte contents, and antioxidative enzyme activity in tomato plants, indicating that it effectively mitigated the harmful effects of salinity and increased tomato yield.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2021)
Article
Chemistry, Analytical
Belen Hernandez, Fernando Pflueger, Sergei G. Kruglik, Regis Cohen, Mahmoud Ghomi
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2015)
Article
Spectroscopy
Belen Hernandez, Yves-Marie Coic, Fernando Pflueger, Sergei G. Kruglik, Mahmoud Ghomi
JOURNAL OF RAMAN SPECTROSCOPY
(2016)
Article
Chemistry, Physical
Belen Hernandez, Eduardo Lopez-Tobar, Santiago Sanchez-Cortes, Yves-Marie Coic, Bruno Baron, Alexandre Chenal, Sergei G. Kruglik, Fernando Pfluger, Regis Cohen, Mahmoud Ghomi
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2016)
Article
Chemistry, Analytical
Belen Hernandez, Pascal Houze, Fernando Pfluger, Sergei G. Kruglik, Mahmoud Ghomi
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2017)
Article
Chemistry, Physical
Sergei G. Kruglik, Byung-Kuk Yoo, Jean-Christophe Lambry, Jean-Louis Martin, Michel Negrerie
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2017)
Article
Chemistry, Physical
Andrej Hovan, Shubhashis Datta, Sergei G. Kruglik, Daniel Jancura, Pavol Miskovsky, Gregor Bano
JOURNAL OF PHYSICAL CHEMISTRY B
(2018)
Article
Chemistry, Physical
Shubhashis Datta, Andrej Hovan, Annamaria Jutkova, Sergei G. Kruglik, Daniel Jancura, Pavol Miskovsky, Gregor Bano
JOURNAL OF PHYSICAL CHEMISTRY B
(2018)
Article
Biophysics
Aurelien Bour, Sergei G. Kruglik, Morgan Chabanon, Padmini Rangamani, Nicolas Puff, Stephanie Bonneau
BIOPHYSICAL JOURNAL
(2019)
Article
Chemistry, Physical
Randa Mrad, Sergei G. Kruglik, Nassim Ben Brahim, Rafik Ben Chaabane, Michel Negrerie
JOURNAL OF PHYSICAL CHEMISTRY C
(2019)
Article
Biochemical Research Methods
Anikitos Garofalakis, Sergei G. Kruglik, Tigran Mansuryan, Andre Gillibert, Luc Thiberville, Frederic Louradour, Christine Vever-Bizet, Genevieve Bourg-Heckly
JOURNAL OF BIOMEDICAL OPTICS
(2019)
Article
Chemistry, Multidisciplinary
Belen Henandez, Pauline Legrand, Sophie Dufay, Rabah Gahoual, Santiago Sanchez-Cortes, Sergei G. Kruglik, Jean-Roch Fabreguettes, Jean-Philippe Wolf, Pascal Houze, Mahmoud Ghomi
Article
Biophysics
Belen Hernandez, Jean-Marc Crowet, Joseph Thiery, Sergei G. Kruglik, Nicolas Belloy, Stephanie Baud, Manuel Dauchez, Laurent Debelle
BIOPHYSICAL JOURNAL
(2020)
Article
Biochemical Research Methods
Belen Hernandez, Fernando Pflueger, Sergei G. Kruglik, Mahmoud Ghomi
Summary: This article focuses on the structural dynamics of four trimers in an aqueous environment and evaluates the stability of their secondary structures using density functional theory calculations. The study demonstrates that the choice of functional affects the stability of intramolecular hydrogen bonds and conformational equilibrium in the trimers, while explicit hydration has a significant impact on the conformational landscapes of the peptides.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2021)
Review
Biochemical Research Methods
Bordanaba-Florit Guillermo, Felix Royo, Sergei G. Kruglik, Juan M. Falcon-Perez
Summary: Understanding the heterogeneity of extracellular vesicles is crucial for unraveling their functions, but it also poses challenges for their application in medical diagnosis and therapies.
Article
Chemistry, Multidisciplinary
Sergei G. Kruglik, Felix Royo, Jean-Michel Guigner, Laura Palomo, Olivier Seksek, Pierre-Yves Turpin, Irene Tatischeff, Juan M. Falcon-Perez