Article
Oceanography
David L. Meyer, John M. Johnson, John Gill, Christopher Doley
Summary: This study found that both oligohaline Phragmites australis and Spartina alterniflora marshes provide similar functions for marsh nekton and estuarine finfish communities, regardless of geographic region. However, there were pronounced differences in marsh nekton use between Chesapeake Bay and North Carolina regions.
OCEAN & COASTAL MANAGEMENT
(2022)
Article
Marine & Freshwater Biology
Giun-Yi Hung, Po-Yen Chen, Jiun-Lin Horng, Li-Yih Lin
Summary: Environmental contamination by anticancer pharmaceuticals has been widely reported, especially their detrimental effects on zebrafish embryonic development and skin functions. Anticancer drug vincristine (VCR) exposure led to significant teratogenic effects and skin ionocytes reduction in zebrafish embryos. These findings highlight the potential developmental defects and impairment risks associated with acute exposure to VCR.
AQUATIC TOXICOLOGY
(2021)
Article
Biology
Erin M. Leonard, Fiona E. Weaver, Colin A. Nurse
Summary: Lactate is produced as a by-product of anaerobic metabolism in most vertebrate cells. It acts both as a fuel and a signaling molecule, and stimulates ventilation in vertebrates that breathe air or water. Recent evidence suggests that lactate affects chemoreceptors in the carotid body of mammals. This study provides direct evidence that gill NECs serve as lactate sensors.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Fei Ding, Shuangye Zhang, Qian Chen, Hao Feng, Zhilei Ge, Xiaolei Zuo, Chunhai Fan, Qian Li, Qiang Xia
Summary: Programmable nucleic acid nanotechnology allows the customization of synthetic nucleic acid nanodevices with unprecedented precision, showing potential for precise immunoengineering.
Article
Biotechnology & Applied Microbiology
Rachael Putman, Adele S. Ricciardi, Kelly E. W. Carufe, Elias Quijano, Raman Bahal, Peter M. Glazer, W. Mark Saltzman
Summary: Gene editing using nanoparticle delivery system has been demonstrated to be effective in correcting gene mutations during early embryonic development, potentially offering a cure or prevention for genetic diseases.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Environmental Sciences
Elizabeth Allmon, Jennifer Serafin, Shuai Chen, Maria L. Rodgers, Robert Griffitt, Thijs Bosker, Sylvain de Guise, Maria S. Sepulveda
Summary: This study investigates the combined effects of PAHs and abiotic stressors on the cardiac transcriptomes of developing Fundulus grandis larvae. The results show that combined exposures impact signaling pathways associated with cardiovascular function, particularly affecting cardiomyocyte proliferation and leading to inhibited cardiac function. This study provides a comprehensive view of the impacts of PAHs and common environmental stressors on the cardiac system in early life stage estuarine species.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Giorgio Ventimiglia, Massimiliano Pesaturo, Alastair Malcolm, Salvatore Petralia
Summary: A silicon lab-on-chip, integrating PCR and hybridization microarray, has been developed for nucleic acid detection. The lab-on-chip shows remarkable sensitivity, multiplexing analysis, and specificity for the detection of target sequences.
Article
Immunology
Rachel Y. Gao, Christine M. Riley, Evan Toth, Rebecca H. Blair, Megan N. Gerold, Caitlin McCormick, Amber W. Taylor, Tianjing Hu, Kathy L. Rowlen, Erica D. Dawson
Summary: The COVID-19 pandemic has emphasized the potential of mRNA as a platform for vaccines and therapeutics. A multiplexed platform (VaxArray) is demonstrated to efficiently verify identity and determine concentration for monovalent and multivalent mRNA formulations. The assay has a short time to result, does not require PCR-based amplification or mRNA extraction from lipid nanoparticles, and exhibits high construct specificity. The sensitivity for influenza hemagglutinin and neuraminidase mRNA is vaccine-relevant, and the assay shows good accuracy and precision.
Review
Chemistry, Analytical
Siyu Chen, Bo Gong, Cong Zhu, Chunyang Lei, Zhou Nie
Summary: Nucleic acid molecules possess attractive properties for biosensing and bioimaging due to their high designability and structural predictability. CRISPR-Cas systems, with high programmability and flexibility, recognize and cleave nucleic acid targets and have been engineered as multifunctional tools. Integration of nucleic acids with CRISPR-Cas systems has advanced biosensing and bioimaging methodologies, holding great promise in biochemical research and clinical diagnosis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Rachelle Turiello, Leah M. Dignan, Brayton Thompson, Melinda Poulter, Jeff Hickey, Jeff Chapman, James P. Landers
Summary: This article describes an automatable rotationally driven microfluidic platform for efficient extraction of SARS-CoV-2 RNA from multiple sample types. The method enables rapid enrichment and extraction of RNA, allowing reliable detection of SARS-CoV-2 using real-time RT-PCR.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Tina Y. Liu, Gavin J. Knott, Dylan C. J. Smock, John J. Desmarais, Sungmin Son, Abdul Bhuiya, Shrutee Jakhanwal, Noam Prywes, Shreeya Agrawal, Maria Diaz de Leon Derby, Neil A. Switz, Maxim Armstrong, Andrew R. Harris, Emeric J. Charles, Brittney W. Thornton, Parinaz Fozouni, Jeffrey Shu, Stephanie Stephens, G. Renuka Kumar, Chunyu Zhao, Amanda Mok, Anthony T. Iavarone, Arturo M. Escajeda, Roger McIntosh, Shin E. Kim, Eli J. Dugan, Katherine S. Pollard, Ming X. Tan, Melanie Ott, Daniel A. Fletcher, Liana F. Lareau, Patrick D. Hsu, David F. Savage, Jennifer A. Doudna
Summary: The direct, amplification-free detection of RNA through CRISPR nucleases enables rapid and sensitive RNA detection, particularly suitable for point-of-care infection diagnosis.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Siqi Gao, Ranfeng Wu, Qiang Zhang
Summary: This article introduces a controllable DNA cross circuit based on a new control method and realizes a programmable DNA tile self-assembly strategy by integrating a DNA cross circuit and DNA tile self-assembly. The design of this system provides a new idea for generating dynamic and controllable trigger elements and broadens the programmable range of stimuli-responsive self-assembly.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Biology
Hajime Shinoda, Tatsuya Iida, Asami Makino, Mami Yoshimura, Junichiro Ishikawa, Jun Ando, Kazue Murai, Katsumi Sugiyama, Yukiko Muramoto, Masahiro Nakano, Kotaro Kiga, Longzhu Cui, Osamu Nureki, Hiroaki Takeuchi, Takeshi Noda, Hiroshi Nishimasu, Rikiya Watanabe
Summary: opn-SATORI is an automated amplification-free digital RNA detection platform that utilizes CRISPR-Cas13a and microchamber device to complete the detection process from sample mixing to RNA quantification in a short period of time. It is capable of detecting SARS-CoV-2 genomic RNA with a low limit of detection and discriminating between variants with high accuracy.
COMMUNICATIONS BIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Huating Kong, Bo Sun, Feng Yu, Qisheng Wang, Kai Xia, Dawei Jiang
Summary: DNA is a robust material with unique properties, making it suitable for building various nanoscale structures and devices. Structural DNA nanotechnology has found applications in computing, photonics, synthetic biology, biosensing, bioimaging, and therapeutic delivery. The goal of structural DNA nanotechnology is to use DNA molecules to construct three-dimensional crystals as molecular scaffolds for precise alignment and collection of desired molecules. This review showcases different 3D DNA crystals, discussing their design, optimization, applications, and crystallization conditions, as well as the history of nucleic acid crystallography and potential future directions in the era of nanotechnology.
Article
Nanoscience & Nanotechnology
Marta Broto, Michael M. Kaminski, Christopher Adrianus, Nayoung Kim, Robert Greensmith, Schan Dissanayake-Perera, Alexander J. Schubert, Xiao Tan, Hyemin Kim, Anand S. Dighe, James J. Collins, Molly M. Stevens
Summary: This study combines CRISPR-Cas reaction with nanozyme-linked ELISA to achieve quantitative detection of Cas13-mediated RNA detection through catalytic metallic nanoparticles at room temperature (CrisprZyme). The study shows that CrisprZyme is adaptable to lateral flow reading and different Cas enzymes, and can detect various non-coding RNAs. The platform can be used to identify disease patients and monitor cellular differentiation. CrisprZyme can serve as a universally applicable signal catalyst for CRISPR-based diagnostics, expanding the target range of preamplification-free, quantitative detection.
NATURE NANOTECHNOLOGY
(2022)