Article
Biochemistry & Molecular Biology
Sanghun Kim, Jin-Hyung Lee, Yong-Guy Kim, Yulong Tan, Jintae Lee
Summary: The study found that hydroquinones, especially TBHQ, have inhibitory effects on biofilm formation and virulence factor production by Staphylococcus aureus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Magda Ferreira, Sandra N. Pinto, Frederico Aires-da-Silva, Ana Bettencourt, Sandra I. Aguiar, Maria Manuela Gaspar
Summary: The study evaluated the therapeutic benefits of combining three antibiotics (vancomycin, levofloxacin, and rifabutin) with nanotechnological platforms, with RFB showing the strongest antibacterial effect when incorporated into liposomes. Negatively charged RFB liposomes showed promising potential against biofilm-associated Staphylococcus aureus infections and displayed better performance in in vitro studies. Further in vivo studies are recommended to validate the findings.
Review
Infectious Diseases
Salman Sahab Atshan, Rukman Awang Hamat, Musheer A. Aljaberi, Jung-Sheng Chen, Shih-Wei Huang, Chung-Ying Lin, Benjamin J. Mullins, Anthony Kicic
Summary: The production and use of antibiotics increased significantly after the Second World War, but bacterial resistance has now become a global issue. High-risk individuals, such as burn and wound patients, as well as those with pulmonary infections caused by antibiotic-resistant bacteria, are in urgent need of antibiotics. However, with the decline in investment for new antibiotics, the search for alternative therapeutic strategies, such as bacteriophage therapy, has become crucial.
Article
Pharmacology & Pharmacy
Irfan A. Rather, Mohmmad Younus Wani, Majid Rasool Kamli, Jamal S. M. Sabir, Khalid Rehman Hakeem, Ahmad Firoz, Yong Ha Park, Yan Yan Hor
Summary: In this study, the antimicrobial potential of Lactobacillus metabolite extracts (LMEs) purified from four Lactobacillus strains was investigated against mono- and polymicrobial biofilms formed by C. albicans and S. aureus. The results showed that LMEKAU0021 effectively inhibited biofilm formation and affected the cell membrane integrity in both pathogens.
Article
Microbiology
Ken-ichi Okuda, Satomi Yamada-Ueno, Yutaka Yoshii, Tetsuo Nagano, Takayoshi Okabe, Hirotatsu Kojima, Yoshimitsu Mizunoe, Yuki Kinjo
Summary: This study identified a small compound called JBD1 that has inhibitory activity against Staphylococcus aureus biofilm formation and increases sensitivity to aminoglycosides and respiratory activity. Additionally, JBD1 was found to induce metabolic remodeling in S. aureus. These findings suggest that compounds that enhance respiratory activity may be potential therapeutic agents for chronic S. aureus infections.
Article
Infectious Diseases
Tripti Thapa Gupta, Niraj K. Gupta, Peter Burback, Paul Stoodley
Summary: Research has shown that Staphylococcus aureus can rapidly form free-floating aggregates in synovial fluid, developing biofilm-like resistance to antimicrobial agents. Biofilms formed from aggregates or single planktonic cells showed no significant differences in biomass, surface area, or thickness. However, there were differences in antibiotic susceptibility, with biofilms from single cells showing a 1-log reduction and biofilms from aggregates showing a 2-log reduction when treated with vancomycin for 24 hours.
Article
Agriculture, Dairy & Animal Science
P. A. Pacha, M. A. Munoz, G. Gonzalez-Rocha, I San Martin, M. Quezada-Aguiluz, A. Aguayo-Reyes, H. Bello-Toledo, A. A. Latorre
Summary: This study assessed the strain diversity of 166 Staph. aureus isolates collected from 3 consecutive bulk tank milk samples and from milking equipment in contact with milk from 23 Chilean dairy farms. The analysis revealed highly heterogeneous strain diversity among dairy farms, with certain farms showing a clonal distribution of Staph. aureus strains between milking equipment and bulk tank milk. The findings suggest that Staph. aureus-containing milking equipment may be a potential source of contamination for bulk tank milk, impacting milk quality.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Jin-Hyung Lee, Yong-Guy Kim, Sunyoung Park, Liangbin Hu, Jintae Lee
Summary: Alizarin efficiently inhibits multispecies biofilm formation by acne-causing bacteria without inhibiting bacteria growth. It increases the hydrophilicities of bacterial cells, reduces enzyme production, and inhibits bacterial aggregation. Alizarin also enhances the antibiofilm efficacy when used in combination with antibiotics. Furthermore, it prevents biofilm development by acne-causing bacteria on animal skin.
Article
Biotechnology & Applied Microbiology
Minyoung Kim, Jada S. McMahon, Julianna M. Nerone, Luciana H. Skinner, Guy K. German, Claudia N. H. Marques
Summary: Atopic dermatitis (AD) is characterized by susceptibility to infection and inflammation, as well as skin dysbiosis and ceramide deficiency. The study investigates the impact of AD conditions on S. aureus resilience and finds that the viability of non-dominant bacterial species is affected in both healthy and AD conditions. The results show that S. epidermidis increases S. aureus resilience to sphingosine in biofilms, potentially enhancing the pathogen's recalcitrance in AD skin.
Article
Infectious Diseases
Cristina Velazquez-Suarez, Ruben Cebrian, Carmen Gasca-Capote, Antonio Sorlozano-Puerto, Jose Gutierrez-Fernandez, Manuel Martinez-Bueno, Mercedes Maqueda, Eva Valdivia
Summary: Treating and controlling the spread of MRSA in hospitals is challenging due to their resistance to multiple antibiotics and ability to form biofilms. A bacteriocin called AS-48 was found to be effective against all MRSA strains, with its activity enhanced in the presence of lysozyme. Additionally, AS-48 showed efficacy in reducing the viability of S. aureus biofilms and causing changes in the structure and cell division process of the biofilms.
Review
Chemistry, Multidisciplinary
Dina A. Mosselhy, Mhd Assad, Tarja Sironen, Mady Elbahri
Summary: Staphylococcus aureus is a problematic pathogen with resistant strains like MRSA causing issues in hospitals. Nanoparticles and nanofibers show potential in combating superbugs and biofilms, as well as diagnosing MRSA efficiently. Collaborative efforts and standard testing procedures are recommended to address challenges in nanotheranostics.
Article
Infectious Diseases
Shizhou Wu, Junqi Zhang, Qi Peng, Yunjie Liu, Lei Lei, Hui Zhang
Summary: Antibiotic resistance, particularly in MRSA infections, is a serious global health concern with significant social and financial consequences. The YycFG two-component signal transduction system in Staphylococcus aureus is crucial for bacterial viability and plays a key role in cell membrane metabolism, cell wall synthesis, and biofilm formation. Inhibition of the YycFG pathway shows promise as a potential target for managing MRSA-induced infections by reducing biofilm formation and bacterial pathogenicity.
Article
Agriculture, Dairy & Animal Science
Shamsaldeen Ibrahim Saeed, Khairun Anisa Mat Yazid, Hidayatul Athirah Hashimy, Siti Khadijah Dzulkifli, Fatihah Nordin, Nik Azmi Nik Him, Mohd Fikry Fahmi bin Omar, Erkihun Aklilu, Maizan Mohamad, Che Wan Salma Zalati, Nor Fadhilah Kamaruzzaman
Summary: This study reports the prevalence and characterization of Staphylococcus aureus (S. aureus) isolated from bovine mastitis in dairy farms in East Coast Peninsular Malaysia. The study shows the presence of subclinical mastitis and antimicrobial resistance (AMR) in S. aureus isolated from milk samples. Furthermore, S. aureus isolates demonstrate the ability to invade mammary epithelial cells and form biofilms, presenting challenges to mastitis treatment.
Review
Infectious Diseases
Qi Peng, Xiaohua Tang, Wanyang Dong, Ning Sun, Wenchang Yuan
Summary: Bacteria can form biofilms to protect themselves and become persistent against external challenges. Staphylococcus aureus is a common pathogen in biofilm infections. Understanding the formation and regulation mechanisms of bacterial biofilms may provide insights against antibiotic resistance.
Article
Chemistry, Multidisciplinary
Ting Yang, Shi Yang, Tasdiq Ahmed, Katherine Nguyen, Jinlong Yu, Xuejun Cao, Rui Zan, Xiaonong Zhang, Hao Shen, Meredith E. Fay, Evelyn Kendall Williams, Wilbur A. Lam, Jeremy Scott VanEpps, Shuichi Takayama, Yang Song
Summary: A material similar to NETs, called mu webs, was synthesized to study the antimicrobial activity of NETs against Staphylococcus aureus. It was found that mu webs composed of low-to-intermediate concentrations of DNA-histone complexes successfully trapped and inhibited the growth and biofilm formation of S. aureus.
ADVANCED MATERIALS INTERFACES
(2021)
Article
Chemistry, Organic
Risto Savela, Dieter Vogt, Reko Leino
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2020)
Review
Chemistry, Multidisciplinary
Asiri A. Hettikankanamalage, Robert Lassfolk, Filip S. Ekholm, Reko Leino, David Crich
Article
Biochemistry & Molecular Biology
Miralda Madar Johansson, Eva Belurier, Anastassios C. Papageorgiou, Anders P. Sundin, Jani Rahkila, Teemu Kallonen, Ulf J. Nilsson, Santeri Maatsola, Thomas K. M. Nyholm, Jarmo Kapyla, Jukka Corander, Reko Leino, Jukka Finne, Susann Teneberg, Sauli Haataja
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Robert Lassfolk, Sara Bertuzzi, Ana Arda, Johan Warna, Jesus Jimenez-Barbero, Reko Leino
Summary: The phenomenon of acyl group migration in carbohydrates was studied in a trisaccharide model compound and shown to potentially occur in native polysaccharides as well. The implications of this migration process on cell signaling events and plant growth are intriguing areas for further research.
Article
Chemistry, Multidisciplinary
Matej Zore, Shella Gilbert-Girard, Ines Reigada, Jayendra Z. Patel, Kirsi Savijoki, Adyary Fallarero, Jari Yli-Kauhaluoma
Summary: By synthesizing 28 novel fingolimod derivatives and evaluating their efficacy against various bacteria, it was found that some derivatives demonstrated improved antibacterial activity against Staphylococcus aureus and other Gram-negative bacteria, with no apparent change in cytotoxicity on human cells. Compound 43 and 55, in particular, showed a broader spectrum of antibacterial activity possibly due to structural modifications.
Article
Chemistry, Multidisciplinary
Robert Lassfolk, Manuel Pedron, Tomas Tejero, Pedro Merino, Johan Warna, Reko Leino
Summary: Acyl group migration plays a significant role in the synthesis, isolation, manipulation and purification of acylated organic compounds containing free hydroxyl groups, especially carbohydrates. Our study reveals that the anomeric configuration in monosaccharides, along with the relative configurations of other hydroxyl groups and the properties of the migrating acyl group, greatly influence the migration rate.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Ida Mattsson, Manu Lahtinen, Ruzal Sitdikov, Bianca Wank, Tiina Saloranta-Simell, Reko Leino
Summary: In the last decades, the synthesis and design of low molecular weight organogelators has gained increasing attention. In this work, three potential gelators have been prepared from allylated D-mannose and thoroughly characterized. The results showed that two of the compounds selectively formed gels with hydrocarbon solvents. The most promising gelator compound has the possibility for customizing the gelation properties by further chemical modification or utilizing the alkene group as a linker to increase the mechanical strength of the gel.
CARBOHYDRATE RESEARCH
(2022)
Article
Chemistry, Physical
Gabriele Beltrame, Ida Mattsson, Pia Damlin, Zenghua Han, Carita Kvarnstrom, Reko Leino, Baoru Yang
Summary: This study utilized solid state 13C NMR and FTIR spectroscopy to non-invasively investigate the sterile conk caused by the infection of Inonotus obliquus. The results showed clear distinction between the outer and inner layers of the sterile conk and similarity to wood tissues. The multivariate data analysis also revealed similarity between the sterile conk inner layers and decayed wood and hyphae of I. obliquus, suggesting the need for further studies to revise and clarify the classification of I. obliquus.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Chemistry, Organic
Robert Lassfolk, Manuel Pedron, Tomas Tejero, Pedro Merino, Johan Warna, Reko Leino
Summary: Acetyl groups in oligosaccharides can migrate within one saccharide unit and between different saccharide units. Xyloglucans and xylans are common naturally acetylated polysaccharides in nature, playing important roles in plant cells. Understanding the migration of acetyl groups can help understand the biological activity of polysaccharides.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Robert Lassfolk, Reko Leino
Summary: Acyl group migration has been studied extensively due to its relevance to the synthesis, isolation, and purification of acyl-bearing compounds. It also plays a role in the metabolism of pharmaceuticals. Recent evidence suggests that acyl group migration may occur even over glycosidic bonds, potentially impacting the biological activity of natural polysaccharides in host cells. While previous studies have investigated migration in carbohydrates, comprehensive computational investigations supporting the proposed mechanisms are lacking.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Physical
Ida Mattsson, Johanna Majoinen, Manu Lahtinen, Thomas Sandberg, Anna Fogde, Tiina Saloranta-Simell, Orlando J. Rojas, Olli Ikkala, Reko Leino
Summary: Conformational rigidity plays a crucial role in the self-assembly and liquid crystallinity of block molecules. The stereochemistry of carbohydrate-based assemblies is an important parameter to be considered in material design.
Article
Chemistry, Inorganic & Nuclear
Veronika D. Badazhkova, Risto Savela, Reko Leino
Summary: Selective oxidation of lignin diol model compounds and lignin was achieved using a transfer hydrogenation methodology with a ruthenium catalyst. This method allows for the selective oxidation of benzylic secondary alcohols in model diols and spruce milled wood lignin using a commercially available Shvo catalyst under aerobic conditions. The yields of six ketoalcohols from the model compounds, including lignin monomers with 5-5' and beta-O-4 linkages, ranged from 70-92%. This developed method can be used as an intermediate step to introduce new functional groups into lignin-type structures and lignin for further modifications.
DALTON TRANSACTIONS
(2022)
Article
Chemistry, Multidisciplinary
Robert Lassfolk, Atte Aho, Dmitry Yu Murzin, Reko Leino
Article
Chemistry, Multidisciplinary
Ida Mattsson, Ruzal Sitdikov, Andreas C. M. Gunell, Manu Lahtinen, Tiina Saloranta-Simell, Reko Leino
Article
Chemistry, Physical
Risto Savela, Nataliya D. Shcherban, Marko M. Melander, Igor Bezverkhyy, Irina L. Simakova, Otto Langvik, Ekaterina Kholkina, Tamara Schindler, Annabelle Krauss, Karoliina Honkala, Dmitry Yu Murzin, Reko Leino
Summary: Selective hydrogenation of unsaturated imines over heterogeneous catalysts, specifically with iridium on carbon nitrides and amphoteric metal oxides in toluene solvent, provides the best selectivity towards unsaturated amines. This study demonstrates a promising catalytic performance and chemoselectivity, which has not been reported before, under atmospheric hydrogen pressure and low temperatures.
CATALYSIS SCIENCE & TECHNOLOGY
(2021)
