期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 37, 期 3, 页码 263-270出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-009-0669-x
关键词
Corynebacterium glutamicum; L-Valine; Pyruvate dehydrogenase; Metabolome analysis; Pathway analysis
资金
- Fachagentur Nachwachsende Rohstoffe (Agency for Renewable Resources) of the BMVEL-German Federal Ministry of Food, Agriculture and Consumer Protection [04NR003/22000304]
- Evonik Degussa GmbH
l-Valine biosynthesis was analysed by comparing different plasmids in pyruvate-dehydrogenase-deficient Corynebacterium glutamicum strains in order to achieve an optimal production strain. The plasmids contained different combinations of the genes ilvBNCDE encoding for the l-valine forming pathway. It was shown that overexpression of the ilvBN genes encoding acetolactate synthase is obligatory for efficient pyruvate conversion and to prevent l-alanine as a by-product. In contrast to earlier studies, overexpression of ilvE encoding transaminase B is favourable in pyruvate-dehydrogenase-negative strains. Its amplification enhanced l-valine formation and avoided extra- and intracellular accumulation of ketoisovalerate.
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