Article
Multidisciplinary Sciences
Mia Krout, Kelly H. Oh, Ame Xiong, Elisa B. Frankel, Peri T. Kurshan, Hongkyun Kim, Janet E. Richmond
Summary: Synaptic transmission requires the coordinated activity of multiple synaptic proteins at the active zone (AZ). CLA-1 and UNC-10 work together to regulate the levels of the UNC-2 calcium channel and the localization of UNC-13 at the synapse. These findings suggest a conserved arrangement of AZ scaffolding proteins that are crucial for precise coupling to Ca2+ channels.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Scott P. Ginebaugh, Yomna Badawi, Tyler B. Tarr, Stephen D. Meriney
Summary: The mouse neuromuscular junction (NMJ) has been widely used as a model synapse to study neurotransmission. Understanding the relationships within the highly organized active zones (AZs) is crucial for healthy synapses and the pathophysiology of neuromuscular diseases.
Review
Biochemistry & Molecular Biology
Pragya Goel, Dion Dickman
Summary: Homeostatic signaling systems play a crucial role in maintaining stable functionality in changing environments by regulating the balance of excitation and inhibition at synapses. Research on the Drosophila neuromuscular junction has identified five homeostats that work independently yet simultaneously to balance neurotransmission. These ancient signaling systems are believed to have emerged early in evolution and play a fundamental role in stabilizing mammalian cholinergic NMJs and glutamatergic central synapses.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Neurosciences
Kosala N. Dissanayake, Filip Margetiny, Charlotte L. Whitmore, Robert C-C Chou, Cornelia Roesl, Vishwendra Patel, Joseph J. McArdle, Richard Webster, David Beeson, John E. H. Tattersall, David J. A. Wyllie, Michael Eddleston, Richard R. Ribchester
Summary: Intentional ingestion of agricultural organophosphorus insecticides is a significant public health issue in rural Asia, leading to thousands of deaths annually. Cyclohexanol alters synaptic transmission at neuromuscular junctions, reducing postsynaptic sensitivity to acetylcholine while enhancing evoked transmitter release.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Cell Biology
Chulpan R. Gafurova, Andrei N. Tsentsevitsky, Alexey M. Petrov
Summary: The study revealed that different types of synaptic vesicles (SVs) in nerve terminals exhibit distinct behaviors in response to low- and high-frequency stimulations. At least two SV pools were found to operate during prolonged stimulation, one for maintaining release at different frequencies and the other for responding to increasing activity.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ellya Bukharaeva, Venera Khuzakhmetova, Svetlana Dmitrieva, Andrei Tsentsevitsky
Summary: Adrenoceptor activators and blockers are commonly used in the clinical treatment of cardiovascular, pulmonary, and neurodegenerative diseases by regulating neurotransmission at the neuromuscular junction. Understanding the molecular mechanisms of these drugs may lead to the development of highly effective and well-tolerated sympathomimetics for treating various neurodegenerative diseases with synaptic defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Ryan J. Durbin, Dante J. Heredia, Thomas W. Gould, Robert B. Renden
Summary: Neurotransmission is influenced by extracellular pH. Alkalization enhances transmitter release and receptor activation, while acidification inhibits these processes and can activate acid-sensitive conductances in the synaptic cleft. The direction of change differs across synapse types. At the mammalian neuromuscular junction (NMJ), the direction and magnitude of pH transients in the synaptic cleft during transmission remain unclear. In this study, researchers investigated the extracellular pH transients at the cholinergic synapse of the mouse NMJ under near physiological conditions and identified their sources. They found that activity-induced intracellular Ca2+ release in the muscle plays a role in cleft alkalization, whereas PMCA inhibition decreases alkalization. This suggests that cholinergic synapses at the mouse NMJ typically alkalize due to cytosolic Ca2+ liberation during activity, unless under highly strenuous conditions where acidification predominates.
JOURNAL OF NEUROSCIENCE
(2023)
Review
Pharmacology & Pharmacy
Onsurang Wattanathamsan, Varisa Pongrakhananon
Summary: This review article focuses on the role of microtubule-associated proteins (MAPs) in cancer aggressiveness, particularly cancer metastasis activity. It highlights the importance of altered MAP expression in various cancer types and its association with cancer progression. The article also discusses the relevance of MAPs and related molecular signaling pathways in cancer metastasis. It provides comprehensive understanding of MAP function on microtubules and its implications in cancer management as potential therapeutic targets and prognostic markers.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Medicine, Research & Experimental
Alexey M. Petrov, Guzalia F. Zakirjanova, Irina Kovyazina, Andrei N. Tsentsevitsky, Ellya A. Bukharaeva
Summary: This study investigated the influence of nerve activity and adrenoceptor stimulation on exocytosis modes in the motor nerve terminal. It was found that high frequency activity promoted neurotransmitter release via kiss-and-run mechanism, while noradrenaline and alpha 2-adrenoreceptors agonist favored full-collapse exocytosis. This has implications for the regulation of respiratory system during stress.
Article
Neurosciences
Kaikai He, Yifu Han, Xiling Li, Roberto X. Hernandez, Danielle Riboul, Touhid Feghhi, Karlis A. Justs, Olena Mahneva, Sarah Perry, Gregory T. Macleod, Dion Dickman
Summary: Neurons exhibit functional diversity and can be classified into tonic or phasic patterns of activity. The synaptic differences between tonic and phasic neurons have remained unclear, but this study found major distinctions in neurotransmitter release properties, Ca2+ influx, and synaptic vesicle coupling between these two types of neurons. The organization and stoichiometry of voltage-gated Ca2+ channels at phasic neuron release sites were also observed to be different. These findings suggest that active zone nano-architecture and Ca2+ influx contribute to differential glutamate release at tonic and phasic synapses.
JOURNAL OF NEUROSCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Kenji Takikawa, Hiroshi Nishimune
Summary: Synaptic transmission is crucial for motor control and brain functions. Presynaptic molecules play a vital role in regulating neurotransmitter release. Dysfunction of presynaptic molecules can lead to various neurological disorders and cognitive decline in aging.
Article
Multidisciplinary Sciences
Zhe Chen, Ming Li, Hao Zhu, Guangshuo Ou
Summary: Cilia have distinct subdomains with variable axonemal structures that perform diverse functions in cell motility and signaling. In sensory cilia, the axoneme differentiates into a middle segment with nine microtubule doublets and a distal segment with nine microtubule singlets extending from the A tubules of the doublets. This study investigates axoneme differentiation in Caenorhabditis elegans and identifies the role of the flagellar inner junction protein FAP20 and PCRG1 in connecting A and B tubules. Loss of CFAP-20 disconnects the A and B tubules, while the absence of PCRG-1 does not disrupt cilia. Ectopic introduction of PCRG-1 into cilia leads to abnormal MT doublets in the distal segment and impairs intraflagellar transport and animal sensation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Neurosciences
Yupu Wang, Meike Lobb-Rabe, James Ashley, Veera Anand, Robert A. Carrillo
Summary: In Drosophila larval neuromuscular junctions, ablating specific types of motor neurons can lead to structural changes and increased neurotransmitter release at other motor neuron synapses, demonstrating synaptic plasticity. Some synapses exhibit compensatory plasticity, while silencing one type of motor neuron can affect the plasticity of other types of synapses.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biology
Alireza Ghasemizadeh, Emilie Christin, Alexandre Guiraud, Nathalie Couturier, Marie Abitbol, Valerie Risson, Emmanuelle Girard, Christophe Jagla, Cedric Soler, Lilia Laddada, Colline Sanchez, Francisco-Ignacio Jaque-Fernandez, Vincent Jacquemond, Jean-Luc Thomas, Marine Lanfranchi, Julien Courchet, Julien Gondin, Laurent Schaeffer, Vincent Gache
Summary: This study demonstrates the essential role of microtubule-associated protein MACF1 in regulating myonuclei positioning and maintenance in skeletal muscles. MACF1 deficiency leads to abnormal myonuclei positioning and microtubule network organization, as well as reduced muscle excitability while maximal muscle force remains unchanged. Additionally, MACF1-KO mice exhibit improved resistance to fatigue, associated with a significant increase in mitochondria biogenesis.
Article
Neurosciences
Joseph A. Szule
Summary: This report proposes a unified hypothesis for the regulation of vesicle trafficking events in neuronal synapses by integrating knowledge of macromolecular structures and synaptic protein biochemistry. The hypothesis combines electron tomography with biochemical studies to characterize the different classes of macromolecules involved in these events. The goal is to guide future research in understanding how the Active Zone Material regulates neurotransmitter secretion.
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2022)
Article
Cell Biology
Anne-Laure Bouge, Marie-Laure Parmentier
DISEASE MODELS & MECHANISMS
(2016)
Article
Multidisciplinary Sciences
Elodie Urlacher, Laurent Soustelle, Marie-Laure Parmentier, Heleen Verlinden, Marie-Julie Gherardi, Daniel Fourmy, Alison R. Mercer, Jean-Marc Devaud, Isabelle Massou
Article
Multidisciplinary Sciences
Margarita Arango-Lievano, Camille Peguet, Matthias Catteau, Marie-Laure Parmentier, Synphen Wu, Moses V. Chao, Stephen D. Ginsberg, Freddy Jeanneteau
SCIENTIFIC REPORTS
(2016)
Article
Neurosciences
Marine Pons, Claire Soulard, Laurent Soustelle, Marie-Laure Parmentier, Yves Grau, Sophie Layalle
FRONTIERS IN CELLULAR NEUROSCIENCE
(2017)
Article
Multidisciplinary Sciences
Isabelle Devambez, Juliette van Dijk, Salim Benlefki, Sophie Layalle, Yves Grau, Krzysztof Rogowski, Marie-Laure Parmentier, Laurent Soustelle
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Yasmina Talmat-Amar, Yoan Arribat, Marie-Laure Parmentier
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Biochemistry & Molecular Biology
Veronique Morel, Simon Lepicard, Alexandre N. Rey, Marie-Laure Parmentier, Laurent Schaeffer
CELLULAR AND MOLECULAR LIFE SCIENCES
(2014)
Article
Neurosciences
Christelle Redt-Clouet, Severine Trannoy, Ana Boulanger, Elena Tokmatcheva, Elena Savvateeva-Popova, Marie-Laure Parmentier, Thomas Preat, Jean-Maurice Dura
EUROPEAN JOURNAL OF NEUROSCIENCE
(2012)
Article
Biochemistry & Molecular Biology
Yasmina Talmat-Amar, Yoan Arribat, Christelle Redt-Clouet, Sebastien Feuillette, Anne-Laure Bouge, Magalie Lecourtois, Marie-Laure Parmentier
HUMAN MOLECULAR GENETICS
(2011)
Meeting Abstract
Clinical Neurology
F. Maschat, Y. Arribat, Y. Talmat-Amar, N. Bonneaud, C. Bauer, J. Bayle, P. Maurel, M-L Parmentier
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2012)
Article
Multidisciplinary Sciences
Isabelle Devambez, Moutaz Ali Agha, Christian Mitri, Joel Bockaert, Marie-Laure Parmentier, Frederic Marion-Poll, Yves Grau, Laurent Soustelle
Article
Multidisciplinary Sciences
Yoan Arribat, Nathalie Bonneaud, Yasmina Talmat-Amar, Sophie Layalle, Marie-Laure Parmentier, Florence Maschat
Article
Neurosciences
Yoan Arribat, Yasmina Talmat-Amar, Alexia Paucard, Pierre Lesport, Nathalie Bonneaud, Caroline Bauer, Nicole Bec, Marie-Laure Parmentier, Lorraine Benigno, Christian Larroque, Patrick Maurel, Florence Maschat
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2014)
Article
Cell Biology
Aurore Filaquier, Philippe Marin, Marie-Laure Parmentier, Julien Villeneuve
Summary: There are multiple routes for protein secretion in eukaryotes, known as unconventional protein secretion (UPS), in addition to the conventional endoplasmic reticulum-Golgi secretory pathway. These UPS mechanisms are diverse but share common principles, and they play a significant role in adaptive responses to specific cellular needs and stresses.
CURRENT OPINION IN CELL BIOLOGY
(2022)