Article
Veterinary Sciences
Putri Pandarangga, Milton M. McAllister, Anne E. Peaston, Yuen T. Ngai, Mohammad I. Cahyono, Farhid Hemmatzadeh
Summary: Antigenic differences between commercial Newcastle Disease Virus (NDV) vaccine and circulating field virus reduce vaccine efficacy. Superior results are obtained when serological testing, vaccinations, and vaccine challenge experiments all use circulating strains of ND virus.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Analytical
Yanke Shan, Bin Wang, Huachuan Huang, Keding Yan, Wenzhi Li, Shouyu Wang, Fei Liu
Summary: In this study, a high-throughput multimodal immunoassay platform is proposed for rapid on-site diagnostics of COVID-19. The platform utilizes fluorescent, colorimetric, and chemiluminescent immunoassays, and can simultaneously detect multiple samples, providing high sensitivity and fast detection.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Tohid Mahmoudi, Mohammad Pourhassan-Moghaddam, Behnaz Shirdel, Behzad Baradaran, Eden Morales-Narvaez, Hamed Golmohammadi
Summary: A convenient kit was developed for on-site detection of CEA in human serum, utilizing cellulose-based lateral flow strips and a smartphone-based system for signal reading and processing. Excellent correlation was demonstrated in clinical sample testing, showing potential for monitoring cancer treatment.
Article
Immunology
Christelle Kassis, Michelle Durkin, Eric Holbrook, Robert Myers, Lawrence Wheat
Summary: Combining antibody and antigen detection can improve sensitivity and specificity in the diagnosis of coccidioidomycosis, particularly with higher sensitivity in immunocompromised patients. Antigen detection can help prevent missed diagnoses.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Chemistry, Analytical
Xianxian Zhao, Ye Yuan, Xiaoya Liu, Fajiang Mao, Ge Xu, Qingzhong Liu
Summary: The development of a versatile and sensitive analytical biomarker detection platform is crucial for early disease diagnosis and treatment. In this study, a novel fluorescence-based, ultrasensitive, and label-free biomarker detection platform is proposed. This platform demonstrates high sensitivity and specificity, and shows great potential in clinical diagnosis and anticancer drug development.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Xiao-Kun Zhang, Hong-Ming Yang, Meng-Ran Li, Xiao-Yi Gao, Xiao-Wei Sun, Xi-Feng Sun, Jin-Bao Tang
Summary: The study introduces a new method for orienting antibodies on the surface of liposomes, enhancing the detection efficiency of novel immunoliposomes with Fc region-specific and covalent conjugation for prostate-specific antigen. The immunoliposomes showed a significantly improved detection sensitivity, making them potential for sensitive detection of various biomarkers in complex serum samples.
Article
Microbiology
Dane Granger, Nicholas T. Streck, Elitza S. Theel
Summary: This study suggests that a single antigen detection assay can be used to simultaneously detect Histoplasma and Blastomyces, with a high level of diagnostic accuracy. By utilizing the cross-reactivity between the two antigen detection assays, test utilization can be optimized and patient costs can be decreased.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Jeonghyo Kim, Van Tan Tran, Sangjin Oh, Minji Jang, Dong Kun Lee, Jong Chul Hong, Tae Jung Park, Hwa-Jung Kim, Jaebeom Lee
Summary: The MagPlas NZs, combining magnetic enrichment and catalytic amplification, offer a streamlined, high-throughput operation with ultrahigh sensitivity in urine-based tuberculosis diagnosis and anti-TB therapy monitoring. The MagPlas ELISA method showed fast (<3 h) and highly sensitive (up to pM) detection of urinary TB antigen, with robust sensitivity and high specificity in clinical studies. This methodology promises noninvasive therapeutic response monitoring and has potential for use in point-of-care testing for TB control and other acute infections.
ACS CENTRAL SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Jin Wang, Xing Shen, Peng Zhong, Zhaodong Li, Qiushi Tang, Xinan Huang, Anatoly V. Zherdev, Boris B. Dzantiev, Sergei A. Eremin, Zhili Xiao, Hongtao Lei, Xiangmei Li
Summary: A heterologous immunoassay strategy was designed to enhance the sensitivity for the detection of banned dye RB in fraudulent food in this study. The proposed immunoassay can be used for the surveillance screening of RB in a range of seasoning foods, indicating the heterologous strategy is an effective approach to enhance sensitivity in an immunoassay.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2021)
Article
Chemistry, Analytical
Li Hou, Juanjuan Huang, Shendong Liu, Tianran Lin, Shulin Zhao
Summary: The study utilized three-dimensional liposome networks as signal amplification modules to linearly assay carcinoembryonic antigen (CEA) using a magneto-controlled fluorescent immunosensor, with a detection range from 0.05 ng mL-1 to 40 ng mL-1 and a detection limit of 13.2 pg mL-1 (3 sigma/K).
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Public, Environmental & Occupational Health
Jaeeun Yoo, Younjeong Kim, Yu mi Cha, Jaewoong Lee, Yeon Jeong Jeong, Si-Hyun Kim, Lisa L. Maragakis, Seungok Lee
Summary: This study compared the efficacy of three different immunization methods against the wild-type, delta, and omicron variants of SARS-CoV-2. The results showed that heterologous booster vaccination (AZ/AZ/BNT) provided the highest level of protection against the omicron variant, followed closely by COVID-19 patients.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Article
Biotechnology & Applied Microbiology
Chenchit Chayachinda, Kanokwaroon Watananirun, Chayawat Phatihattakorn, Sanitra Anuwutnavin, Suvimol Niyomnaitham, Wanatpreeya Phongsamart, Keswadee Lapphra, Orasri Wittawatmongkol, Supattra Rungmaitree, Laddawan Jansarikit, Kobporn Boonnak, Patimaporn Wongprompitak, Sansnee Senawong, Avishek Upadhya, Zheng Quan Toh, Paul V. V. Licciardi, Kulkanya Chokephaibulkit
Summary: This trial evaluated the immunogenicity and reactogenicity of different COVID-19 vaccination schedules in pregnant Thai women. Heterologous schedules of CoronaVac-BNT162b2 or ChAdOx1-BNT162b2 showed similar immunogenicity to BNT162b2-BNT162b2. Neutralizing antibody levels were highest in the CoronaVac-BNT162b2 group. No significant difference in cord blood antibody levels between groups were observed. Local and systemic adverse events were mild-to-moderate and more frequent in the ChAdOx1-BNT162b2 group.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2023)
Article
Biotechnology & Applied Microbiology
Renata Novakova, Dagmar Homerova, Dominika Csolleiova, Bronislava Rezuchova, Beatrica Sevcikova, Rachel Javorova, Lubomira Feckova, Jan Kormanec
Summary: Bacteria of the genus Streptomyces are important producers of biologically active natural products. However, many of these products are silent under laboratory conditions. In this study, we optimized a strain of Streptomyces lavendulae for heterologous gene expression and successfully integrated reporter genes and biosynthetic gene clusters into a vector system. This study provides valuable insights into harnessing the biosynthetic potential of Streptomyces bacteria.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Yanke Shan, Yufan Feng, Jiahao Li, Weijie Yi, Meng Ge, Huachuan Huang, Keding Yan, Shouyu Wang, Fei Liu
Summary: A portable fluorescence resonance energy transfer (FRET)-based enzyme-linked immunosorbent assay (ELISA) was designed for on-site detection of the porcine epidemic diarrhea virus (PEDV). The FRET immunoassay achieved high sensitivity and accuracy, and could detect PEDV in a 96-well plate in just 35 minutes.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Qiong Wu, Guannan Chen, Sufang Qiu, Shangyuan Feng, Duo Lin
Summary: The study developed a target-triggered and self-calibration aptasensor based on SERS technology for sensitive and reliable detection of PSA protein, with a promising potential for accurate screening of prostate cancer in human blood samples. The method showed comparable detection accuracy, a larger dynamic detection range, a lower detection limit, and lower sample requirement compared to the clinical commonly used method.
Article
Biochemistry & Molecular Biology
Meenakshi Tiwari, Anima Tripathi, Shail K. Chaube
Article
Biochemistry & Molecular Biology
Anumegha Gupta, Meenakshi Tiwari, Shilpa Prasad, Shail K. Chaube
JOURNAL OF CELLULAR BIOCHEMISTRY
(2017)
Review
Cell Biology
Meenakshi Tiwari, Shilpa Prasad, Tulsidas G. Shrivastav, Shail K. Chaube
JOURNAL OF CELLULAR PHYSIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Meenakshi Tiwari, Anumegha Gupta, Alka Sharma, Shilpa Prasad, Ashutosh N. Pandey, Pramod K. Yadav, Ajai K. Pandey, Tulsidas G. Shrivastav, Shail K. Chaube
JOURNAL OF CELLULAR BIOCHEMISTRY
(2018)
Review
Medicine, Research & Experimental
Ajai K. Pandey, Anumegha Gupta, Meenakshi Tiwari, Shilpa Prasad, Ashutosh N. Pandey, Pramod K. Yadav, Alka Sharma, Kankshi Sahu, Syed Asrafuzzaman, Doyil T. Vengayil, Tulsidas G. Shrivastav, Shail K. Chaube
BIOMEDICINE & PHARMACOTHERAPY
(2018)
Article
Cell Biology
Kankshi Sahu, Anumegha Gupta, Alka Sharma, Meenakshi Tiwari, Ashutosh N. Pandey, Shilpa Prasad, Pramod K. Yadav, Ajai K. Pandey, Tulsidas G. Shrivastav, Shail K. Chaube
Article
Cell Biology
Alka Sharma, Meenakshi Tiwari, Anumegha Gupta, Ashutosh N. Pandey, Pramod K. Yadav, Shail K. Chaube
JOURNAL OF CELLULAR PHYSIOLOGY
(2018)
Review
Cell Biology
Govind R. Chaudhary, Pramod K. Yadav, Anil K. Yadav, Meenakshi Tiwari, Anumegha Gupta, Alka Sharma, Kankshi Sahu, Ashutosh N. Pandey, Ajai K. Pandey, Shail K. Chaube
JOURNAL OF CELLULAR PHYSIOLOGY
(2019)
Review
Biochemistry & Molecular Biology
Anil Kumar Yadav, Pramod K. Yadav, Govind R. Chaudhary, Meenakshi Tiwari, Anumegha Gupta, Alka Sharma, Ashutosh N. Pandey, Ajai K. Pandey, Shail K. Chaube
CELLULAR AND MOLECULAR LIFE SCIENCES
(2019)
Article
Pharmacology & Pharmacy
Anumegha Gupta, Shail K. Chaube
EUROPEAN JOURNAL OF PHARMACOLOGY
(2020)
Article
Pharmacology & Pharmacy
Anumegha Gupta, Ashutosh N. Pandey, Alka Sharma, Meenakshi Tiwari, Pramod K. Yadav, Anil K. Yadav, Ajai K. Pandey, Tulsidas G. Shrivastav, Shail K. Chaube
EUROPEAN JOURNAL OF PHARMACOLOGY
(2020)
Article
Cell & Tissue Engineering
Karuppanan Premkumar, Shilpa Prasad, Meenakshi Tiwari, Ashutosh N. Pandey, Anumegha Gupta, Alka Sharma, Pramod K. Yadav, Anil K. Yadav, Devendra K. Pandey, Ajai K. Pandey, Shail K. Chaube
Summary: Meiotic instability leads to abortive spontaneous ovum activation (SOA), affecting egg quality and fertilization outcomes. Signal molecules such as ROS, cAMP, and Ca2+ are involved in the induction of meiotic instability. Global attention is needed to identify and address meiotic instability in ova, with potential use of activated ova in regenerative medicine.
STEM CELL REVIEWS AND REPORTS
(2021)
Review
Cell Biology
Govind R. Chaudhary, Pramod K. Yadav, Anil K. Yadav, Meenakshi Tiwari, Anumegha Gupta, Alka Sharma, Ashutosh N. Pandey, Ajai K. Pandey, Shail K. Chaube
JOURNAL OF BIOMEDICAL SCIENCE
(2019)
Review
Cell Biology
Pramod K. Yadav, Meenakshi Tiwari, Anumegha Gupta, Alka Sharma, Shilpa Prasad, Ashutosh N. Pandey, Shail K. Chaube
JOURNAL OF BIOMEDICAL SCIENCE
(2018)
Article
Medicine, Research & Experimental
Meenakshi Tiwari, Shail K. Chaube
BIOMEDICINE & PHARMACOTHERAPY
(2017)