期刊
JOURNAL OF PHARMACOLOGICAL SCIENCES
卷 116, 期 3, 页码 283-295出版社
JAPANESE PHARMACOLOGICAL SOC
DOI: 10.1254/jphs.10219FP
关键词
asperlin; heme oxygenase (HO)-1; anti-inflammatory effect; RAW264.7 macrophage; murine peritoneal macrophage
资金
- Wonkwang University
Asperlin is a fungal metabolite isolated from Aspergillus sp. SF-5044. In the present study, we isolated asperlin from the marine-derived fungus Aspergillus sp. SF-5044 and demonstrated that it inhibited inducible nitric oxide synthase (iNOS) expression, reduced iNOS-derived NO, suppressed cyclooxygenase (COX)-2 expression, and reduced COX-derived prostaglandin (PG) E-2 production in lipopolysaccharide (LPS)-stimulated RAW264.7 and murine peritoneal macrophages. Similarly, asperlin reduced the production of tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta. In addition, asperlin inhibited the phosphorylation and degradation of I kappa B-alpha, as well as the nuclear translocation of p65 caused by the stimulation of LPS in RAW264.7 macrophages. Furthermore, asperlin induced heme oxygenase (HO)-1 expression through nuclear translocation of nuclear factor E2 related factor 2 and increased HO activity in RAW264.7 macrophages. The effects of asperlin on the LPS-induced expression of iNOS and COX-2 and production of NO, PGE(2), TNF-alpha, and IL-1 beta were partially reversed by a HO-1 inhibitor, tin protoporphyrin. These findings suggest that asperlin-induced HO-1 expression plays a role in the anti-inflammatory effects of asperlin in macrophages.
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