☆ 4.5 Article

Exploration of geosmin synthase from Streptomyces peucetius ATCC 27952 by deletion of doxorubicin biosynthetic gene cluster

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2009)

期刊

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

卷 36, 期 10, 页码 1257-1265

出版社

SPRINGER HEIDELBERG

DOI: 10.1007/s10295-009-0605-0

关键词

Geosmin; Sesquiterpene synthase; Doxorubicin; Streptomyces peucetius; Overexpression

类别

Biotechnology & Applied Microbiology

资金

Ministry of Health & Welfare, Republic of Korea [0720260]
Korea Health Promotion Institute [0720260] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

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Reagent

摘要

Thorough investigation of Streptomyces peucetius ATCC 27952 genome revealed a sesquiterpene synthase, named spterp13, which encodes a putative protein of 732 amino acids with significant similarity to S. avermitilis MA-4680 (SAV2163, GeoA) and S. coelicolor A3(2) (SCO6073). The proteins encoded by SAV2163 and SCO6073 produce geosmin in the respective strains. However, the spterp13 gene seemed to be silent in S. peucetius. Deletion of the doxorubicin gene cluster from S. peucetius resulted in increased cell growth rate along with detectable production of geosmin. When we over expressed the spterp13 gene in S. peucetius DM07 under the control of an ermE* promoter, 2.4 +/- A 0.4-fold enhanced production of geosmin was observed.

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