Article
Spectroscopy
Wenlu Wu, Zhongfei Hu, Cai Shi, Ruoqian Xu, Yiming Zhao, Yujie Ding
Summary: A Fe3+ optical sensor was developed by linking CdTe quantum dots and a Rhodamine B derivative using gamma-cyclodextrin as a bridge. The nanoprobe displayed a response to Fe3+ through a fluorescence resonance energy transfer process. The probe showed satisfactory linearity in the fluorescent quenching between 10 and 60 μM Fe3+ concentration, with a limit of detection of 2.51 μM. The probe was successfully used for the determination of Fe3+ in human serum with high sensitivity and exceptional selectivity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Nanoscience & Nanotechnology
Danni Chen, Heng Li, Bin Yu, Junle Qu
Summary: This study proposes a method for researching dynamic events in living cells by simultaneously monitoring spatial positions and changes in local environment related to fluorescence lifetime. The feasibility of the method is verified through experiments, and intracellular endocytosis in a living cell is successfully observed.
Article
Physics, Multidisciplinary
Hong-Yu Tu, Ji-Chao Cheng, Gen-Cai Pan, Lu Han, Bin Duan, Hai-Yu Wang, Qi-Dai Chen, Shu-Ping Xu, Zhen-Wen Dai, Ling-Yun Pan
Summary: Exciton-plasmon composites have become a research hot-spot due to controllable energy transfer on the nanoscale. This study investigated the FRET process in both electrostatic attraction and electrostatic repulsion systems, revealing local-field induced FRET with different lifetime components. The results emphasize the importance of considering both steady and transient state information to elucidate the intrinsic transfer process.
Article
Chemistry, Physical
Okhil K. Nag, Megan E. Muroski, Lauren D. Field, Michael H. Stewart, Eunkeu Oh, Kimihiro Susumu, Joseph R. Spangler, Scott A. Walper, James B. Delehanty
Summary: The study introduces a novel approach utilizing nanoparticle bioconjugates to activate channelrhodopsin C1V1 (ChR-C1V1) in HEK 293T/17 cell membranes. It demonstrates the specific activation of ChR-C1V1 channels through laser-induced opening with photoexcited quantum dots as an energy donor. This work represents a new hybrid system for controlling ion channel activity by interfacing quantum dots with living cells.
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
(2021)
Article
Spectroscopy
Anmol Arora, Kriti Sharma, S. K. Tripathi
Summary: This study investigated the impact of luminescent MoSe2 quantum dots on trypsin activity and found that MoSe2 quantum dots formed a stable complex with trypsin through electrostatic interactions. This finding is significant for the development of trypsin detection methods and understanding the bioavailability of MoSe2 quantum dots in biological systems.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Plant Sciences
Nina Gloeckner, Sven zur Oven-Krockhaus, Leander Rohr, Frank Wackenhut, Moritz Burmeister, Friederike Wanke, Eleonore Holzwart, Alfred J. Meixner, Sebastian Wolf, Klaus Harter
Summary: Protein-protein interaction studies are crucial for understanding cellular signaling. This study used three-fluorophore FRET and FRET-fluorescence lifetime imaging microscopy techniques to demonstrate the formation of a ternary complex involving Receptor-Like Protein 44, Brassinosteroid Insensitive 1, and Brassinosteroid Insensitive 1 Associated Kinase 1 in living plant cells. The researchers also found that this complex is localized in a distinct plasma membrane nanodomain.
Article
Biochemistry & Molecular Biology
Guan-Yu Zhuo, Ming-Chi Chen, Tzu-Yu Lin, Shih-Ting Lin, Daniel Tzu-Li Chen, Cynthia Wei-Sheng Lee
Summary: We investigated the effects of opioids on the coexpressed mu-opioid (MOP) and nociceptin/orphanin FQ (NOP) receptors in human embryonic kidney (HEK) 293 cells. Our findings suggest that morphine, but not buprenorphine, facilitates the formation of MOP-NOP heterodimers and enhances their localization on lipid rafts. Moreover, morphine stimulates the phosphorylation of Erk1/2 in cells expressing MOP and MOP+NOP receptors, while buprenorphine appears to activate Erk1/2 solely through NOP receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Yang Wang, Jingwen Ai, Xiaolin Nie, Zhuquan Li, Xin Xia, Tanveer Hussain, Qingqing Wang, Qufu Wei
Summary: This study focuses on porphyrin-based metal organic frameworks (MOFs) and their application in photodynamic inactivation. By encapsulating carbon quantum dots (CQDs) into the MOFs, the photodynamic activity is enhanced through fluorescence resonance energy transfer (FRET). The CQDs@PCN-224 material is embedded into bacterial cellulose (BC) nanofibrous membranes using a biosynthetic approach, which prevents material leakage and enhances antibacterial efficiency.
CARBOHYDRATE POLYMERS
(2023)
Review
Chemistry, Multidisciplinary
G. Prabakaran, K. Velmurugan, C. Immanuel David, R. Nandhakumar
Summary: Fluorescence sensing based on Forster Resonance Energy Transfer (FRET) using graphene-based nanomaterials has gained significant interest in various fields. Graphene quantum dots (GQDs), graphene oxide (GO), reduced graphene oxide (rGO), and graphdiyne (GD) can be used as donors/acceptors in FRET-based sensing due to their unique properties. This review discusses the basics of FRET and the role of graphene-based nanomaterials in sensing various analytes, as well as explaining the lower detection ranges. Challenges and future directions for designing nanomaterials with improved sensing performance are also highlighted.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Multidisciplinary
Yoshiaki Okamoto, Seiji Iwasa, Ryugo Tero
Summary: Single particle observation of quantum dots on lipid bilayers formed on graphene oxide was conducted in order to detect the quenching efficiency of lipid and protein molecules in the lipid bilayer.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Md. Maisum Sarwar Jyoti, Md. Rubel Rana, Md. Hasan Ali, Toshinobu Tokumoto
Summary: In this study, graphene quantum dots (GQDs) were used for the binding assay of membrane progesterone receptor alpha (mPRα). GQD-mPRα, a fluorescent GQD-conjugated mPRα, was prepared by coupling the amino groups of mPRα with GQDs. The results demonstrated the formation of a complex of GQD-mPRα and the ligand.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Amanpreet Singh, Gagandeep Singh, Navneet Kaur, Narinder Singh
Summary: In this study, a nano sensor system based on biomass-derived carbon quantum dots (CQDs) and naphthalimide was developed for ratiometric detection of nitric oxide (NO) in pure aqueous media. The sensor demonstrated high selectivity towards NO with a detection limit of 15 nM. Moreover, it was also utilized for the indirect detection of nitrite (NO2 ) in food samples for food safety and monitoring.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Applied
Xinyao Wang, Shiqi Liuye, Xinhuan Ma, Shiqiang Cui, Shouzhi Pu
Summary: A novel photo-controlled solid-state fluorescence switching based on carbon dots (CDs) and diarylethene was designed and constructed. The fluorescence switching not only had excellent modulation efficiency, but also exhibited good photochromic properties. The mechanism of the reversible fluorescence modulation was attributed to the FRET between the CDs (energy donor) and the closed-ring diarylethene (energy acceptor). This work suggests the strategy of constructing solid-state fluorescence switching is feasible, and will provide new ways for the potential application of CDs and diarylethene.
Article
Chemistry, Multidisciplinary
S. Roth, P. T. Trinh, J. Wachtveitl
Summary: The photophysics of an inorganic/organic hybrid system were studied, focusing on increasing the two-photon efficiency of photoresponsive systems. The CdS/ZnS core/shell quantum dots and coumarin derivatives demonstrated a highly efficient Forster resonance energy transfer mechanism, even after two-photon excitation.
Article
Biochemical Research Methods
Chidinma A. Okolo, Ilias Kounatidis, Johannes Groen, Kamal L. Nahas, Stefan Balint, Thomas M. Fish, Mohamed A. Koronfel, Aitziber L. Cortajarena, Ian M. Dobbie, Eva Pereiro, Maria Harkiolaki
Summary: This protocol describes sample preparation strategies for correlative 3D cryo-structured illumination microscopy and cryo-soft X-ray tomography, including a direct comparison and recommendations regarding fiducial selection. By combining different microscopy techniques, nanometer resolution imaging can be achieved under cryogenic conditions. Strategic use of markers is crucial for experimental design.
Article
Biochemical Research Methods
Nattawut Sinsuebphon, Alena Rudkouskaya, Margarida Barroso, Xavier Intes
JOURNAL OF BIOPHOTONICS
(2018)
Article
Chemistry, Multidisciplinary
Alena Rudkouskaya, Nattawut Sinsuebphon, Jamie Ward, Kate Tubbesing, Xavier Intes, Margarida Barroso
JOURNAL OF CONTROLLED RELEASE
(2018)
Article
Biochemical Research Methods
Sez-Jade Chen, Nattawut Sinsuebphon, Alena Rudkouskaya, Margarida Barroso, Xavier Intes, Xavier Michalet
JOURNAL OF BIOPHOTONICS
(2019)
Article
Engineering, Biomedical
David M. Kingsley, Cassandra L. Roberge, Alena Rudkouskaya, Denzel E. Faulkner, Margarida Barroso, Xavier Intes, David T. Corr
ACTA BIOMATERIALIA
(2019)
Article
Biochemistry & Molecular Biology
Brennan D. Gerlach, Kate Tubbesing, Guoning Liao, Alyssa C. Rezey, Ruping Wang, Margarida Barroso, Dale D. Tang
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Brennan D. Gerlach, Michael Marinello, Justin Heinz, Nicholas Rymut, Brian E. Sansbury, Colin O. Riley, Sudeshna Sadhu, Zeinab Hosseini, Yoko Kojima, Dale D. Tang, Nicholas J. Leeper, Matthew Spite, Margarida Barroso, Katey J. Rayner, Gabrielle Fredman
CELL DEATH AND DIFFERENTIATION
(2020)
Article
Multidisciplinary Sciences
Lianjie Miao, Jingjing Li, Jian Li, Yangyang Lu, David Shieh, Joseph E. Mazurkiewicz, Margarida Barroso, John J. Schwarz, Hong-Bo Xin, Harold A. Singer, Peter A. Vincent, Weimin Zhong, Glenn L. Radice, Leo Q. Wan, Zhen-Chuan Fan, Guoying Huang, Mingfu Wu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Multidisciplinary Sciences
Jason T. Smith, Ruoyang Yao, Nattawut Sinsuebphon, Alena Rudkouskaya, Nathan Un, Joseph Mazurkiewicz, Margarida Barroso, Pingkun Yan, Xavier Intes
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Oncology
Kate Tubbesing, Jamie Ward, Raymond Abini-Agbomson, Aditi Malhotra, Alena Rudkouskaya, Janine Warren, John Lamar, Nina Martino, Alejandro P. Adam, Margarida Barroso
MOLECULAR CANCER RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Ting Chean Khoo, Kate Tubbesing, Alena Rudkouskaya, Shilpi Rajoria, Anna Sharikova, Margarida Barroso, Alexander Khmaladze
Article
Cardiac & Cardiovascular Systems
Wei Zhang, Jinjing Zhao, Lin Deng, Nestor Ishimwe, Jessica Pauli, Wen Wu, Shengshuai Shan, Wolfgang Kempf, Margaret D. Ballantyne, David Kim, Qing Lyu, Matthew Bennett, Julie Rodor, Adam W. Turner, Yao Wei Lu, Ping Gao, Mihyun Choi, Ganesh Warthi, Ha Won Kim, Margarida M. Barroso, William B. Bryant, Clint L. Miller, Neal L. Weintraub, Lars Maegdefessel, Joseph M. Miano, Andrew H. Baker, Xiaochun Long
Summary: The study discovered that INKILN is involved in the regulation of vascular smooth muscle cell (VSMC) inflammation and plays a role in the development of atherosclerosis and abdominal aortic aneurysm. INKILN activates the expression of inflammatory genes through interaction with MKL1 in VSMCs. This study provides a novel and physiologically relevant approach for investigating human-specific long noncoding RNAs under vascular disease conditions.
Review
Pharmacology & Pharmacy
Margarida Barroso, Michael G. Monaghan, Raluca Niesner, Ruslan I. Dmitriev
Summary: Organoid models play a crucial role in bioengineering applications, addressing developmental and cancer biology, tissue repair, etc. Their complexity makes them suitable for novel imaging methods like FLIM to study cell metabolism.
ADVANCED DRUG DELIVERY REVIEWS
(2023)
Article
Multidisciplinary Sciences
Ling Wang, Joshua Goldwag, Megan Bouyea, Jonathan Barra, Kailie Matteson, Niva Maharjan, Amina Eladdadi, Mark J. Embrechts, Xavier Intes, Uwe Kruger, Margarida Barroso
Summary: We proposed a new methodology, organelle topology-based cell classification pipeline (OTCCP), that accurately classifies cancer cells based on their organelle spatial topology. OTCCP integrates artificial intelligence and imaging quantification to analyze organelle distribution and inter-organelle topology. Organelle topology allows for precise differentiation between cell lines of different subtypes and aggressiveness.
Article
Biophysics
Jason T. Smith, Nattawut Sinsuebphon, Alena Rudkouskaya, Xavier Michalet, Xavier Intes, Margarida Barroso
Summary: Forster resonance energy transfer (FRET) microscopy is widely used for monitoring molecular interactions and conformational changes. It has been extended to in vivo optical imaging, particularly for quantifying drug-target engagement or release in cancer models. This study compares intensity-based FRET and macroscopic fluorescence lifetime (MFLI) FRET for small animal optical in vivo imaging and demonstrates that MFLI-FRET has significant advantages.
BIOPHYSICAL REPORTS
(2023)
Proceedings Paper
Optics
Alena Rudkouskaya, Nattawut Sinsuebphon, Xavier Intes, Margarida Barroso
MOLECULAR-GUIDED SURGERY: MOLECULES, DEVICES, AND APPLICATIONS III
(2017)
