Article
Chemistry, Applied
Asad Hosseini, Soghra Ramezani, Mahnaz Tabibiazar, Maryam Mohammadi, Zahra Golchinfar, Maryam Mahmoudzadeh, Ali Jahanban-Esfahlan
Summary: This study successfully prepared alpha-amylase encapsulated in water in oil emulsion and electrospun fibers. The immobilized enzyme exhibited high activity across a wide range of pHs and temperatures, and showed good storage stability and reusability.
CARBOHYDRATE POLYMERS
(2022)
Article
Food Science & Technology
Vali Azizi, Maryam Mohammadi, Reza Rezaei Mokarram, Mahmood Sowti Khiabani, Hamed Hamishehkar
Summary: In this study, α-amylase was covalently immobilized on magnetic zeolite particles and exhibited good stability and reusability. The optimal pH for immobilized enzymes shifted towards basic conditions, showing enhanced stability under alkaline conditions, which could be applied in industrial production.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rucha P. Desai, Dolly Dave, Sadikhusain A. Suthar, Shivani Shah, Nidhi Ruparelia, Bhavtosh A. Kikani
Summary: The study focused on immobilizing amylase on graphene oxide-magnetite nanoparticles, optimizing concentrations to achieve high loading capacity, and improving enzyme stability and alkalitolerance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biophysics
Ke Wang, Rui Lv, Shiyong Sun, Faqin Dong, Mingxue Liu, Jin Liu, Xiaoqin Nie
Summary: This study demonstrates a novel approach to immobilize enzymes on nanostructured substrates, constructing nanobiocatalysts for enhanced enzymatic performance. The immobilized alpha-amylase showed higher catalytic activity and stability over a wide range of pH values and temperatures, likely due to allosteric modulation. The study lays the foundation for further industrial applications utilizing the phenomenon of allosteric activation of enzymes.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Physical
Mozhgan Razzaghi, Ahmad Homaei, Roohullah Hemmati, Dariush Saberi, Soudabeh Kavousipour
Summary: Cellulose nanocrystals/gold nanoparticle hybrids were synthesized using hydrothermal treatment without toxic chemicals. The immobilized enzyme showed increased optimal temperature and retained higher activity and efficiency under extreme conditions.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Review
Biotechnology & Applied Microbiology
Prayatna Sharma, Krishnendu Mondal, Keshab Chandra Mondal, Nagendra Thakur
Summary: With the development of green chemistry, the use of enzymes in industrial processes as an alternative to traditional chemical catalysts has gained significant attention. Among these enzymes, alpha-amylase stands out due to its important role in starch hydrolysis and biotechnological applications. Various methods have been devised to improve the thermostability of enzymes in response to industrial demand, including protein engineering, directed evolution, and enzyme immobilisation strategies.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ludovica Primitivo, Martina De Angelis, Andrea Necci, Federica Di Pietro, Alessandra Ricelli, Daniela Caschera, Luciano Pilloni, Lorenza Suber, Giuliana Righi
Summary: Silver thiolate nanoclusters were functionalized with a chiral amino alcohol ligand, which exhibited high catalytic efficiency in asymmetric reactions. The resultant nanostructured catalyst can be easily recovered by centrifugation and showed the same catalytic activity as the homogeneous ligand in the addition of nitromethane to aromatic aldehydes. Additionally, it remained efficient after two recycling cycles. To the best of our knowledge, this is the first example of silver nanoclusters used as a support for chiral ligands in heterogeneous phase asymmetric catalysis.
NANOSCALE ADVANCES
(2023)
Article
Chemistry, Multidisciplinary
Attia Hamid, Asma Zafar, Iram Liaqat, Muhammad Sohail Afzal, Liangcai Peng, Muhammad Khawar Rauf, Ikram ul Haq, Asad ur-Rehman, Sikander Ali, Muhammad Nauman Aftab
Summary: The beta-xylanase gene cloned from Thermotoga naphthophila was expressed in E. coli BL21 DE3 and covalently bound to iron oxide magnetic nanoparticles coated with silica. The immobilized beta-xylanase exhibited effective and superior enzymatic activity, stability, and reusability.
Article
Materials Science, Multidisciplinary
Jenet George, Srinidhi Sonai Anand, Upasana Mohanty, Priyanka Saravanan, Rashmi Lenin, Kongkona Saikia, Vinoth Kumar Vaidyanathan
Summary: The utilization of advanced nanoengineered technologies in the production of fructose syrup using inulinase in a single step is an efficient achievement of enzyme technology. The immobilized biocatalyst shows high reusability with up to 25 cycles, and can be separated from the solution in 20 s by an external magnetic field.
Article
Agriculture, Multidisciplinary
Haitham Al-Madhagi, Valantina Yazbik, Wassim Abdelwahed
Summary: In this study, peroxidase enzyme was isolated from melon peel waste and immobilized onto magnetite nanoparticles for efficient bioremediation of phenol pollutants. The immobilized enzyme showed high activity recovery and purity, with a durable and reusable nature, making it suitable for various applications, especially in bioremediation processes.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2023)
Article
Polymer Science
Prajwal Kulal, Preetha B. Krishnappa, Vishalakshi Badalamoole
Summary: A magnetic hydrogel adsorbent material based on gum acacia polysaccharide has been developed using microwave technology, with magnetite nanoparticles trapped in its network structure. The nanocomposite exhibited super-paramagnetic nature, enhanced adsorption capabilities, and excellent reusability characteristics, making it a promising material for wastewater treatment.
Article
Nanoscience & Nanotechnology
Haizhi Zhang, Zhiping Sun, Cunhui Wu, Xinguang Qin, Gang Liu
Summary: The study focused on the immobilization strategy between ZIF-8 and different enzymes. A magnetic amine-functionalized ZIF-8 with hierarchical pores was designed for efficient covalent immobilization of alpha-amylase. The catalytic activity of immobilized alpha-amylase was explored and demonstrated excellent catalytic activity with good stability and reusability.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Alina Gabriela Rusu, Aurica P. Chiriac, Loredana Elena Nita, Vera Balan, Alexandru Mihail Serban, Alexandra Croitoriu
Summary: This study aims to determine the possibility of substituting GA with SA as a crosslinking agent in the process of enzyme immobilization. A comparison between SA- and GA-functionalized magnetic nanoparticles was conducted, and the results showed that the SA-functionalized MNPs had better stability and catalytic performance compared to the GA-modified MNPs. Therefore, they have greater potential as catalytic nanodevices for biomedical purposes.
Article
Chemistry, Multidisciplinary
Karima Salem, Ylenia Jabalera, Jose David Puentes-Pardo, Jesus Vilchez-Garcia, Adel Sayari, Aida Hmida-Sayari, Concepcion Jimenez-Lopez, Massimiliano Perduca
Summary: Enzyme immobilization on magnetic nanoparticles offers reusability, stability, and robust activity under various conditions. In this study, microbial alpha-amylase and xylanase were successfully immobilized on different types of MNPs using optimized protocols, showing potential for bioindustry applications.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Chemistry, Applied
Hamidreza Aghaei, Zahra Mohammadbagheri, Amineh Hemasi, Ameneh Taghizadeh
Summary: This study successfully immobilized alpha-amylase from Bacillus subtilis on three different supports using adsorption and covalent immobilization methods. The immobilized enzymes showed improved thermal and storage stability, higher specific activity, and good catalytic performance in starch hydrolysis compared to free enzymes.
Article
Chemistry, Multidisciplinary
Vivek Anand, Balaji Sadhasivam, Raghavachari Dhamodharan
NEW JOURNAL OF CHEMISTRY
(2018)
Article
Materials Science, Paper & Wood
Anju Ramakrishnan, Kartik Ravishankar, Raghavachari Dhamodharan
Article
Environmental Sciences
Durgadevi Nagarajan, Swarnalatha Venkatanarasimhan
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2019)
Article
Chemistry, Multidisciplinary
Kartik Ravishankar, K. M. Shelly, Abathodharanan Narayanan, Raghavachari Dhamodharan
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2019)
Article
Biochemistry & Molecular Biology
Durgadevi Nagarajan, Swarnalatha Venkatanarasimhan
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Chemistry, Physical
Bhanumathi Nagarajan, C. D. Athrey, Ramachandran Elumalai, Sudakar Chandran, Dhamodharan Raghavachari
Summary: The study focuses on the use of naphthalimide as an auxiliary acceptor in PTz-based dyes for DSSC applications. Two new organic dyes were synthesized with N-alkylated phenothiazine core and electron acceptor incorporation, showing optimal efficiency in DSSCs with iodide/triiodide electrolyte.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2021)
Article
Engineering, Chemical
Sadhasivam Balaji, Raja Venkatesan, Loganathan Mugeeth, Raghavachari Dhamodharan
Summary: Antibacterial nanocomposite films of PBAT incorporated with chloropropyl functionalized POSS nanofiller were prepared, showing enhanced mechanical and barrier properties with increasing filler content. An optimum filler loading of 3 wt% was identified for maximum enhancement in tensile strength, while 1 wt% filler loading was adequate to double the tensile strength.
POLYMER ENGINEERING AND SCIENCE
(2021)
Article
Polymer Science
Prabha Govindaraj, Dhamodharan Raghavachari
Summary: A novel method for synthesizing a macroporous chitosan hydrogel scaffold with partial physical and chemical crosslinking was introduced in this study. The scaffolds showed promising biocompatibility and potential for applications in hemostasis and drug delivery, although the current system has limitations in fabricating microporous chitosan with higher porosity and surface area.
JOURNAL OF POLYMER RESEARCH
(2021)
Article
Polymer Science
Elanchezhian Sangeetha, Abathodharanan Narayanan, Raghavachari Dhamodharan
Summary: A super water-absorbing hydrogel, CHITUR, was prepared through the hydrothermal reaction of a mixture of chitosan, itaconic acid, and urea, showcasing high water absorption performance and water absorption of saltwater. The structure of CHITUR consists mainly of unmodified chitosan and a small fraction of ionotropically cross-linked itaconic acid involving ITUR salt as cross-links, with the morphology being significantly affected by the composition ratios.
Article
Materials Science, Multidisciplinary
Elanchezhian Sangeetha, Richa Sharma, Abathodharanan Narayanan, Sudha Varadaraj, Raghavachari Dhamodharan
Summary: Tough gels and foams based on chitosan were prepared by hydrothermal reaction, and their structures and properties were characterized. The results showed that CHTAUR had macroporous structures and high porosity, which could be transformed into tough gels through water absorption. The foams exhibited good compressive strength and supported cell growth, making them potential materials for biodegradable packaging and tissue engineering applications.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Polymer Science
Shelly Km, Kartik Ravishankar, Dhamodharan Raghavachari
Summary: The flame resistance of poly(vinyl alcohol) (PVOH) is improved by transforming it into a copolymer using dichloroacetic acid (DCAA) through a green chemical process. The resulting copolymer shows excellent solubility in organic solvents while being inherently flame-retardant.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Prabha Govindaraj, Sivanesan Subramanian, Dhamodharan Raghavachari
Summary: A new composite gel material was prepared through hydrothermal reaction, showing sustained release of cinnamaldehyde and excellent antibacterial activity against both Gram-negative and Gram-positive bacteria.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Engineering, Environmental
Gopika Gokulan, Durgadevi Nagarajan, Swarnalatha Venkatanarasimhan, Asha Sathish
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2019)
Article
Engineering, Environmental
Durgadevi Nagarajan, Swarnalatha Venkatanarasimhan
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2019)
Article
Chemistry, Multidisciplinary
Balaji Sadhasivam, Kartik Ravishankar, Rajpreeth Desingh, Rajalakshmi Subramaniyam, Raghavachari Dhamodharan