期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 36, 期 2, 页码 247-251出版社
OXFORD UNIV PRESS
DOI: 10.1007/s10295-008-0491-x
关键词
KG medium; N-3-oxo-hexanoylhomoserine lactone; Proteobacterium; Quorum sensing; Quorum quenching; Sewage
资金
- Malaysia Toray Science Foundation [MTSF1013-2007A]
- Research University Grants [FR146/2007A, TA048/2008A]
- Vote F research grants from the University of Malaya
A novel chemically defined medium, named KG medium, supplemented with N-3-oxo-hexanoylhomoserine lactone (3-oxo-C6-HSL), an acylhomoserine lactone (AHL) used as signalling molecules in Gram-negative bacterial cell-to-cell communication, as the sole source of carbon and nitrogen, was designed and successfully used for the enrichment and isolation of AHL-degrading bacteria. A 3-oxo-C6-HSL-degrading bacterium, 13sw7, was isolated from sewage after six enrichment transfers in the 3-oxo-C6-HSL-containing KG medium. On the basis of the almost complete 16S ribosomal DNA sequence, isolate 13sw7 was clustered with unculturable beta-proteobacteria. This study indicates that the AHL-containing KG medium is effective in isolating AHL-degrading bacteria, including those previously considered unculturable, from environmental sources. To the best of our knowledge, this is the first documentation of the isolation of an AHL-degrading proteobacterium from sewage.
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