Article
Neurosciences
Prudhvi Raj Rayi, Hanoch Kaphzan
Summary: Angelman syndrome is a neurogenetic disorder characterized by developmental delay, speech impairment, and other symptoms. Studies have shown that hippocampal deficits in AS mice may be related to altered calcium dynamics and increased alpha 1-Na/K-ATPase expression levels. However, the causal link between hippocampal deficits and major behavioral phenotypes in AS is still unclear.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Neurosciences
Jie Li, Mark L. Baccei
Summary: In a subset of glutamatergic interneurons in the neonatal spinal superficial dorsal horn (SDH), there is intrinsic burst-firing activity regulated by persistent Na+ channels and inward-rectifying K+ channels, which decreases during postnatal development. While ascending lamina I projection neurons targeting the parabrachial nucleus (PB) or periaqueductal gray (PAG) can also display pacemaker activity in early life, the mechanisms driving this activity and its conservation across cell types in the spinal dorsal horn remain unclear. Our study demonstrates that adolescent projection neurons retain the ability to generate pacemaker activity, with differences in membrane properties compared to adjacent neurons lacking intrinsic burst-firing. Further research is needed to understand the mechanisms governing pacemaker activity in the major output neurons of the SDH network.
NEUROSCIENCE LETTERS
(2021)
Article
Neurosciences
Nicolas Fourcaud-Trocme, Mickael Zbili, Patricia Duchamp-Viret, Nicola Kuczewski
Summary: In the olfactory bulb, mitral cells (MCs) exhibit spontaneous bursting activity, characterized by bursts of action potentials (APs) intermixed with silent periods. The burst initiation threshold and duration are dependent on the membrane potential, which in turn is regulated by the inactivation state of voltage-dependent Na+ channels. The fast afterhyperpolarization (AHP) promotes burst firing, while the slow AHP contributes to burst termination.
Article
Multidisciplinary Sciences
Zeinab Jahed, Yang Yang, Ching-Ting Tsai, Ethan P. Foster, Allister F. McGuire, Huaxiao Yang, Aofei Liu, Csaba Forro, Zen Yan, Xin Jiang, Ming-Tao Zhao, Wei Zhang, Xiao Li, Thomas Li, Annalisa Pawlosky, Joseph C. Wu, Bianxiao Cui
Summary: This study develops mechanically robust nanocrown electrodes with high success rates achieved through electroporation, and validates their accurate recording of intracellular action potentials through simultaneous patch clamp recording. The study also demonstrates the ability of nanocrown electrodes to enable prolonged recording of the same cells after sequential addition of drugs. This technological advancement is significant for establishing a screening assay for preclinical evaluation of drug-induced arrhythmogenicity.
NATURE COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Fiona O'Brien, Claire H. Feetham, Caroline A. Staunton, Kathryn Hext, Richard Barrett-Jolley
Summary: The transient receptor potential cation channel subfamily V Member 4 (Trpv4) in the paraventricular nucleus (PVN) of the hypothalamus plays an important role in temperature sensitivity of PVN neurons, affecting their activity frequency. At physiological temperatures, the activity frequency of PVN neurons is primarily regulated by Trpm2 channels, while at room temperature, it is mainly regulated by reduced Trpv4 activity.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Sion Park, Pia Fluethmann, Carla Wolany, Lena Goedecke, Hannah Maleen Spenner, Thomas Budde, Hans-Christian Pape, Kay Juengling
Summary: The neuropeptide S system, consisting of the NPS and NPSR1, has been extensively studied in rodents. Activation of NPSR1 in amygdalar neurons inhibits voltage-gated potassium channels and increases neuronal excitability through G(alpha q) signaling and calcium ions.
Article
Biology
Crescent L. Combe, Carol M. Upchurch, Carmen C. Canavier, Sonia Gasparini
Summary: A combination of in vitro electrophysiology and modeling identified TRPM4 channels as major drivers of cholinergic modulation of firing rate in rat CA1 pyramidal neurons. Cholinergic agonist carbachol reverses the spike rate adaptation caused by Na-V channel inactivation, resulting in more spikes on the down-ramp compared to the up-ramp. Pharmacological experiments and modeling suggest that TRPM4 channels play a role in the cholinergic shift of firing center of mass through Ca2+-activated nonspecific cation current ICAN.
Article
Biochemistry & Molecular Biology
Anna M. Olszewska, Adam K. Sieradzan, Piotr Bednarczyk, Adam Szewczyk, Michal A. Zmijewski
Summary: The study reveals that calcitriol can directly affect the activity of the mitochondrial potassium channel in human astrocytoma cells, suggesting its potential role in regulating mitochondrial function.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2022)
Article
Pharmacology & Pharmacy
Young-Woo Nam, Meng Cui, Naglaa Salem El-Sayed, Razan Orfali, Misa Nguyen, Grace Yang, Mohammad Asikur Rahman, Judy Lee, Miao Zhang
Summary: The selectivity of CyPPA among K(Ca)2 and K(Ca)3.1 channel subtypes relies on the HA/HB helices.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Neurosciences
Kun Zhang, Yanfei Han, Peng Zhang, Yuqiong Zheng, Aobing Cheng
Summary: The study developed a multiplexed method to measure neurotransmitter dynamics using simultaneous whole-cell patch clamp recordings and genetically encoded fluorescence sensor imaging. Results showed that the genetically encoded sensors and electrophysiological recordings did not interfere with each other, and each technique had its own strengths and weaknesses in detecting different neurotransmitters. This study highlights the potential of combined techniques for future multianalyte monitoring.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Geriatrics & Gerontology
Ingrid Nogueira, Thiago Z. Lima, Thawann Malfatti, Katarina E. Leao
Summary: This study compares the effects of noise exposure on membrane properties of neurons in the auditory cortex of mice. The results show that different types of neurons exhibit distinct changes after noise exposure, which may affect the activity levels of the auditory system.
FRONTIERS IN AGING NEUROSCIENCE
(2023)
Article
Engineering, Biomedical
William G. A. Brown, Karina Needham, James M. Begeng, Alexander C. Thompson, Bryony A. Nayagam, Tatiana Kameneva, Paul R. Stoddart
Summary: Infrared light has the potential to modulate neuronal activity through thermally-evoked currents in thermosensitive ion channels. Patch-clamp recordings from cultured SGNs showed that the stimulation threshold for SGNs may not be as low as previously reported in vivo, calling into question the potential use of this effect for auditory prostheses. A Hodgkin-Huxley-type model accurately simulated neuronal responses and predicted action potential thresholds in stimulation parameter spaces not accessible during the study.
JOURNAL OF NEURAL ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Shuyi Tan, Xiuping Mo, Huihui Qin, Binbin Dong, Jiankui Zhou, Cheng Long, Li Yang
Summary: This article presents a detailed methodology for revealing the electrophysiological and morphological characteristics of neurons, using single-cell labeling combined with whole-cell patch-clamp recording. The study demonstrates the properties of different types of neurons in brain slices and also uncovers defects in specific neurons in knockout mice.
Article
Biochemical Research Methods
Tori Lhomme, Vincent Prevot
Summary: This article presents a protocol for tanycyte-neuron paired whole-cell patch-clamp recording in living mouse brain slices. It provides detailed steps for mice generation, solution preparation, and dissection, as well as the realization of slices and patch-clamp recordings. The protocol can be adapted to study metabolic coupling between tanycytes and any neuronal population.
Article
Biochemistry & Molecular Biology
Tengiz Oniani, Laura Vinnenberg, Rahul Chaudhary, Julian A. Schreiber, Kathrin Riske, Brandon Williams, Hans-Christian Pape, John A. White, Anna Junker, Guiscard Seebohm, Sven G. Meuth, Petra Hundehege, Thomas Budde, Mehrnoush Zobeiri
Summary: This study investigates the effects of MS-related factors on HCN channels and finds that HCN channels are not directly influenced by trace metals and divalent cation chelators.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Developmental Biology
Peter Kirwan, Benita Turner-Bridger, Manuel Peter, Ayiba Momoh, Devika Arambepola, Hugh P. C. Robinson, Frederick J. Livesey
Article
Neurosciences
Hugo Zeberg, Hugh P. C. Robinson, Peter Arhem
JOURNAL OF NEUROPHYSIOLOGY
(2015)
Article
Neurosciences
James L. Butler, Philipe R. F. Mendonca, Hugh P. C. Robinson, Ole Paulsen
JOURNAL OF NEUROSCIENCE
(2016)
Article
Biology
Philipe R. F. Mendonca, Mariana Vargas-Caballero, Ferenc Erdelyi, Gabor Szabo, Ole Paulsen, Hugh P. C. Robinson
Article
Biochemistry & Molecular Biology
Hugh P. C. Robinson, Leanne Li
Article
Oncology
Leanne Li, Qiqun Zeng, Arjun Bhutkar, Jose A. Galvan, Eva Karamitopoulou, Daan Noordermeer, Mei-Wen Peng, Alessandra Piersigilli, Aurel Perren, Inti Zlobec, Hugh Robinson, M. Luisa Iruela-Arispe, Douglas Hanahan
Article
Neurosciences
Philipe R. F. Mendonca, Victoria Kyle, Shel-Hwa Yeo, William H. Colledge, Hugh P. C. Robinson
JOURNAL OF PHYSIOLOGY-LONDON
(2018)
Article
Neurosciences
Xiumin Li, Kenji Morita, Hugh P. C. Robinson, Michael Small
JOURNAL OF NEUROPHYSIOLOGY
(2013)
Article
Neurosciences
William A. Catterall, Indira M. Raman, Hugh P. C. Robinson, Terrence J. Sejnowski, Ole Paulsen
JOURNAL OF NEUROSCIENCE
(2012)
Article
Mathematical & Computational Biology
Cristian Carmeli, Paolo Bonifazi, Hugh P. C. Robinson, Michael Small
FRONTIERS IN COMPUTATIONAL NEUROSCIENCE
(2013)
Article
Mathematical & Computational Biology
Michael Vella, Robert C. Cannon, Sharon Crook, Andrew P. Davison, Gautham Ganapathy, Hugh P. C. Robinson, R. Angus Silver, Padraig Gleeson
FRONTIERS IN NEUROINFORMATICS
(2014)
Article
Multidisciplinary Sciences
Qiqun Zeng, Iacovos P. Michael, Peng Zhang, Sadegh Saghafinia, Graham Knott, Wei Jiao, Brian D. McCabe, Jose A. Galvan, Hugh P. C. Robinson, Inti Zlobec, Giovanni Ciriello, Douglas Hanahan
Article
Biochemistry & Molecular Biology
Hugo Zeberg, Michael Dannemann, Kristoffer Sahlholm, Kristin Tsuo, Tomislav Maricic, Victor Wiebe, Wulf Hevers, Hugh P. C. Robinson, Janet Kelso, Svante Paeaebo
Article
Biophysics
Christian Scheppach, Hugh P. C. Robinson
BIOPHYSICAL JOURNAL
(2017)
Meeting Abstract
Clinical Neurology
H. Zeberg, H. P. C. Robinson, P. Arhem