期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 39, 期 3, 页码 495-502出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-011-1042-4
关键词
Corynebacterium crenatum; L-Arginine; Cluster; Overexpression; Fermentation
资金
- High-tech Research and Development Programs of China [2007AA02Z207]
- National Basic Research Program of China [2007CB707804]
- National Natural Science Foundation of China [30970056]
- Program for New Century Excellent Talents in the University [NCET-07-0380, NCET-10-0459]
- Fundamental Research Funds for the Central Universities [JUSRP31001]
- Program of Introducing Talents of Discipline to Universities [111-2-06]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
The genes involved in l-arginine biosynthesis in Corynebacterium crenatum are organized as the argCJBDFRGH cluster like in Corynebacterium glutamicum. However, the argC similar to H cluster of the C. crenatum SYPA 5-5, which is an industrialized l-arginine producer, had a lethal mutation occurring in the ArgR repressor encoding gene. The argC similar to H cluster with an inactive argR was overexpressed in E. coli and C. crenatum. In the recombinant E. coli JM109 enzyme activities were increased, and more l-arginine was found in the supernatants from l-glutamine. When the argC similar to H cluster was overexpressed in C. crenatum under its native promoter Parg, l-arginine production was increased by 24.9%, but the presence of the recombinant plasmid pJC-9039 had a negative effect on cell growth. Surprisingly, the DO value of the recombinant strain dropped gently and stayed at a lower level from 24 h to the end of fermentation. The results demonstrated an increasing utilization of oxygen and the distinct enhancement of unit cell l-arginine yields with the cluster argC similar to H-bearing in C. crenatum SYPA-9039. This study provides a kind of Corynebacteria with improved l-arginine-producing ability and an efficient elevation for producing amino acid. Moreover, the promoter Parg would be used as a valid promoter to express objective genes for metabolic engineering in Corynebacteria.
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