Article
Chemistry, Physical
Valentina Wieser, Laura L. E. Mears, Robert D. Barker, Hsiu-Wei Cheng, Markus Valtiner
Summary: This study directly measured lipid bilayer interactions during charge modulation and found that surface potential dependent surface forces due to cation adsorption at high salt concentrations can be effectively described by exponential hydration layer interactions. The results show that the electric double layers of lipid bilayers are mainly regulated by inner Helmholtz charge under physiological conditions.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Patricia Himmels, Thi Thu Thao Nguyen, Maresa Caunt Mitzner, Alfonso Arrazate, Stacey Yeung, Jeremy Burton, Robyn Clark, Klara Totpal, Raj Jesudason, Angela Yang, Margaret Solon, Jeffrey Eastham, Zora Modrusan, Joshua D. Webster, Amy A. Lo, Robert Piskol, Weilan Ye
Summary: Preclinical and clinical studies show that T cell-dependent bispecific antibodies (TDBs) not only kill tumors but also cause systemic changes, leading to adverse events. In this study, the acute responses to TDBs in tumor-bearing mice were characterized in detail. The results reveal rapid and significant accumulation of lymphocytes and activation of endothelial cells (ECs) around large blood vessels in normal organs, particularly the liver. It is suggested that differential responses in normal tissues and tumors may be attributed to organ-specific ECs, and a list of genes selectively upregulated in large liver vessels by TDBs is identified. Furthermore, the study demonstrates that CD9 facilitates the interaction between T cells and ECs through the support of ICAM-1 in response to soluble factors released from TDB-mediated cytotoxic reactions. These findings provide insights into the response of different vascular beds to cancer immunotherapy and may contribute to improving their safety and efficacy.
Review
Biochemistry & Molecular Biology
Wojciech Bialek, James F. Collawn, Rafal Bartoszewski
Summary: This article discusses the role of ubiquitin-mediated protein destabilization in protein degradation, as well as the significance of the ubiquitin-independent pathway and 20S proteasome. It also explores the interaction between the ubiquitin-proteasome system and pathogenic microorganisms, and how these microorganisms manipulate the system to evade or counteract host responses.
Article
Biochemistry & Molecular Biology
Natasha Buwa, Nivedhika Kannan, Shaunak Kanade, Nagaraj Balasubramanian
Summary: Cell-matrix adhesion regulates Cav1 phosphorylation through FAK at focal adhesions, with FAK more prominently modulating pY14Cav1 levels at higher stiffness.
Article
Multidisciplinary Sciences
Jintae Park, Jinwoo Park, Jinhoon Lee, Chanoong Lim, Dong Woog Lee
Summary: In this study, the interaction energies between cyclodextrin-modified surfaces and ditopic adamantane molecules in water were directly quantified using a surface forces apparatus. The adhesion energy of the beta-CD-DAd complex was found to increase significantly with increasing DAd concentration and reached saturation. The molecular adhesion energy of a single host-guest inclusion complex was evaluated to be approximately 9.51 k(B)T. This research provides fundamental information for further understanding of supramolecular chemistry and its applications.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Emma L. Lewis, Rong Xu, Jean-Christophe Beltra, Shin Foong Ngiow, Jordana Cohen, Rahul Telange, Alexander Crane, Deirdre Sawinski, E. John Wherry, Paige M. Porrett
Summary: Studies demonstrate that pregnancy induces exhausted CD8 T cells in mothers, prolonging allograft survival. Maternal CD8 T cells during pregnancy share exhaustion features with CD8 T cells from chronic infection and cancer.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Zahra Mehrazad Saber, Yoshinori Takeuchi, Yoshikazu Sawada, Yuichi Aita, Man Hei Ho, Samia Karkoutly, Duhan Tao, Kyoka Katabami, Chen Ye, Yuki Murayama, Akito Shikama, Yukari Masuda, Yoshihiko Izumida, Takafumi Miyamoto, Takashi Matsuzaka, Takehito Sugasawa, Kazuhiro Takekoshi, Yasushi Kawakami, Hitoshi Shimano, Naoya Yahagi
Summary: This study highlights the role of high protein diet (HPD) in regulating gene expression related to amino acid metabolism in the liver, with Kruppel-like factor 15 (KLF15) playing a key transcriptional regulatory role. Through experiments with knockout mice and analytical systems, new target genes of KLF15 and KLF15-independent genes responsive to HPD were identified, providing insights into the transcriptional regulatory mechanisms of amino acid metabolic pathways.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Mohamed Chamlali, Sana Kouba, Lise Rodat-Despoix, Luca Matteo Todesca, Zoltan Pethoe, Albrecht Schwab, Halima Ouadid-Ahidouch
Summary: Orai3 regulates breast cancer cell migration through a Ca2+-dependent modulation of cell adhesive capacities and a Ca2+-independent regulation of the actin cytoskeleton via focal adhesion kinase (FAK).
Article
Oncology
Xueyun Huo, Wenjing Zhang, Guannan Zhao, Zhenwen Chen, Peixin Dong, Hidemichi Watari, Ramesh Narayanan, Todd D. Tillmanns, Lawrence M. Pfeffer, Junming Yue
Summary: FAK PROTAC effectively inhibits both FAK kinase activity and its scaffold protein activity and shows high effectiveness in inhibiting ovarian tumor growth and metastasis.
FRONTIERS IN ONCOLOGY
(2022)
Article
Plant Sciences
Nan Li, Mika Teranishi, Hiroko Yamaguchi, Tomonao Matsushita, Masaaki K. Watahiki, Tomohiko Tsuge, Shao-Shan Li, Jun Hidema
Summary: Plants have evolved various mechanisms to protect against the harmful effects of UV-B radiation, and CPD photolyase plays a crucial role in this process. In this study, it was found that Arabidopsis regulates the expression of CPD photolyase through multiple photoreceptor signaling pathways, including UVR8-dependent and -independent pathways.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Alistair Noble, Lydia Durant, Stella M. Dilke, Ripple Man, Isabel Martin, Roshani Patel, Lesley Hoyles, Edward T. Pring, Andrew Latchford, Susan K. Clark, Simon R. Carding, Stella C. Knight
Summary: This study aimed to investigate the interaction between the mucosal immune system and commensal bacteria in FAP patients, and found that patients exhibited impaired cellular immunity and reduced tumor surveillance.
CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY
(2022)
Article
Chemistry, Multidisciplinary
Minjung Son, Raymundo Moya, Alberta Pinnola, Roberto Bassi, Gabriela S. Schlau-Cohen
Summary: Plants utilize photoprotection to dissipate excess energy from the sun in order to prevent the generation of harmful photoproducts. Factors such as array size, pH drop, and the presence of carotenoids influence the level of dissipation, providing separate control mechanisms for photoprotection. Understanding these factors allows for a flexible and tunable response to fluctuations in solar energy.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biology
Marion Mathelie-Guinlet, Felipe Viela, Jerome Dehullu, Sviatlana Filimonava, Jason M. Rauceo, Peter N. Lipke, Yves F. Dufrene
Summary: This study investigates the strength of bonds between a- and alpha-agglutinins in budding yeast during mating, revealing insights into the complex mechanism of cell aggregation. Prolonged cell-cell contact and mechanical tension were found to enhance adhesion between mating cells, pointing to potential conformational changes in agglutinins under physical stress.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Amardeep Kumar, Xinhang Yang, Jianbin Li, Jared C. C. Lewis
Summary: The corrinoid protein MtaC, originally involved in methyl transferase catalysis, can catalyze N-alkylation of aniline using ethyl diazoacetate. The study demonstrates how the specific reactivity of B-12 scaffolds for radical versus polar chemistry extends to non-native catalysis, providing guidance for selecting B-12-dependent proteins for biocatalysis. MtaC also exhibits high thermal stability and organic solvent tolerance, remaining folded even in pure methanol.
CHEMICAL COMMUNICATIONS
(2023)
Article
Neurosciences
Michelle R. Emond, Sayantanee Biswas, Matthew L. Morrow, James D. Jontes
Summary: This study investigated the role of Pcdh19 in nervous system development, revealing interactions with proteins that regulate Rho family GTPases, microtubule binding proteins, and cell divisions. These findings provide important insights for future investigations into the cellular and molecular biology of Pcdh19.
Article
Dermatology
Suiting Ao, Xuemei Gao, Jipang Zhan, Lu Ai, Minyi Li, Huilin Su, Xuhua Tang, Coco Chu, Jiande Han, Fang Wang
Summary: The combination of TNF-alpha inhibition and steroids showed better outcomes in treating SJS/TEN compared to steroid monotherapy, significantly shortening the duration of acute phase, hospitalization stay, and skin reepithelialization time. IL-15 was positively correlated with skin reepithelialization time in both groups, while IL-6 served as an additional marker for disease progression in the combination therapy group.
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2022)
Article
Immunology
Lei Zhou, Wenqing Zhou, Ann M. Joseph, Coco Chu, Gregory G. Putzel, Beibei Fang, Fei Teng, Mengze Lyu, Hiroshi Yano, Katrin I. Andreasson, Eisuke Mekada, Gerard Eberl, Gregory F. Sonnenberg
Summary: Group 3 innate lymphoid cells (ILC3s) protect the intestine from TNF-induced cell death by producing heparin-binding epidermal growth factor-like growth factor (HB-EGF), thereby playing a crucial role in inflammatory bowel disease (IBD).
Article
Biochemistry & Molecular Biology
Ying Liu, Bo Ding, Lina Zheng, Ping Xu, Zhiheng Liu, Zhao Chen, Peiyao Wu, Ying Zhao, Qian Pan, Yu Guo, Wei Wang, Wensheng Wei
Summary: Increasing evidence suggests that promoters and enhancers are closely associated with the 3D chromatin structure and have an impact on cellular functions. This study investigated the role of active promoters/enhancers that are predicted to form multiple 3D contacts with other active promoters/enhancers. The findings showed that these hotspots are essential for cell growth and survival, and their deletions result in changes in broad chromatin organization and the expression of distal genes. The essentiality of these hotspots is attributed to their association with multiple dysregulated non-essential genes that collectively affect cell fitness.
NUCLEIC ACIDS RESEARCH
(2022)
Editorial Material
Biology
Wensheng Wei, Caixia Gao
SCIENCE CHINA-LIFE SCIENCES
(2022)
Review
Biology
Guanglei Li, Xiangyang Li, Songkuan Zhuang, Liren Wang, Yifan Zhu, Yangcan Chen, Wen Sun, Zeguang Wu, Zhuo Zhou, Jia Chen, Xingxu Huang, Jin Wang, Dali Li, Wei Li, Haoyi Wang, Wensheng Wei
Summary: The steady progress in genome editing has provided enormous opportunities for biomedical research and human health. It has yielded significant advances in studying human diseases and offers unprecedented capabilities in clinical diagnosis, prevention, and treatment.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Wenqing Zhou, Lei Zhou, Jordan Zhou, Coco Chu, Chao Zhang, Robbyn E. Sockolow, Gerard Eberl, Gregory F. Sonnenberg
Summary: This study defines ROR gamma t(+) immune cells in the intestine at single-cell resolution and identifies a subset of ILC3s that expresses ZBTB46, a transcription factor shared with conventional dendritic cells. ZBTB46 restrains the inflammatory properties of ILC3s and plays a non-redundant role in orchestrating intestinal health.
Article
Biochemistry & Molecular Biology
Wen Zhang, Mengze Lyu, Nicholas J. Bessman, Zili Xie, Mohammad Arifuzzaman, Hiroshi Yano, Christopher N. Parkhurst, Coco Chu, Lei Zhou, Gregory G. Putzel, Ting-Ting Li, Wen-Bing Jin, Jordan Zhou, Hongzhen Hu, Amy M. Tsou, Chun-Jun Guo, David Artis
Summary: Research has found that nociceptive neurons have a significant impact on intestinal inflammation. Disrupted nociception leads to more severe inflammation and defective tissue repair processes. This disruption also causes changes in the intestinal microbiota and dysbiosis, while certain bacteria can promote tissue protection. Additionally, abnormal nociceptor gene expression was observed in intestinal biopsies from patients with inflammatory bowel diseases.
Article
Cell Biology
Xiao-Yu Jiang, Fei-Fei Guan, Jia-Xin Ma, Wei Dong, Xiao-Long Qi, Xu Zhang, Wei Chen, Shan Gao, Xiang Gao, Shuo Pan, Ji-Zheng Wang, Yuan-Wu Ma, Lian-Feng Zhang, Dan Lu
Summary: When pathological hypertrophy progresses to heart failure, the prognosis is often very poor. A study using Trim44 knockout rats generated through CRISPR-Cas9 technology found that Trim44 deficiency protected against cardiac pathological changes and reduced myocardial fibrosis in response to isoproterenol treatment. The study also demonstrated that Trim44 deficiency inhibited signaling pathways involved in myocardial hypertrophy, suggesting it as a potential therapeutic target for preventing cardiac hypertrophy and heart failure.
DISEASE MODELS & MECHANISMS
(2023)
Article
Multidisciplinary Sciences
Mohammad Arifuzzaman, Tae Hyung Won, Ting-Ting Li, Hiroshi Yano, Sreehaas Digumarthi, Andrea F. Heras, Wen Zhang, Christopher N. Parkhurst, Sanchita Kashyap, Wen-Bing Jin, Gregory Garbes Putzel, Amy M. Tsou, Coco Chu, Qianru Wei, Alex Grier, Stefan Worgall, Chun-Jun Guo, Frank C. Schroeder, David Artis
Summary: Dietary fibre, particularly inulin, alters the composition of the microbiota and levels of microbiota-derived metabolites, such as bile acids, leading to type 2 inflammation in the intestine and lungs. This study provides insights into the pathophysiology of allergic inflammation, tissue protection, and host defense.
Article
Multidisciplinary Sciences
Katja J. Jarick, Patrycja M. Topczewska, Manuel O. Jakob, Hiroshi Yano, Mohammad Arifuzzaman, Xuemei Gao, Sotiria Boulekou, Vladislava Stokic-Trtica, Pierre S. Leclere, Alexandra Preusser, Zoe A. Rompe, Anton Stamm, Amy M. Tsou, Coco Chu, Frederik R. Heinrich, Gabriela M. Guerra, Pawel Durek, Andranik Ivanov, Dieter Beule, Sofia Helfrich, Claudia U. Duerr, Anja A. Kuehl, Christina Stehle, Chiara Romagnani, Mir-Farzin Mashreghi, Andreas Diefenbach, David Artis, Christoph S. N. Klose
Summary: This article investigates the role of innate lymphoid cells (ILCs) in protective immunity. Using a mouse model specific to ILCs, researchers found that ILC2s have non-redundant functions during steady state and disease, and they interact with adaptive immune cells to organize the immune system.
Article
Immunology
Xin Liu, Jiacheng Yao, Yongshan Zhao, Jianbin Wang, Hai Qi
Summary: Durable antibody immunity relies on long-lived plasma cells, and new research methods have revealed the phenotypes, heterogeneity, and development pathways of these cells.
Review
Neurosciences
Zhiwei You, Bo Liu, Hai Qi
Summary: The brain has the ability to influence the operation of the immune system, specifically in regards to antibody response and antibody-mediated immunity. The brain-spleen axis plays a crucial role in regulating antigen-specific B cell responses.
Article
Immunology
Xing Zhou, Jiacheng Wei, Liang Li, Zhenfeng Shu, Ling You, Yang Liu, Ruozhu Zhao, Jiacheng Yao, Jianbin Wang, Minmin Luo, Yousheng Shu, Kexin Yuan, Hai Qi
Summary: Abnormalities in microglia, especially the loss of PTEN, can contribute to neurodevelopmental disorders such as autism spectrum disorders. In this study, the deletion of PTEN in microglia in mice led to deficits in sociability and object recognition. Mutant mice showed an increased number of activated microglia with enhanced phagocytosis. Interestingly, delaying the PTEN deletion in microglia for two weeks did not result in social interaction defects, although the mutant microglia remained abnormal in adult animals. The disturbed neurodevelopment caused by early PTEN deletion in microglia was characterized by decreased VGLUT1 protein in synaptosomes, which may be a consequence of enhanced removal by microglia. In vitro recordings also demonstrated weakened synaptic inputs in the developing cortex. Therefore, microglial PTEN plays a crucial role in maintaining the integrity of neural substrates underlying sociability during development.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zongyi Yi, Xiaoxue Zhang, Wei Tang, Ying Yu, Xiaoxu Wei, Xue Zhang, Wensheng Wei
Summary: In this study, researchers propose mitochondrial DNA base editors (mitoBEs), which combine a transcription activator-like effector (TALE)-fused nickase and a deaminase for precise base editing in mitochondrial DNA. By combining mitochondria-localized, programmable TALE binding proteins with the nickase MutH or Nt.BspD6I(C) and either the single-stranded DNA-specific adenine deaminase TadA8e or the cytosine deaminase ABOBEC1 and UGI, they achieve A-to-G or C-to-T base editing with high efficiency and specificity. Furthermore, they demonstrate the potential of mitoBEs in correcting pathogenic mitochondrial DNA mutations in patient-derived cells by delivering mitoBEs encoded in circular RNAs.
NATURE BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhengguang Li, Xiaolong Qi, Xu Zhang, Lei Yu, Lijuan Gao, Weining Kong, Wei Chen, Wei Dong, Lijun Luo, Dan Lu, Lianfeng Zhang, Yuanwu Ma
Summary: This study demonstrates that Trdmt1 plays a protective role in inflammation by regulating the TLR4-NF-kappa B/MAPK-TNF-alpha pathway.
ANIMAL MODELS AND EXPERIMENTAL MEDICINE
(2022)