期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 39, 期 12, 页码 1761-1769出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-012-1181-2
关键词
Biotransformation; Linalool; Linalool oxide; Substrate feeding product removal; Hydrophobic adsorber
Linalool oxides are of interest to the flavour industry because of their lavender notes. Corynespora cassiicola DSM 62475 has been identified recently as a production organism because of high stereoselectivity and promising productivities [Mirata et al. (2008) J Agric Food Chem 56(9):3287-3296]. In this work, the stereochemistry of this biotransformation was further investigated. Predominantly (2R)-configured linalool oxide enantiomers were produced from (R)-(-)-linalool. Comparative investigations with racemic linalool suggest that predominantly (2S)-configured derivatives can be expected by using (S)-(+)-configured substrate. Substrate and product inhibited growth even at low concentrations (200 mg l(-1)). To avoid toxic effects and supply sufficient substrates, a substrate feeding product removal (SFPR) system based on hydrophobic adsorbers was established. Applying SFPR, productivity on the shake flask scale was increased from 80 to 490 mg l(-1) day(-1). Process optimisation increased productivity to 920 mg l(-1) day(-1) in a bioreactor with an overall product concentration of 4.600 mg l(-1) linalool oxides.
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