Accelerated RNA detection using tandem CRISPR nucleases

The Card1 nuclease provides defence during type-III CRISPR immunity

(2021) Jakob T. Rostøl et al.   NATURE

Test sensitivity is secondary to frequency and turnaround time for COVID-19 screening

(2021) Daniel B. Larremore et al.   Science Advances

Structure and Mechanism of a Cyclic Trinucleotide-Activated Bacterial Endonuclease Mediating Bacteriophage Immunity

(2020) Rebecca K. Lau et al.   MOLECULAR CELL

Structure and mechanism of a Type III CRISPR defence DNA nuclease activated by cyclic oligoadenylate

(2020) Stephen A. McMahon et al.   Nature Communications

Predicting infectious SARS-CoV-2 from diagnostic samples

(2020) Jared Bullard et al.   CLINICAL INFECTIOUS DISEASES

Massively multiplexed nucleic acid detection with Cas13

(2020) Cheri M. Ackerman et al.   NATURE

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6

(2020) Carmela Garcia-Doval et al.   Nature Communications

The dynamic interplay of host and viral enzymes in type III CRISPR-mediated cyclic nucleotide signalling

(2020) Januka S Athukoralage et al.   eLife

CRISPR/Cas13a Powered Portable Electrochemiluminescence Chip for Ultrasensitive and Specific MiRNA Detection

(2020) Ting Zhou et al.   Advanced Science

Blueprint for a pop-up SARS-CoV-2 testing lab

(2020)   NATURE BIOTECHNOLOGY

Type III-A CRISPR-associated protein Csm6 degrades cyclic hexa-adenylate activator using both CARF and HEPN domains

(2020) Dalia Smalakyte et al.   NUCLEIC ACIDS RESEARCH

Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets

(2020) Chantal B. F. Vogels et al.   Nature Microbiology

Chemistry of Class 1 CRISPR-Cas effectors: Binding, editing, and regulation

(2020) Tina Y. Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

(2020) Julia Joung et al.   NEW ENGLAND JOURNAL OF MEDICINE

Rethinking Covid-19 Test Sensitivity — A Strategy for Containment

(2020) Michael J. Mina et al.   NEW ENGLAND JOURNAL OF MEDICINE

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

(2020) Parinaz Fozouni et al.   CELL

Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2

(2020) Jon Arizti-Sanz et al.   Nature Communications

A Type III CRISPR Ancillary Ribonuclease Degrades Its Cyclic Oligoadenylate Activator

(2019) Januka S. Athukoralage et al.   JOURNAL OF MOLECULAR BIOLOGY

CRISPR-Cas III-A Csm6 CARF Domain Is a Ring Nuclease Triggering Stepwise cA4 Cleavage with ApA>p Formation Terminating RNase Activity

(2019) Ning Jia et al.   MOLECULAR CELL

Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble

(2019) Tina Y. Liu et al.   Nature Communications

Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas

(2019) Rafael Molina et al.   Nature Communications

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

Ring nucleases deactivate type III CRISPR ribonucleases by degrading cyclic oligoadenylate

(2018) Januka S. Athukoralage et al.   NATURE

CRISPR-Cas guides the future of genetic engineering

(2018) Gavin J. Knott et al.   SCIENCE

RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes

(2017) Alexandra East-Seletsky et al.   MOLECULAR CELL

Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers

(2017) Ole Niewoehner et al.   NATURE

A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems

(2017) Migle Kazlauskiene et al.   SCIENCE

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

(2016) Alexandra East-Seletsky et al.   NATURE

Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6

(2016) Ole Niewoehner et al.   RNA

CIDRE: an illumination-correction method for optical microscopy

(2015) Kevin Smith et al.   NATURE METHODS