Article
Chemistry, Applied
Jingyu Wang, Elif Erdem, John M. Woodley
Summary: Biocatalytic oxidation holds promise for the selective synthesis of active pharmaceutical intermediates. Bubbling air or oxygen is an effective method to enhance oxygen transfer, but it leads to enzyme deactivation. This study investigates the stability of NAD-(P)H oxidase (NOX) at different gas-liquid interfaces and provides insights for improving biocatalyst performance.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2023)
Article
Nanoscience & Nanotechnology
Haili Zhang, Xiao Xu, Dan Yan, Chunlin Ren, Jinghan Zhang, Mengzhen Gu, Yun Wang, Peiye Wu, Zhongrui Li, Lingyi Kong, Chao Han
Summary: Researchers developed a new nanodrug delivery system (PN) to increase ROS production by degrading the NQO1 protein, leading to enhanced anti-tumor effects. In vitro and in vivo studies demonstrated that PN significantly increased ROS production and exhibited potent antitumor properties. This nanoplatform might provide an alternative approach for treating tumors with NQO1 overexpression.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Pharmacology & Pharmacy
Sergejs Zavadskis, Adelheid Weidinger, Dominik Hanetseder, Asmita Banerjee, Cornelia Schneider, Susanne Wolbank, Darja Marolt Presen, Andrey Kozlov
Summary: The study found that the inhibitor DPI has different effects on ROS metabolism and mitochondrial function in various types of cells, such as stem cells and differentiated cells, depending on the cell's energy metabolism. Undifferentiated cells may be a better target for DPI compared to differentiated parenchymal cells.
Review
Nutrition & Dietetics
Jyothi Dhuguru, Ryan W. W. Dellinger, Marie E. E. Migaud
Summary: Dietary vitamin B3 components have been found to increase NAD(+) levels, which decline with age and disease. Supplementation with NAD(+) biosynthetic intermediates, such as NR and NMN, can effectively raise NAD(+) levels. In addition, inhibiting NAD(+)-consuming enzymes or activating biosynthetic pathways is also a viable approach to increase NAD(+) and NADH. However, the pharmacological significance of NAD(H) catabolites is often overlooked, despite some of these metabolites serving as biomarkers in physiological disorders.
Article
Chemistry, Multidisciplinary
Majun Yang, Weida Zhu, Yilin Lv, Bin Jiang, Chenxia Jiang, Xiaobo Zhou, Guo Li, Yuling Qin, Qi Wang, Ziwei Chen, Li Wu
Summary: This study introduces a dual-responsive indicator, DQ1, capable of imaging H2O2 and NAD(P)H, critical indicators of oxidative stress and antioxidant capacity. The experiments demonstrate the effectiveness of DQ1 in detecting changes in H2O2 and NAD(P)H levels in living cells, zebrafish, and mouse models. Furthermore, DQ1 shows potential in monitoring the dynamic alterations in oxidative stress and antioxidant capacity within disease models.
Article
Pharmacology & Pharmacy
Zhiyong Li, Zhilei Wang, Fang Dong, Wei Shi, Wenzhang Dai, Jing Zhao, Qiang Li, Zhi-E Fang, Lutong Ren, Tingting Liu, Ziying Wei, Wenqing Mou, Li Lin, Yan Yang, Xiaohe Xiao, Li Ma, Zhaofang Bai
Summary: Liver fibrosis is characterized by abnormal proliferation of connective tissue in the liver due to various pathogenic factors. The study demonstrates that germacrone (GER) can inhibit liver fibrosis by regulating multiple signaling pathways, including reducing ROS release to protect hepatocytes and inhibiting HSC activation through TGF-beta/Smad and apoptosis pathways. GER shows potential as a therapeutic drug for the treatment of liver fibrosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Plant Sciences
Bixin Bai, Guoding Zhang, Yue Li, Yanbin Wang, Shrestha Sujata, Xudong Zhang, Licheng Wang, Lei Zhao, Yunfeng Wu
Summary: In this study, it was found that the potential effector SWP12 secreted by 'Ca. P. tritici' weakens the basal immunity of host plants and promotes colonization by other pathogens. The WRKY transcription factors in wheat and Japonica Nicotiana benthamiana were identified as host targets of SWP12.
Review
Chemistry, Multidisciplinary
Andreas S. Bommarius
Summary: While many oxidases are active and selective, they often lack stability for chemical synthesis or large-scale processing due to various stresses, such as loss of flavin co-enzyme, overoxidation in the presence of reactive oxygen species, or instability in the presence of air/water interfaces if the co-substrate oxygen is supplied by sparging.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Qi Chen, Jiabao Huang, Weizhao Tong, Xingang Gui, Jie Zheng, Guoxin Hu
Summary: This study investigated the geometric effect of GO nanomaterials on HSCs and found that GO nanosheets reduced cell viability and mobility of HSCs, while increasing the expression level of α-SMA. GO nanosheets disrupted mitochondria membrane and induced oxidative stress and HSCs activation. GO quantum dots decreased α-SMA expression level, and the correlation between GAPDH and α-SMA needs further exploration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Hui Liu, Wei-Li Lu, Hai-Qin Hong, Meng-Jun Li, Man-Ping Ye, Qiu-Fan Rao, Jin-Ling Kong, Shao-Hua Luan, Yan Huang, Qing-Hua Hu, Fan-Rong Wu
Summary: In this study, it was found that ASIC1a activation is involved in the activation and proliferation of HSCs and plays a role in the occurrence and development of liver fibrosis. Inhibition of ASIC1a improved acid-induced HSC activation and the expression of CaM/CaMKII in HSCs regulated by ASIC1a. These findings have important implications for the treatment of liver fibrosis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Physical
Jingyu Wang, John M. Woodley
Summary: This paper investigates the regeneration of important cofactors NAD(P)H and NAD(P) in biocatalytic synthesis for ensuring the economic sustainability of the process. The kinetic stability of a water-forming NAD(P)H oxidase (NOX) for in-situ cofactor regeneration in a bubble column is studied. The results show that NOX exhibits two-stage and single-stage deactivation kinetics, with aggregation and unfolding at the gas-liquid interface being the main cause of deactivation.
Article
Chemistry, Multidisciplinary
Zebin Yang, Yu Luo, Yanan Hu, Kaicheng Liang, Gao He, Qian Chen, Qigang Wang, Hangrong Chen
Summary: By developing novel nanoparticles combined with photothermal and gas therapy, tumor growth can be inhibited in the tumor environment, opening up a new approach for the design of gas-mediated cancer treatment.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Physiology
Tan Yong Chia, Vikneswaran Murugaiyah, Nurzalina Abdul Karim Khans, Munavvar Abdul Sattar, Mohammed Hadi Abdulla, Edward James Johns, Ashfaq Ahmad, Zurina Hassan, Gurjeet Kaur, Ho Yoke Mei, Fiaz Uddin Ahmad, Safia Akhtar
Summary: The study found that treatment with apocynin and catalase in an L-NAME induced hypertension model can attenuate the increase in blood pressure, improve renal function, enhance antioxidative stress capacity, and reduce the expression of Nox 4.
PHYSIOLOGICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Sooyoung Lim, Hee-wang Yoo, Sharad Sarak, Byung-gee Kim, Hyungdon Yun
Summary: The study optimized the enzymatic production of dodecanedioic acid from fatty acids, identified a suitable enzyme for the process, and achieved improved productivity through fusion protein method.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Chemistry, Applied
Marc Biermann, Martin Schuermann, Thomas Schmitges, Andreas Vogel, Jan Brummund
Summary: A biotransformation method to produce (R)-Undecavertol has been developed and successfully demonstrated at the pilot scale with high productivity. By using a highly selective alcohol dehydrogenase and NAD(P)H oxidase, racemic resolution of the substrate is achieved, and oxygen limitation is prevented by the supply of pure oxygen.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2022)
Review
Cell Biology
Yueshui Zhao, Bo Chen, Jing Shen, Lin Wan, Yinxin Zhu, Tao Yi, Zhangang Xiao
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2017)
Article
Cell Biology
Qijie Zhao, Fuyan Hu, Zhangang Xiao, Mingxing Li, Xu Wu, Yueshui Zhao, Yuanlin Wu, Jianhua Yin, Ling Lin, Hanyu Zhang, Lingling Zhang, Chi Hin Cho, Jing Shen
CELL PROLIFERATION
(2018)
Article
Urology & Nephrology
Huihui Huang, Chunhua Xu, Yang Wang, Chenling Meng, Wenjing Liu, Yueshui Zhao, Xiao-Ru Huang, Wenxing You, Bo Feng, Zhi-Hua Zheng, Yu Huang, Hui-Yao Lan, Jinzhong Qin, Yin Xia
KIDNEY INTERNATIONAL
(2018)
Review
Immunology
Yueshui Zhao, Ling Lin, Zhangang Xiao, Mingxing Li, Xu Wu, Wanping Li, Xiaobing Li, Qijie Zhao, Yuanlin Wu, Hanyu Zhang, Jianhua Yin, Lingling Zhang, Chi Hin Cho, Jing Shen
JOURNAL OF IMMUNOLOGY RESEARCH
(2018)
Review
Cell Biology
Yueshui Zhao, Ling Lin, Jing Li, Zhangang Xiao, Bo Chen, Lin Wan, Mingxing Li, Xu Wu, Chi Hin Cho, Jing Shen
CELLULAR IMMUNOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Yueshui Zhao, Xin Li, Li Yang, Kristin Eckel-Mahan, Qingchun Tong, Xue Gu, Mikhail G. Kolonin, Kai Sun
MOLECULAR AND CELLULAR BIOLOGY
(2018)
Review
Pharmacology & Pharmacy
Xu Wu, Wei Hu, Lan Lu, Yueshui Zhao, Yejiang Zhou, Zhangang Xiao, Lin Zhang, Hanyu Zhang, Xiaobing Li, Wanping Li, Shengpeng Wang, Chi Hin Cho, Jing Shen, Mingxing Li
ACTA PHARMACEUTICA SINICA B
(2019)
Article
Oncology
Jing Jin, Xu Wu, Jianhua Yin, Mingxing Li, Jing Shen, Jing Li, Yueshui Zhao, Qijie Zhao, Jingbo Wu, Qinglian Wen, Chi Hin Cho, Tao Yi, Zhangang Xiao, Liping Qu
FRONTIERS IN ONCOLOGY
(2019)
Review
Biochemistry & Molecular Biology
Parham Jabbarzadeh Kaboli, Lingling Zhang, Shixin Xiang, Jing Shen, Mingxing Li, Yueshui Zhao, Xu Wu, Qijie Zhao, Hanyu Zhang, Ling Lin, Jianhua Yin, Yuanlin Wu, Lin Wan, Tao Yi, Xiang Li, Chi Hin Cho, Jing Li, Zhangang Xiao, Qinglian Wen
CURRENT MEDICINAL CHEMISTRY
(2020)
Article
Oncology
Yueshui Zhao, Qijie Zhao, Parham Jabbarzadeh Kaboli, Jing Shen, Mingxin Li, Xu Wu, Jianhua Yin, Hanyu Zhan, Yuanlin Wu, Ling Lin, Lingling Zhang, Lin Wan, Qinglian Wen, Xiang Li, Chi Hin Cho, Tao Yi, Jing Li, Zhangang Xiao
TRANSLATIONAL ONCOLOGY
(2019)
Article
Oncology
Tao Zou, Li Gu, Liqiong Yang, Junsong Wei, Yueshui Zhao, Jing Shen, Mingxing Li, Xu Wu, Fukuan Du, Yu Chen, Yun Ye, Zhangang Xiao, Zhigui Wu
Summary: This study explores the potential targets and mechanisms of alpha-solanine in the treatment of non-small cell lung cancer (NSCLC) through network pharmacology and experimental validation. The findings suggest that alpha-solanine may exert anti-NSCLC effects by regulating the energy metabolism pathway, inhibiting cell proliferation, migration, and invasion, and promoting cell apoptosis.
RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY
(2022)
Review
Biochemistry & Molecular Biology
Jing Li, Xianmei Zhong, Yueshui Zhao, Jing Shen, Chalermchai Pilapong, Zhangang Xiao
Summary: Lung cancer is the second leading cause of cancer-related death globally. Abnormal expression of microRNAs has been found in lung cancer and they play important roles in its initiation and progression. Polyphenols, natural bioactive chemicals synthesized by plants, have shown promising anticancer effects against lung cancer and other types of cancer. Recent studies have revealed that polyphenols exert their anticancer effects by regulating the expression levels of microRNAs in lung cancer. Therefore, targeting microRNAs using polyphenols may provide a novel strategy for the prevention and treatment of lung cancer.
Review
Oncology
Yueshui Zhao, Jian Deng, Shuangfeng Rao, Sipeng Guo, Jing Shen, Fukuan Du, Xu Wu, Yu Chen, Mingxing Li, Meijuan Chen, Xiaobing Li, Wanping Li, Li Gu, Yuhong Sun, Zhuo Zhang, Qinglian Wen, Zhangang Xiao, Jing Li
Summary: Cell-based immunotherapy, especially tumor-infiltrating lymphocytes (TILs) therapy, has emerged as a powerful strategy in solid cancer treatment. This review summarizes the current strategies and challenges in TIL generation, and discusses the clinical trials where TIL therapy is used independently or in combination with other therapies for solid tumor treatment. The limitations, future potential, and directions of TIL therapy for solid tumors are also discussed.
Article
Biology
Xuejun Fan, Zihao Yuan, Yueshui Zhao, Wei Xiong, Hao-Ching Hsiao, Rahmawati Pare, Jianmin Ding, Ahmad Almosa, Kai Sun, Songlin Zhang, Robert E. Jordan, Cheok Song Lee, Zhiqiang An, Ningyan Zhang
Summary: This study reveals that single-hinge cleaved antibodies (scIgGs) in tumors dampened NK cell cytotoxic activities and resulted in aggressive tumor progression. However, this effect can be rescued by targeting the neoepitope in scIgGs with an anti-hinge antibody.
COMMUNICATIONS BIOLOGY
(2022)
Article
Chemistry, Medicinal
Fangshan Chen, Xianmei Zhong, Qian Dai, Kuo Li, Wei Zhang, Jie Wang, Yueshui Zhao, Jing Shen, Zhangang Xiao, Hongyun Xing, Jing Li
Summary: Genetically engineered umbilical cord-MSCs can continuously express and secrete soluble TRAIL, exerting significant inhibitory effects on B-cell acute lymphocytic leukemia cells, which may become a potential therapeutic strategy for B-ALL treatment.