Article
Materials Science, Biomaterials
Dilka Liyana Arachchige, Sushil K. Dwivedi, May Waters, Sophia Jaeger, Joe Peters, Daniel R. Tucker, Micaela Geborkoff, Thomas Werner, Rudy L. Luck, Bhaskar Godugu, Haiying Liu
Summary: This study developed a series of near-infrared fluorescent probes for sensing NAD(P)H. Probes A and B exhibited excellent fluorescence performance in the near-infrared range, with minimal interference and photodamage. These probes are useful for monitoring changes in NAD(P)H levels during metabolic processes. Additionally, probe A has been used to assess the impact of chemotherapy drugs on cancer cells and to detect changes in NAD(P)H levels under hypoxic conditions.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Cell Biology
Elena V. Mitroshina, Maria O. Savyuk, Evgeni Ponimaskin, Maria V. Vedunova
Summary: Hypoxia is a common pathological condition induced by various events, with the body's adaptation to it being crucial for health and disease. HIFs, a family of transcription factors, play a key role in cellular responses to hypoxia and are increasingly viewed as potential targets for treating a range of hypoxia-associated diseases. The role of HIFs in adaptation to hypoxia is universal across tissue types, including the CNS, where they are involved in regulating neurogenesis, nerve cell differentiation, and neuronal apoptosis, potentially offering new therapeutic opportunities.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Carolyne Lespay-Rebolledo, Andrea Tapia-Bustos, Ronald Perez-Lobos, Valentina Vio, Emmanuel Casanova-Ortiz, Nancy Farfan-Troncoso, Marta Zamorano-Cataldo, Martina Redel-Villarroel, Fernando Ezquer, Maria Elena Quintanilla, Yedy Israel, Paola Morales, Mario Herrera-Marschitz
Summary: Labor and delivery involve a complex cascade of metabolic and physiological reactions, which usually result in successful childbirth. However, if the oxygen supply is interrupted, it can lead to perinatal asphyxia, causing energy failure and cell dysfunction. Perinatal asphyxia has long-term effects on the brain's ability to handle stressors. Studies have identified targets responsible for metabolic cascades linked to neurodevelopmental impairments caused by perinatal asphyxia in rats. The brain damage continues even after re-oxygenation, affecting neurons and glial cells.
Article
Biochemistry & Molecular Biology
Micol Silic-Benussi, Evgeniya Sharova, Alberto Corradin, Loredana Urso, Vittoria Raimondi, Ilaria Cavallari, Barbara Buldini, Samuela Francescato, Sonia A. Minuzzo, Donna M. D'Agostino, Vincenzo Ciminale
Summary: The combination of everolimus and verapamil has been found to enhance the anti-oxidative and cell death effects on T-ALL cells. This combination therapy also showed significant inhibition of tumor growth in refractory T-ALL, providing a potential treatment option for patients who do not respond to standard chemotherapy.
Article
Chemistry, Multidisciplinary
Yuxiang Wang, Jing Chen, Rumeng Duan, Rong Gu, Weiran Wang, Jinhui Wu, Huibo Lian, Yiqiao Hu, Ahu Yuan
Summary: In situ tumor vaccination is achieved through the integration of Phy@PLGdH nanosheets, which enhances radiation therapy-induced immunogenic cell death. The nanosheets exhibit improved radiosensitization and tumor penetrability, while Phy-mediated PPP intervention amplifies the oxidative stress and DNA damage caused by PLGdH-sensitized radiation therapy. This results in effective immunogenic cell death and enhanced immunogenicity of irradiated tumor cells. Furthermore, Phy@PLGdH-sensitized radiation therapy successfully triggers a robust CD8(+)-T-cell-dependent antitumor immune response, which enhances checkpoint blockade immunotherapies against primary and metastatic tumors.
ADVANCED MATERIALS
(2022)
Article
Oncology
Fan Zhang, Zhiwei Wu, Bowen Yu, Zhengping Ning, Zhixing Lu, Liang Li, Fei Long, Qionggui Hu, Chonglei Zhong, Yi Zhang, Changwei Lin
Summary: In this study, ATP13A2 was identified as a novel gene associated with the PPP pathway. ATP13A2 deficiency inhibited CRC growth and PPP activity, while ATP13A2 overexpression had the opposite effect. Mechanistically, ATP13A2 regulated the PPP by affecting PGD mRNA expression. Furthermore, ATP13A2 knockdown inhibited CRC growth in PDO and PDX models. The study revealed that ATP13A2 overexpression-driven dephosphorylation of TFEB promotes PPP activation by increasing PGD transcription, suggesting ATP13A2 as a potential target for CRC therapy.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Zhaomin Mao, Ying Tan, Juan Tao, Linlin Li, Feng Yu, Minghui Zhao
Summary: Recent studies suggest a link between metabolic disorders and the development of SLE and LN. This study used proteomics to identify metabolic reprogramming in the kidneys of LN patients. The results showed an enrichment of the pentose phosphate pathway (PPP) in LN kidneys. High expression of the key enzyme, glucose-6-phosphate dehydrogenase (G6PD), was associated with worse prognosis in LN patients. Further analysis revealed involvement of the mTORC1 signaling pathway in G6PD-associated tubular damage induced by IgG-LN.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Review
Pharmacology & Pharmacy
Baoqi Yu, Xia Wang, Yanting Song, Guomin Xie, Shiyu Jiao, Li Shi, Xuejie Cao, Xinyao Han, Aijuan Qu
Summary: Cardiovascular diseases are the leading cause of death worldwide, and hypoxia-inducible factors (HIFs) play an important role in their pathogenesis. Recent studies have revealed the role of cell-specific HIFs in various cardiovascular diseases, but the potential clinical application of HIF inhibitors in the treatment of cardiovascular diseases is not well understood.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Multidisciplinary Sciences
Claudia Montllor-Albalate, Hyojung Kim, Anna E. Thompson, Alex P. Jonke, Matthew P. Torres, Amit R. Reddi
Summary: Cu/Zn superoxide dismutase (Sod1) acts as an important antioxidant enzyme, promoting NADPH production and balancing glycolytic and oxPPP flux by controlling GAPDH activity to adapt to life in air. Sod1-derived H2O2 is crucial for antioxidant defense and serves as a master regulator of metabolism and the thiol redoxome, impacting numerous metabolic enzymes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Agriculture, Dairy & Animal Science
Lin Zhu, Chunling Bai, Xueqiao Wang, Zhuying Wei, Mingjuan Gu, Xinyu Zhou, Guanghua Su, Xuefei Liu, Lei Yang, Guangpeng Li
Summary: The knockdown of MSTN accelerates the pentose phosphate pathway, thereby enhancing the antioxidant capacity of erythrocytes. These findings provide important insights into the role of MSTN in erythrocyte antioxidant regulation after exhaustive exercise.
Article
Biochemistry & Molecular Biology
Pamela De Falco, Giacomo Lazzarino, Federica Felice, Enrico Desideri, Serena Castelli, Illari Salvatori, Fabio Ciccarone, Maria Rosa Ciriolo
Summary: N-acetylaspartate (NAA) is synthesized by the mitochondrial enzyme NAT8L using acetyl-CoA and aspartate as substrates. The modulation of NAT8L expression has been found to affect the metabolic adaptations and proliferative capability of hepatocellular carcinoma cells. NAT8L downregulation increases cell proliferation, while over-expression decreases cell growth. Mechanistically, NAT8L downregulation promotes aspartate outflow from mitochondria to enhance glucose flux into the pentose phosphate pathway, resulting in increased purine biosynthesis. These findings suggest that NAT8L plays a critical role in regulating cell proliferation through metabolic rewiring.
Article
Biochemistry & Molecular Biology
Jin-Woo Kim, Hyun-Ju An, HyunJeong Yeo, Yunhui Jeong, HyeonHae Lee, Jusung Lee, Kisik Nam, Jongheon Lee, Dong-Eun Shin, Soonchul Lee
Summary: Activation of the HIF-1α signaling pathway can protect intervertebral discs from degeneration and may serve as a potential therapeutic target in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mengru Liu, Xinyu Zhou, Yue Li, Shijia Ma, Ling Pan, Xingxian Zhang, Wanqing Zheng, Zhanxun Wu, Ke Wang, Anil Ahsan, Jiaying Wu, Lei Jiang, Yangyang Lu, Weiwei Hu, Zhenghong Qin, Zhong Chen, Xiangnan Zhang
Summary: TIGAR induces autophagy to alleviate oxidative stress and activate Nrf2 in prolonged ischemic brains, providing sustainable antioxidant defense.
Article
Biochemistry & Molecular Biology
Xinyi Wang, Haiyang Zhang, Shengjie Yin, Yuchong Yang, Haiou Yang, Jiayu Yang, Zhengyang Zhou, Shuang Li, Guoguang Ying, Yi Ba
Summary: Oxaliplatin is a standard treatment for colorectal cancer, but chemoresistance is a challenge. A study found that a short peptide, pep-AP, can increase cancer cell sensitivity to L-OHP, offering a potential treatment option for L-OHP resistant CRC.
Article
Biochemistry & Molecular Biology
Valentin Jacquier, Delphine Gitenay, Vincent Cavailles, Catherine Teyssier
Summary: This study reveals a novel role for the transcription regulator RIP140 in regulating G6PD levels in breast cancer cells, which affects the proliferation ability of the cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Pei-Yang Gao, Ya-Nan Ou, Yi-Ming Huang, Zhi-Bo Wang, Yan Fu, Ya-Hui Ma, Qiong-Yao Li, Li-Yun Ma, Rui-Ping Cui, Yin-Chu Mi, Lan Tan, Jin-Tai Yu
Summary: Liver function may play a role in the progression of Alzheimer's disease. The study found that as AD progressed, certain liver function markers increased while others decreased. The relationship between liver function and CSF AD biomarkers indicates a potential mediation effect on cognition.
JOURNAL OF NEUROCHEMISTRY
(2024)