Article
Cell Biology
Amanda F. Alexander, Ilana Kelsey, Hannah Forbes, Kathryn Miller-Jensen
Summary: IL-10 plays a key role in the inflammatory response induced by TLR4 by regulating cytokine secretion patterns among cells.
Article
Multidisciplinary Sciences
Giorgia Colombo, Cristina Travelli, Chiara Porta, Armando A. Genazzani
Summary: This article investigates the effects of eNAMPT on macrophage-driven inflammation. The study finds that eNAMPT triggers an M1-skewed transcriptional program and selectively boosts IFN gamma-driven transcriptional activation via STAT1/3 phosphorylation. Furthermore, the secretion of eNAMPT promotes the chemotactic recruitment of myeloid cells, creating a positive feedback loop for inflammation. The research also reveals the involvement of receptors other than the recognized TLR4 receptor.
Article
Oncology
Stefano Mangiola, Patrick McCoy, Martin Modrak, Fernando Souza-Fonseca-Guimaraes, Daniel Blashki, Ryan Stuchbery, Simon P. Keam, Michael Kerger, Ken Chow, Chayanica Nasa, Melanie Le Page, Natalie Lister, Simon Monard, Justin Peters, Phil Dundee, Scott G. Williams, Anthony J. Costello, Paul J. Neeson, Bhupinder Pal, Nicholas D. Huntington, Niall M. Corcoran, Anthony T. Papenfuss, Christopher M. Hovens
Summary: This study reveals the important role of monocytes and macrophages in the progression and recurrence of prostate cancer through analysis of the tumor microenvironment. The findings were validated by spatial analyses at the single-cell level using multiplex immunohistochemistry. This study advances our understanding of the immune modulation within the prostate microenvironment and their impact on disease progression and patient survival.
Article
Multidisciplinary Sciences
Karippadakam Bhavana, Dustin J. Foote, Krishnamoorthy Srikanth, Christopher N. Balakrishnan, Vandana R. Prabhu, Shanmugam Sankaralingam, Hijam Surachandra Singha, Achamveetil Gopalakrishnan, Muniyandi Nagarajan
Summary: This study compared the ovarian transcriptome of high egg laying (HEL) and low egg laying (LEL) ducks to identify the genes involved in egg production. They found differentially expressed genes related to glutamate receptor activity, immune function, progesterone mediated oocyte maturation, and other functions.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Jeong Seok Lee, June-Young Koh, Kijong Yi, Young-Il Kim, Su-Jin Park, Eun-Ha Kim, Se-Mi Kim, Sung Ho Park, Young Seok Ju, Young Ki Choi, Su-Hyung Park
Summary: The study utilized a longitudinal approach to analyze single-cell RNA sequencing data from bronchoalveolar lavage fluid cells of SARS-CoV-2-infected ferrets, showing changes in immune response characteristics at 2 and 5 days post-infection compared to uninfected controls. This analysis identified distinct macrophage subsets and gene expression changes associated with SARS-CoV-2 infection, shedding light on the dynamic immune response dynamics triggered by the virus.
NATURE COMMUNICATIONS
(2021)
Article
Neurosciences
Leticia Tavares-Gomes, Camille Monney, Geraldine Neuhaus, David Francisco, Diana Solis, Artur Summerfield, Daniel Erny, Vidhya Jagannathan, Anna Oevermann
Summary: Microglia and MDM play different roles in neuroinfections, with potential data from studies on bovine neuroinfections being applicable to human infections.
Article
Biochemistry & Molecular Biology
Hao Kan, Ka Zhang, Aiqin Mao, Li Geng, Mengru Gao, Lei Feng, Qingjun You, Xin Ma
Summary: This study utilized single-cell RNA sequencing to analyze aortic cells from mice fed with normal or high-fat diets, identifying changes in cell populations and gene expression that may contribute to the development and progression of aortic inflammatory disease. Key findings included enhanced lipid transport capabilities in subpopulations of endothelial cells and upregulation of proinflammatory genes in muscle cells under high-fat diet conditions. Increased numbers of resident and blood-derived macrophages were also observed, potentially promoting excessive immune responses in obese individuals.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Dermatology
Andrew W. Stevenson, Phillip E. Melton, Eric K. Moses, Hilary J. Wallace, Fiona M. Wood, Suzanne Rea, Patricia L. Danielsen, Mansour Alghamdi, Nicole Hortin, Julia Borowczyk, Zhenjun Deng, Mitali Manzur, Mark W. Fear
Summary: In this study, the researchers identified FOXF2 as an important regulator of scar maintenance. They found that FOXF2 is involved in maintaining normotrophic scars by regulating the expression of extracellular matrix-related genes. This study provides insights into the maintenance of scars and suggests that targeting genes responsible for scar phenotype may improve scar appearance and patient outcomes in the future.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Multidisciplinary Sciences
Huy Q. Dinh, Feng Pan, Geng Wang, Qing-Feng Huang, Claire E. Olingy, Zhi-Yong Wu, Shao-Hong Wang, Xin Xu, Xiu-E Xu, Jian-Zhong He, Qian Yang, Sandra Orsulic, Marcela Haro, Li-Yan Li, Guo-Wei Huang, Joshua J. Breunig, H. Phillip Koeffler, Catherine C. Hedrick, Li-Yan Xu, De-Chen Lin, En-Min Li
Summary: This study utilized single-cell RNA sequencing to characterize the heterogeneous microenvironment of ESCC, identifying a tumor-specific subset of CST1(+) myofibroblasts with prognostic values and immunosuppressive features. The analysis of stromal cell types in the ESCC microenvironment provides a rich resource for further understanding of ESCC biology.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Florian Ingelfinger, Lisa Ann Gerdes, Vladyslav Kavaka, Sinduya Krishnarajah, Ekaterina Friebel, Edoardo Galli, Pascale Zwicky, Reinhard Furrer, Christian Peukert, Charles-Antoine Dutertre, Klara Magdalena Eglseer, Florent Ginhoux, Andrea Flierl-Hecht, Tania Kumpfel, Donatella De Feo, Bettina Schreiner, Sarah Mundt, Martin Kerschensteiner, Reinhard Hohlfeld, Eduardo Beltran, Burkhard Becher
Summary: This study investigated the influence of genetic predisposition and environmental factors on the peripheral immune signatures in monozygotic twins discordant for multiple sclerosis (MS). Through data-driven computational tools and high-throughput single-cell technologies, the researchers identified an inflammatory shift in a specific group of immune cells in twins with MS, as well as the emergence of certain hyper-responsive immune cells. They found that the variance in CD25 expression by certain helper T cells with a naive phenotype was largely driven by genetic and shared early environmental influences. However, the expansion of helper T cells in twins with MS, which were also elevated in non-twin patients with MS, appeared to be independent of individual genetic makeup and correlated with disease severity.
Article
Fisheries
Jiayu Chen, Jinji Lin, Feifei Yu, Zhiming Zhong, Qiwen Liang, Huanying Pang, Siyu Wu
Summary: This study analyzed the immune strategy of Crassostrea hongkongensis (C. hongkongensis) against Vibrio parahaemolyticus (V. Parahemolyticus) infection through immune enzyme activities and transcriptome analysis. The findings revealed significant increases in immune enzyme activities and differential gene expression after infection, suggesting the stimulation of immune response in C. hongkongensis by V. parahaemolyticus. The enrichment of immune-related genes in Toll-like receptor (TLR) pathway was identified through transcriptome analysis, and the upregulation of TLR4-MyD88 pathway genes was validated using qRT-PCR. These results indicate the involvement of TLR4-MyD88 pathway in the immune response of C. hongkongensis against Vibrio infection.
AQUACULTURE REPORTS
(2022)
Article
Cell Biology
Qiuxia Zhou, Changlong Zhao, Zhiyong Yang, Rui Qu, Yunbo Li, Yining Fan, Jinlin Tang, Ting Xie, Zilong Wen
Summary: We used single-cell RNA sequencing to comprehensively profile the heterogeneity of tissue-resident macrophages (TRMs) and dendritic cells (DCs) in adult zebrafish organs. We identified two subtypes of macrophages with different functions in barrier tissues, liver, and heart, as well as conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) in these organs. In the brain, we also found a unique population of macrophages/microglia with potent phagocytosis capacity, a population of pDCs, and two distinct populations of cDCs. This study provides valuable insights into the landscape of TRMs and DCs in zebrafish and offers tools for further research.
Article
Cell Biology
Swathy O. Vasudevan, Ashley J. Russo, Puja Kumari, Sivapriya Kailasan Vanaja, Vijay A. Rathinam
Summary: CD14 plays a vital role in intracellular LPS sensing by mediating the cytosolic localization of LPS, leading to the activation of caspase-11. This study provides evidence for the critical role of CD14 in noncanonical inflammasome sensing of LPS.
Article
Biochemistry & Molecular Biology
Yi Liu, Qing Chen, Jian-Zheng Yang, Xiu-Wen Li, Li-Jian Chen, Kai-Kai Zhang, Jia-Li Liu, Jia-Hao Li, Clare Hsu, Long Chen, Jia-Hao Zeng, Qi Wang, Dong Zhao, Jing-Tao Xu, Bernard Lebleu, Celestino Sardu
Summary: In this study, a mouse model of Takotsubo syndrome (TTS) was established to investigate the role of the sigma-1 receptor (Sigmar1) in TTS development. It was found that Sigmar1 knockout exacerbated cardiac dysfunction and led to dysregulation of the gut microbiome, tryptophan metabolism, and several signaling pathways, which play important roles in TTS pathogenesis. These findings provide valuable insights into the function of Sigmar1 in TTS and suggest that it may be a promising therapeutic target.
Article
Multidisciplinary Sciences
Zhuoning Zou, Chuanxin Zhang, Qiuyan Wang, Zhenzhen Hou, Zhuqing Xiong, Feng Kong, Qiujun Wang, Jinzhu Song, Boyang Liu, Bofeng Liu, Lijuan Wang, Fangnong Lai, Qiang Fan, Wenrong Tao, Shuai Zhao, Xiaonan Ma, Miao Li, Keliang Wu, Han Zhao, Zi-Jiang Chen, Wei Xie
Summary: This study utilized Ribo-lite and mRNA sequencing to analyze the transcriptome and translatome of human oocytes and early embryos. The results showed widespread differential translation during the oocyte-to-embryo transition (OET) and zygotic genome activation (ZGA), functioning in epigenetic reprogramming, transposon defense, and small RNA biogenesis. Additionally, TPRX1/2/L were identified as critical regulators of ZGA.
Article
Multidisciplinary Sciences
Marco Orecchioni, Kouji Kobiyama, Holger Winkels, Yanal Ghosheh, Sara McArdle, Zbigniew Mikulski, William B. Kiosses, Zhichao Fan, Lai Wen, Yunmin Jung, Payel Roy, Amal J. Ali, Yukiko Miyamoto, Matthew Mangan, Jeffrey Makings, Zhihao Wang, Angela Denn, Jenifer Vallejo, Michaela Owens, Christopher P. Durant, Simon Braumann, Navid Mader, Lin Li, Hiroaki Matsunami, Lars Eckmann, Eicke Latz, Zeneng Wang, Stanley L. Hazen, Klaus Ley
Summary: This study found that mouse vascular macrophages express the olfactory receptor Olfr2, which can detect and activate the inflammasome, leading to interleukin-1b secretion. It was also discovered that human and mouse blood plasma contains octanal, which can activate Olfr2 and its human ortholog OR6A2. Increasing octanal levels exacerbates atherosclerosis, while targeting Olfr2 gene significantly reduces atherosclerotic plaques. These findings suggest that inhibiting OR6A2 may be a promising strategy for preventing and treating atherosclerosis.
Article
Cell Biology
Rebecca Elena Mainz, Stefanie Albers, Madhuri Haque, Roland Sonntag, Nicole Simone Treichel, Thomas Clavel, Eicke Latz, Kai Markus Schneider, Christian Trautwein, Tobias Otto
Summary: This study evaluates a novel mouse model for alcoholic liver disease (ALD) and investigates the role of NLRP6 inflammasome in intestinal homeostasis and ALD progression. The study shows that alcohol consumption triggers liver injury and changes in intestinal microbial communities. Inhibiting NLRP6 signaling has a minor effect on intestinal homeostasis but reduces hepatic immune cell infiltration. These findings suggest a disease-aggravating role of NLRP6 in ALD.
Article
Cell Biology
Lena F. Schimke, Alexandre H. C. Marques, Gabriela Crispim Baiocchi, Caroline Aliane de Souza Prado, Dennyson Leandro M. Fonseca, Paula Paccielli Freire, Desiree Rodrigues Placa, Igor Salerno Filgueiras, Ranieri Coelho Salgado, Gabriel Jansen-Marques, Antonio Edson Rocha Oliveira, Jean Pierre Schatzmann Peron, Gustavo Cabral-Miranda, Jose Alexandre Marzagao Barbuto, Niels Olsen Saraiva Camara, Vera Lucia Garcia Calich, Hans D. Ochs, Antonio Condino-Neto, Katherine A. Overmyer, Joshua J. Coon, Joseph Balnis, Ariel Jaitovich, Jonas Schulte-Schrepping, Thomas Ulas, Joachim L. Schultze, Helder I. Nakaya, Igor Jurisica, Otavio Cabral-Marques
Summary: Severe COVID-19 patients show similarities with other hyperinflammatory conditions, such as HLH, in terms of clinical and laboratory features. This study highlights the shared immunological pathways and neutrophil-mediated immune responses between COVID-19 and HLH. Dysregulated neutrophil-associated genes and proteins are observed in COVID-19 patients admitted to the ICU, suggesting a generalized hyperinflammatory state. These findings provide potential therapeutic targets for intervention.
Article
Otorhinolaryngology
Marleen Heiming, Eleftherios Savvas, Claudia Rudack, Markus Stenner, Christoph Spiekermann
Summary: This study analyzed the impact of the discrepancy between patients' subjective perception and external evaluation of nasal appearance on postoperative satisfaction in functional rhinoplasty (fRPL). The results showed that patients with a large discrepancy between self- and external estimation had a higher perception of the influence of nasal appearance on quality of life. This finding helps identify patients with unrealistic expectations regarding surgical outcomes to avoid postoperative dissatisfaction.
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
(2023)
Article
Immunology
Kevin Bassler, Wataru Fujii, Theodore S. Kapellos, Erika Dudkin, Nico Reusch, Ari Horne, Benedikt Reiz, Malte D. Luecken, Collins Osei-Sarpong, Stefanie Warnat-Herresthal, Lorenzo Bonaguro, Jonas Schulte-Schrepping, Allon Wagner, Patrick Guenther, Carmen Pizarro, Tina Schreiber, Rainer Knoll, Lisa Holsten, Charlotte Kroeger, Elena De Domenico, Matthias Becker, Kristian Haendler, Christian T. Wohnhaas, Florian Baumgartner, Meike Koehler, Heidi Theis, Michael Kraut, Marc H. Wadsworth, Travis K. Hughes, Humberto J. Ferreira, Emily Hinkley, Ines H. Kaltheuner, Matthias Geyer, Christoph Thiele, Alex K. Shalek, Andreas Feisst, Daniel Thomas, Henning Dickten, Marc Beyer, Patrick Baum, Nir Yosef, Anna C. Aschenbrenner, Thomas Ulas, Jan Hasenauer, Fabian J. Theis, Dirk Skowasch, Joachim L. Schultze
Summary: Despite the high prevalence of COPD, the cellular and molecular mechanisms of the disease remain poorly understood. This study investigates the changes in cellular and molecular compositions in the alveolar space and peripheral blood of COPD patients compared to controls. The findings suggest that myeloid cells, including invading monocytes and proliferating macrophages, are increased in COPD. Computational modeling predicts TGF-beta 1 as a major regulator of transcriptional changes in alveolar macrophages of COPD patients. Functionally, macrophages in COPD exhibit impaired immune activation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Endocrinology & Metabolism
Joshua Jackson, Lena Wischhof, Enzo Scifo, Anna Pellizzer, Yiru Wang, Antonia Piazzesi, Debora Gentile, Sana Siddig, Miriam Stork, Chris E. Hopkins, Kristian Handler, Joachim Weis, Andreas Roos, Joachim L. Schultze, Pierluigi Nicotera, Dan Ehninger, Daniele Bano
Summary: Mitochondrial retrograde signaling can stimulate organelle biogenesis as a compensatory adaptation to abnormal activity of the oxidative phosphorylation (OXPHOS) system. In this study, the researchers investigated the molecular mechanisms promoting mitochondrial maintenance in energy-deprived cells and identified the sphingosine phosphate lyase SPL-1/SGPL1 and the ATFS-1-target HOPS complex subunit VPS-39/VPS39 as critical lifespan modulators. They also found that VPS39 recruitment to the mitochondria may be a common signature associated with altered OXPHOS system in mouse-derived muscles.
MOLECULAR METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Lucia Cottone, Lorena Ligammari, Hang-Mao Lee, Helen J. Knowles, Stephen Henderson, Sara Bianco, Christopher Davies, Sandra Strauss, Fernanda Amary, Ana Paula Leite, Roberto Tirabosco, Kristian Haendler, Joachim L. Schultze, Javier Herrero, Paul O'Donnell, Agamemnon E. Grigoriadis, Paolo Salomoni, Adrienne M. Flanagan
Summary: Oncohistones provide compelling evidence for the role of epigenetic perturbations in cancer. Giant cell tumors of bone (GCTs) are characterized by a mutated histone H3.3 as the sole genetic driver in bone-forming osteoprogenitor cells. We show that the H3.3-G34W mutation leads to changes in the transcriptome and epigenome of mesenchymal cells, resulting in increased recruitment of osteoclasts. This mechanism helps explain the response of GCT to denosumab, a drug that depletes osteoclasts in the tumor.
CELL DEATH AND DIFFERENTIATION
(2022)
Correction
Immunology
Natalie Katzmarski, Jorge Dominguez-Andres, Branko Cirovic, Georgios Renieris, Eleonora Ciarlo, Didier Le Roy, Konstantin Lepikhov, Kathrin Kattler, Gilles Gasparoni, Kristian Haendler, Heidi Theis, Marc Beyer, Jos W. M. van der Meer, Leo A. B. Joosten, Joern Walter, Joachim L. Schultze, Thierry Roger, Evangelos J. Giamarellos-Bourboulis, Andreas Schlitzer, Mihai G. Netea
Article
Biology
Niels J. M. Verstegen, Sabrina Pollastro, Peter-Paul A. Unger, Casper Marsman, George Elias, Tineke Jorritsma, Marij Streutker, Kevin Bassler, Kristian Haendler, Theo Rispens, Joachim L. Schultze, Anja ten Brinke, Marc Beyer, S. Marieke van Ham
Summary: In this study, the differentiation trajectories of human naive B cells into antibody-secreting cells (ASCs) were investigated using single-cell RNA sequencing. By comparing transcriptomes of B cells at different stages of differentiation from in vitro and ex vivo sources, a novel pre-ASC population was identified in lymphoid tissues. This study also revealed the existence of a germinal-center-like population in vitro, which may undergo an alternative route of differentiation to become memory B cells, mimicking in vivo human germinal center reactions. This work provides a detailed characterization of human B cell differentiation into ASCs or memory B cells under healthy and diseased conditions.
Review
Immunology
Sophie Mueller, Charlotte Kroeger, Joachim L. Schultze, Anna C. Aschenbrenner
Summary: Blood, as a non-invasive way to access the human immune system, is an important tool for studying physiological and pathophysiological processes. Stimulating whole blood ex vivo allows for investigation of immune responses and has applications in clinical research such as host immunity, disease characterization, and drug development.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Meeting Abstract
Immunology
T. Pecht, J. Loesch, L. Holsten, N. Reusch, A. M. Schieren, S. Koch, L. Bonaguro, J. Schulte-Schrepping, M. van Uelft, E. De-Domenico, R. Dolscheid-Pommerich, M. Schmolz, A. C. Aschenbrenner, T. Ulas, M. -C. Simon, J. L. Schultze
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Cell Biology
Amit Frishberg, Emma Kooistra, Melanie Nuesch-Germano, Tal Pecht, Neta Milman, Nico Reusch, Stefanie Warnat-Herresthal, Niklas Bruse, Kristian Haendler, Heidi Theis, Michael Kraut, Esther van Rijssen, Bram van Cranenbroek, Hans JPM. Koenen, Hidde Heesakkers, Mark van den Boogaard, Marieke Zegers, Peter Pickkers, Matthias Becker, Anna C. Aschenbrenner, Thomas Ulas, Fabian J. Theis, Shai S. Shen-Orr, Joachim L. Schultze, Matthijs Kox
Summary: This study applies a computational scheme to model the recovery dynamics of severe COVID-19 and reveals the cellular and molecular mechanisms underlying the recovery process. The decrease in mature neutrophils during recovery is found to have a direct impact on disease outcome, and global regulatory changes in gene programs are observed, decoupled from cell compositional changes. The findings present a clinically relevant computational framework for modeling disease recovery and provide insights for future studies on the recovery dynamics in other diseases and tissues.
CELL REPORTS MEDICINE
(2022)
Article
Immunology
Joachim L. Schultze, Maren Buettner, Matthias Becker
Summary: This article discusses the potential benefits of artificial intelligence and blockchain technologies in human immunology. It focuses on how Swarm Learning could facilitate collaborative worldwide immunology studies by sharing insights instead of data, while fully respecting local data privacy regulations.
NATURE REVIEWS IMMUNOLOGY
(2022)
Meeting Abstract
Endocrinology & Metabolism
Lucia Cottone, Lorena Ligammari, Helen J. Knowles, Hang-Mao Lee, Stephen Henderson, Sara Bianco, Christopher Davies, Fernanda Amary, Ana Paula Leite, Roberto Tirabosco, Paul O'Donnell, Kristian Haendler, Joachim L. Schultze, Javier Herrero, Agamemnon E. Grigoriadis, Paolo Salomoni, Adrienne M. Flanagan
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
R. Bellmas Sanz, A. Hitz, E. Chichelnitskiy, B. Wiegmann, K. Blaesing, W. Sommer, F. Ius, J. F. Kuehne, A. Knoefel, L. Horn, I. Tudorache, A. Haverich, D. Jonigk, M. Greer, T. S. Kapellos, K. Bassler, J. L. Schultze, G. Warnecke, C. S. Falk
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2022)
