Article
Cell Biology
Elma Sakinatus Sajidah, Keesiang Lim, Tomoyoshi Yamano, Goro Nishide, Yujia Qiu, Takeshi Yoshida, Hanbo Wang, Akiko Kobayashi, Masaharu Hazawa, Firli R. P. Dewi, Rikinari Hanayama, Toshio Ando, Richard W. Wong
Summary: Small extracellular vesicles (sEVs) play a crucial role in cell communication and can be used for drug delivery and structural characterization of nanoparticles. The high-speed atomic force microscopy (HS-AFM) can evaluate the structural changes of sEVs under different physicochemical stresses.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Review
Spectroscopy
Aleksandra Jaworska, Kamilla Malek, Andrzej Kudelski
Summary: The pH value in different parts of protoplasm is crucial for cell functions, leading to the necessity of intracellular acid-base studies. This review article focuses on intracellular pH measurements using SERS and fluorescence, discussing the theoretical background, experimental approach, motivations, and future perspectives in this field.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Review
Immunology
Raffaella Morini, Matteo Bizzotto, Fabio Perrucci, Fabia Filipello, Michela Matteoli
Summary: The role of microglia in controlling synapse homeostasis is increasingly recognized by the scientific community, with potential therapeutic implications in conditions like schizophrenia and Alzheimer's Disease. Methodological approaches to investigate microglial synaptic engulfment include various in vitro assays and co-cultures of microglia and neurons, requiring further validation in mice brain for representative in vivo results. This review aims to analyze the technical approaches to studying microglia-mediated phagocytosis of neuronal and synaptic substrates in critical developmental time windows.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
Yanqi Yu, Zihan Zhang, Glenn F. W. Walpole, Yan Yu
Summary: Using engineered RotSensors, we discovered that the degradation kinetics of individual phagosomes are not random, but rather linked to their intracellular motion. Faster and more rotational phagosomes are more likely to fuse with lysosomes, resulting in more efficient cargo degradation. This suggests that the motion of phagosomes plays a crucial role in controlling the progression of cargo degradation.
COMMUNICATIONS BIOLOGY
(2022)
Review
Cell Biology
Johannes Westman, Sergio Grinstein
Summary: The ability of phagosomes to halt microbial growth is closely linked to their ability to acidify their luminal pH, but certain pathogens can survive and replicate inside phagosomes by targeting the pH-regulatory machinery of host cells to survive or escape.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Marius Kroger, Jorg Scheffel, Evgeny A. Shirshin, Johannes Schleusener, Martina C. Meinke, Jurgen Lademann, Marcus Maurer, Maxim E. Darvin
Summary: This study demonstrates a noninvasive method (TPE-FLIM) that can visualize macrophages in human dermis. The method shows high sensitivity and specificity, making it valuable for studying the role of macrophages in health and disease.
Review
Chemistry, Physical
Thomas Doneux
Summary: In situ coupling between electrochemistry and fluorescence microscopy is becoming popular, allowing real-time imaging of electrode processes and offering spatial information that cannot be obtained by purely electrochemical methods. Recent trends in this technique, along with advancements in methodology, are discussed in this article, with a focus on the use of fluorogenic probes.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Article
Optics
Adriaan J. Taal, Changhyuk Lee, Jaebin Choi, Bjorn Hellenkamp, Kenneth L. Shepard
Summary: Implantable image sensors have the potential to revolutionize neuroscience by overcoming limitations in high-resolution imaging. Recent advances in technology have enabled ultra-thin lens-less fluorescence imaging, extending sensing capabilities to include photon arrival time and incident angle. A new implantable neural imager with angular-spectral sensitive pixels has been developed, combining metal-insulator-metal (MIM) Fabry-Perot color filters and diffractive optics to enable independent color sensing while doubling the effective pixel density. This imager utilizes angular-spectral and temporal information to demix and localize multispectral fluorescent targets, demonstrating its potential for in-vivo multifluorescent neural imaging.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Multidisciplinary Sciences
Weronika Gonciarz, Magdalena Chmiela, Bartlomiej Kost, Ewelina Piatczak, Marek Brzezinski
Summary: Encapsulation of Salvia cadmica extracts in stereocomplexed microparticles made of biodegradable polymers can enhance the immune response towards Helicobacter pylori. The extracts improve the phagocytic activity of guinea pig macrophages and neutralize H. pylori-induced production of TNF-alpha and IL-10. Further in vivo studies in guinea pigs infected with H. pylori are recommended to confirm the immunomodulatory effects of the encapsulated extracts.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Analytical
Tao Wu, Ting Jing, Yuqi Lu, Fan Zhang, Pingang He
Summary: A Transwell chamber-based two-layer device combined with scanning electrochemical microscopy (SECM) technology was used for in situ investigation of intercellular signal transduction. The cells in the device were cultured on two layers, with the lower layer for signaling cells and the upper layer for signal-receiving cells. By monitoring the extracellular pH (pHe) and ROS release with SECM, it was found that H+ generated by the signaling cells and a shorter distance between the two cell layers both promoted the release of ROS in the signal-receiving cells, indicating H+ as a signaling molecule of intercellular communication. This SECM-based in situ monitoring strategy provides an effective method to study intercellular signal transduction and explore the underlying mechanism.
ANALYTICAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Metin Yildirim, Agnes-Valencia Weiss, Marc Schneider
Summary: Gelatin is a versatile material with biocompatibility, biodegradability, and low toxicity, making it suitable for pharmaceutical applications. Gelatin nanoparticles (GNPs) have great potential for delivering hydrophilic and macromolecular drugs. The mechanical properties of GNPs, particularly their stiffness, play a significant role in their interaction with biological systems. By loading GNPs with FITC-dextran and using atomic force microscopy (AFM), the Young's modulus of the GNPs was determined. The results showed that the prepared GNPs had different stiffness, ranging from 4.12 MPa to 9.8 MPa. In addition, the compatibility of the GNPs with macrophage cell lines from mouse and human origin was evaluated, demonstrating good cell compatibility and efficient cell uptake, especially for the stiffer GNPs.
Article
Multidisciplinary Sciences
Marco Herrera-Barrera, Renee C. Ryals, Milan Gautam, Antony Jozic, Madeleine Landry, Tetiana Korzun, Mohit Gupta, Chris Acosta, Jonathan Stoddard, Rene Reynaga, Wayne Tschetter, Nick Jacomino, Oleh Taratula, Conroy Sun, Andreas K. Lauer, Martha Neuringer, Gaurav Sahay
Summary: Lipid nanoparticle (LNP)-based mRNA delivery shows promise for treating inherited retinal degenerations. By using a peptide phage display library, we identified promising peptide ligands for targeting photoreceptors (PRs). Dye-conjugated peptides quickly localized to PRs and LNPs decorated with these peptides successfully delivered mRNA to PRs, RPE, and Muller glia in mice and nonhuman primates. This development expands the application of LNP-mRNA therapies for inherited blindness by enabling mRNA delivery to the neural retina.
Article
Chemistry, Analytical
Qiang Xiong, Ranran Song, Tao Wu, Fan Zhang, Pingang He
Summary: This study used SECM technology to monitor the changes in extracellular pH values, finding that different types of cells exhibit different pHe changes after electrical stimulation, which was verified through fluorescent staining.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Julia R. R. Shakirova, Vadim A. A. Baigildin, Anastasia I. I. Solomatina, Reza Babadi Aghakhanpour, Vladimir V. V. Pavlovskiy, Vitaly V. V. Porsev, Sergey P. P. Tunik
Summary: A novel pH-responsive phosphorescent probe based on cyclometalated iridium(III) complex is reported, which is covalently conjugated with a water-soluble block-copolymer to prevent oxygen quenching and improve biocompatibility. The resulting polymeric nanoprobe shows a strong response of phosphorescence lifetime to pH variations in the physiological range. Cellular experiments confirm the applicability of this sensor for monitoring intra- and extracellular pH in cell cultures.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Alaa Tafech, Celine Beaujean, Yves Usson, Angelique Stephanou
Summary: There are two groups of fluorescent probes for pH measurements in biological systems. The first group exhibits a single fluorescence emission peak, while the second group exhibits a dual fluorescence emission peak. This study presents a methodology using multiple wavelengths excitation to evaluate the precision of ratiometric measurements for pH determination in biological samples.
Editorial Material
Biology
Joel A. Swanson, Jason S. King
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2019)
Review
Biology
Joel A. Swanson, Sei Yoshida
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2019)
Article
Microbiology
Michael J. Davis, Shannon Moyer, Elizabeth S. Hoke, Edward Sionov, Katrin D. Mayer-Barber, Dan L. Barber, Hongyi Cai, Lisa Jenkins, Peter J. Walter, Yun C. Chang, Kyung J. Kwon-Chung
Article
Multidisciplinary Sciences
Guy M. Lenk, Young N. Park, Rosemary Lemons, Emma Flynn, Margaret Plank, Christen M. Frei, Michael J. Davis, Brian Gregorka, Joel A. Swanson, Miriam H. Meisler, Jacob O. Kitzman
SCIENTIFIC REPORTS
(2019)
Article
Microbiology
Rhys A. Farrer, Miwha Chang, Michael J. Davis, Lucy van Dorp, Dong-Hoon Yang, Terrance Shea, Thomas R. Sewell, Wieland Meyer, Francois Balloux, Hannah M. Edwards, Duncan Chanda, Geoffrey Kwenda, Mathieu Vanhove, Yun C. Chang, Christina A. Cuomo, Matthew C. Fisher, Kyung J. Kwon-Chung
Article
Multidisciplinary Sciences
John C. Charpentier, Di Chen, Philip E. Lapinski, Jackson Turner, Irina Grigorova, Joel A. Swanson, Philip D. King
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Daniel J. Schneider, Katherine A. Smith, Catrina Latuszek, Carol A. Wilke, Danny M. Lyons, Loka R. Penke, Jennifer M. Speth, Matangi Marthi, Joel A. Swanson, Bethany B. Moore, Adam S. Lauring, Marc Peters-Golden
Article
Cell Biology
Amanda O. Wong, Matangi Marthi, Amanda Haag, Irene A. Owusu, Christiane E. Wobus, Joel A. Swanson
Summary: Macrophages have mechanisms to enhance the integrity of their endolysosomes against damage, termed as inducible renitence. Multiple pathways exist for inducing macrophage resistance to membrane damage, depending on the particular microbial stimulus sensed, as observed in this study.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Shayne E. Quinn, Lu Huang, Jason G. Kerkvliet, Joel A. Swanson, Steve Smith, Adam D. Hoppe, Robert B. Anderson, Natalie W. Thiex, Brandon L. Scott
Summary: This study utilized lattice light sheet microscopy to examine the spatial dynamics of plasma membrane, PI3K activity, and structural differences of various macrophage cell types during macropinocytosis. The researchers found that macropinosomes are formed by shaping actin-rich plasma membrane ruffles into large intracellular organelles in a PI3K-coordinated manner. Multiple ruffling morphologies produce macropinosomes, with the majority forming through collisions of adjacent PI3K-rich ruffles.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Zachary Mendel, Mack B. Reynolds, Basel H. Abuaita, Mary X. O'Riordan, Joel A. Swanson
Summary: This study analyzed the effects of extracellular amino acids on nutrient uptake through macropinocytosis in murine macrophages. The results showed that certain amino acids can suppress growth factor-induced macropinocytosis, which may have consequences for macrophage growth and function.
JOURNAL OF CELL SCIENCE
(2022)
Correction
Multidisciplinary Sciences
Shayne E. Quinn, Lu Huang, Jason G. Kerkvliet, Joel A. Swanson, Steve Smith, Adam D. Hoppe, Robert B. Anderson, Natalie W. Thiex, Brandon L. Scott
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Meiqin Hu, Ping Li, Ce Wang, Xinghua Feng, Qi Geng, Wei Chen, Matangi Marthi, Wenlong Zhang, Chenlang Gao, Whitney Reid, Joel Swanson, Wanlu Du, Richard Hume, Haoxing Xu
Summary: A genetic risk factor for Parkinson's disease, TMEM175, acts as a proton-activated, proton-selective channel on the lysosomal membrane to regulate lysosomal H+ leak. Activation of TMEM175 stops further acidification of lysosomes beyond the normal range. Deficiency of TMEM175 causes lysosomal over-acidification, impaired proteolytic activity, and facilitates alpha-synuclein aggregation.
Editorial Material
Biochemistry & Molecular Biology
Joel A. Swanson
Review
Biochemistry & Molecular Biology
Sei Yoshida, Regina Pacitto, Ken Inoki, Joel Swanson
CELLULAR AND MOLECULAR LIFE SCIENCES
(2018)