期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 37, 期 11, 页码 1203-1209出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-010-0767-9
关键词
Bioflocculant; Corynebacterium glutamicum; Polygalacturonic acid; Constant-rate feeding; Flocculation; Fermentation
资金
- National Natural Science Foundation of China [30700020]
- Xiamen Science and Technology Committee [3502Z20093006]
- Fujian Provincial Department of Science and Technology, China [2008Y0060]
The constant-rate fed-batch production of the polygalacturonic acid bioflocculant REA-11 was studied. A controlled sucrose-feeding strategy resulted in a slight improvement in biomass and a 7% reduction in flocculating activity compared with the batch process. When fed with a 3 g l(-1) urea solution, the flocculating activity was enhanced to 720 U ml(-1) in 36 h. High cell density (2.12 g l(-1)) and flocculating activity (820 U ml(-1)) were obtained in a 10-l fermentor by feeding with a sucrose-urea solution, with values of nearly two times and 50% higher than those of the batch process, respectively. Moreover, the residual sucrose declined to 2.4 g l(-1), and residual urea decreased to 0.03 g l(-1). Even higher flocculating activity of 920 U ml(-1) and biomass of 3.26 g l(-1) were obtained by feeding with a sucrose-urea solution in a pilot scale fermentation process, indicating the potential industrial utility of this constant-rate feeding strategy in bioflocculant production by Corynebacterium glutamicum.
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