期刊
JOURNAL OF IMMUNOTOXICOLOGY
卷 9, 期 4, 页码 426-438出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/1547691X.2012.664578
关键词
Skin enzymes; organ culture; full-thickness; pro-sensitizer; in vitro; sensitization; LLNA; Langerhans cells; metabolic activation; performance standards
类别
Skin exposure to sensitizing chemicals can induce allergic reactions. Certain chemicals, so called pro-sensitizers, need metabolic activation to become allergenic. Their metabolic activation occurs in skin cells such as keratinocytes or dendritic cells. These cell types are also incorporated into dermal in vitro test systems used to assess the sensitizing potential of chemicals for humans. In vitro systems range from single cell cultures to organotypic multi-cellular reconstructed skin models. Until now, their metabolic competence to unmask sensitizing potential of pro-sensitizers was rarely investigated. This review aims to summarize current information on available skin in vitro models and the relevance of xenobiotic metabolizing enzymes for the activation of pro-sensitizers such as eugenol, 4-allylanisole, and ethylendiamine. Among others, these chemicals are discussed as performance standards to validate new coming in vitro systems for their potential to identify pro-sensitizers.
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