Article
Biochemistry & Molecular Biology
Ya Zhuo, Valeria L. Robleto, Adriano Marchese
Summary: beta-arrestins are versatile adaptor proteins that regulate various aspects of G protein-coupled receptor signaling. By using APEX-based proximity labeling, potential novel beta-arrestin interacting partners were discovered. Our study demonstrates that beta arr1-APEX can interact with known interacting proteins and label known beta arr1-interacting partners upon agonist stimulation. This study highlights the value of beta arr1-APEX-based proximity labeling in identifying novel players involved in GPCR signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jorg Reichenwallner, Boyuan Liu, Aidin R. Balo, Wei-Lin Ou, Oliver P. Ernst
Summary: Membrane proteins, such as ion channels, transporters, and GPCRs, are crucial in physiological processes but can be challenging to express in large quantities. Recent studies utilizing EPR techniques have expanded experimental accessibility with relatively small amounts of protein. EPR-based techniques offer significant contributions to understanding complex molecular machinery and energetic phenomena essential for biological processes.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Annalisa Pierro, Malte Drescher
Summary: This Feature Article provides an overview of the recent advances in Site-directed Spin Labeling coupled to Electron Paramagnetic Resonance spectroscopy (SDSL-EPR) for studying biomolecules in living cells. The sensitivity, lack of background interference, and versatility of spin-labeling strategies make this approach highly promising for studying biomolecules in physiological environments.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Ekaterina Georgieva, Yanka Karamalakova, Georgi Arabadzhiev, Vasil Atanasov, Rositsa Kostandieva, Mitko Mitev, Vanya Tsoneva, Yovcho Yovchev, Galina Nikolova
Summary: The main factors in COVID-19 pathology are the abnormal generation of free radicals and oxidative stress, which can cause extensive structural changes at the cellular and molecular levels. The concentration of human serum albumin is crucial for maintaining plasma oncotic pressure, nutrient and drug transport, drug therapy effectiveness, and prevention of drug toxicity. Low albumin levels and structural defects in the protein may lead to ineffective therapy, frequent complications, and high mortality rates.
Review
Biochemistry & Molecular Biology
Keyun Huang, Xianyang Fang
Summary: RNAs are essential biomolecules that are closely associated with various cellular processes and human diseases. Investigating the structure, dynamics, and functional connections of RNA using magnetic resonance techniques such as NMR and EPR spectroscopies is crucial for understanding their biological functions and developing RNA-based therapeutics.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Chemistry, Medicinal
Ningning Sun, Kyeong-Man Kim
Summary: The desensitization of G protein-coupled receptors can occur through various mechanisms including phosphorylation-dependent desensitization at the receptor level and downstream mechanisms involving the sequestration of G proteins. These mechanisms involve GRKs, arrestins, and deubiquitinated arrestins to regulate GPCR signaling and prevent G-protein activation. Further studies are needed on the interesting mechanism of arrestin deubiquitination in GPCR desensitization.
ARCHIVES OF PHARMACAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Steven D. E. Fried, Kushani S. K. Hewage, Anna R. Eitel, Audrey Struts, Nipuna Weerasinghe, Suchithranga M. D. C. Perera, Michael F. Brown
Summary: Maltose activates rhodopsin and enters the protein bound to water, facilitating GPCR signaling. This study challenges the understanding of the role of water molecules in GPCR signaling mechanisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Qiuyan Chen, John J. G. Tesmer
Summary: This review explores the interaction between G protein-coupled receptor kinases (GRKs) and arrestins with GPCRs, as well as the significance of biased agonists for pharmacology. The article compares structures to investigate the molecular mechanisms of biased receptor signaling and examines how arrestins interpret the phosphorylation barcodes installed by GRKs in the intracellular loops and tails of GPCRs.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Xin Xu, Nevin A. Lambert, Guangyu Wu
Summary: This study reveals that GPCRs can be actively captured by COPII via specific motifs and direct interaction with COPII components, which in turn affects their export dynamics. This provides important insights into COPII targeting and forward trafficking of nascent GPCRs.
Article
Chemistry, Multidisciplinary
Udeep Chawla, Suchithranga M. D. C. Perera, Steven D. E. Fried, Anna R. Eitel, Blake Mertz, Nipuna Weerasinghe, Michael C. Pitman, Andrey V. Struts, Michael F. Brown
Summary: Visual rhodopsin absorbs light and takes in approximately 80 water molecules to form a solvent-swollen active state, which is essential for activating the photoreceptor and is supported by molecular dynamics simulations. Force-based measurements show that the expansion of rhodopsin occurs through changes in cavity volumes and increased hydration in the active Meta II state. Furthermore, the binding and release of the C-terminal helix of transducin is coupled to hydration changes, potentially influencing visual signal amplification through a dynamic allosteric mechanism involving lipid and water interactions in the catalytic G-protein cycle.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Biochemistry & Molecular Biology
Preethi C. Karnam, Sergey A. Vishnivetskiy, Vsevolod V. Gurevich
Summary: Arrestins are a small family of proteins that bind G protein-coupled receptors (GPCRs) with high affinity to active phosphorylated GPCRs. They must have two sensors, which detect receptor-attached phosphates and the active receptor conformation independently, enabling transition into a high-affinity receptor-binding state. This transition involves a global conformational rearrangement that stabilizes the complex by bringing additional elements of the arrestin molecule in contact with a GPCR.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Biochemistry & Molecular Biology
Nicholas J. Kapolka, Bryan L. Roth
Summary: In this study, Tichy and colleagues designed improved light-activated chimeric GPCRs with increased sensitivity and tunable signaling features, utilizing high-resolution GPCR-G protein complex structures.
Article
Biochemistry & Molecular Biology
Andrey V. Struts, Alexander V. Barmasov, Steven D. E. Fried, Kushani S. K. Hewage, Suchithranga M. D. C. Perera, Michael F. Brown
Summary: This article summarizes and reviews the osmotic stress studies of G-protein-coupled receptor rhodopsin. It is found that water plays an important role in the activation of the receptor, with at least 80 water molecules entering the receptor in the transition to the active state. If water influx is prevented, the functional transition of the receptor is reversed. These findings reveal the phenomenon of solvent swelling in the activation mechanism of rhodopsin, with water acting as an allosteric modulator of function for rhodopsin-like receptors in lipid membranes.
BIOPHYSICAL CHEMISTRY
(2024)
Review
Cell Biology
Haoran Jiang, Daniella Galtes, Jialu Wang, Howard A. Rockman
Summary: This review explores the signaling pathways, dynamic structures, and physiological relevance of the three most important GPCR signaling effectors in the cardiovascular system: heterotrimeric G proteins, GPCR kinases (GRKs), and 8-arrestins. It summarizes their prominent roles in GPCR pharmacology before transitioning into less well-explored areas. The application of new technologies has contributed to an increasing understanding of GPCR structure and downstream effectors.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Emilien Etienne, Annalisa Pierro, Ketty C. Tamburrini, Alessio Bonucci, Elisabetta Mileo, Marlene Martinho, Valerie Belle
Summary: Site-directed spin labeling (SDSL) combined with continuous wave electron paramagnetic resonance (cw EPR) spectroscopy is a powerful technique to study protein dynamics. Numerical simulation using SimLabel, a graphical user interface (GUI) of Matlab, is proposed to analyze SDSL-EPR spectra and provide quantitative information on protein dynamics. Guidelines for simulating X-band cw-EPR spectra of nitroxide labeled proteins at room temperature are presented.
Article
Biochemistry & Molecular Biology
Andras D. Toth, Susanne Prokop, Pal Gyombolai, Peter Varnai, Andras Balla, Vsevolod V. Gurevich, Laszlo Hunyady, Gabor Turu
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Medicine, Research & Experimental
Lu Zhu, Mario Rossi, Yinghong Cui, Regina J. Lee, Wataru Sakamoto, Nicole A. Perry, Nikhil M. Urs, Marc G. Caron, Vsevolod V. Gurevich, Grzegorz Godlewski, George Kunos, Minyong Chen, Wei Chen, Jurgen Wess
JOURNAL OF CLINICAL INVESTIGATION
(2017)
Article
Neurosciences
Lilia Zurkovsky, Katayoun Sedaghat, M. Rafiuddin Ahmed, Vsevolod V. Gurevich, Eugenia V. Gurevich
Article
Multidisciplinary Sciences
Qiuyan Chen, Nicole A. Perry, Sergey A. Vishnivetskiy, Sandra Berndt, Nathaniel C. Gilbert, Ya Zhuo, Prashant K. Singh, Jonas Tholen, Melanie D. Ohi, Eugenia V. Gurevich, Chad A. Brautigam, Candice S. Klug, Vsevolod V. Gurevich, T. M. Iverson
NATURE COMMUNICATIONS
(2017)
Article
Biochemical Research Methods
Shih-Chia Tso, Qiuyan Chen, Sergey A. Vishnivetskiy, Vsevolod V. Gurevich, T. M. Iverson, Chad A. Brautigam
ANALYTICAL BIOCHEMISTRY
(2018)
Article
Cell Biology
Whitney M. Cleghorn, Nada Bulus, Seunghyi Kook, Vsevolod V. Gurevich, Roy Zent, Eugenia V. Gurevich
CELLULAR SIGNALLING
(2018)
Review
Biochemistry & Molecular Biology
Qiuyan Chen, Tina M. Iverson, Vsevolod V. Gurevich
TRENDS IN BIOCHEMICAL SCIENCES
(2018)
Review
Pharmacology & Pharmacy
Vsevolod V. Gurevich, EugeniaV. Gurevich
TRENDS IN PHARMACOLOGICAL SCIENCES
(2018)
Article
Neurosciences
Srimal Samaranayake, Xiufeng Song, Sergey A. Vishnivetskiy, Jeannie Chen, Eugenia V. Gurevich, Vsevolod V. Gurevich
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2018)
Article
Multidisciplinary Sciences
Haonan Zhang, Anna Qiao, Linlin Yang, Ned Van Eps, Klaus S. Frederiksen, Dehua Yang, Antao Dai, Xiaoqing Cai, Hui Zhang, Cuiying Yi, Can Cao, Lingli He, Huaiyu Yang, Jesper Lau, Oliver P. Ernst, Michael A. Hanson, Raymond C. Stevens, Ming-Wei Wang, Steffen Reedtz-Runge, Hualiang Jiang, Qiang Zhao, Beili Wu
Editorial Material
Biochemistry & Molecular Biology
Vsevolod V. Gurevich, Eugenia Gurevich
Meeting Abstract
Ophthalmology
Sergey Vishnivetskiy, Lori S. Sullivan, Sara J. Bowne, Stephen P. Daiger, Eugenia Gurevich, Vsevolod V. Gurevich
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2018)
Meeting Abstract
Ophthalmology
Srimal Aminda Samaranayake, Sergey A. Vishnivetskiy, Kimberly C. Thibeault, Eugenia Gurevich, Vsevolod V. Gurevich
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2018)
Meeting Abstract
Ophthalmology
Vsevolod V. Gurevich, Ned Van Eps, Sergey Vishnivetskiy, Luwi Shamambo, Nicole A. Perry, Wayne L. Hubbell
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2018)
Article
Ophthalmology
Sergey A. Vishnivetskiy, Lori S. Sullivan, Sara J. Bowne, Stephen P. Daiger, Eugenia V. Gurevich, Vsevolod V. Gurevich
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)