Article
Biochemistry & Molecular Biology
Shuhei Yamada, Shuji Mizumoto
Summary: This study characterized the properties of HYAL4 and found that it is a specific CS endoglycosidase. It is ubiquitously expressed in various organs in mice and is involved in the intracellular catabolism of CS.
Article
Biochemistry & Molecular Biology
Ayumu Mubuchi, Shoo Katsumoto, Makoto Tsuboi, Hideaki Ishikawa, Yoshihiro Nomura, Kyohei Higashi, Shinji Miyata
Summary: Chondroitin sulfate and heparan sulfate are sulfated glycosaminoglycan chains with different levels of sulfation and disaccharide compositions. The sulfated glycosaminoglycans from Perna canaliculus show potential as biomaterials for studying the structure-function relationships.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Microbiology
Carla Martin, Helena Ordiales, Francisco Vazquez, Marta Pevida, David Rodriguez, Jesus Merayo, Fernando Vazquez, Beatriz Garcia, Luis M. Quiros
Summary: This study found that cell surface glycosaminoglycans (GAGs) are involved in the adhesion of acne-related bacteria to skin cells, with different bacteria affecting different cell types. Bacterial adherence also led to changes in gene expression in skin cells, affecting the structure of GAG chains.
Review
Polymer Science
Qingchi Wang, Lianli Chi
Summary: This review investigates the structural alterations and roles of GAGs/PGs in various diseases, and discusses their potential in disease diagnosis, monitoring, prognosis, and drug development.
Article
Biochemical Research Methods
Gabor Toth, Domonkos Pal, Simon Sugar, Ilona Kovalszky, Katalin Dezso, Gitta Schlosser, Laszlo Drahos, Lilla Turiak
Summary: This study investigated the content and sulfation pattern of chondroitin sulfate (CS) and heparan sulfate (HS) in human hepatocellular carcinoma and cirrhotic liver tissues, considering the etiology of the diseases. Major differences were observed in the total abundance and sulfation pattern of CS and HS chains, providing important insights into the molecular mechanisms of chronic liver diseases.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Oncology
Htoo Zarni Oo, Zoltan Lohinai, Nastaran Khazamipour, Joey Lo, Gunjan Kumar, Jessica Pihl, Hans Adomat, Noushin Nabavi, Hakhamanesh Behmanesh, Beibei Zhai, Robert Dagil, Swati Choudhary, Tobias Gustavsson, Thomas M. Clausen, Jeffrey D. Esko, Jeffrey W. Allen, Michael A. Thompson, Nhan L. Tran, Judit Moldvay, Balazs Dome, Ali Salanti, Nader Al-Nakouzi, Glen J. Weiss, Mads Daugaard
Summary: Targeting oncofetal chondroitin sulfate (CS) chains can serve as a prognostic and therapeutic strategy for non-small cell lung cancer (NSCLC), with high expression associated with poorer outcomes. Both clinical and preclinical studies demonstrate the potential of oncofetal CS as a actionable prognostic marker and therapeutic target in NSCLC.
Review
Biochemistry & Molecular Biology
Weijiao Zhang, Ruirui Xu, Jiamin Chen, Haibo Xiong, Yang Wang, Bo Pang, Guocheng Du, Zhen Kang
Summary: This review summarizes the advances in biosynthesis of chondroitin sulfate (CS) and its oligosaccharides. The use of cell-free enzyme catalyzed systems and de novo biosynthesis strategies allows for the regulation of CS sulfonated pattern and molecular weight, addressing the limitations of animal-derived CS and enabling the production of CS oligosaccharides with defined structures.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Anastasia V. Strokotova, Elvira V. Grigorieva
Summary: Glucocorticoids are commonly used to treat various diseases but can cause side effects. Recent studies suggest that the toxic effects of glucocorticoids on key extracellular components in the brain may be an underlying mechanism for these side effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Karina Biskup, Caroline Stellmach, Elena Ioana Braicu, Jalid Sehouli, Veronique Blanchard
Summary: The modification of glycosaminoglycans in tissues surrounding malignant cells may contribute to tumor progression and metastasis in various types of cancers. A novel GAG-based score, CS-bio, was proposed as a potential biomarker for primary epithelial ovarian cancer, showing better sensitivity and specificity than the clinically used CA125 in identifying early-stage patients.
Article
Microbiology
Lin Wei, Qingdong Zhang, Danrong Lu, Min Du, Xiangyu Xu, Wenshuang Wang, Yu-Zhong Zhang, Xunyi Yuan, Fuchuan Li
Summary: This study identified two novel chondroitin sulfate/dematan sulfate sulfatases that can hydrolyze sulfate groups, providing insights for the structural and functional studies of chondroitin sulfate/dermatan sulfate.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Qian Liu, Heping Cui, Bertrand Muhoza, Khizar Hayat, Shahzad Hussain, Muhammad Usman Tahir, Xiaoming Zhang, Chi-Tang Ho
Summary: WPI-dextran conjugates were obtained through Maillard reaction for different durations, leading to increased grafting degree and browning index over time. The conjugates showed increased content of alpha-helix, reduced surface hydrophobicity, and increased sulfhydryl content as conjugation progressed, with a decrease in fluorescence intensity. These changes resulted in improved stability of nanoparticles and facilitated a decrease in diameter during heating. Compared to non-conjugated nanoparticles, those from WPI-dextran conjugates exhibited excellent stability against aggregation and dissociation under pH variation and increasing ionic strength.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tomoko Watanabe, Ko Takeda, Keiko Hiemori, Toshikazu Minamisawa, Hiroaki Tateno
Summary: This study revealed that the SARS-CoV-2 spike protein binds to both heparin/HS and chondroitin sulfate E, and analyzed the specificity of each subunit of the S protein. Moreover, chondroitin sulfate E may serve as an alternative attachment factor for HS in SARS-CoV-2 infection.
Article
Biochemistry & Molecular Biology
Melanie Bilong, Parisa Bayat, Matthieu Bourderioux, Murielle Jerome, Alexandre Giuliani, Regis Daniel
Summary: Mammalian hyaluronidases play a role in the catabolism of chondroitin sulfate, producing a wide range of oligosaccharides from CS-A and CS-C. Structural investigation using mass spectrometry reveals selective glycosidic bond cleavage catalyzed by mammalian hyaluronidase, supporting its role in CS metabolism. Additionally, differences in oligosaccharide distribution between CS-A and CS-C digestion mixtures have been observed.
Article
Biochemistry & Molecular Biology
Charlotte B. Spliid, Alejandro Gomez Toledo, Ali Salanti, Jeffrey D. Esko, Thomas Mandel Clausen
Summary: Chondroitin sulfate (CS) and dermatan sulfate (DS) are negatively charged polysaccharides commonly found in animal tissue, playing key roles in health and disease. The study reveals significant variations in the activity and specificity of commercial chondroitinases from different vendors, emphasizing the importance of testing enzyme activity in assessing the content and composition of chondroitin and DSs in cells and tissues.
Review
Biochemistry & Molecular Biology
Sampada P. Mutalik, Stephanie L. Gupton
Summary: Research reveals that axonal pathfinding involves multiple guidance molecules and signaling mechanisms, with dynamic transitions between attractive and repulsive cues. The differential expression of receptors and spatial distribution of guidance cues are key mechanisms diversifying axonal guidance responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)