Article
Food Science & Technology
Maria Romo, Massimo Castellari, Dinar Fartdinov, Xavier Felipe
Summary: In this study, high-pressure processing was used to remove beta-Lactoglobulin from bovine native whey concentrate and generate an enriched alpha-Lactalbumin fraction. The optimal conditions for alpha-Lactalbumin yield and purification degree were determined. The results showed that high-pressure processing could improve the precipitation of beta-Lactoglobulin and the purification of alpha-Lactalbumin. Further characterization and application of membrane concentration and high-pressure processing are recommended for the valorization of cheese whey.
Article
Food Science & Technology
Maria Romo, Massimo Castellari, Ricard Bou, Pere Gou, Xavier Felipe
Summary: Whey from goat and sheep are being studied for their nutritional properties, but the presence of beta-Lg in these whey sources may cause allergies in infants. Therefore, researchers are interested in developing whey ingredients with higher alpha-La/beta-Lg ratios. This study investigated the effect of high-pressure processing on goat and sheep native whey concentrates (NWC) to obtain alpha-La-enriched fractions. The results showed that high-pressure processing reduced the concentration of beta-Lg in NWC, while alpha-La concentration remained unchanged.
Article
Food Science & Technology
Kungnang Bunsroem, Witoon Prinyawiwatkul, Siwatt Thaiudom
Summary: This study evaluated the susceptibility of whey proteins to enzyme hydrolysis and the antidiabetic properties of protein hydrolysates. The results showed that alpha-LA hydrolysate was more effective than beta-LG hydrolysate in inhibiting DPP-W activity and increasing GLP-1 levels. Heat treatment at 75 degrees C had the greatest impact on the antidiabetic properties of the protein hydrolysates.
Review
Food Science & Technology
Jianxin Xiao, Jiaying Ma, Muhammad Zahoor Khan, Gibson Maswayi Alugongo, Tianyu Chen, Shuai Liu, Shengli Li, Zhijun Cao
Summary: Whey protein components have anticancer attributes and can reduce the risk of colorectal cancer. They have anti-inflammatory and antioxidant abilities and can induce apoptosis, inhibit tumor cell proliferation, and impede metastasis. Studies have shown the importance of incorporating whey protein components into a balanced daily diet for the prevention and treatment of colorectal cancer.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Agriculture, Dairy & Animal Science
Oleksandra Pryshchepa, Gulyaim Sagandykova, Joanna Rudnicka, Pawel Pomastowski, Myroslav Sprynskyy, Boguslaw Buszewski
Summary: One trend in the modern world is the search for biologically active substances based on renewable resources. Milk proteins can be a solution for this, as they have long been known as compounds that can be used to manufacture various food and non-food products. This study aimed to investigate the parameters of Zn-bovine lactoferrin (bLTF) interactions and the synthesis of Zn-rich protein complexes. The results showed that the pH, ionic strength, and concentration of Zn2+ significantly affected the Zn-bLTF interactions.
JOURNAL OF DAIRY SCIENCE
(2022)
Review
Chemistry, Analytical
Jelena Radosavljevic, Dragana Stanic-Vucinic, Marija Stojadinovic, Mirjana Radomirovic, Ana Simovic, Milica Radibratovic, Tanja Cirkovic Velickovic
Summary: Whey protein, an important source of nutrition and biotechnology, is considered a by-product of the dairy industry due to low economic value. The challenge in whey protein separation lies in low concentration and similar properties. Ion exchange techniques are widely used for separation, and recent research on ion exchange membranes shows promise for industrial application. Adsorption-based methodologies targeting protein structure differences are anticipated to further improve whey protein separation.
CURRENT ANALYTICAL CHEMISTRY
(2022)
Article
Agriculture, Dairy & Animal Science
Kamila Puppel, Patrycja Staniszewska, Marcin Golebiewski, Jan Slosarz, Grzegorz Grodkowski, Pawel Solarczyk, Malgorzata Kunowska-Slosarz, Piotr Kostusiak, Beata Kuczynska, Tomasz Przysucha
Summary: This study demonstrates a relationship between whey protein concentrations and blood BHBA levels, serving as markers for diagnosing metabolic diseases. Differences in alpha-lactoalbumin and beta-lactoglobulin concentrations in milk were observed based on varying BHBA levels in cows.
Review
Food Science & Technology
Kevion K. Darmawan, Tom C. Karagiannis, Jeff G. Hughes, Darryl M. Small, Andrew Hung
Summary: This review provides comprehensive information on the structure and function of lactoferrin, as well as its binding with various molecules for food purposes, with a special emphasis on the use of molecular dynamics simulations. The results demonstrate the application of modeling and simulations in determining key residues of lactoferrin responsible for its stability and interactions with other biomolecular components.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Food Science & Technology
Eva Krolitzki, Sebastian P. Schwaminger, Moritz Pagel, Fabian Ostertag, Jorg Hinrichs, Sonja Berensmeier
Summary: Lactoferrin, a versatile protein found in mammals, is primarily isolated from bovine milk or sweet whey for industrial production and consumer goods applications. Established industrial productions focus on skim milk, but alternative approaches utilizing dairy by-products are also able to produce high-quality lactoferrin.
INTERNATIONAL DAIRY JOURNAL
(2022)
Article
Chemistry, Applied
Thomas Valente De Oliveira, Marcelo Depolo Poleto, Samuel Vieira Barbosa, Jane Selia dos Reis Coimbra, Eduardo Basilio De Oliveira
Summary: Computational molecular dynamics was used to analyze the molecular conformations of bovine alpha-lactalbumin at different temperatures, showing that the absence of Ca2+ triggered significant protein conformation changes and affected the foaming properties. This study demonstrates the power of MD in providing insights into the molecular origins of food protein functionalities.
Article
Food Science & Technology
Valentina Gallo, Francesco Giansanti, Alyexandra Arienzo, Giovanni Antonini
Summary: Native and chemically modified whey proteins and their peptide derivatives have various properties, ranging from antimicrobial to immunological and antitumorigenic, which make them potentially useful in a wide range of treatment and prevention strategies. Whey proteins have been found to have antiviral actions against different types of viruses, including SARS-CoV-2. Currently, there are several studies investigating the efficacy of whey proteins, particularly lactoferrin, in human health, specifically in the prevention and treatment of SARS-CoV-2 infection.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Engineering, Chemical
Marlen Gonzalez-Amado, Ana P. M. Tavares, Mara G. Freire, Ana Soto, Oscar Rodriguez
Summary: This study investigated the use of Aqueous Two-Phase Systems (ATPS) for the recovery of lactose, BSA, beta-lactoglobulin and alpha-lactalbumin from cheese whey. Different ATPS were studied for partitioning the selected solutes, showing efficient recovery of proteins and lactose under optimized conditions. This polymer/salt ATPS method could be a scalable and cost-effective separation strategy for cheese whey and related wastes.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Food Science & Technology
Serena Boscaini, Peter Skuse, Kanishka N. Nilaweera, John F. Cryan, Paul D. Cotter
Summary: This review provides an overview of the beneficial effects of whey protein (WP) and its components on human health, particularly in controlling obesity and its comorbidities. WP consumption positively impacts energy balance and obesity-related parameters, primarily through modulation in adipose tissue metabolism and changes that occur in the gastrointestinal tract. Furthermore, WP also has a positive impact on gut microbiota, mood, and behavior. This review emphasizes the importance of WP supplementation in preventing or treating obesity and highlights areas for further research.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Food Science & Technology
Giovanni Barone, Jonathan O'Regan, Alan L. Kelly, James A. O'Mahony
Summary: The study investigated the impact of calcium fortification using different proportions of soluble and insoluble calcium salts on physicochemical and thermal stability properties of a model infant milk formula. Increasing soluble calcium salts decreased the net zeta-potential, increased ionic calcium levels, and raised apparent viscosity, especially at acidic pH. The findings provide useful information for supporting future fortification strategies for nutritional dairy-based products with calcium.
INTERNATIONAL DAIRY JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Kinga Kazimierska, Urszula Kalinowska-Lis
Summary: Milk and colostrum are rich in proteins and possess various properties such as antibacterial, antifungal, antiviral, anticancer, antioxidant, and immunomodulatory. These natural products are used in cosmetics and dermatology, with potential applications in medicine.
Article
Chemistry, Multidisciplinary
Jiang-fan Wan, Gang Wang, Fu-ying Qin, Dan-ling Huang, Yan Wang, Ai-ling Su, Hai-ping Zhang, Yang Liu, Shao-yin Zeng, Chao-liang Wei, Yong-xian Cheng, Jie Liu
Summary: In this study, researchers discovered that a meroterpenoid compound called Z16b isolated from Ganoderma cochlear can effectively inhibit excessive Ca2+ spark frequency and abnormal Ca2+ release events in CPVT models. They found that Z16b interacts with a protein called RyR2, enhancing its stability and preventing CPVT. These findings suggest that Z16b could be a potential novel treatment for CPVT.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Microbiology
Ziwei Hu, Hao Hu, Zhili Hu, Xiaojun Zhong, Yifu Guan, Yunshi Zhao, Lu Wang, Liang Ye, Liliang Ming, Muhammad Shahid Riaz Rajoka, Zhendan He, Yan Wang, Xun Song
Summary: This study investigated the mechanism of sanguinarine (SAN) against Candida albicans. SAN exhibited antifungal activity, induced morphological changes, disrupted cell structure, and damaged cell membranes of C. albicans. In a murine model, SAN significantly improved survival, reduced weight loss, and decreased fungal burden in the kidney. Histopathological examination showed no fungal cells in lung and kidney tissues after SAN treatment. SAN is a promising plant-derived compound for the development of an effective anticandidal agent.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Qian Xie, Yanni Shen, Yanli Meng, Jianhui Liang, Jing Xu, Shishao Liang, Xiaoping Liu, Yan Wang, Chun Hu
Summary: Constitutive activation of the ERK1/2 signaling pathway is commonly observed in various types of tumors. In this study, we report the design and synthesis of novel pyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione derivatives based on compound 1. Among them, compound 14m exhibited excellent antiproliferative activity and demonstrated promising results in inhibiting tumor growth in spheroidal models. Mechanistically, compound 14m suppressed cell migration, induced apoptosis, and modulated the phosphorylation levels of ERK and MEK in a dose-dependent manner.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biology
Ming-Feng He, Jian-Hui Liang, Yan-Ni Shen, Jin-Wen Zhang, Ying Liu, Kuang-Yang Yang, Li-Chu Liu, Junyi Wang, Qian Xie, Chun Hu, Xun Song, Yan Wang
Summary: Glycyrrhizin, an active ingredient in the extract of Glycyrrhizae Radix et Rhizoma, inhibits the entry of SARS-CoV-2 into cells by targeting ACE2.
Article
Plant Sciences
Kuangyang Yang, Qian Xie, Tingting Tang, Na Zhao, Jianhui Liang, Yanni Shen, Ziqi Li, Ben Liu, Jianhai Chen, Wenxiang Cheng, Xueling Bai, Peng Zhang, Qian Liu, Bing Song, Chun Hu, Lichu Liu, Yan Wang
Summary: In this study, the researchers found that Astragaloside IV (ASN IV), a phytochemical found in Astragalus membranaceus, acts as a novel CXCR4 antagonist, protecting cartilage in osteoarthritis (OA) patients. ASN IV inhibits the overexpression of ADAMTS4,5 in chondrocytes by blocking Akt signaling pathway. Furthermore, ASN IV administration repairs damaged cartilage and subchondral bone in an animal model of OA. This study suggests that repurposing ASN IV as a joint protective agent could be a promising strategy for OA drug development.
Article
Biochemistry & Molecular Biology
Qian Liu, Vsevolod Telezhkin, Wenkai Jiang, Yu Gu, Yan Wang, Wei Hong, Weiming Tian, Polina Yarova, Gaofeng Zhang, Simon Ming-yuen Lee, Peng Zhang, Min Zhao, Nicholas D. Allen, Emilio Hirsch, Josef Penninger, Bing Song
Summary: Neural stem cells have the potential for cell replacement therapy, but their tendency to differentiate into glial rather than neuronal phenotypes limits positive outcomes. Physiological electric field (EF) stimulation can boost the neuronal differentiation of neural stem cells without extra chemical side effects, providing a safe and efficient cell therapy for neurological recovery.
CELL AND BIOSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Jinwen Zhang, Mingfeng He, Qian Xie, Ailing Su, Kuangyang Yang, Lichu Liu, Jianhui Liang, Ziqi Li, Xiuxin Huang, Jianshu Hu, Qian Liu, Bing Song, Chun Hu, Lei Chen, Yan Wang
Summary: This study predicts the in vitro and in vivo antiviral activities of drugs by measuring their intracellular bioavailability and biochemical activity. The results show that the antiviral effects of drugs can be predicted without using viruses, which can assist in the early screening of antiviral drugs.
Editorial Material
Pharmacology & Pharmacy
Qian Xie, Kuangyang Yang, Jianhui Liang, Yanni Shen, Lichu Liu, Yan Wang
PHARMACOLOGICAL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Lei Chen, Xia Qiu, Abigail Dupre, Oscar Pellon-Cardenas, Xiaojiao Fan, Xiaoting Xu, Prateeksha Rout, Katherine D. Walton, Joseph Burclaff, Ruolan Zhang, Wenxin Fang, Rachel Ofer, Alexandra Logerfo, Kiranmayi Vemuri, Sheila Bandyopadhyay, Jianming Wang, Gaetan Barbet, Yan Wang, Nan Gao, Ansu O. Perekatt, Wenwei Hu, Scott T. Magness, Jason R. Spence, Michael P. Verzi
Summary: This study identified the role of TGFB signaling in intestinal regeneration following injury. TGFB1 was found to induce fetal-like transcriptional signature and enhance the regenerative response in the epithelium. Additionally, TGFB1 treatment enhanced the ability of primary epithelial cultures to engraft into damaged murine colon, suggesting potential for cellular therapy.
Article
Plant Sciences
Kuangyang Yang, Qian Xie, Jiaxin Liao, Na Zhao, Jianhui Liang, Ben Liu, Jianhai Chen, Wenxiang Cheng, Xueling Bai, Peng Zhang, Qian Liu, Bing Song, Junyi (Danny) Wang, Fanghao Zheng, Chun Hu, Lichu Liu, Lei Chen, Yan Wang
Summary: The traditional Chinese medicine formula SKHS and its main phytochemical coptisine were found to protect cartilage and inhibit CXCR4-mediated ADAMTS4,5 overexpression, suggesting their potential for OA treatment. The study also identified coptisine as a potent CXCR4 antagonist. This research provides important insights into the molecular mechanisms of SKHS and coptisine for OA treatment.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ming-Feng He, Jian-Hui Liang, Yan-Ni Shen, Chao-Wei Zhang, Kuang-Yang Yang, Li-Chu Liu, Qian Xie, Chun Hu, Xun Song, Yan Wang
Summary: This study screened Chinese herbs for their ability to activate human immunity and identified coptisine as a potent inhibitor of the influenza virus. Pre-treatment with coptisine was more effective at reducing viral replication than co-treatment or post-treatment. Coptisine upregulated the p21 signaling pathway in MDCK cells and inhibited MELK activity directly.
Editorial Material
Pharmacology & Pharmacy
Wenxin Fang, Ruolan Zhang, Jianhui Liang, Wanrong Xie, Youtao Zhang, Lei Chen, Yan Wang
PHARMACOLOGICAL RESEARCH
(2023)
Article
Plant Sciences
Kuangyang Yang, Qian Xie, Jianhui Liang, Yanni Shen, Ziqi Li, Na Zhao, Yuanyan Wu, Lichu Liu, Peng Zhang, Chun Hu, Lei Chen, Yan Wang
Summary: By analyzing data and conducting experiments, we found that the Chinese medicinal herb Andrographis paniculata inhibits the activity of FABP4, which contributes to cartilage degradation in osteoarthritis (OA) patients.
Article
Pharmacology & Pharmacy
Sameera Senaweera, Tiffany C. Edwards, Jayakanth Kankanala, Yan Wang, Rajkumar Lalji Sahani, Jiashu Xie, Robert J. Geraghty, Zhengqiang Wang
Summary: This study discovered a novel antiviral drug for human cytomegalovirus (HCMV) infections, which showed promising antiviral activity and drug-like properties. The structure-activity relationship analysis identified key pharmacophore features and several potent analogs. Preliminary studies on the mechanism of action and ADME properties demonstrated the potential of this drug as an effective treatment for HCMV infections.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Chemistry, Multidisciplinary
Qian Xie, Yanni Shen, Jianhui Liang, Chao Zhang, Xianwu Ling, Liangxiao Gu, Yiling Wang, Yan Wang, Xiaoping Liu, Chun Hu
Summary: In this study, a series of compounds were synthesized and compound XS23 showed excellent anti-tumor activity. XS23 was found to inhibit cell migration and proliferation in A375 cells and induce cell apoptosis by elevating ROS production.
NEW JOURNAL OF CHEMISTRY
(2022)
