Article
Agricultural Engineering
Liangqing Zhang, Jiarong Qiu, Jiahui Deng, Shunming Song, Zelong Hong, Wenlong Jia, Suchang Huang, Xianhai Zeng
Summary: This study synthesized a series of non-precious catalysts through co-precipitation and impregnation methods for the conversion of hemicellulosic biomass into sugar alcohols. The Ni8.98Fe1 @ 1.54SiO(2) catalyst prepared by the impregnation method showed excellent catalytic performance for converting xylose to sugar alcohols in a neutral aqueous solution, and it could also be applied to the direct conversion of hemicellulose. The catalyst with mesoporous properties and proper acidic sites played a vital role in the hydrolysis of hemicellulose into pentose sugars.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Stefan Brott, Ki Hyun Nam, Francois Thomas, Theresa Dutschei, Lukas Reisky, Maike Behrens, Hanna C. C. Grimm, Gurvan Michel, Thomas Schweder, Uwe T. T. Bornscheuer
Summary: Marine algae produce complex polysaccharides that can be degraded by marine heterotrophic bacteria using carbohydrate-active enzymes. The degradation of the red algal polysaccharide porphyran involves oxidative demethylation, catalyzed by cytochrome P450 monooxygenase and its redox partners. Genes encoding zinc-dependent alcohol dehydrogenases (ADHs) were found near the genes for key enzymes in this process. The physiological role of these marine ADHs was investigated and it was found that they are required for the utilization of a specific substrate, G6Me.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Neha Bisht, Vikram Dalal, Lakshmi Tewari
Summary: This study identifies a mutant yeast strain that can efficiently convert glucose/xylose sugars from green biomass into ethanol. Molecular docking and molecular dynamics analysis reveal the binding mechanism of the mutant ADH enzyme with substrate and product, providing insights into the enzymatic conversion process.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Neha Bisht, Vikram Dalal, Lakshmi Tewari
Summary: The study explores a mutant yeast Pichia kudriavzevii BGY1-gamma m as a potential strain for bioconversion of glucose/xylose sugars into ethanol. The mutant strain showed higher ethanol concentration during co-fermentation of glucose/xylose sugars and was identified as ADH-1 type. Molecular docking results revealed the stable interaction of substrate and product with the PkADH mutant, forming a lower energy stable protein-ligand complex.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Biotechnology & Applied Microbiology
Paul A. Adamczyk, Samuel T. Coradetti, John M. Gladden
Summary: R. toruloides is a highly tolerant oleaginous yeast with diverse metabolic capacities. This study presents a refined and extended model of R. toruloides pentose metabolism based on genetic and metabolite data. The identified enzymes responsible for pathway flux provide a better understanding of the complexity of microbial pentose metabolism.
MICROBIAL CELL FACTORIES
(2023)
Article
Food Science & Technology
Yuvaraj Ravikumar, Sirajunnisa Abdul Razack, Lakshmi Narayanan Ponpandian, Guoyan Zhang, Junhua Yun, Jiaqi Huang, Donghun Lee, Xiaolan Li, Yuan Dou, Xianghui Qi
Summary: This article summarizes the potential of using metabolic and genetic engineering methods to improve yeast strains for increased production of D-arabitol. It also highlights the importance of understanding the metabolic pathway that regulates D-arabitol synthesis and optimizing the fermentation process.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Food Science & Technology
Rong Huang, Fangfang Zhang, Xingmin Yan, Yi Qin, Jiao Jiang, Yanlin Liu, Yuyang Song
Summary: This study investigated the characteristics of beta-glucosidase from wine-related yeasts in China and analyzed the enzyme activity under different conditions. The research found that non-Saccharomyces species had higher enzymatic activity and that factors such as sugar, pH, temperature, and ethanol affected the enzyme activity differently. These findings provide insights into the potential for utilizing beta-glucosidase in improving the complexity of wine aroma.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Kan Iwata, Mayumi Maeda, Yutaka Kashiwagi, Kenji Maehashi, Jun Yoshikawa
Summary: Arabitol is gaining attention in the food industry as an alternative sweetener with low-caloric and non-cariogenic characteristics. A new yeast strain, kiy1, was isolated from unpasteurized honey for arabitol production. Glucose was found to be the most suitable substrate for arabitol production, with a maximum arabitol production of 101.4 g/L and a production yield of 0.34 g/g of initial glucose. The yeast strain kiy1 showed promising potential for arabitol production.
Article
Biotechnology & Applied Microbiology
Tai Man Louie, Kailin Louie, Samuel DenHartog, Sridhar Gopishetty, Mani Subramanian, Mark Arnold, Shuvendu Das
Summary: The study introduced a two-stage strategy for producing bio-xylitol from D-xylose using recombinant Pichia pastoris expressing a heterologous xylose reductase gene. The biotransformation was shown to be efficient with high conversion rates, and the whole cells could be reused without loss of activity. This method can be applied to other oxidoreductase reactions, demonstrating its potential for various industrial applications beyond xylitol production.
MICROBIAL CELL FACTORIES
(2021)
Article
Biochemistry & Molecular Biology
Tetsuya Ito, Hisaharu Masaki, Koki Fujita, Hiromi Murakami, Motohiro Shizuma, Taro Kiso, Takaaki Kiryu
Summary: D-Glucaric acid is identified as a potential biopolymer building block, and through genetic manipulation of Pseudogluconobacter saccharoketogenes strains, particularly the mutant Rh47-3, higher yields of D-glucaric acid can be achieved. Key enzymes, such as Ps-ADH, Ps-ALDH, and Ps-GADH, play essential roles in the production of D-glucaric acid, with Ps-GADH impacting the formation of byproduct 2-keto-D-gluconic acid.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Agricultural Engineering
Guoyan Zhang, Hossain M. Zabed, Yingfeng An, Junhua Yun, Jiaqi Huang, Yufei Zhang, Xiaolan Li, Jiangfei Wang, Yuvaraj Ravikumar, Xianghui Qi
Summary: In this study, a lactose-rich dairy byproduct, cheese whey powder, was efficiently converted into three low caloric sweeteners using microbial fermentation technologies. The three rare sugars produced showed potential as commercial products, demonstrating the value of utilizing dairy industry waste.
BIORESOURCE TECHNOLOGY
(2022)
Article
Energy & Fuels
Imen Ben Atitallah, Ioanna Ntaikou, Georgia Antonopoulou, Chedly Bradai, Tahar Mechichi, Gerasimos Lyberatos
Summary: The thermochemical/oxidative pretreatment of date palm fibers was investigated to improve bioethanol production. Sodium hydroxide was found to be the most efficient pretreatment reagent, increasing the yield of cellulose. Co-culture with non-conventional yeast improved ethanol production. Date palm fibers show promise as a feedstock for lignocellulosic ethanol production.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Agricultural Engineering
Xiaolan Li, Yufei Zhang, Hossain M. Zabed, Junhua Yun, Guoyan Zhang, Mei Zhao, Yuvaraj Ravikumar, Xianghui Qi
Summary: Metabolic engineering and process optimization were used to improve the production of D-arabitol in Zygosaccharomyces rouxii. The engineered strain ZR-5A achieved a production of 29.01 g/L, 59.31% higher than the parent strain. With the optimal medium composition and fed-batch fermentation, the strain ZR-5A produced 149.10 g/L of D-arabitol with a productivity of 1.04 g/L/h, the highest reported in Z. rouxii system.
BIORESOURCE TECHNOLOGY
(2023)
Article
Food Science & Technology
Hua Cao, Tuoyu Zhou, Haibo Tang, Pengya Feng, Gohar Ali, Pu Liu, Xiangkai Li
Summary: The genetically modified EcN has shown effectiveness in detoxifying ethanol metabolites in liver disease models, restoring liver function, inhibiting oxidative stress, lipid peroxidation, and inflammation, as well as promoting the recovery of gut microbiota homeostasis.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Biotechnology & Applied Microbiology
Stefano Raimondi, Giorgia Foca, Alessandro Ulrici, Lorenza Destro, Alan Leonardi, Raissa Buzzi, Francesco Candeliere, Maddalena Rossi, Alberto Amaretti
Summary: The yeast Wickerhamomyces anomalus WC 1501 has been shown to be an excellent producer of D-arabitol, with high conversion yield and productivity, using glycerol as a substrate.
MICROBIAL CELL FACTORIES
(2022)