Article
Biotechnology & Applied Microbiology
Maria Papadatou, Mollie Knight, Maria Salta
Summary: In the maritime field, quantification methods of biofilm development are crucial due to the economic and environmental impacts of biofouling. This study developed a method using phototrophic biofilms and monoculture biofilms to test artificial polymeric surfaces in situ and in vitro, and verified its strong correlation with traditional quantification techniques. The results demonstrated the efficiency of using inherent chlorophyll fluorescence for quantifying undisturbed phototrophic biofilms.
Article
Microbiology
Hui Chen, Kunfeng Li, Chunling Xue, Qiang Wang
Summary: Researchers have developed a novel method using chlorophyll fluorescence induced dynamic (OJIP) curve to estimate Chl a content and photosynthetic microalgal cell density in water samples. Experimental results show strong linear relationships between OJIP curve integrals and Chl a contents and cell densities for various microalgal cultures and natural communities.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Laura Pilar Gomez Barrio, Eduarda Melo Cabral, Ming Zhao, Carlos Alvarez Garcia, Ramsankar Senthamaraikannan, Ramesh Babu Padamati, Uma Tiwari, James Francis Curtin, Brijesh Kumar Tiwari
Summary: This study optimized the extraction process of agar using response surface methodology, improving extraction efficiency by reducing processing time and energy consumption. Ultrasound-assisted extraction was found to further enhance the extraction efficiency compared to conventional extraction. The extracted agar showed similar spectra to commercial agar, but had lower gel strength, indicating potential novel applications.
Article
Environmental Sciences
Helena Svecova, Andrea Vojs Stanova, Ales Klement, Radka Kodesova, Roman Grabic
Summary: Global climate changes have led to water scarcity in many regions, making the sustainable use of recycled water crucial in agriculture. However, the presence of potentially hazardous compounds like pharmaceuticals in crops can pose significant risks. This study aims to investigate the presence of selected pharmaceutical active compounds (PhACs) and their metabolites in aeroponically grown crops and assess the potential for plant uptake of these compounds.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Microbiology
Linlin Zhang, Jie Liu, Xin Shen, Shuangwei Li, Wenfang Li, Xinfeng Xiao
Summary: This study optimized the conditions for chlorophyll a (Chl a) extraction from the microalga Isochrysis galbana. The key parameters affecting Chl a content were determined through a single-factor optimization experiment, and then the interaction of three factors (salinity, pH, and nitrogen concentration) was optimized using the Box-Benhnken Design. The artificial intelligence models (ANN, SVM, and RBFNN) showed better prediction capability for Chl a content than the traditional response surfaces method (RSM).
Article
Chemistry, Medicinal
Ga-Hyun Joe, Masafumi Masuoka, Ryosuke Reisen, Seiya Tanaka, Hiroki Saeki
Summary: A separation process was developed to fractionate and recover three anti-inflammatory components from the red alga P. palmata, including sugars, phycobiliprotein, and chlorophyll. The process involved three steps without the use of organic solvents. The extracts obtained from each step, rich in sugars (E1), phycobiliprotein-derived peptides (E2), and chlorophyll-related components (E3), showed anti-inflammatory activity without any negative effects.
Article
Biochemistry & Molecular Biology
Nancy Lara-Almazan, Graciela Zarazua-Ortega, Pedro Avila-Perez, Carlos Eduardo Barrera-Diaz, Alberto Cedillo-Cruz
Summary: Phytochelatins are peptides in plants that help maintain homeostasis and detoxify heavy metals. Researchers have proposed them as potential biomarkers for aquatic pollution. A new method using UPLC-MS was validated for accurate and precise quantification of PCs in aquatic plants, with excellent linearity and precision.
Article
Multidisciplinary Sciences
Elise Albert, Sungsoo Kim, Maria Magallanes-Lundback, Yan Bao, Nicholas Deason, Benoit Danilo, Di Wu, Xiaowei Li, Joshua C. Wood, Nolan Bornowski, Michael A. Gore, C. Robin Buell, Dean DellaPenna
Summary: The tocopherol biosynthetic pathway in plants is encoded by VTE genes, but many quantitative trait loci for seed total tocopherols do not have VTE genes, suggesting other activities are involved. A study on Arabidopsis seed tocopherols found a significant interval without VTE genes, which mapped to an uncharacterized, seed-specific, envelope-localized, alpha/beta hydrolase. Mutations in AtVTE7 and its maize ortholog ZmVTE7 significantly decreased seed and leaf total tocopherols, while overexpression of these genes increased leaf totaltocopherols. These findings suggest a model where VTE7 releases prenyl alcohols from chlorophyll biosynthetic intermediates for tocopherol biosynthesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Plant Sciences
Antoine Firmin, Marc-Andre Selosse, Christophe Dunand, Arnaud Elger
Summary: Aquatic Embryophytes play a crucial role in aquatic ecosystems and carbon is vital for biogeochemical processes. The phenomenon of mixotrophy, where autotrophs directly use exogenous organic carbon, is widespread among aquatic plants and is more common than previously believed.
TRENDS IN PLANT SCIENCE
(2022)
Article
Biochemical Research Methods
Yunyu Tang, Guangxin Yang, Yingqing Ma, Dongmei Huang, Wenlei Zhai, Essy Kouadio Fodjo, Xuan Zhang, Siman Li, Weiyi Zhang, Yongfu Shi, Cong Kong
Summary: In this study, a sensitive and validated HPLC-MS/MS method was developed for the determination of moenomycin A in aquatic animal products. The method exhibited good linearity, low detection limit, and was effectively applied in different aquatic samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Ibrahim A. Senosy, Zhi-Heng Lu, Dong-Dong Zhou, Talat M. Abdelrahman, Min Chen, Lv-Yun Zhuang, Xiao Liu, Yi-Wen Cao, Jian-Hong Li, Zhong-Hua Yang
Summary: A sensitive and cost-effective method for the quantitative analysis of azole pesticides residues in six medicinal plants was established using magnetic cyclodextrin crosslinked with tetrafluoroterephthalonitrile (Fe3O4@TFN-CDPs) coupled with high-performance liquid chromatography (HPLC). This method provides a new idea for the analysis of trace pesticides in complicated matrices.
Article
Chemistry, Multidisciplinary
Nicolae Marinel Horablaga, Antoanela Cozma, Ersilia Alexa, Diana Obistioiu, Ileana Cocan, Mariana-Atena Poiana, Dacian Lalescu, Georgeta Pop, Ilinca Merima Imbrea, Ciprian Buzna
Summary: This paper evaluates the influence of preparation and extraction methods on the phytochemical profile and microbiological activity of 12 medicinal plants from the west side of Romania. The study found that the sample preparation method (shredding or grinding) affects the content of phytocompounds, with increased levels in powder form. The use of green technologies, particularly microwave extraction, resulted in the highest levels of total polyphenol content, flavonoid content, and antioxidant activity. The optimized extracts showed significant antimicrobial potential, particularly against certain strains of bacteria and fungi.
APPLIED SCIENCES-BASEL
(2023)
Article
Marine & Freshwater Biology
Maha Krayem, S. El Khatib, Yara Hassan, Veronique Deluchat, Pascal Labrousse
Summary: The review discusses the toxic effects of Cu on plant growth and development, with a special focus on aquatic plants. While Cu is considered essential for vital mechanisms in plants, excess levels can lead to harmful effects, including alterations in biochemical, physiological, and morphological aspects. Additionally, Cu has detrimental effects on DNA and hormonal balance.
AQUATIC TOXICOLOGY
(2021)
Article
Engineering, Civil
An Xu, Shumin Li, Jiyang Fu, Anil Misra, Ruohong Zhao
Summary: Frame structure optimization is achieved by integrating continuum topology optimization, transformation process, and size optimization. It starts with continuum topology optimization to get a rough layout of structural members, then transforms it into a frame structure using non-curved and prismatic members for easier construction. Finally, member sizes are optimized to obtain the optimal design. This hybrid method significantly reduces computational intensity compared to traditional discrete optimization methods.
ENGINEERING STRUCTURES
(2023)
Article
Multidisciplinary Sciences
Siti Hajar Abd Rahim, Norazwina Zainol, Kamaliah Abdul Samad
Summary: This research aims to optimize the conditions of chlorophyll extraction from pineapple leaves using a mechanical extraction method. It has been established that the models are suitable for optimizing the extraction conditions in order to maximize the concentration of total chlorophyll (TC) using the mechanical method.
Article
Fisheries
Xianyong Bu, Ying Song, Jingyu Pan, Xiaodan Wang, Chuanjie Qin, Yongyi Jia, Zhenyu Du, Jian G. Qin, Liqiao Chen
Summary: The study found that dietary supplementation of myo-inositol (MI) can effectively alleviate the negative effects of chronic copper exposure on Chinese mitten crab E. sinensis by improving oxidative stress, immune and hepatopancreas damage, mitochondrial dysfunction, apoptosis, and inflammatory responses.
Article
Fisheries
Qiuran Yu, Fenglu Han, Maoxian Huang, Xiaodan Wang, Jian G. Qin, Liqiao Chen, Erchao Li
Summary: This study found that different doses of myo-inositol can significantly affect the growth performance, body composition, serum metabolite composition, hepatopancreatic metabolite content, and digestive enzyme activity in juvenile Pacific white shrimp. It suggests that myo-inositol can promote shrimp growth by improving lipid utilization.
Article
Environmental Sciences
Yiwen Yang, Qiuran Yu, Cong Zhang, Xiaodan Wang, Long He, Yuxing Huang, Erchao Li, Jianguang Qin, Liqiao Chen
Summary: The study found that thiamethoxam, a neonicotinoid insecticide, can be toxic to crustaceans, leading to hepatotoxicity and neurotoxic effects. The toxicity is related to the activation of antioxidant and detoxification systems, regulation of immune-related gene expression, and potential inflammatory response. High concentrations of thiamethoxam also cause decreased acetylcholinesterase activity and downregulation of synaptic transmission-related genes, affecting neurotoxicity in crustaceans.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Jiadai Liu, Cong Zhang, Xiaodan Wang, Xinyu Li, Qincheng Huang, Han Wang, Yixin Miao, Erchao Li, Jianguang Qin, Liqiao Chen
Summary: This study investigated the potential of high dietary methionine levels in alleviating chronic heat stress in Chinese mitten crab Eriocheir sinensis. The results showed that under high water temperatures, the crabs exhibited decreased feed conversion ratio and increased weight gain, specific growth rate, molting frequency, and protein efficiency ratio. However, survival rate decreased. The high-methionine diet was found to reduce lipid accumulation and increase protein content, while also mitigating oxidative stress and activating immune function.
Article
Fisheries
Jingyu Pan, Liqiao Chen, Yuzhou Ji, Yuxing Huang, Xianyong Bu, Jiahua Zhu, Erchao Li, Jianguang Qin, Xiaodan Wang
Summary: This study aimed to investigate the relationship between inositol metabolism and carbohydrate metabolism in Nile tilapia under acute hyperosmotic stress. Glucose solution and PBS were injected into the experimental and control group fish, respectively, followed by exposing them to brackish water for different time points. The hyperosmotic stress increased osmotic pressure, glucose content, and metabolic processes in the liver of fish. Genes related to inositol synthesis and transport were induced by hypertonicity in gill, kidney, and liver tissues. Glucose injection decreased inositol synthesis and apoptosis in the liver, but increased Na+K+-ATPase activity and osmoregulation ability in the gill.
AQUACULTURE REPORTS
(2023)
Article
Fisheries
Qincheng Huang, Xiaodan Wang, Zhideng Lin, Jiadai Liu, Han Wang, Cong Zhang, Zhenyu Du, Jianguang Qin, Liqiao Chen
Summary: The present study investigated the effect of vitamin A on growth performance, lipid metabolism, fatty acid composition, antioxidant ability and inflammatory response of crabs fed different vegetable oils. The interaction between vitamin A and lipid source did not influence the survival rate, final body weight, weight gain and specific growth rate of crabs. Crabs fed perilla oil showed optimal growth performance with the lowest body lipid content and hemolymph triglyceride, while crabs fed coconut oil showed the worst growth performance and aggravating inflammation. Dietary vitamin A improved growth performance and relieved inflammation of crabs fed different lipid sources, enhancing antioxidant activity and mitigating lipid peroxidation.
Article
Fisheries
Xianyong Bu, Qincheng Huang, Ying Song, Changle Qi, Zhideng Lin, Xiaodan Wang, Meiling Zhang, Chuanjie Qin, Jian G. Qin, Liqiao Chen
Summary: This study evaluates the effects of dietary myo-inositol (MI) on the ovarian development of female Chinese mitten crab (Eriocheir sinensis). The results showed that feeding crabs diets supplemented with 1600 and 3200 mg/kg MI significantly promoted ovarian development, increased gonadosomatic index and yolk granule deposition. MI supplementation also enhanced vitellogenin synthesis, lipid metabolism, and transport-related gene expression in the hepatopancreas and ovary. Thus, feeding a diet with 1600mg/kg MI can effectively promote ovarian development in Chinese mitten crabs.
Article
Fisheries
Ying Song, Xinyu Cai, Xianyong Bu, Shubin Liu, Mingqi Song, Yiwen Yang, Xiaodan Wang, Qingchao Shi, Jianguang Qin, Liqiao Chen
Summary: This study evaluates the effects of dietary iron and vitamin C on the growth performance, iron utilization, antioxidant capacity, and nonspecific immunity of the Chinese mitten crab. The results show that dietary supplementation of 700.90 mg/kg of vitamin C can improve iron absorption and utilization, thereby increasing the growth, immunity, and disease resistance of the crab.
AQUACULTURE NUTRITION
(2023)
Article
Fisheries
Cong Zhang, Jiadai Liu, Xiaodan Wang, Erchao Li, Mingqi Song, Yiwen Yang, Chuanjie Qin, Jianguang Qin, Liqiao Chen
Summary: Previous research has shown that hypoxia can be harmful to the neural system of Chinese mitten crabs, but this study found that GABA can provide neuroprotection against hypoxia-induced damage. Through feeding and hypoxia challenge experiments, it was discovered that GABA can protect the neural system of Chinese mitten crabs and regulate their metabolism.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Chemistry, Medicinal
Trung T. T. Nguyen, Thanh Hoang, Tuyet Pham, Vi Khanh Truong, Xuan Luo, Jian Qin, Wei Zhang
Summary: Shell wastes impose burdens on the shellfish industry, but they can be used for commercial chitin production to minimize negative impacts and maximize economic value.
Article
Ecology
Shirin Akter, Melissa L. Wos-Oxley, Sarah R. Catalano, Md Mahbubul Hassan, Xiaoxu Li, Jian G. Qin, Andrew P. A. Oxley
Summary: Pacific oysters and Mediterranean mussels have gut microbiota that plays an important role in their health and nutrition. The composition of their gut microbiota is influenced by both the host and the environment, as revealed by studying their bacterial assemblages in seawater and gut samples.
Article
Agriculture, Dairy & Animal Science
Jiahua Zhu, Fan Zhang, Yuxing Huang, Jingyu Pan, Erchao Li, Jian G. Qin, Liqiao Chen, Xiaodan Wang
Summary: This research explores the influence of myo-inositol on the hypertonic stress adaptation of Nile tilapia by increasing lipid utilization and improving osmotic regulation ability. It was found that myo-inositol supplementation improved growth performance, promoted protein deposition, reduced fat accumulation, and alleviated liver lipid accumulation. Additionally, it reduced serum lipids and upregulated the expression of ion transporter-related genes. The findings suggest that dietary supplementation with myo-inositol can regulate lipid metabolism and improve the physiological response of Nile tilapia to hypertonic stress.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2023)
Article
Fisheries
Xiaochen Zhu, Penny Miller-Ezzy, Mark Gluis, Yingying Zhao, Jianguang Qin, Youhong Tang, Yibing Liu, Xiaoxu Li
Summary: Pacific oysters are important in aquaculture, and this study investigated how to improve larval cryopreservation techniques. The results showed that adding the lipid POPC to the base CPA significantly increased larval development after thawing. Adding tocopherol alone had no significant effect. However, combining POPC and tocopherol improved survival rates and doubled the spat production compared to the base CPA.
Article
Fisheries
Yang Zhou, Li Xu, Zhongtang He, Weijie Cui, Qun Lu, Jianguang Qin, Shengqi Su, Tao He
Summary: This study successfully differentiated different stocks of Schizothorax grahami in the Chishui River by analyzing otolith morphology, providing important evidence for the scientific management of these fish resources.
Article
Fisheries
Xiaochen Zhu, Penny Miller-Ezzy, Mark Gluis, Jianguang Qin, Youhong Tang, Yibing Liu, Yingying Zhao, Xiaoxu Li
Summary: This study investigated the effect of adding lipids and antioxidants on the survival rate of frozen mussel oocytes. The results showed that supplementing phospholipids or antioxidants in the base cryoprotectant agent significantly increased the survival rate of mussel larvae. Additionally, adding salmon lipid extract resulted in the highest survival rate and increased the number of mussel spat.
