Article
Biochemistry & Molecular Biology
Catherine Duport, Jean-Paul Madeira, Mahsa Farjad, Beatrice Alpha-Bazin, Jean Armengaud
Summary: The study reveals that under fermentative anoxic conditions, MsrA plays a significant role in antioxidant activities, affecting cell growth, motility, and energy metabolism.
Article
Chemistry, Multidisciplinary
Daria Janiszewska, Michal Zloch, Pawel Pomastowski, Malgorzata Szultka-Mlynska
Summary: This research highlights the importance of tailored culture conditions and incubation periods for accurate identification of spore-forming bacteria. The study emphasizes the critical role of sample preparation methods and demonstrates the interplay between incubation time and spore quantity in bacterial identification. These findings deepen our understanding of spore-forming bacteria identification using MALDI-TOF MS.
Article
Reproductive Biology
Matej Murin, Lucie Nemcova, Alexandra Bartkova, Ahmed Gad, Andrea Lucas-Hahn, Frantisek Strejcek, Radek Prochazka, Jozef Laurincik
Summary: The previous strong support for the statement that fully-grown porcine oocytes are transcriptionally quiescent is now challenged. A new study reveals differences in the transcription profiles between germinal vesicle (GV) and metaphase II (MII) oocytes. The study compares the transcription profiles of oocytes at different stages and identifies differentially transcribed mRNAs that are important for key processes during in vitro maturation.
Article
Multidisciplinary Sciences
Kevin Aumiller, Robert Scheffler, Eric T. Stevens, Zehra T. Guvener, Emily Tung, Anna B. Grimaldo, Hans K. Carlson, Adam M. Deutschbauer, Michiko E. Taga, Maria L. Marco, William B. Ludington
Summary: Lactobacilli and Acetobacter sp. are important bacteria that form communities in natural fermentations. This article describes the development of a chemically-defined medium that supports the growth of both groups of bacteria, facilitating the study of their metabolic interactions.
Article
Biotechnology & Applied Microbiology
Fella Hamitouche, Jean-Charles Gaillard, Philippe Schmitt, Jean Armengaud, Catherine Duport, Luc Dedieu
Summary: The study reveals that the Cys proteome of Bacillus cereus undergoes dynamic changes during growth under fermentative anaerobic conditions, reflecting its growth-phase-dependence. Many reactive Cys residues involved in various cellular processes and metabolic pathways were detected as reduced during the growth process.
Article
Biotechnology & Applied Microbiology
Richard Biener, Thomas Horn, Alexander Komitakis, Ines Schendel, Leon Koenig, Anna Hauenstein, Alina Ludl, Andrea Speidel, Svenja Schmid, Julian Weisser, Max Brossmann, Sofia Kern, Max Kronmueller, Sonja Vierkorn, Lennart Suckow, Arthur Braun
Summary: V. natriegens is a halophilic bacterium with the fastest generation time reported so far. A low-chloride chemically defined medium has been developed to achieve high-cell-density cultivation and prevent corrosion on metal surfaces.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Jung-Whan Chon, Kun-Ho Seo
Summary: A new medium for detection of Bacillus cereus, B. cereus chromogenic agar (BCCA), showed similar or better recoverability and inclusivity/exclusivity compared to conventional and commercial chromogenic media. BCCA also exhibited enhanced levels of recoverability and superior selectivity of B. cereus from ready-to-eat food products, particularly radish sprouts and a sprout mix, making it a useful option for isolation and enumeration of B. cereus from commercial foods.
Article
Biotechnology & Applied Microbiology
Anna M. Kolkmann, Anon Van Essen, Mark J. Post, Panagiota Moutsatsou
Summary: This study aimed to develop a defined, cost-effective serum-free and animal-free medium for the attachment and expansion of bovine myoblasts. A serum-free medium composition was developed based on a comprehensive literature analysis and it supported exponential cell growth up to 97% of the serum-containing golden standard growth medium. This is the first defined serum-free and animal-free medium formulation specific for bovine myoblasts to date.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Josep M. Cambra, Emilio A. Martinez, Heriberto Rodriguez-Martinez, Maria A. Gil, Cristina Cuello
Summary: The study evaluated the transcriptome of in vitro-produced pig embryos cultured in defined and undefined media, showing that culture conditions strongly affect the embryo transcriptome. Although no significant differences were found between in vitro-produced embryos, many altered genes were observed when compared with in vivo-derived embryos.
Article
Ecology
Haibo Chen, Emilie Verplaetse, Tania Jauslin, Pierre Cosson, Leyla Slamti, Didier Lereclus
Summary: This study reveals that amoebas can phagocytose and digest vegetative Bacillus cells, prevent the formation of cysts, and promote spore germination and completion of their developmental cycle in their environment. The interaction between amoebas and Bacillus creates a virtuous circle where each protagonist helps the other to develop.
Article
Biotechnology & Applied Microbiology
Se-Woong Park, Byung-Ha Kang, Hyeong-Min Lee, Sung-Jun Lee, Han-Seul Kim, Hye-Won Choi, Tae Jung Park, Kwang-Hoon Kong
Summary: The optimization of a chemically defined medium for producing secretory recombinant brazilian in Kluyveromyces lactis resulted in higher purity and yield at lower production cost. The expression system showed promising potential for application in the food industry.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Cell Biology
Stefano Panella, Francesco Muoio, Valentin Jossen, Yves Harder, Regine Eibl-Schindler, Tiziano Tallone
Summary: Adipose tissue is a rich source of stem cells, but liposuction cannot provide enough for direct applications in regenerative medicine. Hence, GMP-compliant ex vivo expansion protocols are needed to ensure safe, reproducible, and cost-effective production of cell drugs. The developed UrSuppe cell culture medium allows for the cultivation and differentiation of human adipose stem cells, potentially facilitating the translation of research projects into clinical applications.
Article
Agriculture, Dairy & Animal Science
Yunchao Wa, Chenchen Zhang, Gulin Sun, Hengxian Qu, Dawei Chen, Yujun Huang, Ruixia Gu
Summary: Increasing the concentrations of histidine, isoleucine, and glutamate can significantly enhance the production of free exopolysaccharide (f-EPS) and the viability of Streptococcus thermophilus cells. This increase is achieved by up-regulating the transcription of EPS biosynthesis genes and enhancing the activity of key enzymes involved in sugar nucleotide synthesis. Furthermore, the higher concentrations of histidine, isoleucine, and glutamate result in increased lactose consumption and decreased galactose secretion, indicating the improved production of f-EPS.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Sergio Cortez Ghio, Martin A. Barbier, Emilie J. Doucet, Imad Debbah, Meryem Safoine, Gaetan Le-Bel, Andreanne Cartier, Emilie Jolibois, Amelie Morissette, Danielle Larouche, Julie Fradette, Sylvain L. Guerin, Alain Garnier, Lucie Germain
Summary: We developed a new chemically defined serum-free medium, Surge SFM, which can support massive expansion of human primary keratinocytes and production of skin substitutes. Compared to conventional serum-containing medium, Surge SFM can more efficiently expand keratinocytes and properly form stratified epidermis. It also retains keratin-19-positive epithelial stem cells and maintains similar gene expression as with the conventional medium. Surge SFM can be used to produce bilayered self-assembled skin substitutes histologically similar to those produced using serum-containing medium, which can persist for up to six months when grafted onto mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Baojiang Wu, Yanqiu Wang, Xinhua Wei, Jingcheng Zhang, Jiahui Wu, Guifang Cao, Yong Zhang, Jun Liu, Xihe Li, Siqin Bao
Summary: The overexpression of Nelfa enhances the conversion of mouse ESCs into 2C-like cells and improves somatic cell reprogramming efficiency. Co-overexpression of Nelfa and Bcl2 activates the 2C-like state in ESCs and gives the cells dual cell fate potential. These findings provide insights into the roles of Nelfa and Bcl2 in the establishment and regulation of the totipotent state in mouse ESCs.
CELL PROLIFERATION
(2023)
Article
Biotechnology & Applied Microbiology
Julien Le Lay, Halim Bahloul, Sylvie Serino, Michel Jobin, Philippe Schmitt
Article
Microbiology
Khadidja Senouci-Rezkallah, Michel P. Jobin, Philippe Schmitt
Review
Microbiology
Catherine Duport, Michel Jobin, Philippe Schmitt
FRONTIERS IN MICROBIOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Khadidja Senouci-Rezkallah, Philippe Schmitt, Michel P. Jobin
Article
Biotechnology & Applied Microbiology
Amina Aicha Abbas, Stella Planchon, Michel Jobin, Philippe Schmitt
Article
Food Science & Technology
Frederic Carlin, Julien Brillard, Veronique Broussolle, Thierry Clavel, Catherine Duport, Michel Jobin, Marie-Helene Guinebretiere, Sandrine Auger, Alexei Sorokine, Christophe Nguyen-The
FOOD RESEARCH INTERNATIONAL
(2010)
Article
Microbiology
Severine Thomassin, Michel P. Jobin, Philippe Schmitt
ARCHIVES OF MICROBIOLOGY
(2006)
Article
Food Science & Technology
MP Jobin, T Clavel, F Carlin, P Schmitt
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2002)
Article
Biotechnology & Applied Microbiology
F Morel, F Delmas, MP Jobin, C Diviès, J Guzzo
LETTERS IN APPLIED MICROBIOLOGY
(2001)
Article
Food Science & Technology
J Guzzo, MP Jobin, F Delmas, LC Fortier, D Garmyn, R Tourdot-Maréchal, B Lee, C Divies
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2000)
