Article
Biochemistry & Molecular Biology
Ewa Tomaszewska, Halyna Rudyk, Izabela Swietlicka, Monika Hulas-Stasiak, Janine Donaldson, Marta Arczewska, Siemowit Muszynski, Piotr Dobrowolski, Maria Mielnik-Blaszczak, Marcin Bartlomiej Arciszewski, Volodymyr Kushnir, Oksana Brezvyn, Viktor Muzyka, Ihor Kotsyumbas
Summary: Exposure to fumonisins during pregnancy negatively impacts offspring bone development by altering bone metabolism and affecting the expression of proteins responsible for bone matrix degradation in a dose- and sex-dependent manner.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Tiina Lehtola, Elina Nummenmaa, Lauri Tuure, Mari Hamalainen, Riina M. Nieminen, Teemu Moilanen, Antti Pemmari, Eeva Moilanen
Summary: Dexamethasone attenuates MMP-13 expression in chondrocytes through MKP-1 and p38 MAP kinase-dependent mechanisms, suggesting MKP-1 as a protective factor and a potential drug target for treating OA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Alison M. Bendele, Madhavi Neelagiri, Venugopal Neelagiri, Irving Sucholeiki
Summary: Osteoarthritis (OA) is a chronic disorder causing damage to cartilage and surrounding tissues, with current treatments providing symptom relief but not affecting disease progression. A new MMP-13 inhibitor, AQU-019, shows potential disease-modifying properties in OA treatment.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biophysics
Tanvir R. Faisal, Malek Adouni, Yasin Y. Dhaher
Summary: This study investigates the relationship between fibril-level degradation and tissue-level response in arthritic diseases using a computational multiscale cartilaginous model. The findings suggest that the relative abundance and spatial characteristics of collagenase and gelatinase play a crucial role in the global and local failure mechanisms of cartilage.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Qichan Hu, Melanie Ecker
Summary: MMP-13 is an attractive target for the treatment of OA due to its involvement in cartilage degradation through cleaving type II collagen. However, there are currently no interventions available to restore degraded cartilage in OA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Yan Zhou, Jianghua Ming, Yaming Li, Bochun Li, Ming Deng, Yonggang Ma, Zhonghui Chen, Yubiao Zhang, Jia Li, Shiqing Liu
Summary: Exosomal miRNA-126-3p derived from SFCs can effectively suppress chondrocyte inflammation and cartilage degeneration, exerting anti-apoptotic and anti-inflammatory effects on articular cartilage, suggesting therapeutic potential for OA treatment.
CELL DEATH DISCOVERY
(2021)
Article
Biochemistry & Molecular Biology
Shahid Parwez, Lalita Panigrahi, Shakil Ahmed, Mohammad Imran Siddiqi
Summary: This study utilizes machine learning-based virtual screening and structure-based rational drug design to identify potential inhibitors of MMP-13 with diverse chemical scaffolds. Two compounds showed potent dose-dependent inhibitory activity and could be further optimized for the design and development of more effective MMP-13 inhibitors.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Medicine, General & Internal
Nuralam Sam, Irawan Yusuf, Irfan Idris, Endy Adnan, Ratna Darjanti Haryadi, Firdaus Hamid, Andry Usman, Muhammad Phetrus Johan, Andi Alfian Zainuddin, Agussalim Bukhari
Summary: This study aimed to investigate the effect of prolotherapy on functional outcome improvements in frozen shoulder patients, as well as the ratio of matrix metalloproteinase-1 (MMP-1)/tissue inhibitor matrix metalloproteinase-1 (TIMP-1) as an indicator of tissue repair. The results showed a significant difference in the ratio of MMP-1/TIMP-1 level between the prolotherapy group and the normal saline group at week 6 and week 12. Both groups performed well in terms of the Numerical Rating Scale score and functional outcome. Prolotherapy changed the mean range of motion in flexion and internal rotation compared to the normal saline group.
Article
Engineering, Biomedical
Allison Mixon, Ahmed Suparno Bahar-Moni, Tanvir R. Faisal
Summary: Matrix metalloproteinases (MMPs) degrade the extracellular matrix (ECM) subsequently damaging cartilage and altering biomechanical properties. Individually, MMP-1 and MMP-9 have minimal effect on cartilage micromechanical properties, but synergistically the two enzymes digest ECM components and result in a greater degradative effect.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Orthopedics
Yunliang Lei, Jiabin Peng, Zhu Dai, Ying Liao, Quanhui Liu, Jian Li, Yonghui Jiang
Summary: The study showed that juvenile articular cartilage fragmentation improves chondrocyte migration by upregulating MT1-MMP. Cell accumulation occurred at the edge of cartilage fragments, leading to better outgrowth compared to cartilage chunks. Overexpression of MT1-MMP enhanced the migration of articular chondrocytes.
Review
Medicine, Research & Experimental
Xingjia Mao, Yanyan Cao, Zijian Guo, Linlin Wang, Chuan Xiang
Summary: Osteoarthritis is a common joint disorder characterized by cartilage degeneration, and circular RNAs have emerged as a popular research topic in influencing various aspects of OA progression. CircRNAs may serve as therapeutic targets and diagnostic biomarkers for OA, suggesting potential roles in effective treatment strategies.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Review
Biology
Yuchen He, Katelyn E. Lipa, Peter G. Alexander, Karen L. Clark, Hang Lin
Summary: Osteoarthritis (OA) is a common degenerative joint disease with no effective treatment currently available. This review examines the similarities between OA chondrocytes and aging cells, and proposes potential methods to reverse the osteoarthritic phenotype. The challenges and future perspectives in OA research are also discussed.
Review
Cell Biology
Anna E. Rapp, Frank Zaucke
Summary: Osteoarthritis (OA) is a common musculoskeletal disease characterized by degradation of cartilage extracellular matrix (ECM). The release of intact/full-length proteins and proteolytic fragments during this degradation process can induce different downstream responses, leading to various biological consequences. Collagen type II and aggrecan are the major components of cartilage ECM, but numerous minor components have also been identified, some of which have been shown to play a role in OA. There is currently no effective therapy for OA, and understanding the matrikine landscape in joint tissues may help identify biomarkers and potential drug targets for disease modification.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Cell Biology
Liangjun Jiang, Kunkun Sheng, Cong Wang, Deting Xue, Zhijun Pan
Summary: Recent studies have shown that MMP2-I1 plays a positive role in the osteogenesis of human bone marrow mesenchymal stem cells and angiogenesis of human vascular endothelial cells. It activates signaling pathways to promote osteogenesis and angiogenesis, and has been found to enhance bone formation and prevent bone loss in animal models.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Huiyu Zhang, Yue Lu, BingBing Wu, Fei Xia
Summary: SEMA3A overexpression improves cell viability and reduces inflammatory injury in LPS-stimulated ATDC5 cells. Overexpression of SEMA3A alleviates LPS-induced apoptosis and ECM degradation in ATDC5 chondrocytes. Interference with NRP-1 disrupts the ameliorative effect of SEMA3A overexpression on LPS-induced chondrocyte inflammation, apoptosis, and ECM degradation.
Article
Biochemistry & Molecular Biology
Bolati Wulaer, Kazuo Kunisawa, Kazuhiro Hada, Willy Jaya Suento, Hisayoshi Kubota, Tsubasa Iida, Aika Kosuge, Taku Nagai, Kiyofumi Yamada, Atsumi Nitta, Yasuko Yamamoto, Kuniaki Saito, Akihiro Mouri, Toshitaka Nabeshima
Summary: Deficiency of Shati/Nat8l affects neurogenesis and attentional behavior in mice, possibly through modulation of dopaminergic transmission.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Oncology
Tetsunari Hase, Masayuki Miyazaki, Kazuya Ichikawa, Naoyuki Yogo, Naoya Ozawa, Takahiro Hatta, Masahiko Ando, Mitsuo Sato, Masashi Kondo, Kiyofumi Yamada, Yoshinori Hasegawa
INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
(2020)
Article
Psychiatry
Mariko Sekiguchi, Akira Sobue, Itaru Kushima, Chenyao Wang, Yuko Arioka, Hidekazu Kato, Akiko Kodama, Hisako Kubo, Norimichi Ito, Masahito Sawahata, Kazuhiro Hada, Ryosuke Ikeda, Mio Shinno, Chikara Mizukoshi, Keita Tsujimura, Akira Yoshimi, Kanako Ishizuka, Yuto Takasaki, Hiroki Kimura, Jingrui Xing, Yanjie Yu, Maeri Yamamoto, Takashi Okada, Emiko Shishido, Toshiya Inada, Masahiro Nakatochi, Tetsuya Takano, Keisuke Kuroda, Mutsuki Amano, Branko Aleksic, Takashi Yamomoto, Tetsushi Sakuma, Tomomi Aida, Kohichi Tanaka, Ryota Hashimoto, Makoto Arai, Masashi Ikeda, Nakao Iwata, Teppei Shimamura, Taku Nagai, Toshitaka Nabeshima, Kozo Kaibuchi, Kiyofumi Yamada, Daisuke Mori, Norio Ozaki
TRANSLATIONAL PSYCHIATRY
(2020)
Article
Neurosciences
Kazuhiro Hada, Bolati Wulaer, Taku Nagai, Norimichi Itoh, Masahito Sawahata, Akira Sobue, Hiroyuki Mizoguchi, Daisuke Mori, Itaru Kushima, Toshitaka Nabeshima, Norio Ozaki, Kiyofumi Yamada
Summary: A study showed that mutations in the Arhgap10 gene associated with schizophrenia may lead to neuronal morphological abnormalities in specific brain regions, which could be related to vulnerability of cognition to methamphetamine treatment.
Article
Biochemistry & Molecular Biology
You-Hsin Lin, Yukie Yamahashi, Keisuke Kuroda, Omar Faruk, Xinjian Zhang, Kiyofumi Yamada, Akihiro Yamanaka, Taku Nagai, Kozo Kaibuchi
Summary: The study revealed that accumbal D2R-MSNs regulate negative reinforcement through the A2aR-PKA-Rap1-MEK pathway. Inhibition of this pathway has been shown to attenuate aversive responses.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Yumi Tsuneura, Masahito Sawahata, Norimichi Itoh, Ryoya Miyajima, Daisuke Mori, Takao Kohno, Mitsuharu Hattori, Akira Sobue, Taku Nagai, Hiroyuki Mizoguchi, Toshitaka Nabeshima, Norio Ozaki, Kiyofumi Yamada
Summary: Reelin, a crucial extracellular matrix protein secreted by Cajal-Retzius cells, plays important roles in brain development and neuronal functions. Reductions in Reelin are associated with brain dysfunctions in mental disorders like schizophrenia. A recent study identified a novel deletion in RELN encoding Reelin in Japanese schizophrenia patients, leading to abnormalities in brain structures and social behavior.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Review
Biochemistry & Molecular Biology
Tsuyoshi Nakai, Kiyofumi Yamada, Hiroyuki Mizoguchi
Summary: Alzheimer's disease is an age-related and progressive neurodegenerative disorder mainly caused by the accumulation of Aβ and tau. Despite various AD mouse models developed, none fully replicate all pathologies present in human AD. APP knock-in mice may offer a better evaluation of candidate therapeutics in early-stage AD mouse models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Akira Mizoguchi, Ryoichi Banno, Runan Sun, Hiroshi Yaginuma, Keigo Taki, Tomoko Kobayashi, Mariko Sugiyama, Taku Tsunekawa, Takeshi Onoue, Hiroshi Takagi, Daisuke Hagiwara, Yoshihiro Ito, Shintaro Iwama, Hidetaka Suga, Taku Nagai, Kiyofumi Yamada, Hiroshi Arima
Summary: This study investigated the role of glucocorticoid receptor signaling in the reward system on the rewarding value of a high-fat diet under restricted caloric stress. Results showed that mice lacking functional GRs in dopaminergic neurons did not exhibit a preference for HFD, in contrast to corticostriatal neuron knockout mice and wild-type mice.
SCIENTIFIC REPORTS
(2021)
Article
Behavioral Sciences
Jingzhu Liao, Geyao Dong, Bolati Wulaer, Masahito Sawahata, Hiroyuki Mizoguchi, Daisuke Mori, Norio Ozaki, Toshitaka Nabeshima, Taku Nagai, Kiyofumi Yamada
Summary: The study found that Reln-del mice exhibited delayed learning in visual discrimination and reversal learning tasks, but their behavioral flexibility remained unaffected.
BEHAVIOURAL BRAIN RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Yumi Tsuneura, Tsuyoshi Nakai, Hiroyuki Mizoguchi, Kiyofumi Yamada
Summary: Reelin, an important protein for brain development and function, is found to be associated with neuropsychiatric disorders. Supplementing Reelin could improve learning and neuronal dysfunctions in animal models. Therefore, studying the role of Reelin in neuropsychiatric disorders and potential therapeutic approaches is of great significance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Md Omar Faruk, Daisuke Tsuboi, Yukie Yamahashi, Yasuhiro Funahashi, You-Hsin Lin, Rijwan Uddin Ahammad, Emran Hossen, Mutsuki Amano, Tomoki Nishioka, Anastasios Tzingounis, Kiyofumi Yamada, Taku Nagai, Kozo Kaibuchi
Summary: The nucleus accumbens (NAc) plays crucial roles in emotional behaviors, and muscarinic signaling induces the phosphorylation of KCNQ2 at T217 via PKC activation for aversive learning. Electric foot shock and donepezil can induce this phosphorylation, while an M1R antagonist can suppress this process.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Tetsuo Matsuzaki, Yoshiaki Kato, Hiroyuki Mizoguchi, Kiyofumi Yamada
Summary: This study developed a predictive model for initial vancomycin dosing using machine learning, and compared its performance with experts, another machine learning model, and commonly used dosing software. The results showed that our model had a comparable target attainment rate to experts, and it could assist in decision making for initial dosing in the absence of dose planning consultations.
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mizuki Uchida, Yukihiro Noda, Sho Hasegawa, Hirotake Hida, Masayuki Taniguchi, Akihiro Mouri, Akira Yoshimi, Toshitaka Nabeshima, Kiyofumi Yamada, Tomomi Aida, Kohichi Tanaka, Norio Ozaki
Summary: The study found that GLAST plays an important role in regulating cognitive and emotional behaviors in mice during neurodevelopment. Dysfunction of early postnatal glutamatergic facilitation by GLAST leads to cognitive and emotional abnormalities, primarily manifested as neurotransmission dysfunction and morphological changes.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Pharmacology & Pharmacy
Masahito Sawahata, Hiroki Asano, Taku Nagai, Norimichi Ito, Takao Kohno, Toshitaka Nabeshima, Mitsuharu Hattori, Kiyofumi Yamada
Summary: The study showed that microinjection of recombinant Reelin into the medial prefrontal cortex prevented recognition memory impairment induced by MK-801, highlighting the potential therapeutic effects of Reelin on cognitive deficits associated with schizophrenia. Despite this positive effect, Reelin treatment did not ameliorate deficits in sensory-motor gating and short-term memory in the animal model of schizophrenia. The results suggest that Reelin acts on its receptors to suppress neural activity in the mPFC, thereby preventing cognitive deficits induced by MK-801.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Cell Biology
Rijwan Uddin Ahammad, Tomoki Nishioka, Junichiro Yoshimoto, Takayuki Kannon, Mutsuki Amano, Yasuhiro Funahashi, Daisuke Tsuboi, Md. Omar Faruk, Yukie Yamahashi, Kiyofumi Yamada, Taku Nagai, Kozo Kaibuchi
Summary: Protein phosphorylation plays a critical role in intracellular signaling pathways and physiological functions in the brain. Dysregulation of these pathways is associated with neurodevelopmental disorders. This study developed an online database to provide information on phosphorylation signals and analyzed related pathways.