Article
Biology
George Elias, Pieter Meysman, Esther Bartholomeus, Nicolas De Neuter, Nina Keersmaekers, Arvid Suls, Hilde Jansens, Aisha Souquette, Hans De Reu, Marie-Paule Emonds, Evelien Smits, Eva Lion, Paul G. Thomas, Geert Mortier, Pierre Van Damme, Philippe Beutels, Kris Laukens, Viggo Van Tendeloo, Benson Ogunjimi
Summary: This study demonstrates the significant impact of preexisting memory CD4 T cell clones on vaccine-induced immunity to hepatitis B. The presence of these clones can predict the strength and timing of the immune response to vaccination.
Article
Immunology
Jinchan Li, Simon Reinke, Yu Shen, Lena Schollmeyer, Yuk-Chien Liu, Zixu Wang, Sebastian Hardt, Christian Hipfl, Ute Hoffmann, Stefan Frischbutter, Hyun-Dong Chang, Tobias Alexander, Carsten Perka, Helena Radbruch, Zhihai Qin, Andreas Radbruch, Jun Dong
Summary: Circulating and bone marrow resident memory T cells reactive to SARS-CoV-2 have been found in individuals without prior exposure to the virus or vaccine. These memory T cells may play a crucial role in shaping the immune response to SARS-CoV-2 and contribute to the establishment of long-lasting immunity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Kai M. T. Sauerwein, Christoph B. Geier, Roman F. Stemberger, Hueseyin Akyaman, Peter Illes, Michael B. Fischer, Martha M. Eibl, Jolan E. Walter, Hermann M. Wolf
Summary: Previous studies have shown inconclusive results about the ability of patients with common variable immunodeficiency (CVID) to produce vaccine-specific IgG antibodies following COVID-19 vaccination. This study aimed to investigate the immune responses in patients with CVID and milder forms of primary antibody deficiency after receiving the BNT162b2 vaccine. The results demonstrated that normal IgG responses were associated with intact vaccine-specific activation of CXCR5-negative CD4(+) memory T cells in patients with CVID and other milder forms of antibody deficiency. However, defective activation of circulating T follicular helper cells was observed. Impaired TCR-mediated activation of CXCR5-negative CD4(+) memory T cells following vaccination identified patients with primary antibody deficiency and impaired IgG responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Laura Esparcia-Pinedo, Pedro Martinez-Fleta, Noelia Ropero, Paula Vera-Tome, Hugh T. Reyburn, Jose M. Casasnovas, Jose M. Rodriguez Frade, Mar Vales-Gomez, Carlos Vilches, Enrique Martin-Gayo, Cecilia Munoz-Calleja, Francisco Sanchez-Madrid, Arantzazu Alfranca
Summary: The immune response to the SARS-CoV-2 vaccination is important for developing new vaccines and optimizing prevention strategies. Analysis of the adaptive immunity in healthy donors and convalescent individuals before and after vaccination revealed specific changes in CD4+ T cell reactivity and antibody levels.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Carolyn Rydyznski Moderbacher, Christina Kim, Jose Mateus, Joyce Plested, Mingzhu Zhu, Shane Cloney-Clark, Daniela Weiskopf, Alessandro Sette, Louis Fries, Gregory Glenn, Shane Crotty
Summary: NVX-CoV2373 vaccine induces CD4(+) and CD8+ T cell responses, and the production of CD4(+) T cells is correlated with the generation of neutralizing antibodies. This suggests that robust CD4(+) T cell generation may be a key characteristic of NVX-CoV2373 in supporting humoral immune responses.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Immunology
Astrid Hendriks, Malgorzata Ewa Mnich, Bruna Clemente, Ana Rita Cruz, Simona Tavarini, Fabio Bagnoli, Elisabetta Soldaini
Summary: The skin, as an immunocompetent tissue, contains various immune cells and a diverse microbiome. CD4(+) T cells play a crucial role in protecting against Staphylococcus aureus infection, and skin-specific CD4(+) T cells have been identified in response to S. aureus.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Katharina Wild, Maike Smits, Saskia Killmer, Shirin Strohmeier, Christoph Neumann-Haefelin, Bertram Bengsch, Florian Krammer, Martin Schwemmle, Maike Hofmann, Robert Thimme, Katharina Zoldan, Tobias Boettler
Summary: The effectiveness of seasonal influenza vaccines varies between individuals and can be influenced by factors such as vaccination history. Specific CD4 T cell and antibody responses are closely linked to pre-existing immunity and vaccine history. Pre-vaccination immune phenotype and vaccination history play crucial roles in determining influenza hemagglutinin-specific T and B cell responses.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Chin-Yu Wang, Bai-Ling Lin, Chung-Hsuan Chen
Summary: The DNA aptamer Ap52, developed against the MAGE-A3(111-125) peptide antigen, can target multiple types of cancer cells and achieve targeted delivery when complexed with the anticancer drug doxorubicin (Dox). Image analysis shows selective entry of the ThioAp52-Dox complex into cancer cells, highlighting its potential as a delivery vehicle for therapeutics against multiple cancers.
CANCER BIOLOGY & THERAPY
(2021)
Article
Immunology
Ian Shannon, Chantelle L. White, Hongmei Yang, Jennifer L. Nayak
Summary: This study compared influenza-specific CD4 T-cell responses in children who were either acutely infected with influenza or vaccinated with IIV. The results showed that infection primed a stronger CD4 T-cell response specific for the infecting HA and NP proteins, with more robust NP-specific immunity persisting through year 2.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Oncology
Aaron D. Martin, Xueyin Wang, Mark L. Sandberg, Kathleen R. Negri, Ming L. Wu, Dora Toledo Warshaviak, Grant B. Gabrelow, Michele E. McElvain, Bella Lee, Mark E. Daris, Han Xu, Alexander Kamb
Summary: The study suggests that the previously hypothesized MAGE-A12 may not be the primary source of neurotoxicity, and an alternative related peptide, EPS8L2, may be more likely responsible. These findings provide evidence for reconsidering MAGE-A3 as a cancer target.
JOURNAL OF IMMUNOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Jeffrey M. Jacobson, Julie K. Jadlowsky, Simon F. Lacey, Joseph A. Fraietta, Gabriela Plesa, Hideto Chono, Dong H. Lee, Irina Kulikovskaya, Chelsie Bartoszek, Fang Chen, Lifeng Tian, Alexander Dimitri, Bruce L. Levine, Elizabeth A. Veloso, Wei-Ting Hwang, Carl H. June
Summary: The study of a single infusion of MazF-modified CD4 T lymphocytes in HIV-infected individuals demonstrated potential therapeutic effects, including increased CD4 and CD8 cell counts, sustained MazF expression and activity, and detection of modified cells in blood and lymph tissue. Further research on the impact on viremia and latent proviral reservoir is needed.
Review
Immunology
Dawit Wolday, Francis M. Ndungu, Gloria P. Gomez-Perez, Tobias F. Rinke de Wit
Summary: Chronic immune activation is observed in Africans and may be influenced by environmental factors like parasitic infections. It could be related to the immune hypo-responsiveness to infections and vaccines previously observed in this population.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Narmadha Subramanian, Kaisa Hofwimmer, Beatriz Tavira, Lucas Massier, Daniel P. Andersson, Peter Arner, Jurga Laurencikiene
Summary: This study aimed to investigate the association between the inflammatory status of white adipose tissue (WAT) and cardiometabolic diseases in non-obese individuals. The results showed that in individuals with type 2 diabetes and cardiovascular disease, the levels of CCL18 and CD4(+) T cells in WAT were significantly elevated. These findings suggest a potential role of WAT inflammation in the risk of cardiometabolic diseases.
CARDIOVASCULAR DIABETOLOGY
(2023)
Article
Immunology
Qiao Liu, Lisha Wang, Huayu Lin, Zhiming Wang, Jialin Wu, Junyi Guo, Shuqiong Wen, Ling Ran, Zhengliang Yue, Xingxing Su, Qing Wu, Jianfang Tang, Zhirong Li, Li Hu, Lifan Xu, Lilin Ye, Qizhao Huang
Summary: Cytotoxic CD8(+) T cells are the main focus of anti-tumor immunity and immunotherapy research, but transferred tumor-specific CD8(+) T cells have limited effect on suppressing tumor growth. On the other hand, CD4(+) T cells play an important role in curtailing tumor metastasis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Georges Abboud, Seung-Chu Choi, Xiaojuan Zhang, Yuk Pheel Park, Nathalie Kanda, Leilani Zeumer-Spataro, Morgan Terrell, Xiangyu Teng, Kirsten Nundel, Mark J. Shlomchik, Laurenc Morel
Summary: The activation of lymphocytes in lupus is associated with increased cellular metabolism, particularly glycolysis. Inhibiting glycolysis reversed the expansion of specific T and B cells and reduced autoantibody production in lupus-prone mice. However, foreign antigens were not affected, suggesting a unique sensitivity of autoreactive B and CD4+ T cells to glycolysis.
JOURNAL OF IMMUNOLOGY
(2023)
Article
Oncology
Helen X. Chen, Minkyung Song, Holden T. Maecker, Sacha Gnjatic, David Patton, J. Jack Lee, Stacey J. Adam, Radim Moravec, Xiaole Shirley Liu, Ethan Cerami, James Lindsay, Ming Tang, F. Stephen Hodi, Catherine J. Wu, Ignacio I. Wistuba, Gheath Al-Atrash, Chantale Bernatchez, Sean C. Bendall, Stephen M. Hewitt, Elad Sharon, Howard Streicher, Rebecca A. Enos, Melissa D. Bowman, Valerie M. Tatard-Leitman, Beatriz Sanchez-Espiridion, Srinika Ranasinghe, Mina Pichavant, Diane M. Del Valle, Joyce Yu, Sylvie Janssens, Jenny Peterson-Klaus, Cathy Rowe, Gerold Bongers, Robert R. Jenq, Chia-Chi Chang, Jeffrey S. Abrams, Margaret Mooney, James H. Doroshow, Lyndsay N. Harris, Magdalena Thurin
Summary: Immunoprofiling for identifying biomarkers and integrating with clinical trial outcomes play a critical role in improving immunotherapy for cancer patients. The CIMAC-CIDC Network aims to establish an infrastructure for systematic biomarker identification and correlation with clinical outcomes across trials, facilitated by harmonized assays and computational expertise. By establishing this network, immuno-oncology biomarker development and cross-trial data analysis can be enhanced.
CLINICAL CANCER RESEARCH
(2021)
Article
Biochemical Research Methods
Daniel Geanon, Brian Lee, Edgar Gonzalez-Kozlova, Geoffrey Kelly, Diana Handler, Bhaskar Upadhyaya, John Leech, Ronaldo M. De Real, Manon Herbinet, Assaf Magen, Diane Del Valle, Alexander Charney, Seunghee Kim-Schulze, Sacha Gnjatic, Miriam Merad, Adeeb H. Rahman
Summary: Mass cytometry is a powerful tool for immune phenotyping, but its wide deployment is hindered by complex workflows and specialized equipment requirements. The streamlined workflow presented in this study, using commercially available reagents, addresses these challenges and enables broader adoption of CyTOF immune monitoring. The workflow allows for easy staining of whole blood samples on site, followed by batch processing at a central facility, showcasing disease-related changes in immune cells.
Editorial Material
Gastroenterology & Hepatology
Serre-Yu Wong, Rebekah Dixon, Vicky Martinez Pazos, Sacha Gnjatic, Jean-Frederic Colombel, Ken Cadwell
Article
Pathology
Clare Bryce, Zachary Grimes, Elisabet Pujadas, Sadhna Ahuja, Mary Beth Beasley, Randy Albrecht, Tahyna Hernandez, Aryeh Stock, Zhen Zhao, Mohamed Rizwan AlRasheed, Joyce Chen, Li Li, Diane Wang, Adriana Corben, G. Kenneth Haines, William H. Westra, Melissa Umphlett, Ronald E. Gordon, Jason Reidy, Bruce Petersen, Fadi Salem, Maria Isabel Fiel, Siraj M. El Jamal, Nadejda M. Tsankova, Jane Houldsworth, Zarmeen Mussa, Brandon Veremis, Emilia Sordillo, Melissa R. Gitman, Michael Nowak, Rachel Brody, Noam Harpaz, Miriam Merad, Sacha Gnjatic, Wen-Chun Liu, Michael Schotsaert, Lisa Miorin, Teresa A. Aydillo Gomez, Irene Ramos-Lopez, Adolfo Garcia-Sastre, Ryan Donnelly, Patricia Seigler, Calvin Keys, Jennifer Cameron, Isaiah Moultrie, Kae-Lynn Washington, Jacquelyn Treatman, Robert Sebra, Jeffrey Jhang, Adolfo Firpo, John Lednicky, Alberto Paniz-Mondolfi, Carlos Cordon-Cardo, Mary E. Fowkes
Summary: The study reports the first 100 COVID-19-positive autopsies performed in New York City, revealing widespread effects including diffuse alveolar damage, large pulmonary emboli, microthrombi in multiple organ systems, and presence of the virus in samples. Laboratory results showed elevated inflammatory markers, abnormal coagulation values, and increased cytokines.
Review
Oncology
Lorenzo Iovino, Laurel A. Thur, Sacha Gnjatic, Aude Chapuis, Filippo Milano, Joshua A. Hill
Summary: COVID-19 and IMTX-related toxicities share potential common mechanisms and differences, with anti-inflammatory therapy potentially reducing mortality in COVID-19 by targeting cytokines involved in its pathogenesis, particularly by inhibiting the interleukin-6 pathway. Other approaches based on novel drugs and cell therapies are currently under investigation and may lead to a reduction in hospitalization and mortality due to COVID-19.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Correction
Oncology
Arta M. Monjazeb, Anita Giobbie-Hurder, Ana Lako, Emily M. Thrash, Ryan C. Brennick, Katrina Z. Kao, Claire Manuszak, Ryan D. Gentzler, Anteneh Tesfaye, Salma K. Jabbour, Olatunji B. Alese, Osama E. Rahma, James M. Cleary, Elad Sharon, Harvey J. Mamon, May Cho, Howard Streicher, Helen X. Chen, Mansoor M. Ahmed, Adrian Marino-Enriquez, Seunghee Kim-Schulze, Sacha Gnjatic, Emanual Maverakis, Alina I. Marusina, Alexander A. Merleev, Mariano Severgnini, Kathleen L. Pfaff, James Lindsay, Jason L. Weirather, Srinika Ranasinghe, Alexander Spektor, Scott J. Rodig, F. Stephen Hodi, Jonathan D. Schoenfeld
CLINICAL CANCER RESEARCH
(2021)
Article
Oncology
Guray Akturk, Edwin R. Parra, Evisa Gjini, Ana Lako, J. Jack Lee, Donna Neuberg, Jiexin Zhang, Shen Yao, Ilaria Laface, Anita Rogic, Pei-Hsuan Chen, Beatriz Sanchez-Espiridion, Diane M. Del Valle, Radim Moravec, Robert Kinders, Courtney Hudgens, Catherine Wu, Ignacio I. Wistuba, Magdalena Thurin, Stephen M. Hewitt, Scott Rodig, Sacha Gnjatic, Michael T. Tetzlaff
Summary: This study compared and harmonized protocols, platforms, and technologies across multiple laboratories, confirming the interchangeability of protocols and platforms between different sites to obtain robust and comparable data. This also indicates that CIMAC-CIDC analyses can be confidently used for statistical associations with clinical outcomes.
CLINICAL CANCER RESEARCH
(2021)
Article
Oncology
Bita Sahaf, Mina Pichavant, Brian H. Lee, Caroline Duault, Emily M. Thrash, Melanie Davila, Nicolas Fernandez, Karen Millerchip, Salah-Eddine Bentebibel, Cara Haymaker, Natalia Sigal, Diane M. Del Valle, Srinika Ranasinghe, Sarah Fayle, Beatriz Sanchez-Espiridion, Jiexin Zhang, Chantale Bernatchez, Catherine J. Wu, Ignacio I. Wistuba, Seunghee Kim-Schulze, Sacha Gnjatic, Sean C. Bendall, Minkyung Song, Magdalena Thurin, J. Jack Lee, Holden T. Maecker, Adeeb Rahman
Summary: The study aimed to identify biomarkers of response to cancer immunotherapies across clinical trials using state-of-the-art assays and conducted cross-site data harmonization efforts. Results show the ability to reproduce CyTOF data across sites in multicenter trials and emphasize the importance of quality control procedures.
CLINICAL CANCER RESEARCH
(2021)
Article
Multidisciplinary Sciences
Noam D. Beckmann, Phillip H. Comella, Esther Cheng, Lauren Lepow, Aviva G. Beckmann, Scott R. Tyler, Konstantinos Mouskas, Nicole W. Simons, Gabriel E. Hoffman, Nancy J. Francoeur, Diane Marie Del Valle, Gurpawan Kang, Anh Do, Emily Moya, Lillian Wilkins, Jessica Le Berichel, Christie Chang, Robert Marvin, Sharlene Calorossi, Alona Lansky, Laura Walker, Nancy Yi, Alex Yu, Jonathan Chung, Matthew Hartnett, Melody Eaton, Sandra Hatem, Hajra Jamal, Alara Akyatan, Alexandra Tabachnikova, Lora E. Liharska, Liam Cotter, Brian Fennessy, Akhil Vaid, Guillermo Barturen, Hardik Shah, Ying-chih Wang, Shwetha Hara Sridhar, Juan Soto, Swaroop Bose, Kent Madrid, Ethan Ellis, Elyze Merzier, Konstantinos Vlachos, Nataly Fishman, Manying Tin, Melissa Smith, Hui Xie, Manishkumar Patel, Kai Nie, Kimberly Argueta, Jocelyn Harris, Neha Karekar, Craig Batchelor, Jose Lacunza, Mahlet Yishak, Kevin Tuballes, Ieisha Scott, Arvind Kumar, Suraj Jaladanki, Charuta Agashe, Ryan Thompson, Evan Clark, Bojan Losic, Lauren Peters, Panagiotis Roussos, Jun Zhu, Wenhui Wang, Andrew Kasarskis, Benjamin S. Glicksberg, Girish Nadkarni, Dusan Bogunovic, Cordelia Elaiho, Sandeep Gangadharan, George Ofori-Amanfo, Kasey Alesso-Carra, Kenan Onel, Karen M. Wilson, Carmen Argmann, Supinda Bunyavanich, Marta E. Alarcon-Riquelme, Thomas U. Marron, Adeeb Rahman, Seunghee Kim-Schulze, Sacha Gnjatic, Bruce D. Gelb, Miriam Merad, Robert Sebra, Eric E. Schadt, Alexander W. Charney
Summary: Multisystem inflammatory syndrome in children (MIS-C) presents symptoms similar to Kawasaki disease and affects individuals under 21 years of age. RNA sequencing revealed signatures related to natural killer cell activation and CD8+ T cell exhaustion in the blood of MIS-C patients, showing similarities with Kawasaki disease.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Sapna P. Patel, Gina R. Petroni, Jason Roszik, Walter C. Olson, Nolan A. Wages, Kimberly A. Chianese-Bullock, Mark Smolkin, Nikole Varhegyi, Elizabeth Gaughan, Kelly T. Smith, Kathleen Haden, Emily H. Hall, Sacha Gnjatic, Patrick Hwu, Craig L. Slingluff
Summary: This study evaluated the safety and immunogenicity of a novel long peptide vaccine with different adjuvants, showing that the LPV7 vaccine induced certain immune responses under various adjuvant strategies, but did not significantly enhance T cell responses compared to vaccines using short peptides.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Oncology
Mary Zhang, Julie A. Hong, Tricia F. Kunst, Colleen D. Bond, Cara M. Kenney, Cheryl L. Warga, Javier Yeray, Min-Jung Lee, Akira Yuno, Sunmin Lee, Markku Miettinen, R. Taylor Ripley, Chuong D. Hoang, Sacha Gnjatic, Jane B. Trepel, David S. Schrump
Summary: This study demonstrates that H1299 lysate vaccines with Iscomatrix™ can induce immune responses to CTA and decrease the percentage of Treg cells while modulating the expression of immune-related molecules on other cells. However, metronomic cyclophosphamide and celecoxib did not enhance the vaccine-induced immune responses.
TRANSLATIONAL LUNG CANCER RESEARCH
(2021)
Article
Oncology
Junko Matsuzaki, Shashikant Lele, Kunle Odunsi, Takemasa Tsuji
Summary: Adoptive cell therapy (ACT) is a promising immunotherapy that provides cancer antigen-specific effector T cells for cancer patients. This study characterized the immunogenicity of CLDN6 in ovarian cancer patients and identified specific TCR genes from CD8(+) and CD4(+) T cells, which are important for the treatment of ovarian and other solid tumors expressing CLDN6.
Article
Oncology
Myvizhi Esai Selvan, Kenan Onel, Sacha Gnjatic, Robert J. Klein, Zeynep H. Gumus
Summary: Recent studies have shown that rare, deleterious variants (RDVs) in specific genes play a critical role in heritable cancer risk. By analyzing whole-exome sequencing data of 20,789 participants, we identified associations between cancer risk and RDVs in DNA repair, cancer predisposition, and somatic cancer drivers. Moreover, we found that personal RDV load in these gene-sets is associated with increased risk, earlier onset, increased M1 macrophages in tumor, and increased tumor mutational burden in specific cancers. These findings can be used to identify high-risk individuals and improve prognosis through increased surveillance, earlier screening, and targeted treatments.
NPJ PRECISION ONCOLOGY
(2023)
Article
Oncology
Beryl L. Manning-Geist, Sacha Gnjatic, Carol Aghajanian, Jason Konner, Sarah H. Kim, Debra Sarasohn, Krysten Soldan, William P. Tew, Nicholas J. Sarlis, Dmitriy Zamarin, Sara Kravetz, Ilaria Laface, Teresa Rasalan-Ho, Jingjing Qi, Phillip Wong, Paul J. Sabbatini, Roisin E. O'Cearbhaill
Summary: A combination therapy of WT1 peptide vaccine and nivolumab has been found to induce immune responses in ovarian cancer patients, and it is safe and tolerable. This study provides additional evidence for the combination of immune checkpoint inhibitors and vaccines.
Meeting Abstract
Oncology
Camille Bigenwald, Jessica Le Berichel, Rikhia Chakraborty, Steven T. Chen, Guray Akturk, Alexandra Tabachnikova, Anahita Rafiei, Ilaria Laface, Howard Lim, Alessia Baccarini, Poulikos I. Poulikakos, Brian D. Brown, Sacha Gnjatic, Amaia Lujambio, Markus G. Man, Kenneth L. McClain, Jennifer Picarsic, Carl E. Allen, Miriam Merad
PEDIATRIC BLOOD & CANCER
(2021)