Editorial Material
Biology
Altaira D. Dearborn, Ashish Kumar, Joseph Marcotrigiano
Summary: A domain in the ORF1 polyprotein of the hepatitis E virus, previously believed to be a protease, is actually a zinc-binding domain.
Article
Biochemistry & Molecular Biology
Akash Kumar, Preeti Hooda, Anindita Puri, Radhika Khatter, Mohammed S. Al-Dosari, Neha Sinha, Mohammad K. Parvez, Deepak Sehgal
Summary: Hepatitis E Virus (HEV) is a RNA virus that requires modulation of specific enzymes for infection prevention. Methotrexate and compound A have been found to inhibit the activity of HEV helicase, thereby inhibiting HEV RNA replication.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Virology
Reid Warsaba, Nikolay Stoynov, Kyung-Mee Moon, Stephane Flibotte, Leonard Foster, Eric Jan
Summary: Viral protein genome-linked (VPg) plays an essential role in protein-primed replication of plus-stranded RNA viruses. Some viruses have multiple VPgs, but their roles are not well-defined. This study using cricket paralysis virus as a model reveals a relationship between VPg copy number and IRES translation activity, and uncovers a novel viral strategy where VPg copies in dicistrovirus genomes compensate for differences in IRES translation efficiencies to promote infection.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Benjamin E. Nilsson-Payant, Daniel Blanco-Melo, Skyler Uhl, Beatriz Escudero-Perez, Silke Olschewski, Patricia Thibault, Maryline Panis, Maria Rosenthal, Cesar Munoz-Fontela, Benhur Lee, Benjamin R. tenOever
Summary: Negative-sense RNA viruses rely on nucleoprotein NP to regulate transcription and replication of the viral genome, and limiting NP levels results in decreased genome replication and enhanced host antiviral response. Insufficient NP prevents NSVs from processing full-length genomes, leading to the formation of aberrant replication products that trigger immune recognition and a strong host antiviral response. The consequences of limiting NP levels are universal among NSVs, including Ebola virus, Lassa virus, and measles virus.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Pan Gong, Qingtang Shen, Mingzhen Zhang, Rui Qiao, Jing Jiang, Lili Su, Siwen Zhao, Shuai Fu, Yu Ma, Linhao Ge, Yaqin Wang, Rosa Lozano-Duran, Aiming Wang, Fangfang Li, Xueping Zhou
Summary: This study discovers the existence of small open reading frames (ORFs) in the negative-sense RNA of plant and animal positive-sense single-stranded RNA viruses. The small proteins encoded by these rORFs play critical roles in viral infection, revealing a viral proteome larger than previously thought.
Article
Biochemistry & Molecular Biology
Morgan R. R. Herod, Joseph C. C. Ward, Andrew Tuplin, Mark Harris, Nicola J. J. Stonehouse, Christopher J. J. McCormick
Summary: Genome replication of positive strand RNA viruses requires the production of a complementary negative strand RNA, but evidence of structural RNA elements in the negative strand is limited. This study found that different viruses have different capacities for the folding of the negative strand RNA, which may explain the limited observation.
Article
Virology
Susannah Stephenson-Tsoris, John L. Casey
Summary: This study identified the start site for HDV genome RNA synthesis as position 1646 and revealed some differences in the synthesis process. The findings are important for targeting virus replication.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Andreas Laras, Margarita Papatheodoridi, Eleni Panopoulou, George Papatheodoridis, Stephanos J. Hadziyannis, Emilia Hadziyannis
Summary: Serum HBV RNA in HBeAg-negative chronically infected patients primarily consists of pgRNA and a minor fraction of preC mRNA transcripts. Serum pgRNA is associated with disease activity and can serve as a marker for the decision of cessation of therapy.
Article
Biochemistry & Molecular Biology
Ziqi Fu, Monica S. Guo, Weiqiang Zhou, Jie Xiao
Summary: This study examines the role of positive and negative supercoiling in the organization of the bacterial genome. The findings suggest that both types of supercoiling enhance spatial contact, with positive supercoiling playing a larger role. Additionally, the majority of supercoiling sites coincide with highly active transcription units.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biology
Robert LeDesma, Brigitte Heller, Abhishek Biswas, Stephanie Maya, Stefania Gili, John Higgins, Alexander Ploss
Summary: This study reveals that the multifunctional protein ORF1 of Hepatitis E virus (HEV) is crucial for genome replication and is not regulated by proteolytic processing. The essential role of six cysteines in the pPCP domain in viral replication is associated with the coordination of divalent metal ions. Moreover, the pPCP domain can only rescue viral genome replication when expressed in the context of the full-length ORF1 protein.
Review
Virology
Justin M. Su, Maxwell Z. Wilson, Charles E. Samuel, Dzwokai Ma
Summary: LLPS plays a key role in the formation of viral inclusion bodies (IBs) in non-segmented negative-sense (NNS) RNA virus-infected cells, revealing the complexity of viral IB factories. The research has important implications for understanding the viral life cycle and host responses to infection.
Review
Microbiology
Aartjan J. W. te Velthuis, Jonathan M. Grimes, Ervin Fodor
Summary: RNA viruses, including important human and animal pathogens, have genomes consisting of single or multiple RNA segments. Replication and transcription are performed by RNA-dependent RNA polymerases, and recent high-resolution studies have provided insights into the structures of these enzymes and their roles in viral transcription and replication, offering potential targets for antiviral drug design.
NATURE REVIEWS MICROBIOLOGY
(2021)
Article
Microbiology
Harini Sooryanarain, S. Ansar Ahmed, Xiang-Jin Meng
Summary: The study found that progesterone significantly increases HEV replication in human liver cells, but does not modulate HEV-induced interferon response. PGRMC1/2 receptors affect the levels of HEV-induced IFN-lambda 1 expression via the ERK pathway, resulting in reduced HEV replication.
Review
Biochemistry & Molecular Biology
Philippe Roingeard, Sebastien Eymieux, Julien Burlaud-Gaillard, Christophe Hourioux, Romuald Patient, Emmanuelle Blanchard
Summary: Positive single-strand RNA (+ RNA) viruses can remodel host cell membranes to create a replication organelle (RO) that protects their genome replication from innate immunity. Certain viruses, such as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), induce double-membrane vesicles (DMVs) for this purpose. Understanding the mechanisms of DMV formation and their role in the infectious cycle of SARS-CoV-2 may be crucial for developing new antiviral strategies against this pathogen or future coronaviruses.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Virology
Min Fu, Hui Zhang, Mengxue Yin, Zhenhao Han, Qing Bai, Yuhong Peng, Karim Shafik, Lifeng Zhai, Ni Hong, Wenxing Xu, Guoping Wang, Ioly Kotta-Loizou
Summary: A novel +ssRNA mycovirus, CfRV1, isolated from Colletotrichum fructicola in China, is phylogenetically related to Hadaka virus 1 and polymycoviruses, expanding our understanding of virus diversity and evolution.
JOURNAL OF VIROLOGY
(2022)
Article
Pharmacology & Pharmacy
Amit Kumar, Laurie Coquard, Georges Herbein
CURRENT DRUG TARGETS
(2016)
Review
Virology
Wasim Abbas, Muhammad Tariq, Mazhar Iqbal, Amit Kumar, Georges Herbein
Review
Oncology
Amit Kumar, Gilles Darcis, Carine Van Lint, Georges Herbein
CLINICAL EPIGENETICS
(2015)
Review
Oncology
Wasim Abbas, Amit Kumar, Georges Herbein
FRONTIERS IN ONCOLOGY
(2015)
Article
Multidisciplinary Sciences
Amit Kumar, Wasim Abbas, Laurence Colin, Kashif Aziz Khan, Sophie Bouchat, Audrey Varin, Anis Larbi, Jean-Stephane Gatot, Kabamba Kabeya, Caroline Vanhulle, Nadege Delacourt, Sebastien Pasquereau, Laurie Coquard, Alexandra Borch, Renate Koenig, Nathan Clumeck, Stephane De Wit, Olivier Rohr, Christine Rouzioux, Tamas Fulop, Carine Van Lint, Georges Herbein
SCIENTIFIC REPORTS
(2016)
Article
Oncology
Amit Kumar, Laurie Coquard, Sebastien Pasquereau, Laetitia Russo, Severine Valmary-Degano, Christophe Borg, Pierre Pothier, Georges Herbein
MOLECULAR THERAPY-ONCOLYTICS
(2016)
Review
Virology
Sebastien Pasquereau, Amit Kumar, Georges Herbein
Article
Virology
Sebastien Pasquereau, Amit Kumar, Wasim Abbas, Georges Herbein
Article
Medicine, General & Internal
Amit Kumar, Manoj Kumar Tripathy, Sebastien Pasquereau, Fatima Al Moussawi, Wasim Abbas, Laurie Coquard, Kashif Aziz Khan, Laetitia Russo, Marie-Paule Algros, Severine Valmary-Degano, Olivier Adotevi, Stephanie Morot-Bizot, Georges Herbein
Article
Multidisciplinary Sciences
Amit Kumar, Wasim Abbas, Sophie Bouchat, Jean-Stephane Gatot, Sebastien Pasquereau, Kabamba Kabeya, Nathan Clumeck, Stephane De Wit, Carine Van Lint, Georges Herbein
SCIENTIFIC REPORTS
(2016)
Article
Multidisciplinary Sciences
Fatima Al Moussawi, Amit Kumar, Sebastien Pasquereau, Manoj K. Tripathy, Walid Karam, Mona Diab-Assaf, Georges Herbein
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Sebastien Pasquereau, Perle Totoson, Zeina Nehme, Wasim Abbas, Amit Kumar, Frank Verhoeven, Clement Prati, Daniel Wendling, Celine Demougeot, Georges Herbein
Summary: The study showed that SIRT1 expression and activity increased in PBMCs and liver of rats with adjuvant-induced arthritis, which decreased under glucocorticoid treatment. Levels of proinflammatory cytokines IL-1 beta and TNF in the liver decreased under high doses of glucocorticoids. A strong positive correlation was found between liver levels of TNF and IL-1 beta.
Article
Oncology
Sandy Haidar Ahmad, Fatima Al Moussawi, Ranim El Baba, Zeina Nehme, Sebastien Pasquereau, Amit Kumar, Chloe Molimard, Franck Monnien, Marie-Paule Algros, Racha Karaky, Thomas Stamminger, Mona Diab Assaf, Georges Herbein
Summary: Research suggests a close link between HCMV and breast tumor development, with the presence of the UL69 gene in CTH cells and tumor biopsies further highlighting the direct role of HCMV in oncogenesis.
FRONTIERS IN ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Sunil Kumar, Ravinder Nagpal, Amit Kumar, Muhammad Umer Ashraf, Yong-Soo Bae
Summary: Epigenetic alterations, particularly in the form of RNA modifications like m6A, show therapeutic potential in managing hematological disorders, such as acute myeloid leukemia (AML). Further research into understanding and utilizing RNA modifiers could lead to the development of novel therapeutics for leukemia and other cancers.
Meeting Abstract
Rheumatology
D. Wendling, W. Abbas, A. Kumar, S. Pasquereau, F. Verhoeven, C. Prati, C. Demougeot, G. Herbein
ANNALS OF THE RHEUMATIC DISEASES
(2018)