Article
Microbiology
Shiv Kumar, Rohit Verma, Sandhini Saha, Ashish Kumar Agrahari, Shivangi Shukla, Oinam Ningthemmani Singh, Umang Berry, Tushar Kanti Maiti, Shailendra Asthana, C. T. Ranjith-Kumar, Milan Surjit
Summary: Multiple processes exist in a cell to ensure continuous production of essential proteins through different translation processes. Viruses have evolved strategies to use the host translation machinery for their protein synthesis. This study identifies the RNA-protein interactome of the HEV IRESl element and demonstrates the roles of ribosomal protein RPL5 and RNA helicase A (DHX9) in mediating HEV IRESl activity, establishing it as a bona fide internal translation initiation site.
MICROBIOLOGY SPECTRUM
(2023)
Article
Plant Sciences
Helena Jaramillo-Mesa, Emma Fischer, Aurelie M. Rakotondrafara
Summary: Diverse elements within the 5' untranslated region of mRNA can affect translation efficiency. This study reveals the cooperative role of multiple CU-rich segments flanking the TriMV YX-AUG motif in internal initiation of translation. These auxiliary domains may enhance ribosome capacity and delivery to the correct initiation site.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Riai Okamoto, Nobumasa Ito, Yutaro Ide, Bouchra Kitab, Yoshihiro Sakoda, Kyoko Tsukiyama-Kohara
Summary: This study designed siRNAs targeting CSFV-IRES activity and constructed lentiviral vectors expressing shRNA. The results showed that lentiviral vector expressing sh198 significantly suppressed CSFV infection.
Review
Gastroenterology & Hepatology
Hui-Chun Li, Chee-Hing Yang, Shih-Yen Lo
Summary: HCV, an obligatory intracellular pathogen, relies heavily on host cells for successful propagation. Its lifecycle includes stages such as viral entry, protein translation, RNA replication, viral assembly, and release, with hundreds of cellular factors identified over three decades of research. These factors, some of which are targeted for anti-HCV therapies, provide valuable insight into HCV replication strategies.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Reina Sasaki-Tanaka, Toshikatsu Shibata, Hiroaki Okamoto, Mitsuhiko Moriyama, Tatsuo Kanda
Summary: In this study, several novel therapeutic drugs were investigated through a drug repositioning approach, with favipiravir identified as a potential antiviral against hepatitis A virus (HAV) infection. The study examined the replication of HAV and introduced nucleotide mutations in the HAV genome using different drugs. Favipiravir showed inhibitory effects on HAV replication and introduced more nucleotide mutations compared to ribavirin. Further in vivo experiments are needed to confirm the efficacy of favipiravir in treating severe HAV infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Lenka Stejskal, Mphatso D. Kalemera, Charlotte B. Lewis, Machaela Palor, Lucas Walker, Tina Daviter, William D. Lees, David S. Moss, Myrto Kremyda-Vlachou, Zisis Kozlakidis, Giulia Gallo, Dalan Bailey, William Rosenberg, Christopher J. R. Illingworth, Adrian J. Shepherd, Joe Grove
Summary: This study reveals that the hypervariable region-1 (HVR-1) of the fusion proteins E1E2 in Hepatitis C Virus (HCV) has an autoinhibitory function that controls the activity of the proteins. The mechanism is turned off by host receptor interactions, allowing virus entry. Mutations or deletion of HVR-1 results in enhanced virus entry but increased sensitivity to neutralising antibodies.
Review
Chemistry, Medicinal
Paulo Fernando da Silva Santos Junior, Joao Xavier de Araujo Junior, Edeildo Ferreira da Silva Junior
Summary: Infections caused by the Hepatitis C virus (HCV) have a global impact, affecting 70 million people and leading to serious liver problems. Despite therapeutic advancements, a significant percentage of infected individuals are unable to eliminate the virus, highlighting the need for effective antiviral strategies. Targeting the entry of HCV into host cells has emerged as a promising approach for developing antiviral molecules.
MINI-REVIEWS IN MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Xia Yang, Hailiang Sun, Zhening Zhang, Weixin Ou, Fengxiang Xu, Ling Luo, Yahong Liu, Weisan Chen, Jianxin Chen
Summary: Influenza A virus (IAV) infections pose a serious threat to public health and there is an urgent need for novel anti-IAV medications. This study reveals that ginsenoside rk1 (G-rk1) and G-rg5, derived from panax ginseng, exhibit significant antiviral effects against different IAV subtypes both in vitro and in vivo. G-rk1 specifically blocks IAV binding to sialic acid and interacts with HA1, making it a potential approach for preventing and treating IAV infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yani Arhab, Anna Miscicka, Tatyana Pestova, Christopher U. T. Hellen
Summary: Unlike Picornaviridae members, which have long 5 ' untranslated regions (5 'UTRs) with internal ribosomal entry sites (IRESs), Caliciviridae members typically have short 5 'UTRs and translation initiation is mediated by the interaction between the viral 5 ' terminal genome-linked protein (VPg) and eIF4F subunits instead of IRES. However, it was unexpected to find calicivirus genomes with unusually long 5 'UTRs, prompting a detailed examination. Sequence analysis and structural modeling revealed that these atypically long 5 ' UTRs of Caliciviridae isolates contain picornavirus-like type 2 IRESs, offering evidence for a common evolutionary origin between calicivirus and picornavirus IRESs.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Yingying Li, Guangxiang Luo
Summary: HBV infection is a major global health issue, with current treatments being limited. Recent research has shown that LDLR plays a crucial role in HBV infection, suggesting potential new avenues for HBV therapy. Further studies are needed to explore the involvement of other LDLR family members in HBV infection.
Article
Virology
Leandro Fernandez-Garcia, Jenniffer Angulo, Hade Ramos, Aldo Barrera, Karla Pino, Jorge Vera-Otarola, Marcelo Lopez-Lastra
Summary: The DENV IRES enables viral protein synthesis under conditions that suppress canonical translation initiation, with resistance to eIF4G cleavage and enhanced activity in HEK 293T cells expressing Human rhinovirus 2A protease. This study characterizes the mechanism dependent on an internal ribosome entry site (IRES) for DENV mRNA translation initiation both in vitro and in cells.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Mathew P. Kirby, Ciara Stevenson, Liam J. Worrall, Yihang Chen, Christina Young, Jisoo Youm, Natalie C. J. Strynadka, Douglas W. Allan, Eric Jan
Summary: Viruses rely on internal ribosome entry sites to utilize the host cell machinery for viral protein expression. Mutations in the hinge region of dicistrovirus IRES revealed its significant role in viral translation.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Su Thandar Hlaing, Potjanee Srimanote, Pongsri Tongtawe, Onruedee Khantisitthiporn, Kittirat Glab-ampai, Monrat Chulanetra, Jeeraphong Thanongsaksrikul
Summary: In this study, specific regions of the EV-A71 IRES were identified and used to select a single-chain antibody that binds specifically to the IRES. Molecular docking analysis revealed the specific amino acid residues involved in the interaction between the antibody and the IRES. This antibody has the potential to be used as a tool to study the biology of the EV-A71 RNA genome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Kamilla Toon, Mphatso D. Kalemera, Machaela Palor, Nicola J. Rose, Yasuhiro Takeuchi, Joe Grove, Giada Mattiuzzo
Summary: Due to increased and broadened screening efforts, the number of viral species in the Hepacivirus genus has rapidly expanded. Genetic features of hepaciviruses suggest they have adapted and evolved to hijack host proteins for efficient liver propagation. A study on GB virus B (GBV-B) found that claudin-1 is an essential entry factor, shared with hepatitis C virus (HCV), but with distinct mechanisms of entry. Understanding the molecular mechanisms of hepacivirus entry can inform the design of new vaccines and treatments targeting the first stage of HCV infection.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zuben P. Brown, Irina S. Abaeva, Swastik De, Christopher U. T. Hellen, Tatyana Pestova, Joachim Frank
Summary: This study presents cryo-EM structures of hepatitis C virus IRES initiation complexes, revealing the dynamic network of 40S/IRES contacts and the conformational changes during the transition from eIF2- to eIF5B-containing 48S complexes, providing important insights into the process of subunit joining.
Article
Biochemical Research Methods
Mark A. Boerneke, Thomas Hermann
Editorial Material
Multidisciplinary Sciences
Thomas Hermann
Editorial Material
Biochemistry & Molecular Biology
Mark A. Boerneke, Thomas Hermann
Article
Biochemistry & Molecular Biology
Brian P. Charrette, Mark A. Boerneke, Thomas Hermann
ACS CHEMICAL BIOLOGY
(2016)
Article
Chemistry, Multidisciplinary
Mark A. Boerneke, Sergey M. Dibrov, Thomas Hermann
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2016)
Article
Biotechnology & Applied Microbiology
P. Scott Pine, Sarah A. Munro, Jerod R. Parsons, Jennifer McDaniel, Anne Bergstrom Lucas, Jean Lozach, Timothy G. Myers, Qin Su, Sarah M. Jacobs-Helber, Marc Salit
Article
Cell Biology
Thomas Hermann
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2016)
Article
Biotechnology & Applied Microbiology
P. Scott Pine, Steven P. Lund, Jerod R. Parsons, Lindsay K. Vang, Ashish A. Mahabal, Luca Cinquini, Sean C. Kelly, Heather Kincaid, Daniel J. Crichton, Avrum Spira, Gang Liu, Adam C. Gower, Harvey I. Pass, Chandra Goparaju, Steven M. Dubinett, Kostyantyn Krysan, Sanford A. Stass, Debra Kukuruga, Kendall Van Keuren-Jensen, Amanda Courtright-Lim, Karol L. Thompson, Barry A. Rosenzweig, Lynn Sorbara, Sudhir Srivastava, Marc L. Salit
Article
Multidisciplinary Sciences
Alba Monferrer, Douglas Zhang, Alexander J. Lushnikov, Thomas Hermann
NATURE COMMUNICATIONS
(2019)
Article
Biotechnology & Applied Microbiology
Nike Beaubier, Martin Bontrager, Robert Huether, Catherine Igartua, Denise Lau, Robert Tell, Alexandria M. Bobe, Stephen Bush, Alan L. Chang, Derick C. Hoskinson, Aly A. Khan, Emily Kudalkar, Benjamin D. Leibowitz, Ariane Lozachmeur, Jackson Michuda, Jerod Parsons, Jason F. Perera, Ameen Salahudeen, Kaanan P. Shah, Timothy Taxter, Wei Zhu, Kevin P. White
NATURE BIOTECHNOLOGY
(2019)
Meeting Abstract
Chemistry, Multidisciplinary
Walter Frauman, Thomas Hermann
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2017)
Article
Chemistry, Multidisciplinary
Ashok Nuthanakanti, Mark A. Boerneke, Thomas Hermann, Seergazhi G. Srivatsan
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2017)
Meeting Abstract
Biochemistry & Molecular Biology
Victoria Feher, Michael Gilson, Thomas Hermann
Meeting Abstract
Chemistry, Multidisciplinary
Mark Boerneke, Thomas Hermann
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2016)
Meeting Abstract
Chemistry, Multidisciplinary
Arielle Kanner, Anna Cholewczynski, David Schmit, Urszula Milewicz, Roland Wolkowicz, Mark Boerneke, Thomas Hermann, Mikael Bergdahl
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2015)