Review
Biochemistry & Molecular Biology
Adriana Aurelia Chis, Carmen Maximiliana Dobrea, Luca-Liviu Rus, Adina Frum, Claudiu Morgovan, Anca Butuca, Maria Totan, Anca Maria Juncan, Felicia Gabriela Gligor, Anca Maria Arseniu
Summary: Gene-directed enzyme prodrug therapy (GDEPT) is a promising strategy for prodrug delivery, with benefits including enhanced efficacy and reduced off-target toxicity. Non-viral vectors, such as dendrimers, have gained interest due to their decreased immunogenicity and high specificity for delivering genes in GDEPT therapy.
Article
Chemistry, Multidisciplinary
Yi Yan, Xiao-Yu Liu, An Lu, Xiang-Yu Wang, Lin-Xia Jiang, Jian-Cheng Wang
Summary: RNA-based therapy is a promising strategy for disease treatment, and recent advances in non-viral delivery systems have shown potential in protecting RNA, facilitating cell internalization, and controlled release of therapeutics.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Materials Science, Multidisciplinary
Jing Chen, Wen Nie, Yuzhu Hu, Yangmei Shen, Yunzhu Lin, Bilan Wang, Zhiyong Qian, Xiang Gao
Summary: Drug resistance is a challenging problem in cancer chemotherapy. The combination of molecular targeted therapy and traditional chemotherapy shows potential clinical benefits. A folic acid-modified targeted drug/gene delivery system was developed to co-deliver Paclitaxel (PTX) and plasmid shBCL-2, which can enhance the sensitivity of cancer cells to chemotherapy drugs and improve therapeutic effect.
APPLIED MATERIALS TODAY
(2022)
Article
Multidisciplinary Sciences
Agata Antepowicz, Omar Habib, Freja Kirsebom, Cecilia Johansson, Deborah R. Gill, Stephen C. Hyde
Summary: In this study, gene delivery approaches using recombinant adeno-associated virus and simian immunodeficiency virus vectors were utilized to achieve sustained in vivo production of Palivizumab in a murine model. Pre-treatment with Palivizumab-expressing vectors provided complete protection against RSV-induced weight loss. This approach offers prophylaxis against RSV infection, potentially reducing treatment costs in vulnerable populations.
SCIENTIFIC REPORTS
(2021)
Review
Pharmacology & Pharmacy
Hui Zu, Danchen Gao
Summary: Although non-viral vectors in gene therapy have low cytotoxicity and immunogenicity, they still face challenges in gene transfer efficiency, specificity, gene expression duration, and safety. Overcoming these obstacles is necessary to enhance the effectiveness of non-viral vectors in gene therapy.
Review
Engineering, Biomedical
Jiayu Gu, Zhourui Xu, Qiqi Liu, Shiqi Tang, Wenguang Zhang, Shouxia Xie, Xiaoyan Chen, Jiajie Chen, Ken-Tye Yong, Chengbin Yang, Gaixia Xu
Summary: In recent years, messenger RNA (mRNA) vaccines have shown great potential as replacements for conventional vaccines due to their low risk of mutagenesis, safety and effectiveness, rapid and scalable production, and low-cost manufacturing. Non-viral mRNA vectors are considered ideal choices for future clinical translation due to their safety, sufficient packaging capability, low immunogenicity, and versatility. This review focuses on the recent progress in the development of non-viral mRNA vectors, including lipid nanoparticles, polymers, peptides, and exosomes. It also discusses the latest advances in clinical trials of mRNA vaccines, as well as the current challenges and future possibilities for the clinical translation of these vectors.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Organic
Michela Lomazzi, Valentina Franceschi, Valentina Bagnacani, Carlo Alberto Vezzoni, Gaetano Donofrio, Alessandro Casnati, Francesco Sansone
Summary: The tetra-L-arginino-tetrahexyloxycalix[4]arene 1 has shown remarkable abilities to compact and deliver various types of nucleic acid cargos into cells. Small structural changes in the calixarene vectors may affect their ability to compact DNA and deliver cargo into cells.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Review
Biotechnology & Applied Microbiology
Ilona Uzieliene, Ursule Kalvaityte, Eiva Bernotiene, Ali Mobasheri
Summary: Gene therapy has been explored as a potential treatment for osteoarthritis, with a focus on non-viral gene delivery technologies. While viral gene delivery has been more popular due to higher efficiency, efforts to enhance transfection efficiency of non-viral methods have shown promise for OA research and clinical applications. More research is needed to optimize transfection efficiency and duration of gene expression.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Chenfei Wang, Chaolan Pan, Haiyang Yong, Feifei Wang, Tao Bo, Yitong Zhao, Bin Ma, Wei He, Ming Li
Summary: Gene therapy holds great promise for treating a wide range of genetic diseases by delivering functional genes into targeted cells or tissues. However, the lack of safe and efficient gene delivery vehicles remains a major obstacle to its clinical implementation. This review comprehensively outlines the novel non-viral gene delivery vectors with potential applications in gene therapy.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Yu Wang, Yan Tang, Xiao-mei Zhao, Gui Huang, Jin-hong Gong, Shu-di Yang, Hui Li, Wen-jun Wan, Chang-hao Jia, Gang Chen, Xue-nong Zhang
Summary: In this study, a multifunctional non-viral vector was constructed for efficient delivery of CRISPR/Cas9 plasmid. The vector actively targeted tumor cells and delivered the cargo into cell nuclei, resulting in genome editing effects. By disrupting the MTH1 gene, the growth of non-small cell lung cancer was inhibited, promoting cell apoptosis in tumor tissue and reducing liver metastasis.
ACTA BIOMATERIALIA
(2022)
Review
Chemistry, Multidisciplinary
Shuai Qu, Renfa Liu, Nisi Zhang, Yunxue Xu, Xiuli Yue, Zhifei Dai
Summary: This article reviews the recent advances in targeted non-viral nucleic acid delivery for gene therapy of atherosclerosis, including hepatocyte-targeted delivery and delivery to different cells within the plaques.
Article
Chemistry, Multidisciplinary
Keisuke Fukunaga, V. Dhamodharan, Nao Miyahira, Yoko Nomura, Kamila Mustafina, Yasuaki Oosumi, Kosuke Takayama, Akira Kanai, Yohei Yokobayashi
Summary: Synthetic riboswitches that can regulate gene expression by small molecules in mammalian cells have potential applications in biotechnology and medicine. However, the variety of small molecules and their aptamers for mammalian cells is limited. We developed RNA aptamers against a small molecule ASP7967 and used them to design riboswitches for gene regulation. The riboswitches showed high activation potential even at low molecule concentrations. They were also successfully used in regulating gene expression in vivo and achieving a high ON/OFF ratio in cultured cells.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Biochemistry & Molecular Biology
Darya A. Savenkova, Aelita-Luiza A. Makarova, Igor K. Shalik, Dmitry V. Yudkin
Summary: Gene therapy is commonly used for treating incurable disorders, often using viral vectors to deliver genetic material. However, viral vectors have disadvantages such as immune response and disruption of cell metabolism, which require evaluation of side effects and safety. Many gene therapy techniques use non-coding RNA to regulate gene expression, but this can affect cellular miRNA pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Shan He, Ju Fang, Chuanxin Zhong, Fuzeng Ren, Min Wang
Summary: The study developed a non-viral DNA delivery nanocomplex that was immobilized onto a polydopamine-coated electrospun alginate nanofibrous scaffold, creating a gene-activated matrix (GAM) for enhanced skin wound healing. The nanocomplex showed low cytotoxicity and high transfection efficiency. The GAM enabled sustained gene release and long-term transgene expression, accelerating wound closure, promoting re-epithelization, reducing inflammatory response, and enhancing neovascularization in a rat model.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Multidisciplinary
Siying Li, Xuelei Pang, Jing Zhao, Qingrong Zhang, Yuping Shan
Summary: Targeted peptides are important in the construction of intelligent drug delivery systems, and the interaction between peptides and receptors is crucial for the efficacy of targeted nano-drugs. Using single-molecule force spectroscopy, this study evaluated the stability and effectiveness of three common peptides targeting specific receptors on living cell surfaces. The T7-TfR bond was found to be the most stable among the three peptide-receptor pairs.
Article
Anesthesiology
Hailin Zhao, Han Huang, Rele Ologunde, Dafydd G. Lloyd, Helena Watts, Marcela P. Vizcaychipi, Qingquan Lian, Andrew J. T. George, Daqing Ma
Article
Engineering, Biomedical
Pei Yun Teo, Chuan Yang, Lynsey M. Whilding, Ana C. Parente-Pereira, John Maher, Andrew J. T. George, James L. Hedrick, Yi Yan Yang, Sadaf Ghaem-Maghami
ADVANCED HEALTHCARE MATERIALS
(2015)
Article
Oncology
Jayanta Chatterjee, Wei Dai, Nor Haslinda Abd Aziz, Pei Yun Teo, John Wahba, David L. Phelps, Christian J. Maine, Lynsey M. Whilding, Roberto Dina, Giorgia Trevisan, Kirsty J. Flower, Andrew J. T. George, Sadaf Ghaem-Maghami
CLINICAL CANCER RESEARCH
(2017)
Review
Medicine, General & Internal
Hailin Zhao, Azeem Alam, Aurelie Pac Soo, Andrew J. T. George, Daqing Ma
Article
Engineering, Biomedical
Pei Yun Teo, Chuan Yang, James L. Hedrick, Amanda C. Engler, Daniel J. Coady, Sadaf Ghaem-Maghami, Andrew J. T. George, Yi Yan Yang
Article
Oncology
Christian J. Maine, Nor Haslinda Abdul Aziz, Jayanta Chatterjee, Claudia Hayford, Nancy Brewig, Lynsey Whilding, Andrew J. T. George, Sadaf Ghaem-Maghami
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2014)
Article
Immunology
Adnan Khan, Hongmei Fu, Lee Aun Tan, Jennifer E. Harper, Sven C. Beutelspacher, Daniel F. P. Larkin, Giovanna Lombardi, Myra O. McClure, Andrew J. T. George
EUROPEAN JOURNAL OF IMMUNOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Hailin Zhao, Jiaolin Ning, Sinead Savage, Heechan Kang, Kaizhi Lu, Xia Zheng, Andrew J. T. George, Daqing Ma
Article
Biochemistry & Molecular Biology
Hailin Zhao, Akira Yoshida, Wei Xiao, Rele Ologunde, Kieran P. O'Dea, Masao Takata, Catherine Tralau-Stewart, Andrew J. T. George, Daqing Ma
Article
Urology & Nephrology
Hailin Zhao, Xianghong Luo, Zhaowei Zhou, Juying Liu, Catherine Tralau-Stewart, Andrew J. T. George, Daqing Ma
KIDNEY INTERNATIONAL
(2014)
Letter
Medicine, General & Internal
Catherine E. Urch, Andrew J. T. George
BMJ-BRITISH MEDICAL JOURNAL
(2020)
Article
Cardiac & Cardiovascular Systems
Andrew J. T. George
Summary: This article discusses the ethical issues related to pig to human cardiac xenotransplantation, including risks/benefits, animal welfare and rights, fair allocation and resource utilization concerns. The author proposes an alternative approach based on virtue ethics, exploring the virtues of justice, courage, temperance, practical wisdom, and clinical curiosity to reflect on clinical practices in this field.
Article
Psychology, Applied
Andrew J. T. George
Summary: Complexity theory is a powerful tool for understanding complex interactions in group development. However, it is underutilized in the coaching literature. This paper discusses the implications of non-linear dynamics on group development and provides a toy model of anger in a group of three as an illustration. Although formal mathematical modeling may not be feasible, the insights can help coaches intervene effectively and bring maximum benefits to their clients.
INTERNATIONAL JOURNAL OF EVIDENCE BASED COACHING & MENTORING
(2022)
Review
Physiology
Hailin Zhao, Jessica Santivanez Perez, Kaizhi Lu, Andrew J. T. George, Daqing Ma
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2014)
Article
Immunology
Petros X. E. Mouratidis, Andrew J. T. George
JOURNAL OF INFLAMMATION RESEARCH
(2015)